The high mobility group A2 (HMGA2), one ofnon-histone chromatin proteins, may alter chromatin structure and thereby regulate the transcription of several genes by either enhancing or suppressing transcription factors, and leading to malignant neoplasm formation. This paper focuses on the role of the HMGA proteins in human neoplastic diseases, and discusses the mechanisms by which they contribute to carcinogenesis, and diagnosis strategies based on targeting HMGA proteins.

Bladder cancer is a common type of tumor in the urinary system. Its incidence has been increasing and the disease has a high rate of recurrence. Cystoscopy and cytology are the main methods for the diagnosis and the early detection of recurrence for bladder transitional cell carcinoma. Since cystoscopy is expensive and invasive, and although cytology is non-invasive but it is not very sensitive, many tumor makers have recently been studied in the diagnosis of the disease. So how to choose tumor makers with high sensitivity and specificity for the diagnosis of the disease so that the patients with bladder cancer could be treated at the earliest stages is important, the biomarkers may play a very important role in clinical application. In this review we discussed the development of biomarkers for bladder cancer.

[Objective] To study the cure effect of Ganle Infusion on hepatitis-B of depressed liver and deficient spleen type.[Method] Randomly divide 60 cases into treatment group(Ganle Infusion) and control group(Diammonium Glycyrrhizinate capsule),observe concerned indexes change and clinical effects.[Result] For treatment group,the cure effect index was 93.3%;66.7% for control one;on liver improvement and serum hepatitis-B virus index negative-transfer rates,the treatment group was better than control one,with marked difference.[Conclusion] Ganle Infusion has marked cure effect on chronic hepatitis-B of the said type.

Objective To observe the effects of mesenchymal stem cells (MSCs)injection from tail-vein on the expression of glial cell line derived neurotrophic factor (GDNF) after the spinal cord injury (SCI) of rats.MethodMSCs were cultured from the thighbone of adult wistar rats.The T10 level spinal cord injury was execute by the modify-Allen's device. The MSCs labeled by bromodeoxyuridine were transplanted into the vein of tail by injection immediately after spinal cord injury. Adult Wistar rats were randomly divided into three groups: SCI cured with MSCs transplantation (group A), SCI received normal saline(group B),and control group (group C). Then MSCs were detected and the expressions of GDNF of the lesion and neighbor areas were examined by Reversetranscription polymerase chain reaction (RT-PCR) and immunohistochemistry.ResultImmunohistochemistry: MSCs labeled by bromodeoxyuridine could be detected in the injuried spinal cord after transplantation. It was located at the nucleolus of MSCs. The cells survived and migrated rostrallyand caudally from the injection sites. With the time passed, the number of MSCs labeled with bromodeoxyuridine was decreased. RT-PCR: The expression of Group A was increased on the followed day, and the expression of Group B was increased on the 1st day but decrease from the 5th day then on.compared with group B, transplantation of MSCs significantly enhanced the expression of GDNF mRNA than group A on the 1st, 3rd, 5th day after cell transplantation(P

Objective To observe the expression and location of galactose β-1,4-glycosidic bonds (Gal β-1,4-GlcNAc) in keloid tissue,and to investigate the role of glycoprotein galactosylation in the formation of keloid.Methods This study included 10 keloid tissue specimens,7 hyperplastic scar tissue specimens,and 6 normal skin specimens.Lectin blot analysis was performed to measure the glycosylation level of glycoproteins,saturated picric acid-Sirius red staining followed by polarization microscopy to observe the type,expression and distribution of collagens in these specimens.The type Ⅰ/type Ⅲ collagen ratio was calculated.Immunofluorescence-based histochemistry was carried out by using Ricinus communis agglutinin I to analyze the expression and location of Gal β-1,4-GlcNAc in these skin samples,and double immunofluorescent staining to observe the colocalization of Gal β-1,4-GlcNAc and type Ⅰ procollagen α1.Results Compared with the normal skin tissue,the keloid tissue showed a significant increase in the expression of Gal β-1,4-GlcNAc in glycoproteins with a relative molecular mass of 30 000 to 40 000.Polarization microscopy revealed that there was a considerable expression of type Ⅰ collagen fibers,which amounted to (71.53 ± 4.03)％ in all the collagen fibers.The type Ⅰ/type Ⅲ collagen ratio was significantly higher in keloid tissue specimens than in normal tissue specimens (2.56 ± 0.53 vs.0.91 ± 0.11,P ＜0.05).Fluorescence microscopy showed that Gal β-1,4-GlcNAc was uniformly distributed in the membrane and cytoplasm of fibroblasts in keloid tissue,and the expression intensity of Gal β-1,4-GlcNAc in keloid tissue was notably stronger than that in normal skin tissue.There was a colocalization between Gal β-1,4-GlcNAc and type Ⅰ procollagen α1 in keloid tissue.Conclusions The expression of Gal β-1,4-GlcNAc,which is mainly observed in fibroblasts,is upregulated in keloid tissue,suggesting that Gal β-1,4-GlcNAc may be involved in the modulation of factors responsible for excessive fibre formation during the repair process of keloid.

Objective o observe the therapeutic effects of suspension-assisted functional rehabilitation training of motor function for patients with cerebral infarction. Methods Ninety-six patients with cerebral infarction were divided randomly into a treatment group and a control group. All patients were given routine medical treatment and routine limb movement therapy. Those in the treatment group also used an electrically-driven suspension device to aid in their functional rehabilitation training. The patients were assessed with a simplified Fugl-Meyer Motor Functional Assessment (S-FMMFA), and using the Postural Assessment Scale for stroke patients ( PASS), the Barthel Index (BI) and the 6-minute walking distance test before and after treatment and at a 6-month foliowup. Results The average S-FMMFA, PASS, BI and the 6-minute walk results in the treatment group were all significantly better than those in the control group. Conclusions Suspension-assisted training can significantly help improve limb movement function and ADL performance in cerebral infarction patients.

Objective:To investigate the pain in patients during the early stages of recovery from maxillofacial surgery under general anaesthesia.Methods: One hundred patients as participants to respone the questionaire of postoperative pain 24 hours after operation. The data of pain intensity,analgesia requirement and the reason for operation of the patients were collected and analyzed. Results: Among the 100 cases, 92 with moderate or slight postoperative pain, 8 with severe pain. It is significant more severe pain (14.3%)in the group of bone fracture than that (less than 8.7%) in other groups. Conclusion: Most patients 24 h after maxillofacial surgery are with moderate and slight pain. The pain intensity relates with type of maxillofacial surgery.

In dynamic state,the airflow and particle distribution of class Ⅲ biosafety laboratory is simulated by means of the CFD.The identical results of the simulation and the practical survey show good reliability of this experiment.The result indicates that the good unidirectional air distribution is formed in main lab and air distribution is the main factors to determine the character of particle distribution.

The paper elaborates on the design thinking of the outpatient intelligent medicine delivery system under the two parallel Hospital Information Systems (HIS) of the Central Hospital of Wuhan and implements the distribution of medicine delivery windows based on the multi-channel payment methods of the old HIS outpatient system and the preplanned and real-time intelligent medicine delivery based on the new HIS pharmacy system.Using the integrated platform of Ensemble and services of Web Service,it realizes the data interaction among multiple systems.The system operates steadily and the new HIS is well connected with the old.Thus,the efficiency can be enhanced and the quality can be ensured.

Objective To observe the results and side effects of radiotherapy(RT) combined with hyperthermia(HT) for non-small cell lung cancer(NSCLC)with lesion(T) ≥5?cm in diameter.Methods Patients conforming to T≥5?cm or T volume ≥125?cm~3 in the lung and other required eligibilities were given RT and HT.The temperature of HT in the center of Ts was automatically programmed between 43.0℃ and 43.5℃ by a computer.RT dose of D_T60-66?Gy was delivered in 30-33 fractions with in 1 hour after HT.Results From October 2001 to April 2004,30 patients were registered for this study.Among all patients,23 males and 7 female with median age of 61 years(32-81);22 squamous cancer and 8 adenocarcinoma type;6 stage Ⅰb,1 Ⅱb,10 Ⅲa,8 Ⅲb and 5 Ⅳ lesions.The mean volumes of T was 275.45?cm~3(125-900?cm~3).The mean number of HT given was 7.2 times(2-14).The mean irradiation dose was 59.10?Gy(23.96-9.67?Gy).Five out of 30 patients were dropped from this trial due to reasons un-related to acute side effects of RT or HT.None complained of breathlessness or aggravation of respiratory symptom during heating.No change had been observed in blood pressure,pulse or frequency of breath before or after HT,nor was there any hardening of subcutaneous fatty tissue as well as lipid nodules or severer than grade Ⅲ acute irradiation side effects.Complete response was observed in 23%,partial response in 50%,no change or progression in 27%.Loco-regional control rate was 83% with a median of median locoregional duration of 19.2 months.Eleven patients had died,including 2 of hemoptysis.The median survival duration was 19.7 months and 1-year survival rate was 75%.Conclusions Radiotherapy combined with hyperthermia modal can be used for NSCLC with primary lesion≥5?cm as it carries better efficiency and loco-regional control without server side effects.