Objective To evaluate the effect of cervical conization on the outcome of subsequent fertility and adverse pregnancy.Methods 87 cases of cervical intraepithelial neoplasia (CIN)patients cured by cervical coniza-tion were choosed as observation group.And randomly 90 cases women from pregnancy clinic were choosed as control group.the pregnancy outcomes were compared beween the two groups.Results The observation group:pregnancy rate was 88.5%(77 cases).The control group:pregnancy rate was 91.1%(82 cases)(P >0.05).The difference was not statistically significant.The observation group:premature rate was 14.9%(13 cases)and PROM rate was 17.2%(15 cases),which were higher than 6.7% and 8.9% in the control group.The differences were statistically significant (χ2 =3.45,3.04,all P <0.05).There was no significant difference in the abortion rate and cesarean section rate. (χ2 =0.02,0.15,all P <0.05)Conclusion Cervical conization has no obvious effect on the ability of pregnancy, abortion rate and cesarean section rate,but the premature rate and rate of premature rupture of membranes increase.

Objective To establish the prevalence of renal involvement in a large group of patients with type 2 diabetes and evaluate several risk factors of early stages and advanced stages of albuminuria. Methods Five hundred and sixty-six patients(265 males, mean age:57.8?13.3 years, diabetes duration 8.35?6.4 years;301 females, mean age:60.0?12.4 years, diabetes duration 9.9?7.3 years) from the endocrinology unit of Beijing Tongren hospital were included in this study. Urinary albumin excretion rate(UAER),age ,diabetes duration, arterial blood pressure, body mass index(BMI),waist to hip ratio(WHR),fasting blood glucose(FBG),HbA1c,lipid profile were evaluated. Results The prevalence of microalbuminuria, macroalbuminuria was 24.6% and 16.8% respectively. On logistic regression analysis, using the presence or absence of microalbuminuria as dependent variable, arterial blood pressure and, fasting blood glucose were included in the model in both males and females. When analyzing patients with microalbuminuria vs those with macroalbuminuria, in females, arterial blood pressure and triglycerides were included in the model. In males, only blood pressure was included in the mole. Conclusions High fasting blood glucose and arterial blood pressure were independent risk factors of microalbuminuria in type 2 diabetic patients .High arterial blood pressure and disorder in lipid profile were independently associated with macroalbuminuria.

Objective To explore the inhibitory effect of anti-miRNA-214 oligonucleotide on leukemia U937 cells.Methods U937 cells were transfected with anit-miRNA-214 oligonucleotide,cell viability was analyzed by MTT assay.Apoptosis was detected by flow cytometry.The expression of miRNA214 in the U937 cells were measured by real-time PCR.Results The MTT result showed that the growth of U937 cells treated with AMO-miRNA-214 in 24 h,48 h,72 h was obviously inhibited (0.812±0.001,0.770±0.002,0.541±0.001),compared with those in control groups (randomized control 1.011±0.002,1.112±0.003,1.111±0.003,blank control 1.112±0.001,1.023±0.001,1.101±0.001),the differences had statistical significance (F =2.782,3.659,2.735,P =0.021,0.018,0.036).The flow cytometry results showed that the apoptosis detected in AMO-miRNA-214 group at 48 h,72 h (15.12±0.02,19.14±0.01) had significant differences compared with those in control groups (randomized control 2.04±0.02,2.45±0.03,blank control 1.19±0.02,2.02±0.01) (F =3.683,3.762,P =0.013,0.015).The expression of miRNA-214 was downregulated significantly in U937 cells after treated with oligonucleotide (31.1±0.2) compared with those in control groups (randomized control 25.8±0.1,blank control 25.6±0.2) (P ＜ 0.05).Conclusion Targeted inhibition of miRNA-214 with oligonucleotide can suppress U937 cells growth and induce apoptosis.

BACKGROUND: Decoy receptor 1 (DcR1), which acts as decoy receptors, is resistant to apoptosis induction by related apoptosis inducing ligand (TRAIL). OBJECTIVE: To investigate the expression of the DcR1 (TRAIL-R3) protein in human herniated and normal lumbar intervertebral discs (IVD). METHODS: The expression and distribution of DcR1 proteins were assessed by using immunohistochemistry in 20 herniated lumbar IVD and 8 normal lumbar IVD tissues samples following discoidectomy from January to September 2010. RESULTS AND CONCLUSION: Percentage of nucleus pulposus and anulus fibrous cells with DCR1 expression was obviously more in the herniated group than in normal IVD group. The current results indicate that the expression of DCR1 rises after herniation.

Objective To explore the effects of embolizing intracranial ruptured aneurysm in early stage using Guglielmi Detachable Coil (GDC). Methods 10 intracranial ruptured aneurysms including 8 cases of the first ruptured and 2 cases of the second ruptured were studied. The locations of aneurysm were as following: 1 case in anterior cerebral artery, 1 case in middle cerebral artery, 5 cases in anterior communicating artery, 2 cases in posterior communicating artery, and 1 case of multianeurysm in anterior and posterior communicating artery. Hunt-Hess grade was as following: 2 cases of gradeⅠ,4 cases of gradeⅡ, 2 cases of gradeⅢ, 1 case of grade Ⅳ, and 1 case of grade Ⅴ. Fisher CT scale was as following: 2 cases of gradeⅠ, 6 cases of gradeⅡ,and 2 cases of grade Ⅲ. Embolization of intracranial ruptured aneurysm was performed in early stage using GDC, 7 cases in 3 days after ruptured, and 3 cases in 6 days after ruptured. Results Among 11 aneurysms in 10 cases, 8 cases were 100% embolization, 2 cases≥95%, and 1 case≥90%. Followed-up from 1 month to 2 years after operation 1 case was dead, 7 cases recovered well, 1 case was in vegetable state, and 1 case occurred subarachnoid hemorrhage again. Conclusions GDC early embolization is preferable technique for treating intracranial ruptured aneurysm.

Objective To study the relationship between the polymorphism of N 5,10 methy lenetetrahydrofolate reductase (MTHFR) gene and the susceptibility to diabetic microangiopathy (DMAP) in type 2 diabetes mellitus (DM) of Han nationality in north China. Methods By PCR RFLP, MTHFR C677T mutation was detected in 229 type 2 diabetic patients, including 102 non complication diabetics (NCD group), 67 diabetic nephropathy (DN group) and 60 diabetic retinopathy (DR group), and 62 healthy persons as control (CON group). Results The frequency of homozygous BB genotype and allele B in DN and DR were significantly higher than that in NCD and CON, but NCD and COD showed similar frequency of BB genotype and allele B. Conclusion In type 2 DM of Han nationality in north China, there is a strong correlation between the polymorphism of MTHFR gene and DMAP, and the allele B may be a susceptibile gene of DMAP.

From 1965 to 1986, 36 patients with Wilms' tumor were terated by nephrectomy,postoperative irradiation and chemotherapy. The radiation doses of 1540～3920cGy were given according to an age-medulated dose scheme. All except 4 Stage I received chemotherapy. The most commonly used chemotherapeutic agent was Act-D, VCR. The overall 5-year survival rate was 61.1%(22/36) and 10-year survival rate was 56%(14/25). The 5- and 10-year survival rates were 87.5% and 80% for Stage I, 63.2% and 61.5% for stage II,33.3% and 28.6% for stage III patents. The favorable factors which led to better results were: early stages(stages I,II) and aged under 5 years . The 5-year survival rates of Stages I,II and Stage III were 70.4%(19/27) and 33.3%(3/9), respectively(P

Objective To observe clinical effects of fleabane injection treating patients with unstable angina pectoris (UAP). Methods One hundred and sixty cases with UAP were randomly recruited into two groups: a control group (including 80 patients) received Asprin and Nitrate medication, and a treatment group(including 80 patients) received fleabane injection (20 mg/d, ivdrip) on the basis of the control group. Both groups were treated for two weeks. Results Compared with the control group, the treatment group showed significantly better clinical effects and electrocardiograph. Conclusion Fleabane injection has obvious clinical effects for treating patients with UAP.

Objective To discuss the changes of plasma TXA2 and PGI2 levels in the rabbit model of pulmonary hypertension (PHA)induced by monocrotaline (MCT)when transplanted with endothelial progenitor cells (EPCs). Methods The rabbit pulmonary hypertension model was constructed by MCT induction. Rabbit endothelial progenitor cells were induced,separated and identified in vitro.50 New Zealand white rabbits were randomly divided into 3 groups,normal control group and model group were given M199 culture medium by tail vein injection,EPCs treatment group were given EPCs marked with fluorescent though tail vein injection. The levels of plasma TXB2 and 6-keto-PGF1αwere detected and their ratio were calculated after three weeks. Results Compared with model group,the content of 6-keto-PGFlαin plasma in EPCs group was (130.67 ±17.54)u mol/L,which was increased sinnificantly. The content of TXB2 was (402.89 ±27.98 )u mol/L,which was decreased significantly.The ratio of TXB2 to 6-keto-PGF1 alpha between two groups was statistically significant(P<0.01 ). Conclusion EPCs transplantation can reduce the content of TXA2 and increase the content of PGI2 in the pulmonary hypertension rabbits induced by monocrotaline,then reverse the damage of pulmonary hypertension.