Objective To study the stability of the plasma glucose in sample tubes containing different glycolysis inhibitors and anticoagulants at room temperature for up to 24 h.Methods Venous blood was collected from 20 volunteers.Each donor blood sample was divided into six tubes containing different glycolysis inhibitors and anticoagulants and kept at room temperature.Plasma glucose was measured at 0,2,4,8 and 24 h after collection.The difference of plasma glucose levels for each sample tube was com-pared with different placed time and the difference of plasma glucose levels for the same placed time was compared with different sample tubes.Results The differences of the plasma glucose results of 2 hours after sampling and of instant detecting are statisti-cally significant for each sample tube(P<0.05).The differences of the plasma glucose levels among the three sample tubes contai-ning sodium fluoride are not statistically significant at the same placed time(P>0.05).The differences of the plasma glucose results of 2,4,8,24 h after sampling for each sample tube are not statistically significant(P>0.05).The differences of the plasma glucose results for each sample tube containing sodium iodoacetate are statistically significant at different placed times(P< 0.05).The differences of plasma glucose levels of 0,2,4 hours after sampling for the three sample tubes are no statistically significant(P>0.05)and the differences of plasma glucose levels of 8,24 hours after sampling for the three sample tubes are statistically signifi-cant(P<0.05).The differences of plasma glucose levels for the two glycolytic inhibitors are statistically significant at 4,8,24 hours after sampling(P<0.01)except 2 hours after sampling(P>0.05).Conclusion Tubes containing NaF are more suitable for detec-ting venous plasma glucose within 24 h at room temperature.The type of anticoagulant has no obvious correlation with the ability of glycolytic inhibition.