1.Clinical value of intrafascial hysterectomy with intact ascending uterine artery
Chinese Journal of Postgraduates of Medicine 2011;34(33):22-25
ObjectiveTo investigate the clinical value of intrafascial hysterectomy with intact ascending uterine artery.MethodsA total of 78 cases of simple hysterectomy patients were divided into group A (underwent intrafascial hysterectomy with intact ascending uterine artery) and group B(underwent modified intrafascial hysterectomy) with 39 cases each by random digits table.The operation time,the amount of hemorrhage,the postoperative complications,the ovarian function and the quality of sexuality were studied and compared.ResultsThree cases were lost in group B.There were no statistical differences in operation time,the amount of hemorrhage,postoperative hemorrhage,postoperative pyrexia,the quality of sexuality between two groups(P >0.05).There were significant differences in follicule-stimulating hormone and estradiol level in group B compared with those before operation (P< 0.05).There was significant difference in climacteric symptom between two groups [ 5.13% (2/39) vs.22.22% (8/36) ](P < 0.01 ).The ascending uterine arteries and the ovarian branch of both sides were showed 100% in group A.ConclusionsIntrafascial hysterectomy with intact ascending uterine artery has less influences on the blood flow of the ascending uterine arteries and the ovarian branch,there is slight influence on ovarian function and has no obvious influence on the quality of sexuality.The forward clinical effects should be studied further.
2.The Study and Value of Radiologic Anatomy of Mammary Duct
Wu YU ; Guowei YIN ; Longjiao ZHANG ; Xiaoyu FAN ; Xuemei WU
Journal of Practical Radiology 1992;0(11):-
Objective:To improve the radiologic diagnostic and treatable rate of mannmmary ductal diseases.Methods:We retrospectively analyzed the galactographic materials of 369 cases.Results:The mammary ducts were classified into 4 types according to their morpholgy,including general fruticoses,multiple fruticoses,less fruticoses and single fruticoses.In this group,the multiple and less fruticoses were the most common,the general and single fruticoses were the less.Conclusion:Understanding radiologic anatomy of mammary duct is very valuable in the diagnosis and treatment of mammary ductal diseases. [
3.Effect of lipoic acid on redox regulation,digestive and absorptive function in intestine of mice fed high fat diet
Wu LI ; Yonghui SHI ; Ruili YANG ; Peng YUE ; Guowei LE
Chinese Journal of Pathophysiology 1986;0(03):-
AIM:To elucidate the molecular mechanisms of lipoic acid (LA) on redox regulation and digestive function in intestine of C57BL/6 mice fed high fat diet (HFD).METHODS:C57BL/6 mice were randomly assigned to one of three groups (n=8). The control group consumed an ordinary diet. The other two experimental groups were fed with a high fat diet,high fat plus 0.1% LA. After 6 weeks,the activities of digestive enzymes were examined. In order to evaluate the antioxidant status of the mice,superoxide dismutase (SOD),malondialdehyde (MDA),total antioxidant capacity (TAC) and reactive oxygen species (ROS) in intestinal homogenate were measured. To investigate the molecular mechanisms underlying the effects of LA,the gene expression profiles in intestine were examined using the GeneChip microarray system.RESULTS:A depressed antioxidant defense system,accompanied by digestive and absorptive function impairment,was observed in HFD-fed mice. These changes were partially restored in the LA-treated group. DNA microarray analysis of intestine showed that LA ingestion up-regulated the expression of genes related to free-radical scavenger enzymes,digestive enzymes and transporters.CONCLUSION:Treatment with LA improves redox homeostasis and the function of intestine in mice fed HFD. The mechanism may involve preventing oxidative stress by scavenging ROS directly and increasing those of free-radical scavenger enzymes gene expression indirectly.
4.Expression of MAGE-A3 Gene and MDR1 Gene in Non M3-subtype Acute Leukemia and their correlations to dinical treatment efficacy
Guowei LI ; Dongning WANG ; Hongmei WU ; Xiang YU ; Xianyin XU
Journal of Chinese Physician 2009;11(4):474-476
Objective To evaluate the clinical value of co-observation of the expression of MAGE-A3 gene and MDR1 gene on esti-mating the curative effect in non M3-subtype acute leukemia. Methods Expressions of MAGE-A3 and MDRI were measured in 77 patients with non M3-subtype acute leukemia by RT-PCR method. Clinical observation was done to estimate the relationship between the genes with curative effect in non M3-subtype acute leukemia. Results Expression of MAGE-A3 and MDRI gene were 50. 6% and 23. 3% in non M3-subtype acute leukemia patients. Positive expression of MDRI in MAGE-A3-positive and negative patients were 46. 2% and 13. 2% (P < 0.01). The complete remission rate in MAGE-A3 negative and positive patients were 86. 8% and 64. I% (P <0. 05). Complete remission (CR) rate in MDR1 negative and positive patients was 83.3% and 56. 5% (P <0. 05). Complete remission rate were 87.9% and 55.6% in beth negative and both positive expression of MAGE-A3 and MDR1 (P < 0. 05). Conclusion The patients of positive expression of MAGE-A3 in non M3-subtype AL had higher expression of MDR1. The patients with negative expression of beth MAGE-A3 and MDR1 had higher CR rate than that in both positive patients. These researches indicated that eo-observatian of the expression of MAGE-A3 and MDR1 can predict the curative effect in non M3-subtype AL.
5.Modified Nuss procedure with thoracoscope for repairing pectins excavatum in children:a report of 49 cases
Jie DU ; Xingti HU ; Qifeng ZHAO ; Guowei WU
Chinese Journal of Postgraduates of Medicine 2009;32(2):23-25
Objective To summarize the experience of modified Nuss procedure with thoracoscope for the correction of pectus excavatum in children. Methods Forty-nine pediatric pectus excavatum were treated by modified Nuss procedure with thoracoscope from August 2006 to July 2008, the age ranged from 3 to 18 years, 36 patients were symmetric pectus excavatum and 13 patients were asymmetric pectus excavatum. Results The operations in all patients were successful. Only 1 case used two bars,other 48 cases used one bar. There were no major complications such as cardiac perforation occurred during the operation. The earlier complication was pneumatothorax in 1 case, and the average blood loss was less than 10 ml. The postoperative hospital length of stay was 6-8 days. All patients had a satisfied deformity correction and no needs of transfusion. Therapeutic results evaluation showed excellent in 43 patients and good in 6 patients. All patients were followed up in 0.5-23.0 months without bars displacement, 1 ease had chronic intermittent pain for 2 months. The bar had been removed in 1 case after operation 23 months who still kept in excellent result. Conclusions Modified Nuss procedure is easy to performed with minimal invasion and satisfied deformity correction. Thoracoscopic visualization facilitates the safety of this technique.
6.Clinical Observations on Heat-sensitivePoint Yaoyangguan Moxibustion for Treatment of Sciatica
Guowei CAI ; Jing LI ; Pengfei LI ; Man WU
Shanghai Journal of Acupuncture and Moxibustion 2014;(12):1141-1143
ObjectiveTo investigate the clinical efficacy of heat-sensitivepoint Yaoyangguan(GV3)moxibustion for sciatica and preliminarily explore the mechanism of its action.MethodOne hundred and twelve sciatica patients were randomly allocated to heat-sensitive point Yaoyangguan moxibustion (observation), non-heat-sensitive point Yaoyangguan suspended moxibustion (control) and medication groups. The observation group received heat-sensitive point Yaoyangguan moxibustion; the control group, non-heat-sensitive point Yaoyangguan suspended moxibustion; the medication group, oral administration of Yaobitong capsules. The clinical symptoms were scored, the Pain Rating Index (PRI), the Visual Analogue Scale (VAS) and the Present Pain Intensity (PPI) scores were counted and serum IL-8 content was measured in the three groups beforeand after 15 days of treatment.ResultAfter 15 days of treatment, all the three treatments had a certain therapeutic effect on sciatica. The therapeutic effect was bestin the observation group (P<0.05). The PRI, VAS and PPI scores and serum IL-8 contentdecreased in all the three groups compared with before treatment (P<0.05) and were lower in the observation group than in the control and medication groups (P<0.05). There was no statistically significant difference between the control and medication groups (P>0.05).ConclusionHeat-sensitive point Yaoyangguan moxibustion has a better therapeutic effect than non-heat-sensitive point Yaoyangguan suspended moxibustion and oral administration of Yaobitong capsules. This method is an ideal treatment for sciatica. The mechanism may be related to a posttreatment decrease in inflammatory factors in the diseased region.
7.A Retrospective Analysis of Influenza A(H1N1)in Children of Tianjin
Zaoxian MEI ; Qi WU ; Zhongzhen DU ; Guowei LIU ; Jing YE
Tianjin Medical Journal 2010;38(2):115-117
Objective:To summarize the clinical characteristics of 7 sporadic cases in children of influenza A (H1N1)broken out in Tianjin,and to provide clinical experience and prognosis factors for the diagnosis and prevention of this disease.Methods:The clinical data and protective measures were retrospectively analyzed in 7 children with H1N1 confirmed by Tianjin Haihe Hospital from June 9,2009 to September 21,2009,and the related literatures were reviewed.Results:There were six imported cases of H1N1 and one second-generation case.Fever and cough were the main symptom in children.There were 5 children with hyperpyrexia.Three were with expectoration.Other symptoms included anergy,dizziness,headache,nasal discharge,sneeze and pharyngalgia.Two of these children were with leukocytosis decrease,two were with infection of leukocytosis,and others were normal.Five of these children were with normal chest X-ray,one with texture disorder, and one with bilateral multiple infiltrating image.The positive influenza A virus result was detected by real-time PCR and sequencing comparison in the throat swab samples taken within 2 days from the seven children,and became negative after 5-9 days in the course of this disease.Six patients were accepted osehamivir treatment for 5 days.One was treated only with anti-virus mixture.The average length of hospital stay was 7.9 days.When the children were discharged from hospital,all the symptoms were disappeared.All the patients'families,doctors and nurses were not infected in non-directly exposed to the disease.Conclusion:The seven child patients showed mainly symptoms of fever and cough.The average course was 9.1 days,and they were all cured.Therefore,influenza A(H1N1)is preventable and curable.
8.EFFECTS OF HIGH FAT DIET ON GENE EXPRESSION PROFILE OF INTESTINE IN C57BL/6 MICE
Ruili YANG ; Yonghui SHI ; Wu LI ; Peng YUE ; Guowei LE
Acta Nutrimenta Sinica 2004;0(05):-
Objective To explore the influence of high fat diet on the intestinal gene expression profile in C57BL/6 mice. Method C57BL/6 mice were randomly assigned to two groups (n=8). The control group consumed an ordinary diet. The experimental group was fed with a high fat diet. All mice were sacrificed at the end of 6 w and the intestinal gene expressions were detected by oligonucleotide microarray analysis with Affymetrix GeneChip Mouse U430A consisting of 13 097 genes. Results Among the 13 097 genes obtained from gene expression profile analysis, there were 88 and 179 genes up -and down-regulated respectively, in mice fed with high fat diet compared with the control. The differentially expressed genes were mainly related to free radical oxidative stress, DNA repair, induction of apoptosis, transport, signal transduction and inflammation immune response. Conclusion High fat diet may widely modulate the expression of many genes in the intestine in mice.
9.Studies on chemical constituents in root of Isatis indigotica Ⅲ
Yunhai LIU ; Guowei QIN ; Shuiping DING ; Xiaoyun WU ;
Chinese Traditional and Herbal Drugs 1994;0(02):-
Object To study chemical constituents of the root of Isatis indigotica Fort Methods The powdered plant material was percolated with 95% ethanol, the percolate was extracted with different solvents, the extract was subject to chromatography on silica gel column and macroporous resin column. The compounds were identitfied by their physicochemical properties and spectral data (MS, 1HNMR, 13 CNMR, UV and IR) Results Two compounds were obtained from the ethanol extracts of the plant root They are 3 (2′ hydroxyphenyl) 4(3H) quinazolinone and isaindigodione respectively Conclusion The two compounds were obtained from I. indigotica for the first time
10.Distal tibiofibular synostosis after ankle fracture
Ting LI ; Manyi WANG ; Xieyuan JIANG ; Xinbao WU ; Guowei RONG
Chinese Journal of Orthopaedic Trauma 2002;0(01):-
Objective To report a group of uncommon cases, and discuss t he impact of distal tibiofibular synostosis on the patients and its clinical sig nificance. Methods At a mean follow-up of 22.8 months(11 to 54 months), 14 pati ents with distal tibiofibular synostosis after ankle fracture were evaluated wit h Philips and Schwartz clinical scoring system of ankle. Results 3 patients comp lained of transient pain after strenuous activities. The others complained of no discomfort. All of them had no trouble in normal working and daily activities. The mean degree of plantar flexion was 47.9?, with 3.5?(0 to 10?)less than the normal side. The mean degree of dorsiflexion was 20?, with 8.6?(0 to 20? )less than the normal side. There were no degenerative changes in all ankles. T he mean Philips and Schwartz score was 90.8(82 to 98). The excellent rate was 71 .4%, and the excellent and good rates were 100%. Conclusion Distal tibiofibul ar synostosis after an ankle fracture usually gives rise to few symptoms and nee ds no specific treatment.