Objective To investigate the effects of ?-aescin on nuclear factor-?B (NF-?B) activities and tumor necrosis factor-? (TNF-?) protein expression in the rat brain tissue following acute traumatic brain injury (TBI). Methods A total of 62 SD rats were subjected to a lateral cortical impact injury caused by a free-falling object and divided randomly into four groups, ie, sham operation group (Group A), injury group (Group B), ?-aescin treatment group (Group C) and pyrrolidine dithocarbamate (PDTC) treatment group (Group D). Group C was administered with ?-aescin and Group D treated with PDTC immediately after injury. A series of brain samples were obtained directly 6, 24 hours and three days respectively after operation in four groups. The NF-?B activation of rat brain was determined by electrophoretic mobility shift assay (EMSA) and the levels of TNF-? protein in rat brain measured by radio-immunoassay (RIA). In the meantime, the water content of rat brain was measured and pathomorphological observation carried out. Results Compared with Group A, NF-?B activities, the levels of TNF-? protein and the water content of the rat brain were significantly increased (P

Objective To analyze the safety and efficacy of early diet after laparoscopic resection of colorectal cancer.Methods A prospective study of 128 patients suffered laparoscopic surgery of colorectal cancer was done in our hospital.According to whether the adoption of early diet program,patients were divided into early oral group (EOF group,n=54)and traditional oral diet group (TOF group,n=74).Age,gender,preoperative nutritional status and other basic factors and postoperative ambulation time, postoperative hospital stay time,the number of cases the use of analgesics,complications of two groups were analyzed statistically. Results The first time of postoperative ambulation time and postoperative hospital stay time of EOF group were significantly re-duced compared TOF group [(1.2±0.8)d vs .(2.5±1.3)d]and [(3.2±1.4)d vs .(5.3±1.6)d](P <0.01).Conclusion Early diet after laparoscopic resection of colorectal cancer is good for patients fast recovery,and postoperative complications of two groups are similar.

Objective To investigate the effects of antioxidant SS-31 on sepsis-induced acute lung injury (ALI)in a mouse model of sepsis.Methods Sepsis was induced in male mice by cecal liga-tion and puncture (CLP).Forty-eight adult male mice (C57BL/6,weight 25-32 g)were equally as-signed to the sham+vehicle group (group A),sham+SS-31 group (group B),CLP+vehicle group (group C),or the CLP+SS-31 group (group D).At 0 or 5 h after CLP or sham operation,mice re-ceived an intraperitoneal injection of SS-31 (5 mg/kg of body weight)or the same volume of normal saline.Pulmonary tumor necrosis factor alpha (TNF-α),interleukin (IL)-6,IL-10,malondialdehyde (MDA),superoxide dismutase activities (SOD),myeloperoxidase activities (MPO ),wet-to-dry weight ratio (W/D),reactive oxygen species (ROS),ATP,NF-κB p65,inducible nitric oxide syn-thase (iNOS),and histological scores were assessed 24 h after operation.Results Pneumonia,edema were significantly heavier in group C than in group A (P <0.05).Lung congestion,inflammatory cell infiltration,alveolar wall edema was significantly less in group D than in group C (P <0.05).Pulmo-nary histological scores,IL-6,MDA,MPO,W/D,ROS,NF-κB p65 and iNOS significantly in-creased,while ATP levels decreased in group C when compared with group A (P <0.05).However, SS-31 treatment significantly reversed these parameters when compared with the group C (P <0.05). No difference was observed between the group A and group B.There was no difference of TNF-α,IL-10,and SOD among the four groups.Conclusion SS-31 improves sepsis-induced ALI in a mouse model probably by down-regulating the oxidative stress and inflammation in of sepsis-induced ALI.

Objective:To explore effects of nurses' cognition of patient safety culture and psychological capital on career growth, so as to provide a basis for promoting nurses' career growth.Methods:This research was a cross-sectional survey. From July to August 2019, a total of 480 nurses from 3 ClassⅢ Grade A hospitals in Tianjin were selected as the research objects by means of convenient sampling. General information questionnaire, Hospital Survey on Patient Safety Culture (HSOPSC) , nurses' Psychological Capital Questionnaire (PCQ) and Career Growth Scale (CGS) were used to investigate the patients. In this study, 480 questionnaires were issued and 465 valid questionnaires were collected, with an effective recovery rate of 97%. Stratified regression analysis was used to analyze the mediating effect of psychological capital, and AMOS was further used to construct the structural equation model for verification.Results:The total mean score of HSOPSC of 465 nurses was (3.91±0.30) , the total mean score of PCQ was (4.66±0.35) and the total mean score of CGS was (3.62±0.41) . Nurses' cognition of patient safety culture was positively correlated with career growth ( r=0.525, P<0.01) . Nurses' cognition of patient safety culture was positively correlated with psychological capital ( r=0.470, P<0.01) . There was a positive correlation between psychological capital and career growth ( r=0.557, P<0.01) . The direct path coefficient of patient safety culture cognition on career growth was 0.33. Psychological capital played a part of the mediating role in the relationship between patient safety cultural cognition and career growth. The mediating effect was 0.30, and the mediating effect accounted for 47.6% of the total effect. Conclusions:The career growth of nurses is at a medium level, and psychological capital plays an intermediary role between the patient's cultural awareness of safety and career growth. Nursing managers should take effective measures to improve nurses' cognition of patient safety culture, at the same time improve nurses' psychological capital level, so as to promote nurses' career growth.

OBJECTIVETo study the biocompatibility of porous calcium phosphate ceramics nanocomposite.

METHODSThe biocompatibility was evaluated via experiments including the hemolysis test, hemopexis test, acute systemic toxicity test, pyrogen test, and intramuscular implant test, in which biphasic calcium phosphate nanocomposite (NanoBCP) presented as leaching solution, suspension or blocks of 5 mmx5 mmxl mm. Animals including New Zealand Rabbits, Kunming mice, SD rats were selected as the host.

RESULTSThe hemolysis of NanoBCP was 1.1% (<5%). Four coagulation index levels were within the normal range. In pyrogen test, the temperature of each experimental rat increased by 0.35, 0.40, 0.28 degrees C (<0.60 degrees C, in accordance with the pyrogen-free criterion for biomedical materials). No consequent death, dyspnoea, organ dysfunction, severe peritoneal irritation or ptosis was observed in acute systemic toxic test. Newly-formed fibrous tissue could be found after the implantation.

CONCLUSIONThe material possesses outstanding biocompatibility and degradability with no toxicity or irritation, contains no pyrogen, as well as better degradation properties than biphasic calcium phosphate.

Animals ; Biocompatible Materials ; Calcium Phosphates ; Ceramics ; Hydroxyapatites ; Mice ; Nanocomposites ; Prostheses and Implants ; Rabbits ; Rats ; Rats, Sprague-Dawley

Objective To explore the expression of RIP2 in colorectal cancer(CRC)tissues,and the inva-sion and migration was observed after RIP2 was increased in CRC cell line.Methods 48 cases of CRC paraf-fin-embedded specimens and 56 cases of tumor-adjacent tissues were collected,and expressions of RIP2 was tested by immunohistochemistry(IHC).pEGFP-RIP2 plasmid and JetPRIME were employed to increase the RIP2 in SW480 cells,and variety of invasion and migration was tested in SW480 cells.Results It was showed that expressions of RIP2 was lower in CRC tumors than in tumor-adjacent tissues(P<0.05).After RIP2 in-creased,migration ability of SW480 cells weakened.Conclusion The expression of RIP2 was decreased in CRC tissues,and it was closely related to tumor cell's invasion and metastasis.

Objective To investigate the effects of Nod-like receptor protein 3 inhibitor MCC950 on cognitive function in a mouse model of sepsis-associated encephalopathy (SAE).Methods Ninety adult male C57BL/6 mice were randomly (random number) divided into three groups:the sham + saline group (n=20,sham group),CLP + saline group (n=35,CLP group),and CLP + MCC950 group (n=35,MCC950 group).SAE mouse model was established by cecal ligation and puncture (CLP) surgery.Saline (10 mL/kg) or MCC950 (10 mg/kg) was intraperitoneally injected 30 min before surgery and on day 1,2,4 and 6 after surgery according the grouping.Seven days after surgery,six mice were taken from each group.Western blot was used to detect the hippocampal content of NLRP3,apoptosis-associated specklike protein (ASC),interleukin-1β (IL-1β) and IL-18.The number of NLRP3-positive cells in CA 1 region were detected by immunohistochemical analysis.The remaining mice in each group were used for open field and fear conditioning tests 14 days after surgery.One-way analysis of variance was used for inter-group comparison,and SNK-q test was used for pairwise comparison.A P value ＜0.05 was considered statistically significant.Results Compared the MCC950 group with the CLP group,the freezing time of context test was significantly increased [(137±21) s vs (84±15) s,P=0.013],the hippocampal content of NLRP3,IL-1β and IL-18 were significantly reduced (P＜0.01),and the number of NLRP3-immunoreactive cells per mm2 in CA region were significantly decreased (23±5 vs 74±13,P＜0.01).There was no significant changes in protein level of ASC and results of open field tests (P＞0.05).Conclusions MCC950 administration can improve cognitive function in a mouse model of SAE,which is probably due to the inhibition of NLRP3 inflammasome and downstream inflammatory cytokines IL-1β and IL-18.

Objective To explore the characteristics of epidermal growth factor receptor (EGFR)mutation in patients with lung adenocarcinoma in Xinjiang automonous region.Methods 181 patients with lung adenocarcinoma were collected (Uighur:71 cases;Han:105 cases).The clinical characteristics of each patient was recorded,and the paraffin embedded tumor tissue was col-lected.Exons 18~21 of EGFR genes were detected by ARMs.The mutation rates of EGFR genes were compared in Uyghur and Han nationalities,and the relationship between EGFR gene muta-tions and clinical ccharacteristics were analyzed.Results EGFR mutation rate was 32.6% in all patients ,in which the mutations rate in Han patients (4 5 .7 % )was higher than Uyghur patients (15.8%).The main mutation types were the deletion of exon 19 and L858R of exon 21.The dele-tion of exon 19 was more common in Uyghur patients.In Han patients,EGFR mutation was relat-ed to the gender,smoking history and the differentiated degree of tumors,whereas Uyghur patients was only associated with smoking history.Conclusion For Uyghur patients,EGFR mutation rate is lower than Han patients in the same area,and exon 19 sensitive mutation is more frequent.

Objective To explore the characteristics of epidermal growth factor receptor (EGFR)mutation in patients with lung adenocarcinoma in Xinjiang automonous region.Methods 181 patients with lung adenocarcinoma were collected (Uighur:71 cases;Han:105 cases).The clinical characteristics of each patient was recorded,and the paraffin embedded tumor tissue was col-lected.Exons 18~21 of EGFR genes were detected by ARMs.The mutation rates of EGFR genes were compared in Uyghur and Han nationalities,and the relationship between EGFR gene muta-tions and clinical ccharacteristics were analyzed.Results EGFR mutation rate was 32.6% in all patients ,in which the mutations rate in Han patients (4 5 .7 % )was higher than Uyghur patients (15.8%).The main mutation types were the deletion of exon 19 and L858R of exon 21.The dele-tion of exon 19 was more common in Uyghur patients.In Han patients,EGFR mutation was relat-ed to the gender,smoking history and the differentiated degree of tumors,whereas Uyghur patients was only associated with smoking history.Conclusion For Uyghur patients,EGFR mutation rate is lower than Han patients in the same area,and exon 19 sensitive mutation is more frequent.