Background and purpose:Backgrounds and Objective: Oxaliplatin is a third-generation platinum compound,there are some distinct differeuces in mechanism between cisplatin and oxaliplatin,and oxaliplatin is less toxic Poorly differentiated cartinoma is the most common histological type,for nasopharyngeal carcinoma,although radiotherapy is the basic therapeutic approach to nasopharyngeal carcinoma,chemotherapy is also important for recurrent and metastatic nasopharyngeal carcinoma,so in this work we analyzed the effects of oxaliplatin on nasopharyngeal carcinoma cell lines in vitro.Methods:CNE2 was incubated with oxaliplatin at various concentrations and times,cell growth inhibition was assessed by MTT-microculture tetrazolium assay,cell-cycle kinetics and apoptosis were analyzed by flow cytometry and microscopy transmission electron.Results:Growth of CNE2 cells was significantly inhibited in a dose-dependent and time-dependment fashion.The inhibition of cell growth inhibition was(95.6?0.7)% after incubation with 100 ug/ml oxaliplatin for 48 hours,the cell was arrested at G_(2)/M and apoptosis was induced.When CNE2 cells were treated with oxaliplatin at the concentration of 0、0.03、4.0、100 ?g/ml,the rates of CNE2 cell apoptosis were(0.19?0.17)% 、(0.37?0.09)%、(5.50?1.08)%、(9.43?0.09)%,respectively.24 hours after 20 ?g/ml the CNE2 cells were characterized by chromatin condensation,chromatin crescent formation,nucleus fragmentation and apoptosis body by transmission electron microscopy.Conclusions:Oxaliplatin suppresses the growth of CNE2 cells in vitro by causing cell-cycle arrest and cell apoptosis.

Objective To explore the expression of FLT3 on the cells from acute myeloblastic leukemia (AML)and its clinical signficance.Methods The expression of FLT3 from 80 AML cases was measured by FISH.All cases were indentified by karyotyping,40 cases were normal karyotye,and the other 40 cases were abnormal.Results The FLT3 expression level was different in different AML cases.The expression rate in normal karyotype cases was 5.0%,but in abnormal cases 30.7%,in refractory relapse cases 43.3%,in continual complete remission cases 4.5%.The patients’DFS (Disease-Free survival)and OS (overall survival)without FLT3 expression were 65% and 86% in 24 months’following investigation,but the patients’ DFS and OS with FLT3 expression were 20% and 40% respectively.The difference was distinct between them(P<0.05). Conclusion FLT3 can be thought as a new marker for worse prognosis of AML patients,and its expression level is associat-ed with maglignacy-grade.

Objective To explore the inhibitory effect of anti-miRNA-214 oligonucleotide on leukemia U937 cells.Methods U937 cells were transfected with anit-miRNA-214 oligonucleotide,cell viability was analyzed by MTT assay.Apoptosis was detected by flow cytometry.The expression of miRNA214 in the U937 cells were measured by real-time PCR.Results The MTT result showed that the growth of U937 cells treated with AMO-miRNA-214 in 24 h,48 h,72 h was obviously inhibited (0.812±0.001,0.770±0.002,0.541±0.001),compared with those in control groups (randomized control 1.011±0.002,1.112±0.003,1.111±0.003,blank control 1.112±0.001,1.023±0.001,1.101±0.001),the differences had statistical significance (F =2.782,3.659,2.735,P =0.021,0.018,0.036).The flow cytometry results showed that the apoptosis detected in AMO-miRNA-214 group at 48 h,72 h (15.12±0.02,19.14±0.01) had significant differences compared with those in control groups (randomized control 2.04±0.02,2.45±0.03,blank control 1.19±0.02,2.02±0.01) (F =3.683,3.762,P =0.013,0.015).The expression of miRNA-214 was downregulated significantly in U937 cells after treated with oligonucleotide (31.1±0.2) compared with those in control groups (randomized control 25.8±0.1,blank control 25.6±0.2) (P ＜ 0.05).Conclusion Targeted inhibition of miRNA-214 with oligonucleotide can suppress U937 cells growth and induce apoptosis.

Objective To study the clinical characteristics of elderly sleep apnea syndrome (ASA). Methods 329 SAS patients diagnosed by 7-hour polysomnography(PSG) were classified into the aged and the middle aged groups. Results Both the body mass index(BMI) and neck circumference were significantly lower in the aged group than those in the middle aged group(P

OBJECTIVETo observe the clinical efficacy on cognitive impairment after traumatic brain injury (TBD treated with acupuncture and cognitive training.

METHODSSixty patients were randomized into an observation group and a control group, 30 cases in each one, and 5 cases of them were dropped out due to the earlier discharge. Finally, there were 28 cases in the observation group and 27 cases in the control group. In the control group, the cognitive training and conventional treatment were applied. In the observation group, on the basis of the treatment as the control group, acupuncture was applied to Baihui (GV 20), Fengchi (GB 20), Geshu (BL 17) and Fenglong (ST 40), once a day, for 4 weeks totally. The mini-mental state examination (MMSE) and Montreal cognitive assessment (MoCA) were adopted to evaluate the cognitive function in the patients of post-TBI cognitive impairment.

RESULTS(1) After treatment, the total score in MMSE and the score of each item were increased significantly as compared with those before treatment in the two groups (all P<0. 05). Except for the score of immediate recall, the score in MMSE and the score of each of the other items were increased significantly in the observation group as compared with those in the control group after treatment (all P<0. 05). (2)After treatment, the total score in MoCA and the score of each item were increased significantly as compared with those before treatment in the two groups (all P<. 05). Except for the score of nomenclature item, the total score in MoCA and the score of each of the other items were increased significantly in the observation group as compared with those in the control group after treatment (all P<0. 05).

CONCLUSIONBoth the simple cognitive training and the combined therapy of acupuncture and cognitive training improve MMSE and MoCA scores and relieve the cognitive impairment induced by TBI. But the combined therapy achieves the much better efficacy.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Brain Injuries ; psychology ; therapy ; Cognition ; Cognition Disorders ; psychology ; therapy ; Cognitive Therapy ; Female ; Humans ; Male ; Middle Aged ; Treatment Outcome

Objective To observe the clinical efficacy of acupuncture and moxibustion combined with cognitive training in treating cognitive impairment after traumatic brain injury (TBI). MethodsSixty patients were recruited into the study and randomly divided into the control group and the treatment group according to the MINIMIZE software. Patients in the control group were treated with cognitive training and regular treatment. Besides the traditional treatment, patients in the treatment group were additionally treated with acupuncture and moxibution. The treatment lasted four weeks. Mini-Mental State Examination (MMSE) and Loewenstein Occupational Therapy Cognitive Assessment (LOTCA) were applied to evaluate the patients’ cognitive function before and after the treatment.Results In the end, there were 27 patients in the control group and 28 patients in the treatment group, because 5 patients withdrew from the study. After treatment, scores of MMSE, LOTCA and their sub-items in the control group and the treatment group increased significantly (P<0.05), and the scores in the treatment group were higher than the control group (P<0.05).Conclusion Combination of acupuncture, moxibution, and cognitive training could help patients after TBI to increase the scores of MMSE and LOTCA, and improve the cognitive impairment caused by TBI. Its therapeutic effect is superior than the pure cognitive training.

Objective To explore the curative effect of ursodeoxycholi acid on retinopathy of prematurity (ROP) caused by high concentration of oxygen in newborn rats. Methods The model of ROP was established. Neonatal rats were divided into normal control group, ROP model group, low dose ursodeoxycholi acid treatment group (10mg/kg) and high dose ursodeoxycholi acid treatment group (40mg/kg). Rats were sacrificed at days 17. The new retinal vessels were observed and counted under lfuorescence microscope. Results The new retinal vessels in ROP rats were hyperplastic, twisted and unevenly distributed. There was signiifcant difference in the number of new retinal vessels among different groups (P=0.000). The number of new retinal vessels of rats in ROP group, low-dose group and high-dose group was signiifcantly more than that in control group (P=0.000). The number of new retinal vessels in low-dose group and high-dose group was significantly less than that in ROP group (P<0.05). The number of new retinal vessels in high-dose group was less than that in low-dose group (P>0.05). Conclusions Ursodeoxycholi acid could inhibit the angiogenesis of retina and could have curative effect on ROP.

Objective To improve recongnition of CT manifestations and value in diagnosing aortic dissection and rupture.Methods The CT plain and enhanced findings in 16 cases with aortic dissection were analyzed retrospectively,these 16 cases were classified in to DeBakey type Ⅰ?Ⅱand Ⅲ in 2,4 and 10,in which 3 cases had subacute and chronic rupture.Results Among these 16 patients with AD,the aortic double lumen and intimal flap,6 cases were showed minor calcifications in the dissection membrane,3 cases were acute rupture or slow leakage complications including periaortic mediastinal,pericardiac pleural or retroperitoneal effusion and retroperitoneal diffuse fibrosis.Conclusion CT is a helpful reliable and rather simple technique for AD lesion and complication.It should be suggested if a diffuse fibrosis occure in retroperitoneal space complicating with chronic permeability of AD.

Bile collected from 28 cases with various hepatic or biliary diseases during operation was assayed for the ?-glucuronidase level. In Group I consisting of 14 patients with primary intrahepatic stone, the ?-glucuronidase level averaged 2250.67 u/dl, while in Group II consisting of 14 patieats with other hepatic diseases, it averaged only 463.99 u/dl(P = 0.01).The patients of both groups showed a declination of the enzyme level gostoperatively, but in Group I, the enzyme level raised with other hepatic diseases, he T-tube. The AMPS and MP contents of the bile .remarkable inhibitory effect on two substances exert their function in positive remarkable inhibitory effect on at pH4,6. It is believed that. ?-glucuronidase, Group 11 consisting the results of thisles in the reformation of biliary stones reformation biliaryWhen ?-glucuronidase level is lower than 200u/dl.Chinese traditional prescriptions with glycynrhizae liquid extract shows a remarkable inhibitory effect on this enzyme. In Chinese traditonal prescriptions, the drugs which can promote circulation and clear deposit are usually added. According to the results of this study, the authors suggest that the therapeutic effect of glycyrrhizae be emphasized.

Objective To design a speech command module to replace manual command during X-ray examination.Methods The module was composed of the parts for speech,pushbutton actuator,speaker,miniature voice box,power source and etc.The electrical conductors were used to connect the above parts,and the speech command was formed by special software.Results The module had the speech command triggered by the pushbutton,and the speech command was pellucid and clear so that the workload could be decreased.Conclusion The module facilitates the speech command during X-ray examination,and thus is worthy promoting practically.