1.Integrative construction of animal health systems and public health
Chinese Journal of Veterinary Science 2009;29(7):944-948
Animal health systems initially established in Australia,Canada and other countries,played a very important role in prevention and control of animal diseases.Based on comparing the animal health systems of Australia with the veterinary medical system in China,some strategies was obtained for establishing a more reasonable official veterinary system and animal health system in China,thus speeding up the integration process of veterinary and human medical public health system.
2.Study on a Grey Model for Evaluation of Anopheles minimus Density
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(04):-
wet season temperature_max. The An. minimus density evaluation model was derived as follows: y=0^0578 e~0^0780(8X_10′+7X_12′+6X_11′+5X_15′+4X_9′+3X_4′+2X_8′+1X_7′) The correct rate of evaluation by the model was 92^0%. e_0^5=18%, with an average relative error of 21%. Conclusion A quasi-evaluation on the An. minimus density can be made by applying the grey model.
3.EFFECT OF ZINC SULFATE AND ZINC METHIONINE ON GROWTH AND THEIR MECHANISM IN MICE
Zepeng YU ; Guowei LE ; Yonghui SHI
Acta Nutrimenta Sinica 2004;0(06):-
Objective: To investigate the effect of zinc sulfate and zinc methionine on growth and their possible regulating mechanism in mice. Method: Ninety male KM mice were randomly divided into three groups. The control group was fed on basal diet containing zinc of 11. 67 mg/kg 10d. The ZnSO4 group and Zn-Met group were fed on the diets supplemented with ZnSO4 or Zn-Met at 30 mg/kg(on the basis of Zn) for 10 d. Initial and final body weight,serum zinc concentration, growth hormone (GH),the levels of growth hormone receptor (GHR) and insulin like growth factor 1 (IGF-1) mRNA were determined. Results: Both ZnSO4 and Zn-Met enhanced body weight and serum zinc concentration of mice,Zn-Met more effectively than ZnSO4 for body weight . Both forms of zinc had no effect on GH and the expression of GHR mRNA , but both up-regulated the expression of IGF-1 mRNA. As compared to ZnSO4, Zn-Met enhanced the level of IGF-1 mRNA significantly. Conclusion: Both ZnSO4 and Zn-Met had no effect on GH and the expression of GHR Mrna,but enhanced the expression of IGF-1 mRNA. Zn-Met enhanced the body weight gain and up-regulated IGF-1 mRNA expression more effectively than ZnSO4.
4.Evaluating the physical activity status of 259 advanced aged people
Guilan YU ; Huidi CUI ; Guowei HU
Chinese Journal of Geriatrics 2003;0(07):-
Objective To evaluate the physical activity status of the advanced aged adults and determine the influence of long-term physical activities on the physical function and health of the advanced aged adults. Methods A survey was carried out from March, 2004 to September, 2004, among those advanced aged intellectuals or cadres taken care chronically by the Patient Clinic for Senior Cadre in PUMC Hospital. Physical activity was assessed using the modified leisure-time physical activity questionnaire. Results There were 259 participants, aged from 80 to 94 (83.92?3.41)years. 85.3%(221/259 cases)took physical activities almost every day for more than eleven years, and 21.7% of them (48/221 case)even stuck to it from their youth. Among these 221 participants, 34.4% took≥ 60 min per day of physical activities,and 51.0% ≥30 min per day. The group that took ≥ 60 min per day held a lower proportion than the group that took ≥ 30 min per day to suffer from agrypnia, debilitation, somasthenia and anorexy (by 20.3%, 24.0%, 21.7% and 13.1%,respectively(P
5.Study on influencing factors of nursing unit workload using Delphi method
Yuxia MA ; Juxia ZHANG ; Lin HAN ; Guowei YU
Chinese Journal of Practical Nursing 2013;(2):6-9
Objective In order to measure the nursing unit workload scientifically,we used the Delphi method to investigate the influencing factors of nursing unit workload.Methods After consulting literature and discussing with experts,alternative indicators were designed,then two rounds of consultations were made with experts by using the Delphi method.Results The questionnaires revealed that the valid recovery rate,after two rounds of consultations,were 95% and 100% respectively,which reflected the positivity of the experts which participated in this study.The expert authority coefficient was 0.896,which indicated the high reliability of the consulting.The coordination indexes of expert opinions were 0.467 and 0.239 respectively,which showed the expert advices had good coordination and the results had high reliability.Conclusions The influencing factors of nursing unit workload determined by Delphi method have high reliability,which is significant for the scientific measuring of the nursing unit workload.
6.Correlation between total bile acid/phospholipid ratio and relative warm ischemia injury to the biliary tract in rat liver transplantation
Xuanlei REN ; Hongfeng ZHAO ; Guowei ZHANG ; Yu WANG ; Jie ZHOU
Chinese Journal of Tissue Engineering Research 2010;14(5):818-823
BACKGROUND: Studieshave shown that long time of warm ischemia or cold preservation would injury the biliary tract in liver transplantation. However, whether relative warm ischemia (RWI) of biliary tract would result in bile component changes is unclearly. OBJECTIVE: To establish auto-liver transplantation bile ducts RWI models, observe the effects of RWI on the bile salts and phospholipid concentration secreted by the donor liver, and to study the correlation between the total bile salt/phosphoUpid ratio (TBA, PL ratio) and billary tract injury. METHODS: A total of 32 SD rats were selected for auto-liver transplantation models with bile ducts RWI, and the rats were randomly divided into 4 groups (n=8). In Group Ⅰ (sham operation group), rats only received liver dissociation without any cold reperfusion. The RWI time of Group Ⅱ,Ⅲ, and Ⅳ were 0 minutes, 30 minutes and 60 minutes, respectively. The concentration of TBA in bile was measured with enzymatic cycling assay, andPL with enzymic colorimetric. Pathological observation with light microscope and ultrastructural observation with transmission electron microscope were performed on the hilar bile duct. The endothelial cell apoptosis was detected with TUNEL assay. The correlation between TBA, PL ratio and biliary injury was analyzed. RESULTS AND CONCLUSION: One rat died, the other 31 rats were included in the final analysis. RWI could change the composition of bile secreted by donor liver, raise the TBA/PL ratio, and increase the bile toxicity. These changes had a positive correlation to RWI time, and the changes were obviously with time prolonged. In addition, the changes are closely related to the biliary tract injury. This study shows an important mechanism of the biliary tract injury caused by RWI-injury.
7.Expression of MAGE-A3 Gene and MDR1 Gene in Non M3-subtype Acute Leukemia and their correlations to dinical treatment efficacy
Guowei LI ; Dongning WANG ; Hongmei WU ; Xiang YU ; Xianyin XU
Journal of Chinese Physician 2009;11(4):474-476
Objective To evaluate the clinical value of co-observation of the expression of MAGE-A3 gene and MDR1 gene on esti-mating the curative effect in non M3-subtype acute leukemia. Methods Expressions of MAGE-A3 and MDRI were measured in 77 patients with non M3-subtype acute leukemia by RT-PCR method. Clinical observation was done to estimate the relationship between the genes with curative effect in non M3-subtype acute leukemia. Results Expression of MAGE-A3 and MDRI gene were 50. 6% and 23. 3% in non M3-subtype acute leukemia patients. Positive expression of MDRI in MAGE-A3-positive and negative patients were 46. 2% and 13. 2% (P < 0.01). The complete remission rate in MAGE-A3 negative and positive patients were 86. 8% and 64. I% (P <0. 05). Complete remission (CR) rate in MDR1 negative and positive patients was 83.3% and 56. 5% (P <0. 05). Complete remission rate were 87.9% and 55.6% in beth negative and both positive expression of MAGE-A3 and MDR1 (P < 0. 05). Conclusion The patients of positive expression of MAGE-A3 in non M3-subtype AL had higher expression of MDR1. The patients with negative expression of beth MAGE-A3 and MDR1 had higher CR rate than that in both positive patients. These researches indicated that eo-observatian of the expression of MAGE-A3 and MDR1 can predict the curative effect in non M3-subtype AL.
8.The Study and Value of Radiologic Anatomy of Mammary Duct
Wu YU ; Guowei YIN ; Longjiao ZHANG ; Xiaoyu FAN ; Xuemei WU
Journal of Practical Radiology 1992;0(11):-
Objective:To improve the radiologic diagnostic and treatable rate of mannmmary ductal diseases.Methods:We retrospectively analyzed the galactographic materials of 369 cases.Results:The mammary ducts were classified into 4 types according to their morpholgy,including general fruticoses,multiple fruticoses,less fruticoses and single fruticoses.In this group,the multiple and less fruticoses were the most common,the general and single fruticoses were the less.Conclusion:Understanding radiologic anatomy of mammary duct is very valuable in the diagnosis and treatment of mammary ductal diseases. [
9.Study on high incidence of hepatitis C and its epidemiological features in Jianping county, Liaoning Province
Zongfen LI ; Yiping FENG ; Lianzheng YU ; Li LIU ; Liya YU ; Liying XING ; Lixia HE ; Guowei PAN
Chinese Journal of Infectious Diseases 2009;27(12):746-748
Objective To investigate the significantly elevated incidence of hepatitis C and mortality of cirrhosis and hepatocellular carcinoma (HCC) in Jianping county, and to explore the epidemiological features. Methods The data from database of death registry and infectious disease surveillance in Jianping county, Liaoning Province were analyzed. The distributions of incidence of hepatitis B and hepatitis C, mortality of cirrhosis and HCC in 23 villages and towns were investigated.Spearman's correlation was used to explore the correlations between hepatitis, cirrhosis and HCC.Results The standardized mortality of HCC in males and females in Jianping county were 77. 6/10~5and 22. 0/10~5, respectively, which were 2. 0 and 1. 7 times, respectively of the average levels of Liaoning rural areas. The incidence of hepatitis C was 58. 0/10~5 , which was 9. 5 times of the averagelevel of Liaoning Province. There were positive correlations between incidence of hepatitis C and mortality of cirrhosis (r=0. 495, P = 0. 008), and mortality of cirrhosis and HCC (r=0. 646, P<0.01). Conclusions The incidence of hepatitis C and mortality of cirrhosis and HCC in Jianping county are significantly higher than the average levels of Liaoning Province. Further investigations of the suspected causes are needed.
10.Study of nuclear proteomics for splenic lymphocytes of lupus mice affected by recipe of nourishing yin and clearing heat
Wei WU ; Ruiqiang FAN ; Jiekai YU ; Xian ZHANG ; Guowei XUAN ; Jianhong CHEN
China Journal of Traditional Chinese Medicine and Pharmacy 2006;0(09):-
Objective:To probe e ect of a Chinese compound recipe of nourishing yin and clearing heat and prednisone on nuclear protein of splenic lymphocytes in mice.Methods:MRL/LPR female lupus mice were divided into four groups respectively treated with isotonic Na chloride,traditional Chinese medicine,Western medicine and integrated Chinese and Western medicine,then nucleoproteins of splenic lymphocytes were extracted and detected by SELDI-TOF-MS method.Result:There were some proteins in control group which aggravated autoimmune course,and some proteins in Western medicine group which rivalried immunological activity;integrated Chinese and Western medicine and Western medicine showed similarly e ect on nucleoprotein of splenic lymphocytes.Conclusion:The recipe of nourishing yin and clearing heat and prednisone can treat the disease by uencing in the di erentiation of splenic lymphocytes and the expression of proteins.