BACKGROUND: There is no effective method to identify liver cancer stem cells until now, which has become one of the major challenges in this field. OBJECTIVE: To explore a more effective and suitable way for the identification of liver cancer stem cells in hepatocellular carcinoma cell lines. METHODS: Firstly, the single cell separation technique was used to obtain single cells, which were seeded into 96-wel plates one by one. Secondly, cell sublines derived from single cell colonies (tumorigenic colonies) were selected and obtained. At last, the cells from these clones were transplanted into the forelimb armpits of nude mice to observe the tumorigenic ability. RESULTS AND CONCLUSION: The number of holes of single cells obtained was 371 by the single cell separation technique, and the success rate was 96.4%. According to the growth of cell clone, tumorigenic colonies were selected to be transplanted to the forelimb armpits of nude mice, and the tumor formation rate of the colonies was 100%. The identification model of single-cell-transplant-tumorigenic-test for liver cancer stem cells is confirmed to be preliminarily established, which lays the technology and methodological basis for the follow-up research.

BACKGROUND:Until now, little has been reported on establishment of pancreatic stem cell strains and lines,and the purification of pancreatic stem cells is difficult since the cell line establish rate is low.OBJECTIVE:To explore a more rational and effective technique of in vitro separation and continuous passage of pancreatic stem cells, with the hope to establish cell strains and even cell lines and to lay the foundation for the follow-up study of pancreatic stem cells in the treatment of diabetes.METHODS:Firstly, Percoll discontinuous density gradient centrifugation method was applied to separate the mouse pancreatic endocrine portion from the exocrine portion, then to obtain cell strains with highly proliferative ability and low differentiation from pancreatic endocrine portion-the islet. We used mouse embryonic fibroblasts treated with mitomycin C as a feeder layer, for in vitro continuous culture of islet-derived pancreatic stem cells under feeder layer conditions until they were transferred to the 30th passage to establish cell lines. Then pancreatic stem cell line derived from pancreatic islet was detected and identified by a series of tests including growth characteristic test, morphological observation, related molecular marker identification and differentiation characteristic identification.RESULTS AND CONCLUSION:In the continuous process of passage, pancreatic stem cells showed active proliferative ability, and maintained the typical morphological characteristics of stem cells and expression of pancreatic stem cell marker-Nestin. After induction, pancreatic stem cells showed insulin gene expression,reflecting their differentiation potential. Therefore, under the condition of feeder layer, the pancreatic stem cell line derived from Kunming mice was successfully established and the related identification was completed,which lays the foundation for the following research.

Objective:To analyze the research status and hotspots of patients with heart failure at home and abroad, to provide a reference for the exercise rehabilitation research of heart failure patients in China.Methods:The research references on exercise rehabilitation for heart failure patients were retrieved in Web of Science Core Collection database and CNKI from 2011 to 2020 and were analyzed with the information visual software CiteSpace V 5.6 R5. The analysis included the annual distribution, country or institution distribution, journal distribution, author distribution, institute distribution, high-frequency keywords and forewords.Result:A total of 8 896 articles were included in the analysis, including 1 347 Chinese articles and 7 549 English articles. The number of international publications in exercise rehabilitation for patients with heart failure was much higher than that of domestic publications, and the overall trend was sustained growth. Among them, developed countries led by the United States (accounting for 36.415% of the total number of articles) occupy a leading position in exercise rehabilitation for heart failure patients. Foreign literature research hotspots mainly focus on telerehabilitation. In contrast, domestic literature research hotspots mainly included the effects of exercise rehabilitation on elderly patients with heart failure and the intervention of nursing staff in exercise rehabilitation. At the same time, both domestic and foreign paid attention to the effects of exercise rehabilitation on patients with heart failure such as exercise capacity, cardiac function, quality of life connection with myocardial infarction.Conclusions:The field of exercise rehabilitation for patients with heart failure in China has gradually received attention. However, there is a gap between the research and the international levels, so further research is still needed. Telerehabilitation abroad of patients with heart failure can provide references for the development of domestic research.

OBJECTIVESTo assess the culture and differentiation of neural stem cells in embryonic mice and set up a basis for further research in to neural stem cells.

METHODSEmbryonic cortices of mice were dissociated and single cell suspensions were achieved by mechanical methods in sterile conditions, and cells were seeded in uncoated plate in N2 medium. The cells were passaged by mechanical methods, frozen and thawed by general procedure. They were identified by immunocytochemical techniques.

RESULTSNeural stem cells from embryonic mice were successfully cultured forming typical neurospheres in suspension. Neurons, astrocytes and oligodendrocytes were differentiated from neural stem cells, with a ratio of 7%, 85% - 90% and 2% - 4% respectively.

CONCLUSIONSNeural stem cells, which can be cultured and passaged steadily in vitro and they are the ideal cell sources for cell transplantation and gene therapy.

Animals ; Cell Differentiation ; Cells, Cultured ; Embryo, Mammalian ; cytology ; Immunohistochemistry ; Mice ; Mice, Inbred BALB C ; Neurons ; cytology ; Stem Cells ; cytology

Objective:To evaluate and summarize the best evidence of exercise instructions for patients with atrial fibrillation.Methods:A comprehensive search about evidence on exercise rehabilitation in patients with atrial fibrillation was conducted in following databases: Joanna Briggs Institute (JBI) Library, The Agency for Healthcare Research and Quality (AHRQ), National Institute for Health and Clinic Excellence (NICE), Scottish Intercollegiate Guidelines Network (SIGN), Registered Nurses′ Association of Ontario (RNAO), Physiotherapy Evidence Database (PEDro), American College of Physicians (ACP), Cochrane Library, PubMed, Embase, CINAHL, Medlive, China National Knowledge Infrastructure (CNKI), WanFang Database, VIP Database, and China Biology Medicine (CBM). The retrieval period was from the inception of databases to January 2021. Two researchers with evidence-based nursing background assessed the quality of the literature independently and identified the level of evidence.Results:A total of 16 articles were included, including 4 guidelines, 4 systematic reviews, 4 expert consensuses, 4 randomized controlled trials. Combined with professional judgment, 35 pieces of best evidence in 7 aspects were summarized, including exercise benefits, the appropriate crowd, exercise evaluation, exercise mode, exercise intensity, exercise time, exercise supervision and safety.Conclusions:Regular exercise is safe and beneficial for patients with atrial fibrillation. Clinical staff should guide patients to exercise moderately by applying the best evidence with scientific exercise intervention, promoting patients′ physical and mental health.

Objective:To explore the current status of radiological protection knowledge, attitude and practice of nurses in Interventional Catheterization Room, and analyze their influencing factors so as to provide a basis for formulating intervention strategies.Methods:This study was a cross-sectional study. From April to May 2021, convenience sampling was adopted to select 240 nurses in Interventional Catheterization Room from 32 hospitals of member units of the China Heart Federation as the research subject. The self-designed General Information Questionnaire and the Radiological Protection Knowledge, Attitude and Practice Scale for Interventional Catheterization Room Nurses were used for investigation. Single factor analysis and multiple linear regression analysis were used to explore the influencing factors of radiological protection knowledge, attitude and practice among nurses of Interventional Catheterization Room. A total of 240 questionnaires were issued, and 220 valid questionnaires were returned with the valid response rate of 91.67%.Results:Among 220 nurses in Interventional Catheterization Room, the scores of radiological protection knowledge, attitude and practice dimensions were (5.92±1.83) , (29.15±1.97) , (43.76±5.73) , respectively. Multiple linear regression analysis showed that gender, hospital grade and training frequency were the influencing factors of radiological protection knowledge of nurses in Interventional Catheterization Room, and the differences were statistically significant ( P<0.05) . The training frequency and the type of radiological protective equipment were the influencing factors of the radiation protection attitude of nurses in Interventional Catheterization Room, and the differences were statistically significant ( P<0.05) . Gender, training frequency, regular monitoring of radiation dosimeters, and types of radiation protective equipment were the influencing factors of radiation protective practice of nurses in Interventional Catheterization Room ( P<0.05) . Conclusions:Interventional Catheterization Room nurses have a low level of radiological protection knowledge, and their attitudes towards radiological protection are relatively positive, but their radiological protection practice is not in place. The radiation protection training of nurses in Interventional Catheterization Room should be optimized, and adequate and high-quality protective equipment should be provided, and gender-specific management should be conducted, personal radiation dose monitoring should be standardized, and the radiation guidance role of Class Ⅲ hospitals should be used to ensure the safety and health of medical and nursing staff.

Objective:To explore the combination of study abroad and scientific research effectiveness, improve the " effectiveness concern" related to the selection mechanism for abroad-study, establish an evaluation system combined studying abroad and scientific research effectiveness.Methods:The research was based on 45 local-funded abroad-study programs, and used path analysis to connect main influence factors of studying abroad with scientific achievements, and establish a model.Results:The final path analysis model contained two regression equations: (1) in the equation of SCI articles, adjusted R2=0.600, F=5.698, P<0.001, statistic difference was significant. In this pathway, influence factors on SCI articles were successively Professional Title ( β=0.736, P<0.001), Age ( β=-0.700, P<0.001) and foreign institutions ( β=-0.318, P=0.031); (2) in the equation of Project funding, adjusted R2=0.490, F=7.608, P<0.001, statistic difference was significant. In this pathway, influence factors on project funding were successively SCI Articles ( β=0.409, P=0.010), Study country ( β=-0.342, P=0.015), and Degree ( β=-0.223, P=0.043). Conclusions:According to the path analysis, impacts of studying abroad on scientific research effectiveness were evaluated and detailed more precisely. The research established scientific-effectiveness oriented pathway between assigning and evaluation system, providing support to promote effectiveness of hospital abroad-study scientific outputs, and provide possible reference for local and national governments to optimize international exchange programs.