The purpose of this study was to investigate the effects of relating factors on the nutrient intakes in elderly people aged over 85 years residing Namhae Kyungnam. The subject of this study was composed of 24 males and 76 females, the average age being 88.9+/-4.0 years old. The consumption of energy and most other nutrients was higher in females than males. There were significant positive correlations the education level, pocket money, self-perception of health and happiness, frequency and regularity of meal with nutrient intakes in elderly males and females. The smoking was negatively correlated with protein and niacin intakes(p<0.05). The alcohol drinking and sleeping hours were not significantly correlated with nutrient intakes.
Aged* ; Alcohol Drinking ; Education ; Female ; Gyeongsangnam-do ; Happiness ; Humans ; Male ; Meals ; Niacin ; Self Concept ; Smoke ; Smoking

The purpose of this study was investigating serum lipid, apolipoprotein levels and their correlations in healthy adults of Gyeongnam area. The BMI (body mass index) was significantly higher (p < 0.001) in male (25.2 +/- 2.7 kg/m2) than female (23.8 +/- 1.5 kg/m2), however PBF (percent body fat) was significantly higher (p < 0.001) in female (29.6 +/- 4.3%) than male (22.7 +/- 5.0%). The WHR (waist to hip ratio) and blood pressure in the groups showed there was no significant differences. The levels of serum total cholesterol, LDL-cholesterol and apolipoprotein B were significantly higher (p < 0.01) in male (208.7 +/- 27.7 mg/dl, 129.0 +/- 26.9 mg/dl, 1.0 +/- 0.2 g/L) than female (193.6 +/- 29.1 mg/dl, 112.5 +/- 29.5 mg/dl, 0.9 +/- 0.2 g/L, but HDL-cholesterol level was significantly higher (p < 0.01) in female (54.9 +/- 6.6 mg/dl) than male (49.9 +/- 7.3 mg/dl). The LDL-C/HDL-C, Apo B/Apo A-I and AI (atherogenic index) were significantly higher (p < 0.001) in male (2.6 +/- 0.6, 0.8 +/- 0.2, 3.3 +/- 0.7) than female (2.1 +/- 0.5, 0.6 +/- 0.2, 2.6 +/- 0.5). The triglyceride level was positively correlated with apolipoprotein B concentration (p < 0.05) and negatively correlated with HDL-cholesterol concentration (p < 0.05), however no significant correlation was found with apolipoprotein A-I. According to these results, we conclude that male adults are expecting higher incidence of cardiovascular disease than female adults and we suggest the serum triglyceride should be kept normal level for the prevention of these diseases.
Adult* ; Apolipoprotein A-I ; Apolipoproteins* ; Blood Pressure ; Cardiovascular Diseases ; Cholesterol ; Female ; Hip ; Humans ; Incidence ; Male ; Triglycerides

Human dermal fibroblast is essential in wound healing of the skin through the synthesis of extracellular matrix proteins. With respect to oxidative stress, the effects of remifentanil on human dermal fibroblast have received little attention. Therefore, we investigated the effects of remifentanil on the apoptosis and autophagic reaction of human dermal fibroblasts under oxidative stress. The subjects were divided into the following groups: Control group: cells were incubated at 37℃ in a humidified atmosphere with 5% CO₂. Hydrogen peroxide (H₂O₂) group: cells were exposed to H₂O₂ for 2 h. RPC/H₂O₂ group: cells were pretreated with remifentanil for 2 h and exposed H₂O₂ for 2 h. 3-MA/RPC/H₂O₂ group: cells were pretreated with 3-methyladenine (3-MA) and remifentanil for 1 h and 2 h, respectively. We measured cell viability using MTT assay. Western blot analysis was used to determine the expression levels of proteins associated with apoptosis and autophagy. Quantification of apoptotic cells was performed using flow cytometer analysis, and autophagic vacuoles were observed under a fluorescence microscope. Remifentanil treatment increased the proliferation of human dermal fibroblast and decreased apoptotic cell death, enhancing autophagic activity under oxidative stress. However, 3-MA, the autophagy pathway inhibitor, inhibited the protective effect of remifentanil in oxidative stress. This study demonstrates that remifentanil activated autophagy and decreased apoptotic death of human dermal fibroblasts under oxidative stress. Our results suggest that remifentanil may help in the treatment of oxidative stress.
Apoptosis ; Atmosphere ; Autophagy ; Blotting, Western ; Cell Death ; Cell Survival ; Extracellular Matrix Proteins ; Fibroblasts* ; Fluorescence ; Humans* ; Hydrogen Peroxide ; Oxidative Stress* ; Skin ; Vacuoles ; Wound Healing

Remifentanil is commonly used in operating rooms and intensive care units for the purpose of anesthesia and sedation or analgesia. Although remifentanil may significantly affect the bone regeneration process in patients, there have been few studies to date on the effects of remifentanil on bone physiology. The purpose of this study was to investigate the effects of remifentanil on osteoclast differentiation and bone resorption. Bone marrow-derived macrophages (BMMs) were cultured for 4 days in remifentanil concentrations ranging from 0 to 100 ng/ml, macrophage colony-stimulating factor (M-CSF) alone, or in osteoclastogenic medium to induce the production of mature osteoclasts. To determine the degree of osteoclast maturity, tartrate-resistant acid phosphatase (TRAP) staining was performed. RT-PCR and western blotting analyses were used to determine the effect of remifentanil on the signaling pathways involved in osteoclast differentiation and maturation. Bone resorption and migration of BMMs were analyzed to determine the osteoclastic activity. Remifentanil reduced the number and size of osteoclasts and the formation of TRAP-positive multinuclear osteoclasts in a dose-dependent manner. Expression of c-Fos and NFATC1 was most strongly decreased in the presence of RANKL and remifentanil, and the activity of ERK was also inhibited by remifentanil. In the bone resorption assay, remifentanil reduced bone resorption and did not significantly affect cell migration. This study shows that remifentanil inhibits the differentiation and maturation of osteoclasts and reduces bone resorption.
Acid Phosphatase ; Analgesia ; Anesthesia ; Blotting, Western ; Bone Regeneration ; Bone Resorption* ; Cell Movement ; Humans ; Intensive Care Units ; Macrophage Colony-Stimulating Factor ; Macrophages ; Operating Rooms ; Osteoclasts* ; Physiology