Objective: To investigate the problems and improve the quality of experimental teaching for medical laboratory instruments.Method: A questionnaire was made among 100 laboratory medicine students in Chongqing University of Medical Sciences.Result: About eighty-six percent of the students were satisfied with the experimental teaching for medical laboratory instruments,but there were still problems: absence of clear objectives in experimental teaching;shortage of activity of medical laboratory instruments experiment;lack of modern large-scale instruments.Conclusion: the key points to improve experimental teaching quality are to set up clear experimental teaching objective;update instruments for experimental teaching;structure simulator laboratory with modern education simulator technology.

Students will be familiar with the major contents of clinical laboratory management,such as clinical laboratory quality management,laboratory creature safety containment,clinical laboratory accredit action through the course of clinical laboratory management for laboratory medicine students.Meanwhile,by continuously perfecting the teaching contents and methods,students' satisfaction with this course has been improved,and they pay more attention and give higher appraisement to this course.

Objective To appraise the values of combined determination of cTnI,MYO and CK-MB mass in the diagnosis of viral myocarditis.Methods The myocardial injury markers(cTnI,MYO and CK-MB mass)and myocardial enzyme(LDH,AST,CK and ?-HBDH)of 61 patients with viral myocarditis(VMC),74 patients with non-viral myocarditis(NVMC)and 46 healthy subjects were determined,respectively,by microparticle chemiluminescent immunoassay and continuous monitoring assay.Diagnostic efficiency was calculated by matrix decision method.Results The serum levels of cTnI,MYO and CK-MB mass of the patients in VMC group were obviously higher than that of the patients in NVMC group(0.46?0.21 vs 0.06?0.04?g/L,98.7?38.2 vs 39.2?26.8?g/L and 6.1?4.2 vs 2.2?1.7?g/L,respectively)as well as that of healthy subjects(0.46?0.21 vs 0.07?0.04?g/L,98.7?38.2 vs 36.5?24.7?g/L and 6.1?4.2 vs 2.1?1.5?g/L,respectively,P0.05).For preliminary diagnosis of VMC,the SE of MYO is highest,but the SP of cTnI and CK-MB mass were better choice.Restoration of MYO was earliest while cTnI was latest during convalescence of the patients.Conclusion Combined and uninterrupted determination of the myocardial injury markers can provide better diagnostic accuracy.

Rough set is a mathematical approach to study imprecise, uncertain or incomplete information or knowledge. The data mining technology based on the rough set theory can be trained through the training data set. The technology is applied to such fields as the prognosis of the pain in the chest, emergence diagnosis in ICU, distinguishing and diagnosis of lung tumor and rheumatoid disease, diagnosis of galactophore disease, diagnosis of premature birth, classification and diagnosis of liver disease and acute appendicitis. The technology has a brilliant future in medical data mining.

AIM: To demonstrate the changes of activity and electron microscopic enzyme cytochemistry staining of H+-K+-ATPase of gastric parietal cells under stress in rats.METHODS: Twenty-four male SD rats were randomly divided into normal group,stress group and stress+omeprazole(OM) group.Water immersion-restraint stress(WRS) model in SD rats was performed.The ulcer index(UI) of gastric mucosa and H+-K+-ATPase activity of gastric parietal cells were measured.The changes of ultrastructure and electron microscopic enzyme cytochemistry staining of parietal cells were observed under transmission electron microscope(TEM).RESULTS: Compared with control group,the UI of gastric mucosa and H+-K+-ATPase activity of gastric parietal cells increased(P

Clinical testing of equipment is an interdisciplinary professional and technical course,of which we made continuous innovation and practice by optimizing the curriculum system ,upgrading teaching contents,reforming teaching methods,opening up the construction of teach-ing materials,building the teaching staff and improving teaching quality management etc.After five years of positive efforts the remarkable teaching results were achieved.

By updating their concepts of education,carrying out the reform of teaching content,teaching methods and means,strengthening the construction of teaching materials,creating a high standard teaching team,the author aims to implement the construction of quality course of clinical testing equipment and comprehensively improve the teaching quality.

Objective To study the biological effect of different dose of satereotactic body radiation therapy (SBRT) on hepatoma Hep G2 Cells.Methods Hep G2 hepatumor cells vitality of 0,2,4,6,8,and 10 Gy doses of SBRT were determined by methyl thiazolyl tetrazolium (MTT) method.The cell colony formation rate and the cell survival fraction were detected.The cell apoptosis was detected by flow cytometry.The expressions of cyclin D1,Bax,Bcl-2,and caspase-3 protein were detected by Western blot.Results Hep G2 cells vitality was obvious inhibitory effect in different doses of SBRT,increasing with the SBRT dose,the cell vitality inhibition [2 Gy group (4.29 ± 0.63) ％,4 Gy group (19.37 ± 4.54) ％,6 Gy group (41.35 ±4.51)％,8 Gy group (86.34 ±5.68)％,10 Gy group (97.24 ±5.41)％] was increased,compared to 0 Gy group [(2.94 ± 0.52) ％],the difference was statistically significant (all P ＜ 0.01 or P ＜0.05).The cell colony formation rate and survival score in different doses of SBRT were statistically lower than the control group,increasing with the SBRT dose,the cell colony formation rate [4 Gy group (83.15 ± 4.71),6 Gy group (65.23 ± 5.47),8 Gy group (41.28 ± 5.36),10 Gy group (8.14 ± 5.22)] and survival score [2 Gy group (95.49 ±3.21),4 Gy group (84.23 ±3.45),6 Gy group (66.08 ± 3.71),8 Gy group (41.82 ± 3.82),10 Gy group (8.25 ± 3.56)] were decreased,compared to 0 Gy group [(98.72 ± 1.23),(100.00 ±0.00)],the differences were statistically significant (all P ＜0.01 or P ＜ 0.05).The cell apoptosis in different doses of SBRT was increased,increasing with the SBRT dose,the Hep G2 cells apoptosis [4 Gy group (13.67 ±4.62),6 Gy group (28.71 ±5.13),8 Gy group (54.16 ±5.24),10 Gy group (76.31 ± 5.17)] were significantly increased,compared to 0 Gy group (1.35 ±0.46),the difference was statistically significant (all P ＜ 0.01).The expressions of cyclinD1,Bax,Bcl-2,and caspase-3 protein in different doses of SBRT were decreased,increasing with the SBRT dose,the cyclin D1 [6 Gy group (1.94 ± 0.57),8 Gy group (1.38 ± 0.41),10 Gy group (0.52 ± 0.34)],Bax[6 Gy group (2.67 ±0.59),8 Gy group (1.73 ±0.45),10 Gy group (0.63 ±0.37)],Bcl-2 [4 Gy group (1.78 ±0.41),6 Gy group (1.03 ±0.35),8 Gy group (0.76 ±0.28),10 Gy group (0.38 ± 0.21)],Caspase-3 [6 Gy group (0.76 ± 0.31),8 Gy group (0.42 ± 0.24),and 10 Gy group (0.24 ± 0.21)] were obviously decreased,compared to 0 Gy group [(3.41 ± 0.75),(4.36 ± 0.87),(2.57 ± 0.46),(1.67 ± 0.55)],the difference was statistically significant (all P ＜ 0.05).Conclusions Different dose of SBRT can reduce Hep G2 cell vitality,enhance radiation sensitivity,have a dose dependent,maybe related to promote cell apoptosis and inhibit the proliferation of protein expression.

Objective To discuss the clinical features of trachea and bronchial tuberculosis and estimate the efficacy of the diagnosis of trachea and bronchial tuberculosis by fiberoptic bronchoscopy. Methods Clinical presentations and examination of fiberoptic bronchoscopy findings of 216 patients diagnosed by Olympus electric or fiberoptic bronchoscopy were retrospectively investigated. Results Male of the 216 patients were 95, female were 121, with 1.27 times higher incidence noted in female than in male subjects. An activator dry cough was the most complain in 72.7% , intermittent hemoptysis was in 34.7% , absenting of typical clinical poisoning symptoms of tuberculosis. Atelectasis and shape in lung were the most common chest roentgenographic presentations respectively in 31.0 % and 24.1 %. Chest roentgenographic presentations of 16.7% were normal. Bronchoscopic findings showed that main pathologic changes included 36.1% granulation, 31.0% mucosa inflammation, 24.1% ulceration (or necrosis) and 8. 8% cicatricial stenosis, left lung (56. 2%) was more often affected than right lung (37.6%), left bronchi (26.9% ) was in the first. The pathologic changes affected all of leaf, segment bronchi. One hundred and seventy-eight cases (82.4%) were diagnosed by bronchoscopic biopsy, 68 cases (31.5%) were diagnosed by bronchoscopic brushing examination for acid - fast bacillus. Conclusion The clinical features of trachea and bronchial tuberculosis are non - specific and easy to be misdiag-nosed. It is the main reason to be misdiagnosed that bronchial biopsy is neglected by clinical doctors. Bronchial biopsy should be the most reliable and accurate step to get the definite diagnosis.

A novel disposable three electrodes blood alcohol biosensor strip was fabricated by a screen printing technique. Multi-wall carbon nanotube(MWCNT), Meldola′s(MB), alcohol dehydrogenase(ADH)and nicotinamide adenine dinucleotide cofactor (NAD+) were modified on the surface of the carbon working electrode. Then hydrophilic membrane was stuck in the outermost of the three electrodes to make a reaction camera of 5 μL. Experimental results indicated that the biosensor possessed good accuracy and stability, the linear response range was 0.5-20 mmol/L with correlation coefficient of 0.9949, detection limit was 0.22 mmol/L, and the response time was less than 15 s. Some influencing factors to the biosensor were investigated, such as the pH, temperature and interferences. Correlation analysis showed that there was a significant correlation between the methods of biosensor and the headspace vapor phase chromatography in 10 whole blood samples(r=0.97583). Small volume whole blood sucked using siphonage to detect blood alcohol directly and quantitatively was the obvious character of the biosensor.