1.Effect of pre-operation intestinal preparation by orally taking magnesium sulfate of two different concentration
Chinese Journal of Practical Nursing 2010;26(20):30-33
Objective To explore the effect of pre-operation intestinal preparation by orally taking magnesium sulfate of different concentrations. Methods 50 patients were divided into the high-concentration group and the low - concentration group, according to the concentration of magnesium sulfate orally taken for pre-operation intestinal preparation. Some factors were compared between the two groups including: the acting amount of water, starting and lasting timing of magnesium sulfate, times of stool, the efficiency of the intestinal preparation, the tolerance, uncomfortable reaction and changes of serum electrolyte of the patients. Results There were no difference between the two groups on the acting amount of water, starting and lasting timing of magnesium sulfate, times of stool, the efficiency of the intestinal preparation, the tolerance of the patients. The rate of the stool without solid substance was higher in the high-concentration group than the low-concentration group(40% vs 8%). The uncomfortable reaction rate was also higher in the high-concentration group, the concentration of serum electrolyte decreased in both groups, among which serum Ca2+ decreased significantly in the low-concentration group. Conclusions Orally taking magnesium sulfate of different concentrations both has effect on bowel preparation, influence on patients' serum electrolyte is remarkable.
2.Quality standard of Haimo Blood-Stanching Tablets
Chinese Traditional Patent Medicine 1992;0(06):-
AIM: To establish the quality standard for Haimo Blood-Stanching Tablets(cuttle fish sepia) by means of quantitative and qualitative experiments. METHODS: Melanin was identified by physics and chemistry reaction.Gly,Asp,Glu and Ala in Haimo Blood-Stanching Tablets were determined by HPLC-AccQ-Tag method and iron was determined by atomic absorption spectrophotometer. RESULTS: Melanin in sepia could be dissolved in 2 mol/L NaOH and there was something black separated out when in 2 mol/L HCl.The recoveries of four kinds of(amino) acids and iron were all in the range of 95%-105%.Each RSD was below 5%(n=6). CONCLUSION: The quantitative and qualitative analysis methods are accurate and reproducible.This study provides a method for the quality control of Haimo Blood-Stanching Tablets.
3.Pharmacokinetics of isosorbide-5-mononitrate sustained-release capsules in healthy volunteers
Guorong FAN ; Jinhong HU ; Mei LIN ;
Academic Journal of Second Military Medical University 1985;0(05):-
Objective:To study the pharmacokinetics and relative bioavailability of isosorbide 5 mononitrate(5 ISMN)sustained release capsules in 12 male healthy volunteers. Methods: A capillary gas chromatography method with electron captured detector was established for the determination of 5 ISMN in plasma, after administrating a daily dose of 40 mg 5 ISMN domestic and imported sustained release capsules in a randomized crossover design.The fluctuation of 5 ISMN valley concentrations observed and the steady state pharmacokinetics was characterized in a multiple dose schedule of 40 mg 5 ISMN sustained release capsules.Results:The steady state of 5 ISMN plasma concentrations reached at the fourth oral administration.The steady state pharmacokinetic parameters after multiple oral dose of 40 mg 5 ISMN domestic and imported sustained release capsules were as follows: t max were (4.8?0.6) and (4.8?0.9) h, c max were (802.46?94.45) and (807.09?109.16) ?g/L, c min were (127.83?23.76) and (121.85?22.00) ?g/L, AUC ss were (8 114.7?1 393.4) and (8 174.8?1 219.7) ?g?h/L, c av were (338.12?58.06) and (340.62?50.82) ?g/L,the degree of fluctuation (DF) were (2.04?0.39) and (2.03?0.33), respectively. Conclusion:The relative bioavailability of multiple oral dose of 40 mg domestic 5 ISMN sustained release capsules is (99.07?5.45)%, there are no significant differences in the main steady state pharmacokinetic parameters between domestic and imported sustained release capsules, and so the 2 capsules are bioequivalent.
4.In situ rat intestinal absorption of hawthorn leaves flavonoids
Yingai XU ; Guorong FAN ; Shen GAO ; Zhanying HONG
Chinese Traditional Patent Medicine 1992;0(12):-
0.05).But the P_ eff values were significantly increased in the presence of P-glycoportein(P-gp)inhibitor,verapamil or digoxin.CONCLUSION:Rhamnosylvitexin can be classified into high penetrating drug.Passive diffusion dominates the absorptive transport behavior of rhamnosylvitexin in HLF.There is not a preferential absorption zone in the intestine for rhamnosylvitexin.The absorption and secretion of rhamnosylvitexin in HLF are mediated by the efflux transport system,P-gp.
6.Determination of osthol in LOTIO FRUCTUS CNIDII COMPOSITA by RP-HPLC
Xinyu LIU ; Fang WU ; Guorong FAN ; Jinhong HU ;
Chinese Traditional and Herbal Drugs 1994;0(09):-
Object To estabish a method for the quantitative analysis of osthol in LOTIO FRUCTUS CNIDII COMPOSITA by RP HPLC Methods Megestrol acetate was used as the internal standard Results Chromatographic separation of osthol and megestrol acetate was accomplished within 12 min on Hypersil ODS 2(200 mm? 4 0 mm, 5 ?m) column and acetonitrile 0 01 mol/L sodium dihydrogen phosphate (48∶52) as mobile phase The flow rate was 1 2 mL/min and the detection wavelength was 320 nm A good linearity was obtained in the range of 0 123 8~2 970 ?g/mL (r=0 999 6) for osthol and the average method recovery was (98 4?0 90) % with RSD=0 91% Conclusion The method was simple, rapid, accurate, reliable, and suitable for the quality control of LOTIO FRUCTUS CNIDII COMPOSITA
7.Rapid and undamage determination of fruit of Eucalyptus globules labill by near infrared spectroscopy and clustering analysis
Yuming LIU ; Yifeng CHAI ; Yunpeng QI ; Guorong FAN ; Yutian WU ;
Chinese Traditional Patent Medicine 1992;0(12):-
AIM: To provide a rapid method for identifying fruit of Eucalyptus globule labill. METHODS: A new method, using NIRS for rapid and nondestructive classfication of the fruit of Eucalyptus globules labill and its counterfeit drug, the fruit of Eucalyptus robusta Smith, was proposed and then cluster analysis was adopted. RESULTS: The result showed that two kinds of fruits had their own characteristic NIRS spectra. CONCLUSION: The method is quick, simple and reliable, and in combination of the fingerprint NIRS spectrum and the clustering analysis is easy to identify the adulteration.
8.Simultaneous Determination of Four Lignans in Magnoliae Flos Extract by High Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry
Xin ZHAO ; Guang YANG ; Guoshuai ZHENG ; Taijun HANG ; Guorong FAN
Chinese Journal of Analytical Chemistry 2014;(12):1804-1810
A high performance liquid chromatography-electrospray ionization mass spectrometry- charged aerosol detection ( HPLC-MS-CAD) method was established for the simultaneous quantitative analysis of four Lignans in Magnoliae Flos extract. The components were separated on a YMC-Pack ODS-A column (250 mm× 4. 6 mm, 5 μm) by gradient elution with methanol and water as the mobile phase at aflow rate of 1. 0 mL/min. Then the elution solution was routed into MS equipment at a flow rate of 0. 3 mL/min and CAD detector at a flow rate of 0. 7 mL/min by a split ratio of 3:7 for the further detection. The column temperature was 25 ℃ and the detection wavelength was 278 nm. A method was developed for the quantitative analysis of muti-components by single maker ( QAMS) to determine pinoresinol dimethylether, magnoli, 1irioresinol B dimethylethe and epi-magnoli A . Magnoli was selected as internal standard and the relative correction factors ( RCF) of the four Lignans were calculated. The contents of the four Lignans in Magnoliae Flos extract were determined by both external standard method and QAMS. The QAMS method was evaluated by comparison of its assay result and that of external standard method. Under the selected chromatographic condition, the limits of detection of pinoresinol dimethylether, magnoli, lirioresinol B dimethylethe and epi-magnoli A were 0. 34, 0. 55, 0. 50 and 0. 58 mg/L, respectively, while the linear range were within 6. 8-270 mg/L, 11-546 mg/L, 2. 0-101 mg/L and 2. 3-116 mg/L. The recoveries ( n=9 ) were 98. 2%-99. 5%, and the correlation coefficient were 0 . 9995-0 . 9998 . No significant differences were found between the quantitative results of external standard method and QAMS method. The developed method is accurate, feasible, and can be used for quality evaluation of Magnoliae Flos .
9.Absorption kinetics of breviscapine in intestine of rats in situ
Yingai XU ; Guorong FAN ; Shen GAO ; Zhanying HONG
Chinese Traditional and Herbal Drugs 1994;0(07):-
Objective To investigate the intestinal absorption kinetics of breviscapine in rats.MethodsThe intestinal absorption in small intestine and colon of rats in situ was investigated using circular perfusion and HPLC methods.The in situ absorption of scutellarin in the small intestine was studied and the effects on the intestinal absorption were observed at the various concentrations and different pH values using the same methods.Results The results showed that there were significant differences in both absorptive percentage and absorption rate constant(ka)in the small intestine between the experimental groups of rats with and without bile duct being ligated.The absorption rate constants in small intestine and colon were(0.107 1?0.013 0)and(0.070 7?0.008 9)h-1,respectively.No saturation phenomenon occurred and ka was almost kept unchanged at different concentrations of breviscapine.The absorption of breviscapine was not influenced by pH values ranging from 6.0 to 7.4.Conclusion The results indicates that breviscapine is absorbed more in small intestine than in colon.The absorption of breviscapine complied with the passive diffusion mechanism and first order kinetics.In conclusion,these results suggest that breviscapine could be prepared in oral sustained-release dosage form.
10.HPLC-DAD/ESI-MS in analyzing chemical constituents of Rhizoma belamcanda
Chao FENG ; Tingting ZHOU ; Guorong FAN ; Hua WEI
Academic Journal of Second Military Medical University 2000;0(07):-
Objective:To analyze the chemical constituents of Rhizoma belamcandae by using high-performance liquid chromatography-diode array detector/electrospray ionization-mass spectrometry (HPLC-DAD/ESI-MS).Methods: Dried Belamcanda chinensis powder was extracted with 70% ethanol by sonication.The chromatographic separation was performed on a YMC ODS-C18 column (250 mm?4.6 mm I.D.,5 ?m) with a mobile phase composed of 20%CH3OH(A)-70%ACN(B) (0→30→45→65 min,20→30→90→100 B),eluted at a flow rate of 0.45 ml/min,and the UV detection wavelength was set at 265 nm.Positive ionization mode with a needle voltage of 5 000 V,a capillary voltage of 20 V,a gas(N2)press of 20 psi and a temperature of the drying gas of 300℃ was selected.Relative molecular mass data acquisition was performed from m/z 250 to 550 in full MS scan mode.Results: Nine major isoflavones were identified from Rhizoma belamcandae based on their retention behavior obtained on-line by their UV spectra and the HPLC-DAD/ESI-MS.Conclusion: A rapid and efficient HPLC-DAD/ESI-MS method for identifying the chemical constituents of Belamcandae chinensis has been established,which provides more scientific information for quality control of Rhizoma belamcandae.