Radiotherapy has been an effective non surgical treatment for brain metastases.While the benefit of survival was achieved,the potential cognitive impairment caused by radiotherapy gradually arouse people's attention.To improve patient's prognosis,ensuring tumor control and caring patients' quality of life and neurocognitive functions should be weighed equally.This article reviews the related research on the neurocognitive protection of brain metastasis radiotherapy.

Objective To explore the risk factors,composition of pathogen and drug sensitivity of pulmonary infection in neurosurgical intensive care unit(NICU) and provide basis forclinical effective prevention and appropriate treatment.Methods Retrospective analysis the detected pathogenic strains,theirdrug resistance and predisposing factors of the cases of pulmonary infection in NICU from January 2010 to September 2011.Results Among the isolated 602 bacteriastrains,363 strains (60.2％ ) were G-bacteria,71 strains ( 11.8％ ) were G + bacteria,168 strains (28 ％ )were molds.In G-bacteria,the main strains are acinetobacter and pseudomonas aeruginosa,and in G+ bacteria,staphylococcus epidermidis based most.Conclusion Acinetobacter has become the dominant flora of pulmonary infection in NICU and it has multidrug resistance to antibiotics in clinical.The Specific pathogen should be indentified through bacteriological examination of sputum as soon as possible,and the antibiotics should be used alternatively based on their drug sensitivity to control lung infection in critically ill patients.

Objective To explore the relationship between hepatitis B virus (HBV) S gene variation,genetype and immunoprophylaxis failure to intrauterine infection of HBV.Methods The serum HBV DNA levels of 35 pairs of mother-infants were amplified and quantified by real-time fluorescent quantitative polymerase chain reaction (PCR).Thereafter,the sequences of HBV S gene were determined by sequencing and compared with Genbank standard sequence using DNASTAR software.Results HBV DNA levels of the 35 pairs of mother-infants were all above 1×106 copy/mL.Nucleotide diversity rates were 11.4% in the children and 17.1% in their mothers.The sequence homology between paired mother and infant was beyond 99.3%.The HBV genotype and serotype in 23 pairs of mother-infants was C and adr respectively,while that was B and adw in the other 12 pairs.The HBV genotype and serotype were identical between paired mothers and infants.Conclusions HBV S gene variation may not be a crucial factor for immune failure to HBV intrauterine infection in women with high level viremia.Genotyping could not predict and evaluate the risk of immunoprophylaxis failure to HBV intrauterine infection in neonates.

Objective To explore core competency of nursing students during internship in western China,and to provide reference for the nursing education.Methods 380 nursing students during internship were investigated with Competency Inventory for Registered Nurse (CIRN).Results The total score of core competency of nursing students was (136.40±31.99) points,weighted mean scores of eight dimensions was (2.36±0.51)points;the highest mean score was the dimension of legal and ethical practice (2.88±0.57)points,and the lowest mean score was the dimension of critical thinking (1.91±0.52)points.There was significant difference of core competency in students with different education background and with varied firstchoice profession.Conclusions The core competence of nursing students during internship remains at lower middle level.The nursing educators should shift their concept of nursing education to the new mode of professional competence orientation.

Objective To evaluate the clinical effectiveness of interventional treatment of atrial septal defect (ASD). Methods Transcatheter closure of ASD was performed in 480 cases (198 males and 282 females), age ranged from 1.2 to 72 years (mean 24?8 years). All patients finished clinical examination, chest X-ray, electrocardiography (ECG) and trans-thoracic echocardiography (TTE) for the diagnosis of secundum ASD. The stretch diameter of ASD varied from 6-34 mm, mean 16?4 mm. Four hundred and seventy cases were simple opening secundum ASD. Results The devices were discharged successfully in all patients. The diameter of the occluders selected ranged from 10-40 mm, mean 20?6 mm. Among the patients who had multiple-openings, six cases were closed completely with one occluder, and the other four cases were closed with two occluders. Conclusion Interventional treatment is an effective, safe and optimal choice for the treatment of ASD.

Objective To study the effect of exogenous 3-deoxyglucosone (3-DG) on blood glucose of normal mice. Methods Kunming male mice were divided into two groups: 3-DG (5mg/kg) treatment and control group.At day 1,week 1 and week 2 after treatment, we compared fasting blood glucose,and did the glucose tolerance test in 3-DG group after 2 weeks' 3-DG administration. Results Blood glucose was increased obviously 2h after administration with 3-DG once versus pre-administration and control (7.78±0.694 vs 6.41±0.408,and 6.43±0.43,all higher than control,but no statistical significance (P＞0.05). There were no differences in 6 hour fasting blood glucose after once administration with 3-DG and in 12 hours fasting blood glucose two weeks after administration with 3-DG between 3-DG and Con groups. After 3-DG administration for two weeks,as compared with Con, the 30 minute blood glucose of 3-DG administration mice during OGTT was obviously increased (19.47±1.541 vs 17.22±1.911,P＜0.05), but glucose levels at 60 minute and 120 minute had no changes (P＞0.05). Conclusions Exogenous 3-DG increases the normal mice's blood glucose immediately after once administration with 3-DG and results in up-moving of glucose physiological curve and leads to a light impairment of glucose tolerance when mice were continuously fed with 3-DG for 2 weeks.

Ischemic stroke is closely associated with carotid atherosclerosis.Detecting carotid plaque components (including fibrous Cap,lipid core,hemorrhage,and calcification) using high-resolution 3.0T magnetic resonance imaging contributes to evaluate the risk of ischemic stroke and guide treatment.This article reviews the research status of magnetic resonance imaging of carotid atherosclerotic plaque components.

Stroke-associated pneumonia (SAP) refers to the stroke patients suffering from infectious lung parenchyma (including the alveolar wall,i.e.the general pulmonary interstitial) inflammation without previous pulmonary infection.It is one of the most common major complications resulting in death and affecting functional recovery in patients with stroke.Dysphagia resulting in aspiration is the main reason of SAP.Therefore,avoiding aspiration and preventing SAP have important significance for patients with stroke through early correct tube feeding.This article reviews the tube feeding methods of preventing SAP.

Transient ischemic attack (TIA) traditionally refers to temporary brain dysfunction lasting no longer than 24 hours due to a shortage of blood and oxygen, without any residual neurological deficit. In recent years, the development of imaging technology enables us to have a new awareness about TIA. This article reviews the effects of MRI, especially diffusion-weighted imaging, in the diagnosis and prognostic prediction of TIA.

Objective To investigate the beneficial effect on enterocyte-like cells HT-29 through adhesion of Lactobacillus. Methods Using viable bacteria counting method to evaluate inhibition of Escherichia coli adhesion and invasion to enterocyte-like cells by incubation with Lactobacillus together. Cellular membrane permeability and cell viability were examined in vitro by LDH level and MTT method respectively. Results Adhesion and invasion of enteropathogenic Escherichia coli to enterocyte-like cells was inhibited by high adhesiveness strain Lactobacillus reuteri JCM1081. In vitro, no significant changes of the morphology, structure and function of the HT-29 cells after being treated with Lactobacillus alone for 3 hours, especially cellular LDH level, viability and membrane permeability. Lactobacillus reuteri JCM1081 did not alter cell integrity and prevented the increase in permeability induced by enteropathogenic Escherichia coli infection. Conclusion Lactobacillus reuteri JCM1081 interact with intestinal epithelial cell receptor to competitively inhibit the adhesion and invasion of enteropathogenic Escherichia coli to HT-29 cells by incubation and enhance the integrity of the cells.