In the treatment of asthma, there are the commonly-used external therapies of scarring moxibustion, ginger-insulated moxibustion and acupoint herb-applying method, etc, with better clinical effects. But, strict recruitment standard was lacked and the designs of clinical studies were not complete in some reports. Therefore, the design of clinical study on moxibustion therapy and acupoint herb-applying method in the treatment of asthma needs to be strict and complete in the future studies.

Humans ; Laryngeal Diseases ; surgery ; Larynx ; surgery ; Microsurgery ; instrumentation ; Otorhinolaryngologic Surgical Procedures ; instrumentation ; Surgical Instruments

Objective: To quantify the effect of motor-developmental Tuina therapy on 61children with cerebral palsy. Methods: All the children received the motor-developmental Tuina therapy for 20 min each time, 3 to 6 times per week. The gross motor functions of the children were evaluated by GMFM 66. The baseline period evaluations in 15 children and follow-up period evaluations in 26 children were performed. Results: GMFM 66 score significantly increased at the end of treatment period (P<0.001). The GMFM 66 score changes per month in treatment period was significantly greater than those of both baseline period and follow-up period (P<0.001). Conclusion: The effect of motor-developmental Tuina therapy was notable on children with cerebral palsy. Continuous progress of the gross motor functions in these children may be obtained by continuous therapy.

Objective:To compare the biomechanical properties of different circular external fixators for the fracture site of oblique long bone fractures.Methods:15 polyethylene (PE) plastic rods with the same batch were selected to make the model of oblique fractures in the middle of long bones. According to the connection between PE rods and external fixators, the PE rods were randomly divided into: the group that was simply used kirschner wires (group kirschner wires), the group that was simply used olive wires (group olive wires), the group that was simply inserted half pins (group half pins), the group that was inserted single cortical fixed half pins at either side of the fracture site after being fixed by the tensioned olive wires (group olive wires+ half pin), the group that was inserted tensioned trans-fracture olive wires after being fixed by the tensioned olive wires (group olive wires+ olive wires). The axial compression tests were carried out, and the interfragmentary displacements under axial loads of 200 N, 400 N, 600 N and 800 N were measured and the axial stiffness was calculated. Then, the torsional tests were carried out, and the interfragmentary torsional angles were measured under torsional loads of 4 N·m, 7 N·m and 10 N·m, and the torsional stiffness was calculated.Results:With the increase of axial load from 200 N to 800 N, the axial interfragmentary displacement in each group gradually increased. The interfragmentary axial displacement of each group in ascending order was: group olive wires+ olive wires, group olive wires+ half pins, group half pins, group olive wires, group kirschner wires. The axial stiffness of each configuration under 800 N axial load in descending order was: group olive wires+ olive wires [863.93 (824.32, 875.87) N/mm], group olive wires+ half pins [119.92 (113.16, 123.58) N/mm], group half pins [81.92(79.42, 82.40) N/mm], group olive wires [76.83 (72.45,79.47) N/mm], group kirschner wires [70.80 (67.49, 71.59) N/mm]. The pairwise comparisons of the axial stiffness data of each configuration had statistical significance (all P <0.05). With the increase of the torque load from 4 N·m to 10 N·m, the interfragmentary rotational angle in each configuration gradually increased. The interfragmentary torsion angle of each group in ascending order was: group half pins, group olive wires+ olive wires, group olive wires+ half pins, group olive wires, group kirschner wires. The torsional rigidity of each configuration under 10 N·m torsional load in descending order was: group half pins [1.80 (1.63, 1.85) N·m/°], group olive wires+ olive wires [1.05 (1.02, 1.07) N·m/°], group olive wires+ half pins [0.99 (0.98, 1.03) N·m/°], group olive wires [0.81 (0.78, 0.82) N·m/°], group kirschner wires [0.75 (0.74, 0.76) N·m/°]. The pairwise comparisons of the torsional rigidity data of each configuration had statistical significance (all P < 0.05). Conclusion:The axial stiffness and torsional stiffness of circular external fixators can be increased by using tensioned olive wires or half pins at the fracture site. Due to the insufficient support between oblique fracture site, when the load is applied, the axial displacement and torsion angle of the fracture site will still be fairly large after being fixed the fracture site with half pins. Treating with tensioned trans-fracture olive wires after being fixed by the tensioned olive wires at either side of the fracture site can effectively control the interfragmentary shear and displacement, thus providing an ideal mechanical environment for fracture healing.

Objective : To explore the effect of FTO(fat mass and obesity⁃associated protein) and inhibitor on rituximab resistance in DLBCL(diffuse large B cell lymphoma) . Methods : Two cell lines derived from “ double strike ” lymphoma were selected , activated B ⁃cell⁃like ( ABC) OCI⁃LY8 ( LY8) and germinal center B ⁃cell⁃like ( GCB) OCI⁃LY18 (LY18) , and rituximab⁃resistant DLBCL cell lines (LY8R and LY18R) were constructed by “ concentration gradient dosing method ” . The CCK⁃8 method was used to detect the cell survival rate of different concentrations of rituximab after interfering with the parental and drug⁃resistant cells , and the half maximal inhibitory concentration(IC50 ) was calculated ; AnnexinⅤ/PI double staining method was used to detect the apoptotic rate of parent cells and drug⁃resistant cells ; qRT⁃RCR , immunofluorescence and Western blot were used to detect the expression of demethylase FTO in rituximab⁃resistant cells . The expression of FTO was inhibited by adding meclofenamic acid (MA) to drug⁃resistant cell lines , and the inhibition was verified by RT⁃qRCR and Western blot . The expression of c⁃Myc and CEBPA in drug⁃resistant strains after inhibiting FTO was detected by using Western blot . Results: Compared with the parent cells , the expression of demethylase FTO and c⁃Myc increased , and the expression of CEBPA decreased in the rituximab⁃resistant cell lines , with statistical significance (P < 0. 01) . After the drug⁃resistant cell lines treated with MA , the expression of FTO and c⁃Myc decreased and CEBPA increased , all with statistically significant differences (P < 0. 01) . Conclusion The expression of FTO in drug⁃resistant DLBCL significantly increases , and MA inhibits the expression of FTO in drug⁃resistant cells , which may have therapeutic potential for rituximab⁃resistant DLBCL.

Rho-associated kinases (ROCKs) are serine-threonine protein kinases that act downstream of small Rho GTPases to regulate the dynamics of the actin cytoskeleton. Two ROCK isoforms (ROCK1 and ROCK2) are expressed in the mammalian central nervous system. Although ROCK activity has been implicated in synapse formation, whether the distinct ROCK isoforms have different roles in synapse formation and function in vivo is not clear. Here, we used a genetic approach to address this long-standing question. Both Rock1 and Rock2 mice had impaired glutamatergic transmission, reduced spine density, and fewer excitatory synapses in hippocampal CA1 pyramidal neurons. In addition, both Rock1 and Rock2 mice showed deficits in long-term potentiation at hippocampal CA1 synapses and were impaired in spatial learning and memory based on the water maze and contextual fear conditioning tests. However, the spine morphology of CA1 pyramidal neurons was altered only in Rock2 but not Rock1 mice. In this study we compared the roles of ROCK1 and ROCK2 in synapse formation and function in vivo for the first time. Our results provide a better understanding of the functions of distinct ROCK isoforms in synapse formation and function.