Objective To observe the influences of VEGF transfection on the proliferation and ultrastructure of hBMSCs. Methods Three groups were enrolled in the experiment: group of Ad-VEGF transfection, group of empty Ad transfection, and the control group. Cells were observed continually by inverted phase contrast microscope, and stained by HE. Proliferation of cells was tested by MTT and by flow cytometry analysis. Results Histological observation and observation through inverted phase contrast microscope showed that the cells were of the similar morphology in the 3 groups. As time elapsed, the amount of cells increased, but still no obvious differences were found in optical density (OD) value of hBMSCs.Groups B, C, A had a similar percentage of DNA G1 period and a similar proliferation index (PrI) ( P >0. 05). Conclusion Transfeetion of VEGF has no obvious influence on the prohferation and morphologyof hBMSCs in vitro.

Objective To discuss the diagnosis and operative treatment of the complex acetabular fractures of Letournel classification. Methods On the basis of the three- dimensional computed tomography, 75 cases of complex acetabular fractures were diagnosed and classified according to Letournel classification. They were treated through the anterior, posterior, combined anterior- posterior and the improved iliofemoral approaches. All the fractures were fixed with screws and AO reconstruction plates. Results All the cases were followed up for 6 to 96 months, with an average time of 46 months. They were evaluated according to D' Aubigne and Pestel criteria for joint functions and Epstein criteria for radiographic manifestation. 34 cases of the series were rated as excellent (45.23% ), 28 case as fine (37.33% ), 8 cases as fair (10.67% ) and 5 cases as poor (6.67% ). Conclusion Enough image data, simulation in vitro on a pelvic specimen, maximal anatomical reduction and appropriate approach are the basis for satisfactory outcomes.

Objective To discuss a new method of BMSCs culture which can reduce the lead-in of extraneous antigenic substances in the process of cell culture, and can thus better meet the demand on the quantity and biological characteristics of BMSCs in the clinical use of tissue engineering bone. Methods BMSCs from a human adult in vitro were induced by using autologous platelet-rich plasma instead of animal serum in high-carbohydrate DMEM (Dulbecco's minimum essential medium). After the cultured BMSCs were amplified rapidly by Rotary Cell Culture System, their growth status was observed and their biological characteristics were detected. Results The cells were in good growth status and presented fine biological characteristics. Their growth speed in Rotary Cell Culture System was observed to be evidently faster than that by conventional methods. Conclusions Rotary Cell Culture System by autologous platelet-rich plasma instead of animal serum is a good culture method to induce human BMSCs in vitro. The quantity and biological characteristics of BMSCs cultured in this way can meet the demand in clinical use.

Objective To explore relationship between the plasma calcitonin-gene related peptide(CGRP) level and brain damage in neonatal asphyxia. Methods Dynamic variation of plasma CGRP level was monitored in 62 asphyxiated newborn infants and 21 normal infants by radioimmunoassay, and the relation between the brain damage and CGRP in neonatal asphyxia was analyzed. Results Plasma CGRP level markedly elevated at acute stage of neonatal asphyxia(P

AIM:To investigate whether minocycline postconditioning protects rat myocardium from ischemia-reperfusion ( I/R ) injury through attenuating poly ( ADP-ribose ) polymerase-1 ( PARP-1 ) excessive activation. METHODS:The left anterior descending coronary artery was ligated for 45 min and then reopened for 2 h to establish the rat model of myocardial ischemia-reperfusion injury.The male Wistar rats ( n =90 ) were randomly divided into sham group, I/R group, low-and high-dose minocycline groups, and 3-aminobenzamide (3-AB, PARP inhibitor) group.The myocardial infarct size was measured by Evans blue and 2,3,5-triphenyltetrazolium chloride ( TTC) staining.The morpho-logical changes of the myocardium were observed with HE staining.The cardiomyocyte apoptosis was detected using in situ TDT-mediated dUTP nick end labeling ( TUNEL) .The level of tumor necrosis factorα( TNF-α) and interleukin 1β( IL-1β) in the serum were measured by ELISA.The content of poly( ADP-ribose) ( PAR) in the reperfused myocardium and peripheral leukocytes were detected by Western blot.RESULTS: Compared with sham group, PAR expression, TNF-αcontent and IL-1βconcentration increased in all other groups.Compared with I/R group, treatment with low and high doses of minocycline and 3-AB significantly reduced the infarct size and myocardial apoptosis.PAR expression, TNF-αcontent and IL-1βconcentration in low-and high-dose minocycline groups and 3-AB group all decreased.No significant difference of the above parameters between high-dose minocycline group and 3-AB group was observed.CONCLUSION: Minocy-cline postconditioning may attenuate myocardial ischemia-reperfusion injury by depressing the activation of PARP-1 in car-diomyocytes and peripheral leukocytes in rats.

Limb lengthening has been applied to deal with inequality of lower limb for a long time. In some special cases femoral lengthening can be chosen for the treatment, though this technique is more difficult than tibial lengthening. We have reviewed in this paper the indications, different methods, newest devices and skills, prevention and cure of complications in femoral lengthening. Because of the high incidence of complications due to this operation, doctors should be very cautious when they determine the cases for the operation.

To prepare an experimental caprine model of tibia bone diaphyseal defect used in tissue engineering and defect repair with the tissue engineering method, 27 Chinese caprines were divided into 3 groups: blank, control, and test. The 20mm left tibia diaphyseal defect of each caprine was made and fixed with plates. The blank group was not filled with coral hydroxyapatite (CHAP) or bone marrow stroma cell (BMSc). The control group and the experimental group were filled with CHAP and CHAP/BMSc respectively. The results of three groups were evaluated by X ray examination, optical density index of X ray film and histology 4, 8, 12 weeks after operation. The biomechanical characteristics of the specimens of the CHAP group and the CHAP/BMSc group were tested by three point bending test 12 weeks after operation.The results showed that the optical density indices of X ray film of the blank group were not significantly different 4, 8, 12 weeks after operation and indices of the CHAP group were significantly smaller than those of the CHAP/BMSc group ( P

Objective: To evaluate the clinical effectiveness and safety of early total enteral nutrition (TEN) in severely burned patients. Methods: Forty one burned patients with total burn surface area over 30% were randomly assigned to TEN group (n=21) and CONT group (n=20). The two nutritional support protocols were similar in calorie and nitrogen intake within 7 d of treatment. Serum levels of visceral proteins and TNF-? were measured. Prognostic inflammatory and nutritional index (PINI) was postulated from formula. Results: Contrasting to a significant reduction (from 96.0?31.8mg/L to 69.4?17.3 mg/L, P

BACKGROUND: The main aspect of the study in the bone histological engineering is how to maintain and improve theosteogenesis of the osteoblasts in vivo and in vitro. The gene transference may provide a new effective method to deal with theproblem.OBJECTIVE: To discuss the effect of the reverse transcription virus mediated human bone morphogenetic protein7(hBMP-7) gene transfection on the proliferation and osteogenetic differentiation of the bone marrow mesenehymal stemcells (BMSCs) of the rabbits.DESIGN:Cells taken as the study object, grouping control, repeat observation andmeasurement.SETTING: Traumatological and othopaedic lab of a medical university hospital.PARTICIPANTS: The study wascompleted in the Traumatological and Othopaedic Lab in the Affiliated Nanfang Hospital of the Southern Medical University from July 2001 to July 2003. Four New Zealand rabbits,whose weights varied from 1.0 to 1.5 kg, were provided without sexlimit by the Animal Experiment Center of the First Military Medical University of Chinese PLA.METHODS:The reverse transcription virus carriersof the hBMP-7 were constructed,and then the BMSCs were transfected by the virus containing target genes. The expression of the hBMP-7 protein was detected with the immunohistochemical method. The cell proliferation, cycle and ALP synthesis were respectively detected with the MTT method,flow cytometer and NPP method.MAIN OUTCOME MEASURES: Primary results: ① the detection results of the cell proliferation. ② the detection results of the ALP.Secondary results: ① the expression of the hBMP-7 protein in the transfected BMSCs. ② the detection results of the cell cycle.RESULTS: After the BMP-7 gene transfection, there was hBMP-7 positive expression in the BMSCs of the rabbits,using the immunohistochemical detection. There was no significant change in the BMSCs proliferation of the rabbits after the hBMP-7 gene transfection ( P ＞ 0.05). Compared with the ALP synthesis of the transfected BMSCs(294. 592 ± 86. 567) nkat/L, there was significant difference in the ALP synthesis of the empty carrier transfected BMSCs(155. 231 ±86.567) nkat/L and the un-transfected BMSCs (160. 866 ±91. 585)nkat/L( F =5. 660, P ＜ 0. 05).CONCLUSION: After the BMP-7 gene transfection, the BMSCs can synthesize and express the extragenous BMP-7. The hBMP gene transfection can promote the differentiation of the BMSCs cultured in vitro into the osteoblasts and can be used as the seed cells in the construction of the histological en gineering bone tissues and in further application.