This paper reviews progresses made in China from 2011 in areas of "Synthetic Biology" supported by State Basic Research 973 Program. Till the end of 2014, 9 "synthetic biology" projects have been initiated with emphasis on "microbial manufactures" with the 973 Funding Program. Combined with the very recent launch of one project on "mammalian cell synthetic biology" and another on "plant synthetic biology", Chinese "synthetic biology" research reflects its focus on "manufactures" while not giving up efforts on "synthetic biology" of complex systems.
Animals ; China ; Plants ; Synthetic Biology

This paper reviews the research and commercialization progresses of biobased polymeric materials including polyhydroxyalkanoates (PHA), polylactides (PLA), poly (butylene succinate) (PBS) and its monomer succinate, and CO2 copolymer poly (propylene carbonate), especially these efforts made in China.
Bioengineering ; Butylene Glycols ; China ; Polyesters ; Polyhydroxyalkanoates ; Polymers ; Propane ; analogs & derivatives ; Succinic Acid

Objective To test the hypothesis that if oxidized low density lipoprotein (ox-LDL) were associated with target organ damage(TOD) in patients with essential hypertension.Methods A total of 182 patients with primary hypertension[102 males and 80 females,age (52.8?18.4) years] were enrolled in this study in our department between October 2006 and June 2009.Patients were classified as with TOD(n=85) or without TOD (n=97).Left ventricular mass index (LVMI) and intima-media thickness(IMT) was determined using echocardiography;24 hours urine microalbumin and retinal changes were measured.Cerebral hemorrhage,ischemic infarction,transient ischemic attack (TIA) were evaluated by CT or MRI.Serum ox-LDL was measured by sandwich ELISA.Results Mean ox-LDL level were (114.6?24.3)U/L in without target organ damage patients which was significantly lower than that in target organ damage group[(164.2?27.6)U/L,P

0.05).As for patients' age the old age group had the statistical significance vs the non-old age group(P

Objective To improve the mammographic diagnostic level of breast ductal diseases.Methods X-ray features,operationand pathologic findings in 166 cases of breast disease were retrospectively analyzed.Results The galactography of all 166 cases showed the manifestations including 78 cases of mammoductal ectasis with ductal dilatation,smooth wall;2 cases of ductal ectasis complicated by infection with ductal dilatation,inregular coarsening wall and interstitial extravasation of contrast medium;11 cases of mammoglandular cystic lobular hyperplasia with cyetic dilatation of terminal ducts in different sizes;49 cases of intraductal papilloma with sound as ovoid fillingdefects showing as interrupted smooth bordered,"cupping" in 26 cases of intraductal papillary carcinoma or intraductal carcinoma with post dilatation ductal stenosis and sigid "amputation" absuption together with sound cauliflower or inregular filling defects.In this series of 166 cases,75 cases vice prover to be tumor(45.1%) with malignancy in 26 cases(15.7%).Conclusion Galactography is an important method for diagnosis and differential diagrosis of mammoglandular disease with nipple discharge.

Objective To evaluate the effect of preoperative chemotherapy on bulky cervical cancer by internal iliac arterial infusion. Methods One hundred and eighty-six patients with bulky cervical cancer were randomly divided into two groups: chemotherapy + radiotherapy group (C+R group, n=105) and radiotherapy group (R group, n=81). Patients in C + R group underwent internal iliac arterial infusion chemotherapy by using Seldinger technique internal iliac arterial or epigastric arterial catheterization. Combined regimens were prescribed including cisplatin as the major drug. Meanwhile ~192 Ir high-dose-rate intracavitary radiotherapy was performed,with A point dose at ~12-24 Gy/2-4 times (C + R group). Patients in R group were only given radiotherapy. Both groups of patients received radical hysterectomy two weeks after radiotherapy. Results The tumor regression rate of C+R group was 97.1%, significantly higher than 79.0% in R group(P0.05). Postoperative pathologic examinations showed the percentage of cervical tumor residue, parauterine invasion,pelvic lymph node metastasis in C+R group was lower than those of R group(P

Objective To evaluate the diagnostic approaches and treatment choices for priapism,and to upgrade the diagnostic and therapeutic efficacy. Methods Thirteen patients with priapism were evaluated by physical examinations,cavernous blood gas analysis,color duplex ultrasonography and cavernosography.Among them 9 cases of priapism resulted from intracavernosal injection of vaso-active agents,1 from perineal trauma and 3 from unknown cause.All the 13 patients were treated with intracavernosal injection of aramine and cavernosal decompression. Results Among these cases of priapism,12 were of low-flow and 1 was of high-flow.After the treatment,all the patients with priapism achieved remission.During the follow-up of 3 to 43 months,4 cases experienced decreased erection;9 had no significant change of erection.No cavernous fibrosis of penis occurred in them. Conclusions Cavernous blood gas analysis and color duplex ultrasonography are helpful with the accurate and timely dignosis of priapism.Cavernosal decompression and intracavernosal injection of arimine can be applied to most of the patients,and in the course of the treatment systemic adverse events should be avoided.

Objective:To search for common epitopes of(H5N1) from different areas in the world in order to develope epitope-bacterin by the way of immunoinformatics.Methods:Firstly,homologous degree of the amino acid sequence of VIA from different areas was compared on the basis of the database in Genebank with soft-ware ClustalW1.83.The common epitopes which were able to bind strongly to MHCⅠ molecule were selected.Results:Though homologous degree of amino acid sequence of VIA from different areas was not completely similar,there were some common epitopes among these strains analyzed.The common epitopes were shown to bind strongly with MHCⅠ molecule.Conclusion:These common epitopes may be used to develope epitope-bacterin for preventing infection of AIV(H5N1).

Objective To explore the clinical efficacy and safety of ropivacaine for combined spinal-epidural anesthesia(CSE).Methods 40 patients,who intended to undergo elective lower limb or perineum surgery,were randomly divided into two groups,each with 20 cases,ropivacaine were used in treatment group,while bupivacaine in control group the differences of feeling,motor block and recovery time,as well as their side effect in the two groups were compared.Results The maximum of spread time of anesthesia and the recovery time,and side effect in treatment group were similar to those in control group.The onset time of motor block and the peak time of motor block were longer in treatment group than that in control group,the maximum modified Bromage scale in treatment group was lower than that in control group(P

Proteio degradation via ubiquitin-proteasome system(UPS) is carried out with a wide variety of signallingpathways,from cell cycle and transcription to development.The mass spectrometry-based proteomics techniques are powerful tools in the research of UPS.The recent advance in the study of UPS by proteomics techniques is briefly reviewed.