AIM: To explore a relative quantitative method by establishing HPLC digitized fingerprints of Fructus Gardeniae and taking the control medicinal material as a reference substance. METHODS: The chromatographic fingerprints were obtained by injecting the sample solution each time on a CenturySIL C_(18) BDS column (20 cm?4.6 mm,5 ?m) with the gradient elution solvent system composed of 1% acetate acid-water and 1% acetate acid-acetonitrile.The flow rate was 1.0 mL/min,the column temperature was maintained at(30?0.15) ℃ and the detection wavelength was set at 265 nm.The HPLC fingerprints of the control medicinal material for Fructus Gardeniae were obtained under different injection volumes and calculated by "the digitized evaluation system of the traditional Chinese medicine fingerprint with the super-information characteristics".Equivalent regression curves were obained by making linear regression between the fingerprint peak areas and apparent injection amounts.(RESULTS:) 35 co-possessing peaks were selected as the fingerprint peaks of Fructus Gardeniae by taking Geniposide peak as the reference peak.Both the equivalent relation between each of the ten places and control medicinal material of Fructus Gardeniae and the equivalent relation between each fingerprint peak of different places and that of Fructus Gardeniae control medicinal material were calculated by equivalent regression curves. CONCLUSION: This method can be useful in the quality control of Fructus Gardeniae.It is also of importantance in deeply studying the relative amounts of single fingerprint peak,and it provides reference value in extending the quantitative evaluation methods of TCM.

AIM:To establish a digitized HPLC fingerprint of Compound Danshen Dropping PilLs (CDDP). METHODS:The RP-HPLC condition was optimized by the chromatographic fingerprint relative index (Fr). The chromatographic fingerprints were characteristically digitized and evaluated by the software of the digitized evaluation system of Traditional Chinese Medicine (TCM) with the super information characteristics. RESULTS:15 co-possessing peaks were selected as the fingerprint peaks of CDDP by taking protocatechualdehyde peak as the referential peak to establish the digitized fingerprint and the important digitized information for its quality control was obtained. The stabilities among different lots of samples were evaluated by the dual qualitative and dual quantitative similarity method. CONCLUSION:The fingerprints can perfectly reflect the authentical quality of CDDP.

AIM: A novel method for the overall quality control of Herba Artemisiae scopareiae has been established by HPLC fingerprint. METHODS: The chromatographic fingerprints were obtained by injecting ~5 ?L of the sample solution each time on a Kromasil ODS column (250 mm?~4.6 mm ,~5 ?m ) with the gradient elution solvent system composed of 1%acetate acid-water-methanol. The flow rate was 1 mL/min, the column temperature was maintained at ~30 ℃ and the detection wavelength was set at ~326 nm . RESULTS:22 co-possessing peaks were selected as the fingerprint peaks of Herba Artemisiae scopareiae and the similarities between the chromatographic fingerprints of the herbal drugs were calculated taking chlorogenic acid peak as the reference peak. The chromatographic fingerprints also were evaluated by the chromatographic fingerprint index (F), the relative index (Fr) and the fingerprint components apparent prercentage contents (P%). CONCLUSION: This method with good precision and reproducibility can be useful in the quality control of Herba Artemisiae scopareiae.

Objective To establish the quality control of Yinhuang tablet (YHT) by using sever ingredients determina-tion and tri-wavelength fingerprint analysis technologies. Methods The chromatographic fingerprints were developed by using Agilent Poroshell 120 SB C18(4. 6 mm×150 mm, 2. 7 μm) as the separation column and 0. 2% H3 PO4-methanol as the mobile phase. The flow rate was 0. 5 mL·min-1 , the UV absorbance was monitored at 214, 278 and 326 nm by injecting 5 μL of the sample solution and the column temperature was maintained at (35. 00±0. 15) ℃ . The chromatographic fingerprints were charac-teristically evaluated for quality ranking of 13 batches of YHT by the 4. 0 software of the digitized evaluation system of traditional chinese medicine fingerprint with the super information characteristics. Seven marker compounds including chlorogenic acid, caf-feic acid, baicalin, wogonoside, baicalein, wogonin and scutellarin were simultaneously determined to compare the variation a-mong five different manufacturers. Results The tri-wavelength fingerprint high performance liquid chromatography method was established. A total of 28(214 nm),29(278 nm)and 26(326 nm)common peaks were identified at respective wavelengths, using baicalin (BCL) as the referential peak. The software was applied to evaluate the YHT-HPLC fingerprints and identify the quality of 13 batches of YHT by systematically quantified fingerprint method (SQFM). The results showed that the content from the dif-ferent manufacturers varied greatly. Conclusion The quality of YHT varies between different makers, and is necessary to es-tablish the criterion for quality control. This method can provide a new reference for the quality control of YHT.

AIM: To simutaneously determinate the four components of dipyridamole, quercetin,kaempferol and isorhamnetin in Ginkgo-Leaf-Extract Dipyridamole Injection by the reversed-phase high performance liquid chromatographic (RP-HPLC). METHODS: Ginkgo-Leaf-Extract Dipyridamole Injection was determined by RP-HPLC on Scienhome Kromasil C_ 18 (250 mm? 4.6 mm, 5 ?m) column. The mobile phase consisted of methanol and 0.4% phosphoric acid solution (55∶ 45, v/v), the flow rate of 0.80 mL/min and UV detection wavelength at 360 nm were set to determine the contents of the four components. RESULTS: There were good linear relationships between peak area and concentration of the four components, and the calibration curves were linear in the ranges of 21.7 -261.0 ?g/mL for dipyridamole, 8.0 -96.0 ?g/mL for quercetin, 7.75 -93.0 ?g/mL for kaempferol, 5.75 -69.0 ?g/mL for isorhamnetin. The precisions for dipyridamole, quercetin, kaempferol and isorhamnetin were 1.16% , 1.35% , 1.21% and 1.58% , respectively. The average recoveries were 100.3% , 100.0% , 99.86% and 99.80% , respectively. CONCLUSION: The four components of the dipyridamole, quercetin, kaempferol and isorhamnetin in Ginkgo-Left-Extract Dipyridamole Injection are determined at the same time, and the method is simple, sensitive and accurate.

Objective To study how to use the machine of B.BRAUN's Diapact continuous renal replacement therapy (CRRT) to achieve multiple models of plasma treatment by analyzing the characteristics of the machine.Methods Based on the machine of B.BRAUN's Diapact CRRT,assisted by extra single pump,the pipeline connection of the machine was reformed and the appropriate parameter was reset.Results After the reconstruction,the new equipment could succeed in achieving special plasma treatment such as double filtration plasmpheresis (DFPP)and plasma adsorption (PA).Conclusions The reconstructed equipment can attain special plasma treatment.Compared with plasma exchange (PE),the equipment has some characteristics such as few syndromes and without external plasma.According to its safety and stability,this new method is suitable for clinical application.

AIM:To develop a new method to determine cucurbitacin B in Pedicellus Melo by capillary zone(electrophoresis(CZE).) METHODS:All the samples were analysed on a fused silica capillary(75 cm?75 ?m I.D.) by using 50 mmol/L sodium borate solution(containing 5% acetonitrile) as the background electrolyte with the running voltage at 12.0 kV and detection wavelength of 265 nm.And clorprenaline hydrochloride was applied as the internal standard. RESULTS:The results showed a good linear correlation between relative peak area of cucurbitacin B(y) and its concentration(x) over the range of 0.15～1.2 mg/mL.Regression equation was y=(0.003 4x)+0.058,r=0.999 7,and the average recovery for baicalin was 100.2% with the RSD at(2.11%). CONCLUSION:The method is rapid,simple,reproducible and produces low pollution.It serves as a novel way to determine cucurbitacin B.

AIM:To establish a HPLC diagitized fingerprint for Ixeris sonchifolia Hance Injection to serve as a digitized method criteria for its overall quality control. METHODS: The chromatographic fingerprints were obtained by injecting 20 ?L of the sample solution each time on a Century SIL BDS column (250 mm?4.6 mm, 5 ?m) with the gradient elution solvent system composed of 1% acetic acid water and 1% acetic acid acetonitrile. The flow rate was 1 mL/min, the column temperature was maintained at (30?0.15)℃ and the detection wavelength was set at 265 nm. The chromatographic fingerprints were charateristically digitized and evaluated by the software of the digitized evaluation system of traditional Chinese medicine fingerprint with the super information characteristics to give the 42 parameters such as chromatographic fingerprint index (F), chromatographic fingerprint resolution index (RF), which could thoroughly disclose the hidden information in the fingerprints. Meanwhile, the apparent molecular weight, the peak position, the elution fore index and the pseudo peak area were marked in terms of the principle of QSPR when the two peaks were selected as the reference peaks. RESULTS: 21 co-possessing peaks were selected as the fingerprint peaks of Ixeris sonchifolia Hance Injection by taking caffeic acid peak as the reference peak to establish the digitized fingerprint and obtain the important digital information about its quanlity control. The stabilities among different batches of samples were evaluated by the dual qualitative and dual quantitative similarity method. CONCLUSION: This new digitized fingerprint method with good precision and reproducibility can be perfectly applied to the quality control over Ixeris sonchifolia Hance Injection. Digitized fingerprint obtained from the dual qualitative and dual quantitative similarity method maybe extend further to assess and control the quanlity of TCM.

Objective To explore the expression level of calmodulin (CaM) in non-small cell lung carcinoma (NSCLC) and its clinical significance. Methods The expression levels of CaM and CaM mRNA were detected in 64 human NSCLC samples by immunohistochemistry and in situ hybridization, respectively. Results The positive rates of CaM mRNA and CaM protein were 60.4% and 65.6%, respectively. The positive rates of CaM mRNA in low-differentiated samples were significantly higher (70.0%) than those in moderate- to well-differentiated samples (45.3%). The expression rates of CaM in Ⅲ-Ⅳ stages (81.8%) were significantly higher than those inⅠ-Ⅱstages (51.6%) (P

Objective To explore the relationship of the expression levels of laminin (LN) and calmodulin (CaM) in non-small cell lung carcinoma (NSCLC) with its metastasis and prognosis. Methods The expressions of CaM and LN were detected in 64 human NSCLC samples by immunohistochemistry. Results The expressions of LN and CaM were closely associated with the differentiation and the grades of NSCLC as well as the lymph node metastasis. The positive rates of LN in adenocarcinoma and squamous cell carcinoma were 45.1% and 69.7 (P0.05), respectively. The positive rate of LN protein in low-differentiated samples (36.6%) was significantly lower than that in moderate- to well-differentiated samples (61.7%), but the positive rates of CaM were 73.3% in low-differentiated samples and 41.1% in moderate- to well-differentiated samples (P