Objective:To observe the effects of Salvia Miltiorrhiza Bunge on cell proliferation and differentiation of osteoblasts in rats cultured in vitro.Methods:Osteoblasts from cranium of newborn SD rats were cultured by collagenase method.MTT was used to determine the proliferation of cultured osteoblasts affected by different concentrations of Salvia Miltiorrhiza Bunge at different time points(24,48,72 hrs after medication).Differentiating effect of different concentrations of Salvia Miltiorrhiza Bunge in the cell was evaluated through the examination of alkali phosphate(ALP) activity.Results:0.05%,0.10%,0.15% of Salvia Miltiorrhiza Bunge could enhance the cell proliferation rate(P

To investigate the influence of abnormal blood coagulating state on development of diabetic nephropathy. Methods Using radioimmunoassay and bivanlent antibody sandwich technic, the levels of plasma protein C (PC), platelet alpha-granule membrane glycoprotein (GMP-140), and factor Ⅷ: C and vWF were detected in 65 NIDDM patients and 15 normal controls. Results (1)PC level in microalbuminuric patients(DMⅡ) was significantly lower than that in normal controls and in normoalbuminuric patients (DMⅠ ), the lowest was in clinical proteinuric patients (DMⅢ); PC levels correlated negatively with urinary albumin exeretion rates(UAER). (2)The GMP-140 level was higher signifi-antly in DMn than in normal controls and in DMⅢ ; and the highest was in DMⅠ; the GMP-140 levels positively correlated with UAER. (3)There were negative correlations between PC and GMP-140、Ⅷ C-vWF. Conclusion A hyper-coagulable state occurs early in diabetic nephropathy as valuable indexes in reflecting the microangiopathy in diabetes.

Type 2 diabetic patients with the decline of urinary Tamm-Horsfall protein excretory rate showed the increment of fasting plasma homocysteine levels, suggesting that the damage of synthetic function of renal tubule may participate in the increment of homocysteine levels.

That there are so many difficulties in both of teaching and learning electrocar-diogram(ECG)is the characteristics in teaching and studying ECG. The following ways are the ef-fective methods of improving effects of classroom teaching of ECG:emphasizing the importance of ECG in clinical work at the beginning of the lecture on ECG in order that the students can understand the necessity of studying ECG and make up their mind to study ECG well;studying the teaching contents on ECG seriously and supplement the textbook appropriately;set up the link of discussion and create the condition that teacher and students work altogether;produce the slides with pictures(mainly)and words and give accounts of the pictures mainly;sum up the character-istics of all sorts of abnormal ECG and state the characteristics of presenting ECG simultaneously.

AIM: To explore the effects of high glucose a t fluctuant concentrations on glucose transport activity and expression of phosp hoinositide 3-kinase (p85) in primary cultured skeletal muscle cells. ME T HODS: Rat skeletal muscle cells were cultured at fluctuant glucose conce ntrations (5, 25mmol/L) for 48h. Then the glucose uptake and the expression of p hosphoinositude 3-kinase were measured. RESULTS: The skeletal mu s cle cells treated with fluctuant high concentration glucose showed the impairmen t of the basal and insulin-induced increase in glucose uptake and significant de crease in p85 protein expression as well as p85 mRNA. Particularly, there was a significant decrease in p85 protein and mRNA expression in the high fluctuate l evel of glucose concentration. CONCLUSION: The exposure to fluct uant high concentration of glucose inhibits glucose uptake and induces insulin r esistance in skeletal muscle cells.

Objective o observe the therapeutic effects of suspension-assisted functional rehabilitation training of motor function for patients with cerebral infarction. Methods Ninety-six patients with cerebral infarction were divided randomly into a treatment group and a control group. All patients were given routine medical treatment and routine limb movement therapy. Those in the treatment group also used an electrically-driven suspension device to aid in their functional rehabilitation training. The patients were assessed with a simplified Fugl-Meyer Motor Functional Assessment (S-FMMFA), and using the Postural Assessment Scale for stroke patients ( PASS), the Barthel Index (BI) and the 6-minute walking distance test before and after treatment and at a 6-month foliowup. Results The average S-FMMFA, PASS, BI and the 6-minute walk results in the treatment group were all significantly better than those in the control group. Conclusions Suspension-assisted training can significantly help improve limb movement function and ADL performance in cerebral infarction patients.

Objective: Our purpose was to understand the effect of transforming growth factor-β(TGF-β) in pathogenesis and development of diabetic nephropathy (DN). Methods: Serum TGF-β was measured with enzyme-linked immunosersorbent assay(ELISA). Results: The serum level of TGF-β in diabetic patients with no DN was not different from that in normal persons(12.7±5.0 pg/ml, 12.6±4.4 pg/ml, P >0.05). Serum TGF-β level in DN patients with urinary albumin clearance (20 to 200 μg/min，not include 200) was significantly higher than that in diabetic patients with no DN (65.3±13.0 pg/ml, 12.7±5.0 pg/ml, P<0.01). Serum level of TGF-β in DN patients with urinary albumin clearance (≥200 μg/min) was significantly higher than that in the patients with trace urinary albumin （136.4±21.4 pg/ml, 65.3±13.0 pg/ml, P<0.01）, and serum level of TGF-β was positively correlated with urinary albumin clearance. Conclusion: The serum level of TGF-β has increased in early stage of DN. With the development of DN, the serum level of TGF-β significantly increased. The alteration of serum level of TGF-β was positively correlated with urinary albumin clearance. So TGF-β play a very important role in pathogenesis and development of diabetic nephropathy. We can regard serum level of TGF-β as a diagnostic target of DN in early stage.

Transcatheter arterial chemoembolization (TACE) has already been a mature and an effective treatment for advanced hepatocellular carcinoma (HCC).Clinically,it is very important to quickly and accurately evaluate the postoperative curative effect with minimally invasive technique so as to determine the next treatment options.At present,postoperative conventional CT and MRI are the main means to assess the curative effect of TACE,but it is a pity that after the treatment the functional changes of the tumor occur earlier than the morphological changes.In recent years,functional MRI techniques,such as diffusionweighted imaging (DWI),multi-b value DWI,dynamic contrast-enhanced (DCE) imaging,etc.have been more and more used for quantitative evaluation of the diffusion of water molecules and the blood microcirculation perfusion within the tumor tissue,and some progresses have been achieved in the evaluation of curative efficacy for tumor.This paper aims to make a comprehensive review about the research progress of the above mentioned functional imaging methods as well as their current application status in evaluation of the curative effect of TACE.

Plasma VEGF level of T2DM was higher than that of normal control （NC, n=30） and progressively increases from normal albuminuria （NA, n=26） to microalbuminuria （MA,n=26） and to macroalbu-minuria （ODN, n=24） groups, The VEGF level was positively correlated with the levels of creatinine, HbA1c and UAER and, decreases after losartan treatment in groups of MA and ODN.

Objective To discuss the expression and the related factors of E-selectin in insulin-resistant(IR)rat endothelium. Methods Wistar rats were randomly divided into 3 groups: high fat feed,high fructose feed and the control feed(HF group,HG group and NC group). IR rat model was established by high fat feeding or high fructose feeding for 4 weeks and was evaluated with euglycemic clamp technique. E-selectin protein expression was detected by Western-blot analysis. Results The expression of E-selectin protein in vessel endothelium was increased in HF group and HG group compared with that in NC group (P<0.01), and higher in HF group than in HG group (P<0.05). E-selectin protein expression was correlated significantly with TG, FFA, FIns and HOMA-IR in HF group and HG group (P<0.05). Conclusions IR may cause the increased expression of E-selectin in endothelium of rats fed with high fat or fructose.