1.Influence of Pathogen Inactivation on the Quality of Platelet Rich Plasma Derived Platelets.
So Yong KWON ; Jae Won KANG ; Youn Jung CHO ; Nam Sun CHO ; Gy Youl NAM ; Yoon Hwan CHANG ; Tae Hee HAN ; Sang Won LEE
Korean Journal of Blood Transfusion 2013;24(2):128-139
BACKGROUND: Pathogen inactivation (PI) is a proactive approach to overcome the limitations of the current testing system and donor questionnaires. Effect of PI on non-leukoreduced platelet rich plasma derived platelets (PRP-PLTs) suspended in plasma has not yet been evaluated. This study was conducted in order to evaluate the effect of PI on the quality of non-leukoreduced PRP-PLTs suspended in plasma. METHODS: PRP-PLTs treated with the Mirasol PRT System and the Intercept Blood System were tested for PLT count, blood gas, PLT activation, and apoptosis on days 3, 5, and 7 of storage. RESULTS: PLT number showed a decrease after PI. No difference in pH was observed until day 5. At day 7, PLTs treated with Mirasol had a lower pH value (6.5), however, it satisfied the quality control criteria. PLTs treated with Mirasol had a lower pO2 compared to pre-inactivation PLTs. pO2 during storage differed significantly between the two PI groups. pCO2 showed a decrease after inactivation and both groups showed a significant difference, compared with the control. PLTs treated with Mirasol had increased P-selectin expression after inactivation; however, difference of P-selectin during storage was not significant compared to the control. P-selectin of PLTs treated with Intercept was significantly different compared to those treated with Mirasol and control. Annexin V showed an increase after inactivation in Mirasol treated PLTs and difference during storage was significant compared to control and Intercept. CONCLUSION: As both PI systems showed satisfactory pH values, the criteria showing a high correlation with in vivo PLT viability and generally applied to monitor quality of PLTs, quality of PRP-PLTs after PI appears not to be negatively affected.
Annexin A5
;
Apoptosis
;
Blood Platelets
;
Humans
;
Hydrogen-Ion Concentration
;
Organothiophosphorus Compounds
;
P-Selectin
;
Plasma
;
Platelet-Rich Plasma
;
Quality Control
;
Tissue Donors
;
Surveys and Questionnaires