Angiogenesis is the process of capillary formation from the existing blood vessels, which is regulated by many cytokines.Balance of these cytokines plays an important role in angiogenesis.Unbalance of these cytokines, lead-ing to excessive or insufficient blood vessel, relates to a variety of diseases, such as tumor, ophthalmic diseases and wound healing.Recently, it has been observed that angiogenesis is also involved in Parkinson’s disease and Alzheimer’s disease. This review mainly discusses the molecular mechanism of angiogenesis and related diseases, and emphasizes the value of targeting angiogenesis as a strategy to develop drugs for those diseases.

AIM: To optimize the milling and extraction rate for Naolisu Granules (Rhizoma Acori Tatarinowii, Radix Notoginseny, Pheretima Radix et Rhizoma Rhei, Radix Curcuma, Radix Ginserg Rubra, etc.). METHODS: The granularity of powder was determined by different micropowder processes. The process was studied by orthogonal design with the content of volatile oil, the yield of extract and the content of emodin. RESULTS: The optimum process of milling was that coarse powder was micropulverized for 20 min at -8℃ ～ -12℃ . The optimum extraction of volatile oil was distillated for 4 hs after volatile medical materials were macerated for 1h with 7 times amount of water. The optimum process of percolation was percolated by 300mL of 75% alcohol with 3.0mL?kg -1 ?min -1 after the medical material powders were macerated for 6 hs. CONCLUSION: These optimum processes are suitable for preparation of Naolisu Granules.

A cDNA encoding novel type III polyketide synthase (PKS) was cloned and sequenced from young leaves of Chinese club moss Huperzia serrata (Thunb.) Trev. by RT-PCR using degenerated primers based on the conserved sequences of known CHSs, and named as H. serrata PKS2. The terminal sequences of cDNA were obtained by the 3'- and 5'-RACE method. The full-length cDNA of H. serrata PKS2 contained a 1212 bp open reading frame encoding a 46.4 kDa protein with 404 amino acids. The deduced amino acid sequence of H. serrata PKS2 showed 50%-66% identities to those of other chalcone synthase super family enzymes of plant origin. The recombinant H. serrata PKS2 was functionally expressed in Escherichia coli with an additional hexahistidine tag at the N-terminus and showed unusually versatile catalytic potency to produce various aromatic tetraketides, including chalcones, benzophenones, phloroglucinols, and acridones. In particular, the enzyme accepted bulky starter substrates N-methylanthraniloyl-CoA, and carried out three condensations with malonyl-CoA to produce 1, 3-dihydroxy-N-methylacridone. Interestingly, H. serrata PKS2 lacks most of the consensus active site sequences with acridone synthase from Ruta graveolens (Rutaceae).

Objective To compare the effect of coronoid fixation combined with lateral collateral ligament repair versus hinged external fixator in treatment of elbow varus posteromedial rotational instability.Methods This retrospective cohort research included 34 patients with elbow varus posteromedial rotational instability operated between January 2011 and June 2015.All patients had coronoid process fractures of O'Driscoll type Ⅱ[(six with subtype 1,24 with subtype 2 and four with subtype 3).Fifteen of the 34 patients were operated by coronoid fixation combined with lateral collateral ligament repair (Group A) and other 19 patients were operated by coronoid fixation combined with placing hinged external fixator (Group B).Interval between injury and operation,operation time and blood loss were recorded.At final follow-up,elbow range of motion,Mayo elbow performance score (MEPS) and Hastings and Graham heterotopic ossification classification were measured.Results There were no significant differences in the interval between injury and operation,operation time and blood loss between the two groups (P ＞0.05).Median period of follow-up was 30 months in Group A and 40 months in Group B.Last follow-up showed flexion of the affected elbow in Group B [145° (135°-150°)] was better than that in Group A [140° (130°-145°)] (P ＜ 0.05),while between-group differences were insignificant in elbow extension,elbow extension-flexion,forearm pronation-supination and heterotopic ossification classification (P ＞ 0.05).MEPS in Group A scored 100 in 10 patients,90 in three,85 in one and 70 in one;MEPS in Group B scored 100 in 13 patients,90 in one,85 in four and 80 in one.There was no significant difference in MEPS between the two groups (P ＞ 0.05).Conclusion For treating elbow varus posteromedial rotational instability,either lateral collateral ligament repair or hinged external fixation after anatomic reduction and coronoid fixation can achieve good results.