1.A Discussion on Anaesthetist's moral training
Chinese Medical Ethics 1995;0(02):-
Besides performing medical moral rules, aneasthetist should be of special morals and deal with the relation to patients and surgeon.
2.Epilepsy surgery in China: The history and current development
Neurology Asia 2007;12(Supplement 2):1-3
The earliest activities related to epilepsy surgery in China ma�� � y be tra�ce��d to the 19���50s an�� �� d 60s��. A�ter
a lull during the cultural revolution, there was resurgence of epilepsy surgery from the 1980s, and
rapid development from 2000. It is estimated that app�roximately 150- ���200 p hysi�cians �currentl�� � y pra�cti�ce
epilepsy surgery in the country.�� In year 2��� 005, there were a total of 2,��500 �cases of e�pile�ps��y o�perate��d in
whole of �China, close to half in Beijing, Tian�jin, �Shanghai, �Chongqing an��d Guang�zhou, an�� a �uarter
in Beijing. There are three Chinese epilepsy surgery journals, 5 monographs on epilepsy surgery, and
“��Epilepsy surgery” edite�� ��� ��� �� ��� ��� d by HO Luders has also �been translate��d into �Chinese��.
3.The pediatric epilepsy surgery in China
Neurology Asia 2007;12(Supplement 1):33-35
There is rapid growth of pediatric surgery service in China in the recent years. A survey by the China
Association �A gainst �Epi�lepsy underta�ken in �June ����� 2006���� on the ma�jor cities in different parts of the
country showed a dramatic increase particularly in years 2004 and 2006. Surgery for temporal lobe
epilepsy accounts for half of the operation. Surgery has an important role in the treatment of drug
resistant pediatric epilepsy. With large population and vast geographical spread, it is important to
further develop pediatric epilepsy surgery service in China
4.The transcription of interleukin-8 in gastric epithelial cells downregulated by ORF10 of cag Ⅱ of Helicobacter pylori
Shude LI ; Guoming XU ; Zhaoshen LI ;
Chinese Journal of Digestion 2001;0(03):-
Objective Helicobacter pylori (H. pylori )contains more than 30 genes and many of them in cag pathogenicity island (cag PAI) composed of cag Ⅰ and cag Ⅱ are involved in eliciting the transcription of interleikin 8 (IL 8) and the induction of IL 8 protein secretion in gastric epithelial cells, although some genes are not involved. This study was designed to observe the effect of ORF10 of H. pylori cag Ⅱ on the transcription of IL 8 in gastric epithelial cells and to test the possibility that certain genes in the cag PAI also downregulate (modulate) the transcription and expression of IL 8 in gastric epithelial cells. Methods Three strains of H. pylori mutants with deletion of ORF10 gene (ORF10, namely 28 1, 28 2, 28 3) and L5F11 cells containing IL 8 reporter gene were constructed. The constructed L5F11 cells were co cultured with the above strains and the luciferase activity (IL 8 transcription) was measured in a scintillation counter and the concentration of IL 8 protein was assayed by enzyme linked immunosorbent assay (ELISA). Results The activities of luciferase induced by 3 strains of H. pylori mutants with deletion of ORF10 gene were much higher than that of the mother strain 26695 [ 28 1:(1.49 ? 0.27)?10 6 cpm vs. (0.67 ? 0.08 )? 10 6 cpm, P
5.Study on the Resistance and the Resistant Plasmid Profiles in Prevalent Gonococcal Strains
Guoming LI ; Qun CHEN ; Shengwen SHAO
Chinese Journal of Dermatology 2001;34(3):192-194
Objective To investigate the resistant status and to type the phenotype and plasmid profiles of prevalent gonococcal strains. Methods A total of 98 gonococcal strains isolated from 1998 to 1999 in Zhanjiang was identified systemically. The susceptibilities of 98 strains of gonococci to penicillin,tetracycline and ciprofloxacin were detected by agar dilution method. The β - lactamase production was tested by iodometric method. The plasmids were extracted by alkaline lysis technique. Results The prevalence of gonococcal strains resistant to penicillin, tetracycline and ciprofloxacin were 22.44% ,45.94% and 55.12% ,respectively. There were 6 positive strains for β - lactamase, accounting for 6.12% (6/98). The most common phenotypes were susceptiblePT、 CMRNGT、 CMRNGPT and TRNG, accounting for 43.86% ,23.46% ,10.20% and 10.20% ,respectively. The detection rate of plasmids were 91.84% .Among them, the percentages of 42.5 kb,39.5 kb,7.4 kb and 4.2 kb plasmids accounted for 11.22% ,41.82% ,59.16% and 67.32% ,respectively.There were 12 types of plasmid profiles(PP), the predominant types were 7.4kb+ 4.2kb and 39.5kb+ 7.4kb+ 4.2kb, accounting for 21.42% and 17.34% ,respectively. Conclusion The gonococcal strains resistant to ciprofloxacin are very high in Zhanjiang. The phenotype and plasmid profiles of the strains have characteristic distribution.
6.The protective effects of somatostatin on acute gastric mucosal lesion in rats by pure alcohol
Ping LI ; Guoming XU ; Chenghai WANG
Academic Journal of Second Military Medical University 1982;0(01):-
To observe the protective effect of somatostatin (SS) on gastric mucosa of rats.Methods: Before gastric mucosal lesion were made in rats by pure alcohol, SS was intravenously droppedand anti-SS serum was injected into the vein, and the change of ulcer index and the degree of gastric mu-cosal lesion were observed. Results: Ulcer index of the SS group was much lower than that of the controlgroup (P
7.REGULATING ROLE OF ENDOGENOUS NITRIC OXIDE IN GASTRIC MUCOSAL BLOOD FLOW AND TOLERANT CYTOPROTECTION UNDER STRESS
Zhongmin CUI ; Zhaoshen LI ; Guoming XU
Medical Journal of Chinese People's Liberation Army 1981;0(06):-
To determine the role of GMBF in gastric mucosal tolerant cytoprotection under stress and its possible regulator, SD rats were exposed to repeated WRS, during which L NAME , a non selective NOS inhibitor, or L Arg, a substrate for NO synthesis, was administered to inhibit or promote the synthesis of NO, GMBF was measured using LDF 3 Flowmeter, NO level in gastric mucosa was monitored by Griess reaction, and gastric mucosal lesions were evaluated by UI. The results showed that gastric tolerant cytoprotection was accompanied by increased GMBF and NO level in gastric mucosa. Inhibition of endogenous NO synthesis by L NAME worsened mucosal lesions induced by WRS. After repeated WRS, adaptive increase of GMBF was abolished and NO content in gastric mucosa significantly reduced. In contrast, enhancement of endogenous NO synthesis by L Arg attenuated mucosal erosions produced by WRS and GMBF, NO content in mucosa increased. Good relationships between the changes in GMBF, UI, NO content in mucosa were found. It suggested that GMBF might play an important role in gastric mucosal tolerant cytoprotection. Endogenous NO might be one of its regulators. Inhibition of its synthesis delayed the induction of tolerant cytoprotection, while enhancementpromoted it.
8.MUTATION OF p53 IN CARCINOGENESIS OF ESOPHAGEAL TUMOR CONSEQUENT TO REFLUX OF DIFFERENT GASTRO INTESTINAL SECRETIONS
Wen WANG ; Zhaoshen LI ; Guoming XU
Medical Journal of Chinese People's Liberation Army 2001;0(10):-
The aim of the study is to evaluate the presence of mutations of p53 gene in RE and esophageal tumor induced by reflux of different gastro intestinal secretions. Gastroesophageal reflux(G), duodenoesophageal reflux(D) and duodeno gastroesophageal reflux(DG) model and control group(C) were reproduced by operations. All rats were given carcinogen (methyl n amyl nitrosamine) and their esophagi were harvested at 20, 26, 40 weeks after the operation. DNA was extracted and PCR amplification for 5, 6, 7, 8 exon of p53 followed by SSCP and AgNO 3 staining were used to detect mutation of p53 gene. It was showed that p53 gene mutations occurred in D and DG group at 20 weeks, and then more mutations were found at 26 and 40 weeks. The total rate of p53 mutation was 15 4% in groupc, 11 7% in G, 31 6% in D,and 33 3% in DG.The changes in the last two groups were significantly more compared with the former two groups.It is suggested that reflux of duodenal contents might promote mutations in p53 gene in esophageal mucosa, and reflux of gastric juice alone has lesser impact on p53 mutation.
9.CONSTRUCTION AND IDENTIFICATION OF THE VECTOR FOR CHINESE Hp MUTANT STRAINS DELETING PATHOGENICITY ISLAND
Jiong LIU ; Guoming XU ; Zhaoshe LI
Medical Journal of Chinese People's Liberation Army 2001;0(11):-
A vector for a mutant Chinese Helicobacter pylori (Hp) strains knock out pathogenicity island (PAI) was constructed, which was the basis to establish a Chinese Hp mutant deleting pathogenicity island. Genetic engineering techniques such as polymerase chain reaction, plasmid extraction, agarose gel electrophoresis, restriction analysis, ligation, preparation of competence cell and transformation were used to make two cloning fragments containing two end regions of PAI and a selectable chloramphenicol resistance marker between them, and then engineering the recombined fragments into pBluescript plasmid. The result showed that restriction analysis demonstrated that the engineering mutant vector had been recombined. It suggested that we constructed an engineering mutant vector which targeted to delete the PAI in Chinese Hp strains. It will be useful for addressing the role of PAI in the pathogenesis of Hp infection.
10.TREATMENT OF LARGE PANCREATIC PSEUDOCYST BY ENDOSCOPIC ULTRASONOGRAPHY-GUIDED CYSTOGASTROSTOMY: EXPERIENCE IN 1 CASE AND A REVIEW OF LITERATURE
Zhendong JIN ; Guoming XU ; Zhaoshe LI
Medical Journal of Chinese People's Liberation Army 2001;0(09):-
The purpose of this study was to investigate clinical feasibility of treatment of pancreatic pseudocyst by endoscopy. We reported here one case of large pancreatic psuedocyst that was treated by endoscopic ultrasonography guided cystogastrostomy.The patient's symptoms resolved on the same day of operation. And one week later, the pancreatic pseudocyst decreased by more than 50% in size. Pancreatic psuedocyst resolved and didn't relapse after 6 months. So endoscopic ultrasonography guided cystogastrostomy is a safe, reliable, effective therapy for pancreatic pseudocyst with few complications.