[Objective] To observe the effect of serum containing Bushen prescription (BP) on inhibition of chondrocyte proliferation and nitric oxide (NO) production in chondrocyte induced by interleukin-1 (IL-1). [Methods] Four New Zealand rabbits were administered BP 2.5mL (1.15kg/L) for 3 days for the preparation of BP-containing serum. Rabbit chondrocytes were cultured in-vitro and were added with DMEM culture containing IL-1 1, 10 and 20 ?g/L to examined the inhibition concentration of IL-1 for chondrocyte proliferation. After then, BP group was added with 20 ?g/L IL-1 and various concentrations of BP-containing serum and the model group with 20 ?g/L IL-1 and various concentrations of blank serum. Alter 3-day culture, chondrocyte proliferation were dectected by thiazolyl blue colorimetry (MTT) and NO content by Griess method. [Results] The inhibition concentration of IL-1 for chondrocyte proliferation was 20 ?g/L; various concentrations of BP-containing serum could counteract chondrocyte proliferation and inhibit NO pnxluction in chondrocyte induced by IL-1. [Conclusion] BP can counteract chondrocyte proliferation directly or indirectly by the mediation of NO, which may be one of its therapeutic mechanisms in treating osteoarthritis.

Objective To evaluate the effect of multiple adhesion molecules ICAM-1、CD_ 44 V6、CD_ 28 、E-cad and CEA in organic selection of gastric carcinomatous metastasis.Methods Eighty-four gastric carcinoma cases(32 cases without metastases,52 cases with transfer to liver、marrow、lymphonode、lung、kidney or belly cavity),were take operation sample or tissue under gastroscopy,being made cells single by primary culture,then were detected the expression of 5 kinds of adhesion molecules by ABC immunohistochemistry,positive rate was recorded.Results ICAM-1 had high expression in cases with marrow or lung metastasis,while low expression in cases with lymphonode metastasis.CD_ 44 v6 had high expression in cases with lymphnode or liver metastasis.CD_ 28 had high expression in cases with liver、lung or kidney metastasis.E-cad had high expression in cases with marrow metastasis,while low expression in cases with other metastasis.CEA had especially high expression in cases with liver、belly cavity or lymphnode metastasis.Conclusion High or low expression of adhesion molecules ICAM-1、CD_ 44 V6、CD_ 28 、E-cad and CEA plays a certain role in organic selection of gastric carcinomatous metastasis.

Objective To evaluate the role of 4 types of adhesion molecules in digestive system tumors with bone marrow metastasis.Methods The fresh bone marrow slies of 72 cases of digestive system tumor with bone marrow metastasis,including 26 gastric cancer,25 liver cancer,21 colorectal cancer,were obtained.The tumor cell slides of 76 cases of digestive system tumor without metastasis,including 32 gastric cancer,20 liver cancer,24 colorectal cancer,were obtained.The expressions of 4 kinds of adhesion molecules in two groups were studied by ABC immunohistochemical methods,and positive rate was recorded and accumulated points.Results ICAM-1、E-cad and CEA positive rates/CD44V6 accumulated points were up-regulated in gastric cancer cells in bone marrow;ICAM-1、CD44V6 accumulated points/CEA positive rate were up-regulated in liver cancer cells in bone marrow,while expression of E-cad had no difference;ICAM-1、CD44V6 and CEA accumulated points/E-cad positive rate were up-regulated in large bowel cancer cells in bone marrow.Conclusion Different adhesion molecules play coordinative or resistant role in digestive system tumor with bone marrow metastasis.

Objective To investigate the retinoic acid incubation effect on proliferation of U251 cell line and effect on MAPK signal pathway. Methods ATRA solution of different concentration on the U25 1 glioma cells were incubated,the influence of ATRA on the proliferation of U25 1 cells were detected,and the proteins of MKPs and MAPK signaling pathways were detected by qRT-PCR and Western blot.Using Graph Prism 5 software for quantitative analysis of experimental results.Results Compared with control group,ATRA could effectively inhibit the proliferation of U25 1 glioma cells, in a concentration dependent manner.QRT-PCR results showed that,different concentrations of ATRA after incubation for 48 hours,the expression of MKPs mRNA changed,but the changes of MKP-5 and expression of 67LR was different,explained the main differences between the two methods of the MAPK signaling pathway was the regulation of MKP-5.Western blot results showed that the ATRA,after 48 hours of incubation,the protein MAPK pathway had changed in phosphorylation, which showed that ATRA protein in the MAPK signaling pathway through control of the degree of phosphorylation on U25 1 cell line regulation.Conclusion Retinoic acid and retinoic acid receptor play its physiological effects and regulate human glioma cell line U25 1 proliferation through different combination.Retinoic acid could not only reduce the expression of phosphorylated ERK1/2 to inhibit tumor proliferation,but also regulate three kinds of protein phosphorylation,therefore its mechanism will be more complex,at the same time that the MAPK signaling pathway plays a crucial role in tumor proliferation process.

Objective To investigate the significance of bone marrow cell morphology on clinical diagnosis of children hematopoietic diseases.Methods The data of bone marrow cell morphology in the bone marrow puncture specimens from 4 590 children admitted to Children' s Hospital of Shanxi Province from January 2009 to December 2014 were retrospectively analyzed.Results The proportion of infancy patients was the highest in 4 590 bone marrow specimens, accounting for 29.0 ％ (1 333/4 590), then that of the toddler age patients was second highest, accounting for 26.7 ％ (1 224/4 590).The constituent ratio of thrombocytopenic purpura (ITP) at the different ages was the highest.The most common diseases in bone marrow cell morphology diagnosis were in order of ITP, iron deficiency anemia (IDA), infectious bone marrow, leukemia (acute lymphoblastic leukemia, acute myeloid leukemia), aplastic anemia and so on.Conclusions Accurate analysis of bone marrow cell morphology is still the most basic and rapid approach in children with hematopoietic system disease, which has important value.Except hematopoietic system diseases, once fever of unknown origin, hepatosplenomegaly and enlargement of lymph nodes the patients should be early given bone marrow cell morphology check, early diagnosis and therapy.

Objective To explore a method to improve the nutritional state and immune function of patients after radical gastrectomy,and to decrease postoperative complication rate and accelerate recovery from operation.Methods In line with the concept of fast tract surgery (FTS),a new perioperative nutritional schedule was made for patients prior to radical gastrectomy,ehich included preoperative nutritional risk screen and prophylactic enteral nutrition,early postoperative light enteral nutrition,early ambulation and perioperative nursing care.Patients with gastric cancer were randomly assigned into fast track group or a simple control group.New perioperative schedule was executed in the patients of fast track group and traditional nutrition support in control group.After operation,serum albumen was measured to evaluate the patient's nutritional state and serum IgA to evaluate gastrointestinal immune function.In addition,the time to passage of flatus,postoperative recovery time and complication rate after operation were compared.Results Patiens in FTS group (n=35) showed higher serum albumin and serum IgA on d1 and d3 after operation than those in the control group (n=25) (P

Objective Intestinal ischemia-reperfusion (I-R) is a critical and triggering event in the development of distal organ dysfunction,frequently involving the lungs.In this study we investigated the effects of edaravone on the prevention of lung injury induced by intestinal I-R in rats.Methods Eighteen male SD rats were randomly divided into 3 groups: Sham operation group (group Sham),I-R group (group IR) and edaravone group (group E).After injecting 6 mg of edaravone or the same volume of 0.9% sodium chloride,the anterior mesenteric artery was clamped with a noninvasive microvascular clip for 120 min and then reperfusion for 120 min.Sham-operated animals underwent the same surgical procedures without clamping.Lung tissues were collected for pathology tested by HE dyed,blood as collected for the analysis of TNF-α and IL-6 concentration by ELISA,small lung tissues were collected for the analysis of lung myeloperoxidase (MPO) activity and malonialdehyde (MDA) concentration.Results Compared with group Sham,the alveolar epithelial cells in group IR was widespread edema,inflammatory cell infiltration,atectasis,disruption of alveolar,and pulmonary capillary hemorrhage.The pathological changes of lung tissue in group E were significantly improved compared with those in group IR,and that of alveolar inflammatory exudate was decreased.Pathological scoring of group E (2.1±0.7),which was significantly lower than that of group IR (5.7±1.1) and group sham (1.5±0.2) score (P<0.05),so the concentration of TNF-α,IL-6,MPO activity and MDA concentration of group E were less than those of group IR (P<0.01).Conclusion Edaravone ameliorated the lung injury induced by intestinal I-R.

Objective:To analyze and optimize the extraction technology of Geum aleppicum by Box-Behnken design and response surface methodology. Methods:With the content of total flavonoids as the index, the effects of three factors including the solvent-solid ratio, ethanol concentration and extraction time on the content of total flavonoids were studied by single factor experiment, and the ex-traction technology was optimized by Box-Behnken design and response surface methodology. Results: The optimal extraction condi-tions of Geum aleppicum were as follows:the solvent-solid ratio was 4. 2, the ethanol concentration was 50% and the extraction time was 80 min. Under the above conditions, the average yield was 12. 590 0 mg·g-1 . Conclusion: Optimizing extraction technology of the total flavonoids in Geum aleppicum by Box-Behnken design and response surface methodology is reasonable, and the mathematical mod-el is consistent with the experimental data, which has good predictability.

Objective To investigate the surgical outcome of cervical spondylotic myelopathy. Methods Twenty -five patients (14 males and 11 females) with cervical spondylotic myelopathy who underwent operative treatment between 2004 and 2007 in our hospital were included in this study. The mean duration of symptoms was 31 months (ranging from 8 days to 10 years) . The follow - up period ranged from 18 months to 4 years (mean 30 months). The study comprised 6 posterior decompression (laminoplasty) and 17 anterior cervical discectomy with fusion and anterior locking plate systems. 2 patients with severe cervical spondylotic myelopathy were treated by operation of the combining anterior and posterior. Results According to COA, the recovery (improving) rate among the tolal patients was classified as exellent in 20 cases, good in 3 cases, fair in 2 cases. The excellend and good rate was 92% . Conculusion Both anterior and posterior surgery were effective for cervical spondylotic myelopathy with effective decompression according to the location of abnormality.

Objective To detect specific IgM of Lyme disease indirect ELISA using recombinant outer surface protein C(OpsC)of Borrelia burgdorferi in vitro was established. Methods Coated concentration of recombinant OspC and dilution multiple of serum anol concentration of enzyme secondary antibody were determined by block,and degree of percision.specificity interference and interruption test were performed. Results Best concentration of OspC was 150 μg/L.within-run CV was 6.3% between-run CV was 11.8%.Confimred 33 Lyme patients in clinic and 57 controls were examined meanwhile by this method and import ELISA kit,coincidena rate was 97.8%.Conclusion This ELISA using recombinant OspC was a good method for early diagnostic of Lyme disease.