Various conditions were compared for extracting the proteolytic enxyme from the alimentary canal of Pneumatophorus juponicus (Houttuyn). The results showed that when using CaCl2 as activator, the suitable conditions were pH7. 5,extracting temperature 20 C,extracting time 2h,activiting time 16h,70% alcohol precipitaion.

Objective To analyzed the use of oral hypoglycemic drugs in our hospital for reference of clinical rational use of this kind of drugs. Methods The sorting methods of defined daily dose (DDDs) and the frequency of oral hypoglycenic drugs were used for analysis. Results The list of leading DDDs was glucosidase inhibitor, which accounted for 31.13 % of the total, followed by biguanides which accounted for 21.49%. Conclusions The glueosidase inhibitor, a new type oral hypoglycemic drugs used for lowering postprandial blood sugar level showed remarkable efficacy and safety, so it is currently the first choice for patients with diabetes.

Antiviral therapy is the most important treatment for chronic hepatitis C.This paper reviews the progress in antiviral treatment o-ver recent years,including the combination therapy with polyethylene glycol-Interferon (PEG-IFN)and ribavirin (RBV),specific target therapy,and gene therapy.The paper believes that the anti-hepatitis C virus treatment needs more effective drug combination therapies, shorter courses,less side effect,higher drug resistance threshold,etc.

Objective:To explore the effects of berberine hydrochloride on human periodontal ligament cells(HPDLCs) cultured in vitro. Methods:Cell proliferation activity was assayed by using MTT method. The total protein was detected by Coumassie Bright Blue method and the alkaline phosphatase (ALP) activities were measured by spectrophotometric assay. Results:①Comparing with the control group, the proliferative ability of PDLCs in berberine hydrochloride (0.005,0.010,0.020,0.030 g/L)groups at 24 h and (0.005,0.010,0.020 g/L)groups at 48 h and (0.010,0.020 g/L)groups at 72 h were considerably increased(P

Objective To investigate the clinical efficacy of adefovir dipivoxil in antiviral treatment of chronic hepatitis B and its effect on peripheral blood T lymphocyte subsets.Methods 40 cases of patients with chronic hepatitis B were selected from The Fourth People’s Hospital of Qinghai in October 2009 to June 2012,the patients were given the antiviral treatment of adefovir dipivoxil.Before treatment,3 months,6 months and 12 months after treatment,the changes of liver function indicators of alanine transaminase(ALT),aspartate transaminase(AST)and total bilirubin(TBiL),and hepatitis B virus-DNA(HBV-DNA)were recorded;the total effective rate was observed;the peripheral blood T lymphocyte subsets changes was tested and analyzed by flow cytometry.Results The ALT,AST and TBiL of the patients all improved good,and the indicators of ALT,AST and TBiL after 12 months decreased significantly than before treatment,the HBV-DNA testing of the patients recovered negative,the difference was statistically significant (P<0.05 );the total effective rate of hepatitis B patients is 92.5%;the peripheral blood T lymphocyte subsets has no significant improvement in patients after antiviral treatment for 3 months.After the treatment for 6 months and 12 months,the CD4 +T and CD4 +/CD8 +T were significantly higher than those before treatment,there was no significant changes in CD3 +T and CD8 +T before and after treatment.Conclusion Adefovir dipivoxil has a good clinical efficacy in antiviral treatment of chronic hepatitis B,and can significantly improve the patients’liver function,improve peripheral blood T lymphocyte subsets imbalance.

OBJECTIVE:To determine the antimicrobial activity of alcoholic e xtracts of47traditional Chinese herbal medicines in vitro.METHODS:Antibiotic drug sensitive tests of Escherichia coli and Bacillus pyocyaneus were conducted,al?coholic extracts of47Chinese herbal medicines were prepared,which were subjected to bicteriostatic tests in vitro by agar spread routine method.RESULTS:Among the47traditonal Chinese herbal medicines alcoholic extracts,11of which did have antimicrobial activity against escherichia coli,pseudomonas aeruginosa and blastomyces albicans in different degree;among which,the antimicrobial activity of the alcoholic extracts of paeoniae radix and granatum to drug resistance bacteria were the highest,the alcoholic extracts of paeoniae radix showed strong inhibitory activities to the above3bacterium,especially to the resistant organisms with the minimum inhibitory concentrations(MIC)at1.3,1.1and1.8mg/ml respectively;The antimi?crobial activities of alchoholic extracts of granatum against the above3bacterium were also high with the MIC at8.3,3.6and2.3mg/ml respectively.CONCLUSION:The alcoholic extracts of paeoniae radix and granatum have broad spectrum of an?timicrobial activity and which have antimicrobial effect to drug resistance bacteria.

Objective To investigate the utilization of enteral nutrition and parenteral nutrition in an inpatient pharmacy.Method The utilization of enteral nutrition and parenteral nutrition formulations in our inpatient pharmacy from 2008 to 2010 was analyzed in terms of brand,volume,and cost using the defined daily dose method.Results The amount of the brands of enteral nutrition formulations increased from 7 to 9 from 2008 to 2010,with the expenditure increased from RMB 426 489 yuan in 2008 to RMB 673 717 yuan in 2010.The amount of brands of parenteral nutrition formulations increased from 43 in 2008 to 50 in 2010,with the expenditure increased from RMB 8 913 103 yuan in 2008 to RMB 13 637 343 yuan in 2010.Conclusion Parenteral nutrition remains the dominant nutritional mode in our hospital and the utilization of enteral nutrition is still low.

Objective:To determine the effects of ginsenoside Rg1 on the expression levels of bone gla protein(BGP) and cytokine interleukin-6(IL-6) in the periodontium of experimental periodontitis rats.Methods:One hundred 160-200 g Wistar rats were randomly divied into ten groups.Group A used as control were killed at the beginning of the experiment.Group B-J were experimental periodontitis models.Group B-E received the therapy of ginsenoside Rg1 were killed at 1st,2nd,3rd and 4th weeks.Group F-J received no treatment as periodontitis control were killed at 0,1st,2nd,3rd and 4th weeks.The expression levels of BGP and IL-6 in the periodontium were measured by immunohistochemistry.Results:The expression levels of BGP in the group F were significantly lower than that in the group A,the expression levels of IL-6 in the group F were much higher than that in the group A(P

OBJECTIVE To investigate the tendency of bacterial distribution and resistance of hospitial infections,and to provide the reference for the clinical treatment and infection control in hospital.METHODS Bacteria isolated from patients in our hospital from Jan 2004 to Dec 2005 were identified by ATB expression,and bacterial susceptibility tests were performed on strains using Kirby-Bauer method.RESULTS A total of 3066 pathogens strains were isolated,among them 927 strains were Gram-positive cocci(30.2%).The most common pathogens of them were Staphylococcus.Meticillin resistant strains of Staphylococcus aureus and coagulase negative Staphylococcus(CNS) accounted for 69.0% and 77.6%,respectively.In our data,no vancomycin resistant S.aureus were isolated.There were 2134 strains of Gram-negative bacilli(69.6%),the most common pathogens of them were Pseudomonas aeruginosa,Escherichia coli,Klebsiella pneumoniae,Acinetobacter baumannii and Enterobacter cloacae.The ESBLs producing strains of E.coli and K.pneumoniae accounted for 30.1% and 40.1%,respectively.The highest susceptible to Gram-negative bacilli was carbapenem,then were cefoperazone/sulbactam,piperacillin /tazobactam and cefepime.Mainly pathogenic bacteria were multi-resistant to some antibiotics.CONCLUSIONS Drug resistance of the nosocomial infection bacteria is a serious problem.It's important and urgent to carry out surveillance of bacterial resistance for appropriate using of antibiotics and effective controlling nosocomial infections.

OBJECTIVE To investigate the tendency of bacterial distribution and drug resistance of pathogens in department of respiratory medicine,then instruct clinical application of antibiotics reasonably.METHODS By the retrospective methods,we analyzed the pathogens isolated from the sputa of the hospitalized patients with lower respiratory tract infection in the department of respiratory medicine from Jul 2007 to Jun 2009 and their drug resistance.RESULTS A total of 1508 strains of pathogens were isolated,Gram-negative bacilli were 47.3%,The most common pathogens of them were Pseudomonas aeruginosa(15.6%).The rate of the ESBLs-producing strains of Escherichia coli and Klebsiella pneumoniae were 41.0% and 35.8%,respectively.Fungi were 44.1%.And all the fungi were sentive to amphotericin B,fluconazole and ketoconazole.Gram-postive cocci accounted for 8.6%.The most common pathogen of Gram-postive cocci was Staphylococcus.Meticillin resistant strains of Staphylococcus aureus accounted for 62.4%.CONCLUSIONS The drug-resistance of pathogenic bacteria due to nosocomial infection and community-acquired infection in respiratory medical ward is increasing year-by-year.It is necessary to continuously monitor drug-resistance and rationally use antibiotics.