Objective To investigate the status of infection of human herpes virus type-8 (HHV-8) in the mother and their infants of Wulumuqi and Aletai region. Methods BCBL-1 cell line was used as antigen and 406 matched sera samples were collected from mothers and their infants (cord blood) of different nationalities (Uighur, Kazak and Han nationality) from Wulumuqi and Aletai regions of Xinjiang A.R, tested for HHV-8 IgG and IgM antibody by immunofluorescence assay. Results HHV-8 IgG antibody positive rate of mothers and their infants of Uighur nationality was 22.9%(24/105), Han was 5.4%(8/149) ? 2=17.1,P

Objective To investigate urine levels of neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule 1(KIM-1), and its relevant influencing factors in young patients with hyperuricemia.Methods Sixty young subjects with hyperuricemia were randomly selected from physical check-up examination population (the hyperuricemic group), and 60 young subjects with normouricemia were randomly selected at the same time from physical check populationexamination (the control group).Urine NGAL and KIM-1, and other biochemical indicators were evaluated.Clinical data, iab tests, testing items and biochemical indicators between the two groups were analyzed by using t test or analysis of variance test.Regression analysis between the above indicators and urinary NGAL/creatinine (cr) and urine KIM-1/cr ratios were carried by using single regression analysis.Analyzed relevant Relevant influencing factors of urinary NGAL/cr and urine KIM-1/cr ratios were analyzed by using multiple linear regression.Results Urine NGAL/cr and urine KIM-1/cr ratios in the hyperuricemic group were higher than the control group (t=22.152 5, t=24.725 5, P＜0.01).Single regression analysis showed that serum uric acid level and urine NGAL/cr ratio, KIM-1/cr ratio was positively correlations correlated (r=0.63, P＜0.01;r=0.41, P＜0.01).Multiple linear regression analysises showed that serum uric acid,systolic blood pressure, HbA1c, serum creatinin and cholesterol level were the independent factors of urine NGAI/cr ratio.Age, blood uric acid level, systolic blood pressure, HbA1c and serum creatinin levels were independent factors of KIM-1/cr ratio.Conclusion There are high levels in of urine NGAL/cr and KIM-1/cr ratios in population with hyperuricemia.Hyperuricemia, high systolic pressure, diabetes mellitus, declined kidney function and hypercholesterolemia are independent factors of urine high NGAL/creatinin ratio.Oldage,hyperuricemia, high systolic pressure, diabetes mellitus and declined kidney function are independent factors of high urine KIM-1/cr ratio.

Objective To evaluate the long term efficacy of radical cystectomy and ileal conduit in the treatment of bladder cancer. Methods 196 cases of bladder tumor treated from Jan. 1985 to Jan. 2000 with radical cystectomy and ileal conduit for urinary diversion were studied retrospectively. Results Bladder neck or trigone was involved by the neogrowth in 126(64.3%).The growth has been invasive in 145(74.0%). 93.4%(183/196) of the tumor were transitional cell carcinoma. Early operative complication occurred in 19(9.7%) and late complication in 10(7.4%).On following up for 1 to 15 years with a mean of 6.6 years,the 5 year survival rate has been 66.2%. Conclusions Radical cystectomy is the optimal in treating invasive bladder tumor and ileal conduit is a good means of urinary diversion which is simple with minimal complications.

Objective To evaluate transureteroscopic pneumatic lithotripsy in the treatment of ureteral calculi. Methods 116 cases of ureteral calculi treated with ureteroscopic pneumatic lithotripsy from Dec.1999 to Feb.2002 were studied retrospectively.There were 49 male patietns and 67 female with an average age of 43,3 being upper ureteral stone,24 middle and 68 lower ureteral stone.21 ureteral stone-street. Results The stone fragmentation rate was 90%(104/116),85%(99/116) of which being fragmented on the first treatment session.The main complications were failure to insert the ureteroscope in 3 cases and ureteral perforation in 4.94 cases were follow-up for one to eighteen months and 91 cases were stone free within one month.No ureteral stricture has been observed on intravenous urogram. Conclusions Treatment of ureteral calculi by transureteroscopic pneumatic lithotripsy has been safe, effective and less invasive.

0.01). There was no recurrence in both groups. Mean hospitalization stay was 8.1 days in the laparoscopy group, whereas it was 11.8 days in the open surgical group. Post- operative fever lasted a mean of 4.1 and 5.4 days in the two groups respectively ( P

objective To examine nuclear transIocation of peroxisome proliferator-activated receptor γ(PPARγ)in rats following focal cerebral ischemia/reperfusion(I/R),and to explore the significance of altered PPARγ,nuclear translocation in ischemic brain injury.Methods Healthy adult male SD rats underwent 60-min cerebral artery occlusion followed by reperfusion of 4,8,or 24 h,respectively.The cytoplasmic-to-nuclear shuttling of PPARγ was characterized by Western blot,immunohistochemical and immunofluoreseence staining.The effects of PPARγ agonist rosiglitazone (Ros) and antagonist GW9662 on I/R-induced PPARγ nuclear translocation were also examined in the present study. Furthermore,TTC staining war adopted to determine the change in cerebral infarction volume. Results (1)Western blot analysis revealed an increase of PPARγ in the nucleus and a simultaneous reduction in the cytosol following ischemia and reperfusion for 4 h(tcytosol=9.03,tmuclear=27.19,P=0.00).Prolonged the reperfusion further enhanced this I/R induced PPARγ translocation in a time-dependent manner.Using immunohistochemistry and immunofluorescence,nuclear PPAR γ positive staining increased from 48.3%in the sham control to 80.3% following ischemia and reperfusion for 24 h.(2)Western blot analysis revealed that PPARγ agonist Ros further increased I/R-induced nuclear enrichment of PPARγ,whereas PPARγ antagonist GW9662inhibited I/R-stimulated change in PPARγ.(3)When compared to the L/R group using TTC staining,Ros treatment significantly decreased the infarction volume by 48.40%(15.46±4.94 versus 29.96±3.39,t=5.93.P=0.00),whereas GW9662 increased by 58.95%(47.62±4.93 versus 29.96±3.39,t=7.23,P=0.00).Conclusions Cerebral I/R injury induces PPARγ translocation from the cytosol to the nucleus.This change may represent an intrinsic neuroprotective response against brain I/R injury.

Teaching competency is one of the basic skills for teachers to accomplish daily teaching routine.This article focused on issues like imbalanced development in teaching competency,young teachers' lack of solid basic teaching ability,teachers' weak research ability and their lack of research ability to support teaching process in local medical colleges.In theory it systematically explained the teachers' teaching competency structure.Through the implementation of three linkage training of university-faculty-teaching and research department,normalized and institutionalized training for teachers from teaching-hospital,cooperation between clinical and basic medicine teachers,teachers' basic teaching ability was improved.And by transforming teaching evaluation philosophy,setting teaching quality standards at different levels,strengthening teachers' teaching introspection,teachers' ability in teaching evaluation was increased.Besides,by delivering encouragement policy and project driven teachers were guided to upgrade research ability in order to reflect on teaching.

Objective:To investigate the immediate bond strength and surface structure of resin and the tooth enamel which treated by cold plasma.Methods:In the study,40 bovine incisors were divided into two equal parts.In this sense,all enamel adhesive samples were prepared and then randomly divided into 4 groups(n =20).group 1:acid +single bond 2 +resin composite(control group);group 2:beyond bleaching+acid+single bond 2+resin composite;group 3:treated by cold plasma for 5 minutes+acid+single bond 2+resin composite;group 4:treated by cold plasma for 5 minutes+single bond 2+resin composite.Single bond 2 bonding system and Filtek Z250 resin were used in this experiment.The shear bond strength was tested by universal testing machine.The surface of the enamel in different processes was observed by scanning electron microscope (SEM).Statistical analyses by the single factor analysis of variance and multiple pairwise comparisons were performed with SPSS 1 7 .0 .Results:The shear bond strength of group 4 (8.60 MPa)was significantly lower than that of the other three groups (P<0.05). The shear bond strength of group 2 (1 7.89 MPa)was higher than that of group 4,but lower than group 1 and group 3 (P<0.05).There was no significant difference between group 1 (34.82 MPa)and group 3 (34.69 MPa).Scanning electron microscope indicated that the enamel treated by cold plasma had slight molten form,which was different from etched enamel surface.The fractured surface of group 3 was mix fracture,which was similar to the control group (group 1 ).Conclusion:Compared with the conven-tional clinic bleaching,immediate bond strength of resin-enamel that treated by cold plasma has not been affected.

Objective To study the diagnosis and treatment of cystic renal cell carcinoma. Methods 12 cases of cystic renal cell carcinoma have been identified. Imaging studies showed no specific evidence of malignance and the preoperative diagnosis was a complex cystic mass in all but 2.All the 12 cases had been surgically explored,intraoperative pathological examination being undertaken in 9,8 of them demonstrating a malignant cystic renal clear cell carcinoma.Radical nephrectomy was carried out in 7,simple nephrectomy in 3,partial nephrectomy in 1 and one cyst unroofed. Results All the 12 cases were confirmed as cystic renal clear cell carcinoma on pathology.All the patients have been followed up for a mean of 39.5 months and the overall results were excellent with no evidence of cancer recurrence or metastasis. Conclusions Intraoperative pathological examination should be performed in suspected cases of cystic renal cell carcinoma.The prognosis is fair for cystic renal clear cell carcinoma.Nephrectomy or partial nephrectomy is indicated.

Objective To explore the clinical features, diagnosis and treatment of Wunderlich syndrome (WS).Methods Fifteen patients with WS were included from September 2008 to February 2014, and their clinical features, diagnosis and treatment were retrospectively reviewed.The most common clinical manifestations were flank or abdominal pain (15/15), hypovolemic shock (5/15), gross hematuria (4/15) and percussion pain on kidney region ( 15/15 ) .Laboratory tests showed anemia ( 9/15 ) and coagulation abnormalities (5/15).Five of 15 cases were critical patients with moderate to severe shock ( systolic blood pressure≤90 mmHg, 1 mmHg =0.133 kPa ) accompanied with severe anemia ( Hb <60 g/L ) and coagulation abnormalities.Results In the acute stage, the diagnostic ratios of ultrasonography and contrast enhanced CT for WS were 11/15 and 15/15, and cause determination ratios were 4/15 and 10/15, respectively.The latter was significantly higher than the former ( P <0.05 ) .Contrast enhanced MRI was performed in 5 cases, and the results were identical to those of CT.According to the causes made by emergent imaging, critical patients underwent emergent operations or renal arteriography plus selective arterial embolization, and the other patients underwent conservative management, emergency operations, or renal arteriography plus selective arterial embolization, respectively.The causes of WS included angiomyolipoma (8 cases), renal cell carcinoma (3 cases), metastatic tumor of lung cancer (1 case) and renal cyst (3 cases) in this study.The mean follow-up period was 34 months.One critical patient died, and all the other patients were cured or relieved.Conclusions WS has no specific clinical features.Contrast enhanced CT or MRI is the main approach for diagnosis and cause determination, which is superior to ultrasonography.Treatments for WS vary according to severity classification and imaging diagnosis.