Objective:To observe the effects of Salvia Miltiorrhiza Bunge on cell proliferation and differentiation of osteoblasts in rats cultured in vitro.Methods:Osteoblasts from cranium of newborn SD rats were cultured by collagenase method.MTT was used to determine the proliferation of cultured osteoblasts affected by different concentrations of Salvia Miltiorrhiza Bunge at different time points(24,48,72 hrs after medication).Differentiating effect of different concentrations of Salvia Miltiorrhiza Bunge in the cell was evaluated through the examination of alkali phosphate(ALP) activity.Results:0.05%,0.10%,0.15% of Salvia Miltiorrhiza Bunge could enhance the cell proliferation rate(P

To investigate the influence of abnormal blood coagulating state on development of diabetic nephropathy. Methods Using radioimmunoassay and bivanlent antibody sandwich technic, the levels of plasma protein C (PC), platelet alpha-granule membrane glycoprotein (GMP-140), and factor Ⅷ: C and vWF were detected in 65 NIDDM patients and 15 normal controls. Results (1)PC level in microalbuminuric patients(DMⅡ) was significantly lower than that in normal controls and in normoalbuminuric patients (DMⅠ ), the lowest was in clinical proteinuric patients (DMⅢ); PC levels correlated negatively with urinary albumin exeretion rates(UAER). (2)The GMP-140 level was higher signifi-antly in DMn than in normal controls and in DMⅢ ; and the highest was in DMⅠ; the GMP-140 levels positively correlated with UAER. (3)There were negative correlations between PC and GMP-140、Ⅷ C-vWF. Conclusion A hyper-coagulable state occurs early in diabetic nephropathy as valuable indexes in reflecting the microangiopathy in diabetes.

That there are so many difficulties in both of teaching and learning electrocar-diogram(ECG)is the characteristics in teaching and studying ECG. The following ways are the ef-fective methods of improving effects of classroom teaching of ECG:emphasizing the importance of ECG in clinical work at the beginning of the lecture on ECG in order that the students can understand the necessity of studying ECG and make up their mind to study ECG well;studying the teaching contents on ECG seriously and supplement the textbook appropriately;set up the link of discussion and create the condition that teacher and students work altogether;produce the slides with pictures(mainly)and words and give accounts of the pictures mainly;sum up the character-istics of all sorts of abnormal ECG and state the characteristics of presenting ECG simultaneously.

Type 2 diabetic patients with the decline of urinary Tamm-Horsfall protein excretory rate showed the increment of fasting plasma homocysteine levels, suggesting that the damage of synthetic function of renal tubule may participate in the increment of homocysteine levels.

AIM: To explore the effects of high glucose a t fluctuant concentrations on glucose transport activity and expression of phosp hoinositide 3-kinase (p85) in primary cultured skeletal muscle cells. ME T HODS: Rat skeletal muscle cells were cultured at fluctuant glucose conce ntrations (5, 25mmol/L) for 48h. Then the glucose uptake and the expression of p hosphoinositude 3-kinase were measured. RESULTS: The skeletal mu s cle cells treated with fluctuant high concentration glucose showed the impairmen t of the basal and insulin-induced increase in glucose uptake and significant de crease in p85 protein expression as well as p85 mRNA. Particularly, there was a significant decrease in p85 protein and mRNA expression in the high fluctuate l evel of glucose concentration. CONCLUSION: The exposure to fluct uant high concentration of glucose inhibits glucose uptake and induces insulin r esistance in skeletal muscle cells.

0.05).(4)The para-operation pulmonary function indexes(VC,VC%,FEV1)of the patients who had the variation of ECG were significantly lower than those of no-complication patients.(P

The plasma levels of calcitonin gene-related peptide (CGRP) and endothelin (ET) were assayed in 76 patients with diabetic nephropathy (DN) and 23 healthy subjects, and the changes of CGRP and ET levels after treatment with angiotensin Ⅱ receptor antagonist (ARBS) were observed. The results showed that CGRP level was increased and ET level decreased after treatment with ARBS, which seemed to delay the onset and development of DN.

Objective:To investigate the priorities of each factor influencing the casualties in refugees living in border areas, so as to provide evidence for casualty forecasting in the refugees. Methods: We summarized the factors affecting the refugee casualty in the border area through searching and reviewing the related literatures. The identified factors were classified into 3 levels and the quantitative index system was established by using Delphi method, i.e. expert consulting method. The names and the meanings of each index were revised according to experts’ suggestions after 3 rounds of consulting. The weights of each index were determined by analytic hierarchy process (AHP) and comparing-reordering method. Results: A 3-level quantitative system was successfully constructed, which consisted of 4 first level indices (including natural factors, social factors, medical factors, and war factors), 12 second level indices, and 37 third level indices; the weights of all indices were determined. Conclusion: The result of our study can be used in predicting refugee casualty and provide a reference for the medical service of refugees living in the border areas.

Objective To investigate the signal pathways involved in the effects of thyroid stimulating antibody(TSAb)on the secretory function of cultured thyrocytes.Methods (1)With the method of enzyme-linked immunosorbent assay (ELISA),the PKA and PKC activities were observed.(2)Inhibitors or activators of protein kinases (PKA and PKC) were used to activate or block the signal pathways and the secretion of T3 measured by radioimmunoassay.Results (1)TSAb activated both PKA and PKC in thyrocytes in a dose-,time-dependent manner(.P.

Plasma VEGF level of T2DM was higher than that of normal control （NC, n=30） and progressively increases from normal albuminuria （NA, n=26） to microalbuminuria （MA,n=26） and to macroalbu-minuria （ODN, n=24） groups, The VEGF level was positively correlated with the levels of creatinine, HbA1c and UAER and, decreases after losartan treatment in groups of MA and ODN.