OBJECTIVE:To provide a reference for promoting the effect of government subsidies to the R&D conducted by pharmaceutical enterprises,increasing enterprises’internal input to R&D and improving innovation performance. METHODS:By building the theoretical model of the relation of the government subsidies and internal input to R&D and the innovation performance on the basis of the assumptions,the empirical study was conducted on the pharmaceutical enterprises in Jiangsu Province though re-gression analysis and mediating effect analysis. RESULTS & CONCLUSIONS:The scale designed had good reliability and validity. The final model was obtained after empirical test. Giving considerations to relevant coefficients,the results showed that all assump-tions were verified except 3. Where,government subsidies to the R&D promoted enterprises’internal input to R&D and innovation performance,and internal input to R&D served as a complete mediator between government subsidies to the R&D and innovation performance,that is to say,government subsidies to the R&D had indirect promotion influence on innovation performance,with the influence coefficient as 0.405. It is suggested that the government should increase subsidies to R&D conducted by pharmaceuti-cal enterprises in a reasonable manner to make full use of the guiding function of government funds,and pharmaceutical enterprises should attach importance to internal input to R&D and establish its principal status in innovation system.

The change of plasma TXB_2 and 6-Keto-PGF_1? which were stable metabolites of TXA_2 and PGI_2 respectively were observed in arterial blood of SHR and WKY and in different ages of SHR. The 6-Keto/TXB_2 ratios were computed. The results showed that TXB2 concentration of arterial blood were significantly higher in SHR than in WKY(1217.9?298.5Pg/ml vs 207.1?59.8Pg/ml, P

OBJECTIVE:To study the application of CRM system in pharmaceutical industry METHODS:To introduce the conception and application software of CRM system and analyse the problems of CRM system in pharmaceutical industry RESULTS & CONCLUSION:The application of CRM system can help pharmaceutical enterprises to raising the core competitive ability

Objective To survey the unconventional blood sugar level induced by quinolones and promote the rational drug use in clinical. Methods Collecting and analysis the unconventional blood sugar level induced by quinolones reported during the period of 1995 ～ 2008 using CHKD net. Results 107 cases of unconventional blood sugar level were reported by using 9 kinds of quinolones and among them 70 cases with hypoglycemia and 3 cases died of hypoglycemic coma. Another 37 cases were found with hyperglycemia. Conclusion Should reinforce the supervi-sion of rational use of quinolones and monitor the blood sugar level when the elderly and the diabetic of renal insuffi-ciency in clinical use.

Objective:To explore the clinical effect of Qizhi Weitong granules combined with rabeprazole in the treatment of reflux esophagitis and the influence on the levels of gastrin and motilin. Methods:Totally 80 cases of patients with reflux esophagitis were se-lected, and then randomly divided into the observation group and the control group with 40 cases in each. The control group was treated with rabeprazole, and the observation group was treated with Qizhi Weitong granules additionally. After 4-week treatment, the clinical effect and the influence on the levels of gastrin and motilin in the two groups were compared. Results:By comparing the clinical effect and endoscopic curative in the two groups, the total effective rate of the observation group was 95. 0% and 92. 5%, which was obvious-ly higher than that of the control group. Compared with those before the treatment, the levels of gastrin and motilin in the two groups were significantly improved after the treatment, and the improvement of the observation group was obviously higher than that of the con-trol group with statistically significant difference (P<0. 05). Conclusion: Qizhi Weitong granules combined with rabeprazole in the treatment of reflux esophagitis is effective, which can greatly improve the levels of gastrin and motilin and is worthy of further clinical application.

Objective To obtain the prevalence data on human T Lymphotropic virus (HTLV) infection in 2 339 blood donors of an endemic coastal region of Fujian, China. Methods Serum antibodies to HTLV in the donors were detected by a home made double antigen sandwitch ELISA kit. All the ELISA positive samples were further confirmed by Western blot (WB) and/or polymerase chain reaction (PCR) combined with sequencing. Results Nine samples were confirmed as HTLV 1 infection among the 2 339 donors. A man whose wife is one of the nine was also detected to infect by WB and PCR. Conclusion The prevalence of HTLV infection was 0.38% in the endemic coastal region of Fujian, China.

Haemophilus influenzae (Hi) is a pathogen exclusively found in humans. It causes a wide range of infections from the upper respiratory tract to serious invasive diseases. Such as pneumonia, septicemia and meningitis. Strains of Hi are usually classif ied into six serotypes a to f and nontypeable H. influenzae (NTHI) according to the antigenicities and compositions of their polysaccharide capsules. Hib was a common cause of serious infections in younger children. The polysaccharide-protein conjugate vaccines against Hib had almost eliminated H. influenzae as a cause of pediatric meningitis. However, NTHI remains an important pathogen, particularly in children and the elderly. Efforts to understand and control NTHI disease have been hampered by the diversity of these bacteria. This review introduced the study progress about pathogenic mechanism of NTHI. In order to provide the help for development of vaccine, clinic treatment and prevent the occurrence of diseases causing by NTHI.

Objective To investigate the roles of reactive oxygen species (ROS) and phosphatidyl-inositol 3-kinase-Akt (PI3K-Akt) in neuroprotection against spinal cord ischemia-reperfusion (I/R) injury by ischemic postconditioning (IP) or controlled low perfusion pressure (LR). Methods One hundred and twenty-six adult male SD rats weighing 300-350 g were randomly divided into 7 groups (n = 18 each): group Ⅰ I/R; group ⅡIP; group Ⅲ LR; group Ⅳ IP + LY-294002 (IP + LY); group Ⅴ LR + LY-294002 (LR + LY); group Ⅵ IP +N-acetylcysteine (IP+ N) and group Ⅶ LR+ N-acetyl-cysteine (LR+ N). Spinal cord ischemia was induced by 9 min occlusion of the thoracic aorta combined with controlled hypotension (MAP = 40 mm Hg). In IP group the animals were subjected to 3 cycles of 30 s reperfusion interspersed with 30 s ischemia immediately after release of aortic occlusion. In LR group MAP was maintained at 40 mm Hg for another 5 min immediately after release of aortic occlusion, then increased to 100 mm Hg. In group Ⅳ-Ⅶ LY-294002 (PI3K inhibitor) 25 mg/kg or N-acetylcysteine (ROS scavenger) 100 mg/kg were administered at the onset of reperfusion. Twelve animals in each group were killed at 2 h of reperfusion. The lumbar segment of the spinal cord was removed for determination of the level of Akt phosphorylation and opening of mitochondrial permeability transition pore (mPTP). Neurological function was assessed and scored (15 = normal function, 0 = unable to move hind limb) at 4, 12, 24 and 48 h of reperfusion in another 6 animals in each group. The animals were then killed after last assessment and the lumbar segment of the spinal cord was removed for detection of apoptotic neurons. Results Compared with I/R group,both IP and LR significantly enhanced the level of Akt phosphorylation in the spinal cord, inhibited mPTP opening and neuronal apoptosis and increased neurological function scores. There was no significant difference in the protective effects exerted by IP and LR. The neuroprotective effects exerted by IP and LR were abolished by LY-294002 and N-acetylcysteine. Conclusion Ischemic postconditioning or controlled low perfusion pressure can protect the spinal cord from I/R injury by activating PI3K-Akt and inhibiting mitochondrial permeability.

The excitability of cerebral cortex will change after the onset of ischemic stroke.Repetitive transcranial magnetic stimulation (rTMS) can be used to regulate the excitability in cerebral hemisphere cortex and promote the motor function recovery in patients with ischemic stroke.Studies have shown that both the affected hemisphere low-frequency rTMS and the unaffected hemisphere high-frequency rTMS can promote the motor function recovery in patients with ischemic stroke.The former may be more effective than the

AIM: To observe the changes in infiltrating macrophages (M?)and extracellular matrix (ECM) in the kidney in the progressive course of nephrotoxic nephritis (NTN). METHODS: NTN model was established with rabbit-anti-rat nephrotoxic serum. On day 3, 7, 15, 30 and 90, renal biopsies were performed. Renal histology was checked under light microscopy with HE and Masson's trichrome staining sections. M?, fibronectin (FN), type Ⅲ and Ⅳ collagen were examined with immunohistochemistry ABC method. RESULTS: Infiltration of M? appeared on day 3 of NTN and preceded changes of FN and collagen. On day 15 of NTN, all nephritic animals had significant proteinuria, increased serum creatinine, infiltration of M? and deposit of entracellular matrix. On day 90 of NTN, seven nephritic animals improved significantly, while other five developed renal scarring with diffuse infiltration of M? which positively paralleled to renal function and deposit of FN, type Ⅲ and Ⅳ collagen. CONCLUSION: M? infiltrating into renal tissue enhances deposit of ECM and therefore plays important roles in progression or improvement of NTN.