Objective: In order to establish an effectively analytic method about the content of lead, arsenic, cadmium, mercury and copper. Methods:According to the standard addition method, matrix modifiers (nickle nitrate and diammonium orthphosphate) were added to sample and the contents of Pb, As, Cd and Cu were determined by graphite furnace atomic absorption spectrometry, reduce agent (acid tin bichloride was added to sample and the content of Hg was determined by cold vapour atomic absorption method. Results: The average recoveries of lead arsenic, cadmium, copper and mercury were Pb=97.5%, RSD = 2.75%; As=96.6%, RSD = 2.23%; Cd= 98.2% , RSD = 1.32% ;Cu=97.8%, RSD =1.19%;Hg= 98.8% , RSD = 1.85 , respectively. Conclusion: The method is sensible, accurate and reproducible. and applied for quality control of content of heavy metals in Qijidehuang Pills.

ObjectiveTo investigate the effect of urinary kallidinogenase on the expression of Bcl-2 and Bax in cerebral ischemia-reperfusion injury of rats.Methods Forty eight male adult Sprague-Dawley rats were randomly assigned into four groups: sham operation group, model group,normal saline group andurinary kallidinogenase group.The middle cerebral artery occlusion reperfusion model was made by the suture method. After focal cerebral ischemia-reperfusion, the animals were neurologically assessed on a 5 point scale. The levels of Bcl-2 and Bax expression were measured by immunohistochemical and RT-PCR.Results In model and normal saline group, the expressions of positive Bcl-2 neurocytes and mRNA [(14.28±2.54)/HP, 0.551±0.068 and (16.54± 1.84)/HP, 0.585 ± 0.084] were significantly increased compared with the sham operation group [ (7.58 ± 0.97 )/HP、 0.324 ± 0.042] ( P ＜ 0.05 ) ; The expressions of Bax positive neurocytes and mRNA[( 24.38 ± 3.58 )/HP, 0.540±0.076 and (26.74 ±4.04) /HP, 0.527 ± 0.065] were significantly increased than the sham operation group [ (8.24±1.95 )个/HP, 0.309 ± 0.037] (P ＜0.05). After treatment with urinary kallidinogenase, the expressions of Bcl-2 positive neurocytes and mRNA [(25.61±3.41)/HP, 0.791 ±0.096] were upregulated ( P＜0.05), and the expressions of Bax positive neurocytes and mRNA [( 18.54 ± 2.38)/HP, 0.359±0.053] were down regulated (P＜0.05), compared with model group and normal saline group.ConclusionsUrinary kallidinogenase is significantly related to the upregulation of Bcl-2 expression and the downregulation of Bax expression, which suggest that urinary kallidinogenase could be related with the inhibitory effects on ischemic neurocyte apoptosis.

Objective To investigate the antimicrobial and antitumor activities of extracts from the root of mangrove plant,Kandelia candel.Methods Micro-dilution and MTT method were used to test the antimicrobial and antitumor activities of extracts from the root of K.candel.Results The petroleum ether extracts of root could inhibit the growth of Staphylococcus aureus,S.epidermidis,Bacillus subtilis and Sarcina lutea with the minimal inhibitory concentration(MIC) of 3.125 mg?mL-1,1.5625 mg?mL-1,0.78125 mg?mL-1 and 3.125 mg?mL-1 respectively.The ethyl acetate extracts and ethyl alcohol extracts could inhibit the growth of S.epidermidis with MIC of 3.13 mg?mL-1 and 6.25 mg?mL-1 respectively.At the same time,the petroleum ether extracts could inhibit the proliferation of tumor cells,such as SMMC-7721,B16,BGC803 and HepG2 with IC50 of 355.2?g?mL-1,378.6?g?mL-1,275.6?g?mL-1 and 411.6?g?mL-1 respectively,while the other extracts could stimulate the proliferation of tumor cells apparently.Conclusion The antimicrobial and antitumor activities of extracts from the root of K.candel indicate the potential pharmaceutical usage of this plant.

Objective To develop a method for detecting anti-cyclic citrullinated peptide (anti-CCP)in the serum of rheumatoid arthritis patients. Methods The range of lineage correlation, precision and detection range of time-resolved fluoroimmunoassay (TRFIA) was analyzed. Thirty-two positive serum of antiCCP, and the sera from 50 health blood donors, 32 SLE patients, 26 patients with SS, 10 patients with scleroderma, 20 patients with MCTD and 18 patients with MS were tested in this study. The clinical specificity was assessed. The consistency between TRFIA and ELISA was analyzed by Mc Nemar test. Results Analyzed by SPSS 13.0 statistical software, the intra-batch precision (n=20) rate was 2%, 3% and 4% respectively, and the inter-batch precision (n=8) was 3%, 4% and 7% respectively for 3 different concentrations. The clinical specificity was 98%, 97%, 96%, 100%, 95% and 100% in the sera of healthy blood donors, SL,E, SS,scleroderma, MCTD and MS patients respectively. The correlation coefficient was 0.989.The average recovery rate was 101%, and the sensitivity was 1 U/ml. When compared with ELISA, the detection range of TRFIA was wider and also with betterstability. Conclusion TRFIA is a stable method with high sensitivity and wide detection range. It can be used to detect anti-CCP antibody. It is important for early diagnosis of RA and monitor the curative effect of RA. And this method has potential to be used in clinical diagnosis.

Objective To evaluate global and segmental right ventricular ( RV ) systolic functions in patients with excessive volume or pressure load using real‐time three‐dimensional echocardiography ( RT‐3DE) . Methods Forty‐five patients with RV volume overload ,45 patients with RV pressure overload and 45 healthy subjects were underwent RT‐3DE . RV global and segmental ( inflow ,body ,outflow ) end‐diastolic volume (EDV) ,end‐systolic volume (ESV) ,stroke volume (SV) and ejection fraction (EF) were analyzed with TomTec software . The correlations between EF with the three‐dimensional method and two‐dimensional parameters including right ventricle systolic pressure( RVSP) were discussed . Results Global EDV and ESV increased significantly in both patient groups compared with controls ( all P < 0 .05) ,but there was no difference between two patient groups ( P >0 .05) .Compensated increase of SV was found in sixty percent of patients with volume overload but none with pressure overload ( P < 0 .05) . Global EF decreased significantly in both of patient groups (all P <0 .05) ,which was more significant patients with pressure overload ( P < 0 .05 ) . Different patterns of the regional dysfunction were found among the different RV segments . No correlation was found between RVSP and global or segmental EF in patients with pressure overload . Conclusions RT‐3DE could be used to assess global and segmental RV systolic function in patients with pressure and volume overload .

Objective To evaluate the geometry of mitral valve and annulus in patients with mitral regurgitation, and to quantify the mitral annular remodeling and motion in ischemic mitral regurgitation (IMR) with mitral valve assessment (MVA) software. Methods Seventeen patients were divided into two groups: IMR group (n=10) and non-IMR group (n=7), and other 11 patients without significant mitral regurgitation (＜2+) and mitral structure abnormality were regarded as control group. The imaging was analyzed offline with Siemens MVA software to measure the annular diameters, annular circumference, anterior and posterior annular length, annular area (MAA) and non-planar angel (NPA). The dynamic changes during the cardiac cycle were calculated. Results Compared with control group, the posterior annular length and NPA significantly increased in IMR group (P＜0.05, P＜0.01). During the systole, the posterior annular and annular circumference paradoxically prolonged in IMR group compared with control group (median Δ posterior annular length: -2.71 mm/m2 vs 0.52 mm/m2, P＜0.05). In all patients of non-IMR group, the geometric character of mitral valve lesions shown in 3-dimensional echocardiography was accordant with surgical findings. Conclusion Three-dimensional echocardiography combined with MVA software provides a useful tool for assessment the geometric character of mitral valve lesions and quantification of mitral annulus remodeling, which is helpful to understand the mechanism of MR and guide surgical treatment.

Objective To observe the clinic efficacy of open transforaminal lumbar interbody fusion (TLIF) compared with minimally invasive operation in treating lumbar spinal stenosis and instability among obese and non‐obese patients .Methods A ret‐rospective analysis was performed in these cases of mono‐segmental lumbar spinal stenosis and instability between January 2011 and January 2013 .Perioperative index ,clinical efficacy ,and imaging results were observed and compared between different groups .Re‐sults Thirty‐four obese cases and 105 non‐obese cases were divided into two groups ,including conventional posterior open TLIF and minimally invasive TLIF operation ,to compare the results .Perioperative indexes of obese patients were more than non‐obese patients undergone open TLIF operation way and there was significant difference(P<0 .01);while by minimally invasiveTLIF oper‐ation way ,comparing the results of the surgical blood loss and incision size between obese and non‐obese group ,there was signifi‐cant difference(P<0 .05) .However ,there was not significant difference in the operative time between two groups(P>0 .05) .No cases of slippage or breakage of implants were found among all these patients after 6 months of follow up .Postoperative VAS and ODI among these four groups were better than before(P<0 .01) .Undergoing open postoperative VAS in obese group and in non‐obese group ,there was significant difference(P< 0 .05);undergoing minimally invasive postoperative ODI in obese group and in non‐obese group ,there was significant difference(P< 0 .05) .But ,undergoing open postoperative ODI in obese group and in non‐obese group ,there was not significant difference(P>0 .05);undergoing minimally invasive postoperative VAS in obese group and in non‐obese group ,there was not significant difference(P>0 .05) .Conclusion Therefore ,obese may be risk factor in treating lumbar spinal stenosis and instability .

Objective To investigate the correlation between NOTCH3 polymorphic locus rs1043994 and white matter lesions (WML). Methods The enrolled subjects were elderly in the outpatient clinic for health check-up from January 2015 to January 2017. According to the results of cranial MR examination, 337 elderly people were divided into the WML group (n=172) and normal control group (n=165). The inclusion criteria were: (1) age ≥ 50 years old; (2) those who can cooperate with head MRI examination; (3) those who understand the study and agree to retain blood samples for SNP testing. Exclusion criteria were: (1) previous neurological diseases such as cerebrovascular disease, intracranial infection, dementia, and trauma; (2) having a history of mental illness; (3) suffering from serious diseases such as liver and kidney dysfunction, heart disease, tumors. The clinical data of the subjects were collected and the peripheral venous blood was extracted for DNA extraction. The cognitive function was evaluated by the Mini-mental State Examination. The genotyping of the subjects was carried out by restriction endonuclease. The correlation between rs1043994 polymorphism and WML was analyzed by Logistic regression. Results There was no significant difference in gender, education level, diabetes, hyperlipidemia, smoking, uric acid and Hcy between the two groups (P>0.05). Compared with the control group, the WML group had a higher average age and a higher proportion of hypertension (P<0.05), and the Mini-mental State Examination scores between the two groups were statistically significant different (P<0.01). The genotypes (AA, AG, GG) frequency and allele (A, G) frequency distribution of rs1043994 were statistically different between the two groups (P<0.05). Multivariate Logistic regression analysis showed that age (P=0.001), hypertension (P=0.012) and AA genotype (P=0.019) were independent risk factors of WML (P<0.05). The risk of WML in AA genotype is 2.512 times higher than that in AG/GG genotype. Conclusions The rs1043994 polymorphism of NOTCH3 gene is associated with WML in the elderly population, and the A allele is a susceptibility gene for WML. The rs1043994 polymorphism of the NOTCH3 gene may be a genetic risk factor for WML in the Chinese elderly population.

Background and objective Lung cancer is one of the most serious disease and the incidence of non-small cell lung cancer (NSCLC) is the highest in lung cancer. The main reason for the failure of chemotherapy is the tolerance to cisplatin. Transcriptional regulator SOX4 plays an important role in the occurrence and development of many tumors, and regulates Wnt signaling pathway by regulating the expression of β-catenin. We aimed to investigate the role of SOX4 on cisplat-in-resistance in NSCLC cell A549 cell. Methods The cisplatin-resistance lung cancer cell line A549/DDP was constructed by induction method in vitro, and cisplatin-resistance detected by CCK8 assay. Growth curves of A549 and A549/DDP was cal-culated. The expression level of SOX4 in A549 and A549/DDP cells were detected by Western blot. A549/DDP were knock-down of SOX4 by siRNA transfection, and the cisplatin-resistance of detected by CCK-8 assay, the expression level of β-catenin and Survivin were detected by real-time PCR and Western blot. Results The cisplatin-resistance cell line A549/DDP was constructed successfully, and its cisplatin-resistance is 13.7 times higher than in A549. There was no significance difference between A549 and A549/DDP in cell proliferation. The expression level of SOX4 is higher in A549/DDP than in A549. The cisplatin-resistance significantly decreased in A549/DDP cells after knockdown of SOX4 by siRNA transfection. The expres-sion level of β-catenin and Survivin significantly decreased in A549/DDP cells after knockdown of SOX4. Conclusion SOX4 can strengthen cisplatin-resistance of non-small cell lung cancer cell A549.

Background and objective It has been proven that chlorogenic acids can produce anticancer effects by regulating cell cycle, inducing apoptosis, inhibiting cell growth, Notch signaling pathways are closely related to many human tumors. The aim of this study is to study the mechanism of chlorogenic acid on apoptosis of non-small lung cancer through Notch1 pathway in animal level, and hope to provide theory basis on clinical treatment and research aimed at targeting Notch1 signaling in non-small cell carcinoma (NSCLC).Methods MTT assay was used to evaluate the A549 cell proliferation under the treatment of chlorogenic acid. The effect of chlorogenic acid on apop-totic and cell cycle were detected by flow cytometry. The animal model of A549 cell transplanted in nude was estab-lished, tumer size and weight were detected. The mRNA level of Notch1 signal pathway related facter were detected by RT-PCR; the expression of Notch1 signal pathway related facter in tumor tissue was detected by western blot.Results Chlorogenic acid inhibited the A549 cell proliferation. incresed cell apoptotic and cell percentagein G2/M (P<0.05), and in a dose-dependent manner. In animal model, tumer size and weight were lower than control group, the difference was statistically significant (P<0.05). The relative expression of mRNA of Notch1, VEGF, Delta4, HES1 and HEY1 were decreaced (P<0.05) in tumor tissue which treated with chlorogenic. The expression of Notch1 were decreaced, PTEN, p-PTEN, p-AKT were increced significantly in tumor tissue which treated with chlorogenic (P<0.05).Con-clusion Chlorogenic acid can regulate theapoptosis of non-small lung cancer through Notch pathway in animal level,which may be associated with the down-regulating the expression of VEGF and Delta4. Notch pathway may cross talk with PI3K/AKT pathway through PTEN in NSCLC.