Gabapentin may bind the ? 2? subunit of the voltage -dependent calcium ion channels at the postsynaptic dorsal horn and interrupt t he series of events possibly leading to the experience of a neuropathic pain sen sation. Many clinical studies have clearly demonstrated the efficacy of gabapent in in the treatment of diabetic neuropathy and postherpetic neuralgia. Gabapenti n is considered an effective drug with its minimal side effects and drug interac tions in the management of neuropathic pain syndromes.

Although there are a number of shortcomings with these animal models, they provide important clues in understanding the underlying pathophysiology of neuropathic pain in humans. In these models, cutaneous sensory threshold of the hindlimb ipsilateral to nerve injury is measured. The presence of neuropathic pain in experimental animal models is mainly measured as allodynia or hyperalgesia, in which the normally nonnoxious or mildly noxious stimuli induce a nociceptive behavioral response. This paper mainly discusses the recent findings from the peripheral nerve injury model of neuropathic pain, as well as the different characteristics of these animal models of neuropathic pain.

AIM:To study the chemical constituents in aerial parts of Arachis hypogea L. METHODS:Compounds were isolated and purified with silica gel and Sephadex LH-20 column chromatography,and their structures were determined by using spectroscopic analysis including MS and NMR. RESULTS:Four compounds were isolated and identified as ?-sitosterol(Ⅰ)、isomedicarpin(Ⅱ)、soyasapogenol B(Ⅲ)、daucosterol(Ⅳ). CONCLUSION:Compound Ⅱ was isolated from this plant for the first time.

ObjectiveTo explore the characteristics and correlation of intelligence development and socioemotional development in 1~3 years old children with expressive language disorder,and provide a basis for early intervention.Methods125 cases with expressive language disorder(language disorder group) and 126 normal children (normal group) are evaluated in two parts (intelligence and social mood) with 0~6 years old neuropsychological development diagnosis scale and Chinese version of urban infant-toddler social and emotional assessment.Results (1)The scores in language disorder group were lower than normal group in the area of free movements (81.60±10.40 vs 89.62±7.94),adaptability (81.48±10.95 vs 91.25±8.89),language (67.46±7.20 vs 89.13±8.24),social behavior (76.61±9.73 vs 90.12±8.13) and total developmental quotient (80.17±6.39 vs 91.15±6.05) (P<0.05).(2) There were significant differences between language disorder group and normal group in the area of externalizing behavior(56.28±10.15 vs 53.57±7.91),deregulation (56.45±10.61 vs 51.11±9.32) and capabilities(46.79±9.08 vs 51.25±7.47) (P<0.05).The boys' scores in internalizing behavior were lower than the girls' (49.19±10.76 vs 54.71±9.90) (P<0.05).(3) The scores of gross motor had positive correlation with externalizing behavior (r=0.220,P<0.05).The scores of language had negative correlation with externalizing behavior and deregulation(r=-0.650,P<0.05;r=-0.470,P<0.05).The scores of social behavior had negative correlation with externalizing behavior (r=-0.208,P<0.05).There was also a negative correlation between total development and deregulation (r=-0.184,P<0.05).(4) Multiple stepwise linear regression results showed that the externalizing behavior domain and mothers' education could predict 44.1% of variances in the language area.ConclusionThe children with expressive language disorder not only show backward in level of language development,but also in the development of other areas,and also bad social behavior and emotional problems.Individualized parenting interventions should be adopted to promote these children's intelligent development in an all-round way.

Diabetic foot ulcer is a common complication of diabetes mellitus and the main cause of disability and death of diabetic patients, which has become an international medical and health problem. In recent years, the clinical usage of growth hormone in the treatment of diabetic foot ulcer has achieved good results. This article reviews the relevant literature at home and abroad, and summarizes the possible efficacy and mechanism of growth hormone in the treatment of diabetic foot ulcers.

OBJECTIVETo study the chemical constituents in the tubers of Dioscorea bulbifera.

METHODCompounds were isolated and purified with silica gel, ODS and Sephadex LH-20 column chromatography, their structures were determined by using spectroscopic methods including MS and NMR.

RESULTFourteen compounds were isolated and identified as stigmasterol (1), mono-arachidin (2), 1,7-bis-(4-hydroxyphenyl)-1E,4E,6E-heptatrien-3-one (3), behenic acid (4), demethyl batatasin IV (5), 2,3'-di-hydroxy-4',5'-dimethoxybibenzyl (6), diosbulbin B (7), diosbulbin D (8), docosyl ferulate (9), 7-bis-(4-hydroxyphenyl) -4E, 6E-heptadien-3-one (10), 5,3,4-trihydroxy-3,7-dimethoxyflavone (11), tristin(12), protocatechuic acid (13), adenosine (14).

CONCLUSIONCompounds 24, 6, 9, 10, 12, 14 were isolated from the genus Dioscorea for the first time.

Objective To evaluate the effect of ultra short-term low-dose gamma knife radiation on invasive properties of glioma cells at the genetic level.Methods Malignant glioma cell lines U87 and U251 were treated with gamma knife radiation,respectively,with a marginal doses of 0,4,6,8 and 10 Gy (all to the 80％ isodose line).Real time-PCR was used to measure the mRNA expressions of AKT-1,AKT-2,β-catenin and TCF-4 at 30 min after radiation.Results The expressions of AKT-1,AKT-2,β-catenin,TCF-4 in glioma cell lines after low-dose gamma knife radiation with a marginal dose of 6-8 Gy were significantly increased as compared with those after 0 Gy (P＜0.05); reduced expressions ofAKT-1,AKT-2,β-catenin and TCF-4 after a marginal dose of 10 Gy were found as compared with those after 0 Gy (P＜0.05); the β-catenin and AKT-1 mRNA expressions in U87 cells and TCF-4,AKT-1 and AKT-2 mRNA expressions in U251 cells enjoyed the most obvious increase at a marginal dose of 6 Gy; the TCF-4 and AKT-2 mRNA expressions in U87 cells and β-catenin mRNA expression in U251 cells enjoyed the most obvious increase at a marginal dose of 8 Gy.Conclusion Low-dose gamma knife radiation (6-8 Gy) could increase the β-catenin,TCF-4,AKT-1 and AKT-2 expressions,while high dose (10 Gy) could inhibit these effects in a short time period of 30 min.

Objective To research the effect of radiosurgery in combination of temozolomide therapy in vitro on invasion and endocrine function of rat pituitary adenoma cells.Methods Rat pituitary adenoma cell lines GH3 and MMQ were divided into control group,10 Gy irradiation group,20 Gy irradiation group,temozolomide treatment group,and radiosurgery in combination of temozolomide group;cells without exposure were used as control group,cells treated with gamma knife radiation respectively with marginal doses of 10 and 20 Gy (all to the 99％ isodose line) were used as 10 Gy irradiation group and 20 Gy irradiation group,cells treated with 250 μmol/L and 615 μmol/L temozolomide for 24 h after 20 Gy radiation were used as radiosurgery in combination of temozolomide group,and cells treated with 250 μmol/L and 615 μmol/L temozolomide for 24 h were used as temozolomide treatment group.Real time-PCR and Western blotting were employed to measure the mRNA and protein expressions of matrix metalloproteinase (MMP)-9 and vascular endothelial growth factor (VEGF);and secretion of prolactin (PRL) and growth hormone (GH) was detected by ELISA.Results As compared with the control group,the 10 Gy irradiation group and 20 Gy irradiation group had significantly lower mRNA and protein expressions of MMP-9 and VEGF,and secretion of PRL and GH (P＜0.05);and these levels in the 20 Gy irradiation group were significantly lower than those in the 10 Gy irradiation group (P＜0.05);As compared with the 10 Gy irradiation group,20 Gy irradiation group,and temozolomide treatment group,the radiosurgery in combination of temozolomide group had significantly lower mRNA and protein expressions of MMP-9 and VEGF,and secretion of PRL and GH (P＜0.05) Conclusion Gamma knife radiosurgery combined temozolomide could significantly inhibit the invasive ability and endocrine function of rat pituitary adenoma cell lines GH3 and MMQ,which has better effect than radiotherapy or temozolomide treatment alone.

Objective To investigate the curative effect of Sandplay therapy on attention deficit hyperactivity disorder (ADHD)preschool children co - morbid sleep disorder. Methods According to the random number generated by EXCEL software's RAND function,a total of 62 ADHD children co - morbid sleep disorder were randomly divi-ded into experimental group(n = 31)and control group (n = 31). The control group was treated with family behavior therapy and the experimental group was treated with sandplay therapy based on the family behavior therapy. With the Achenbach Child Behavior Check List (CBCL),Conners Parent Symptom Questionnaire (PSQ),Conner Concise Questionnaire (Conners Abbreviated Symptom Questionnaire,ASQ)and Children′s Sleep Habit Questionnaire(CSHQ),the overall curative effect was evaluated before and after treatment by their scores. Results The results of independent - samples t test showed that the scores of PSQ′s all factors,ASQ′s and CBCL′s total score,CSHQ′s total score and all factors had no significant difference between the two groups before the intervention (t conduct problem = - 0. 847,t learning problems =0. 504,t psychosomatic disorder = - 1. 008,t hyperactivity/ impulsion = - 0. 331,t anxiety = 0. 690,t hyperactivity index = 0. 757,t CBCL′s total score =- 0. 532,t ASQ′s total score = 0. 514,t CSHQ′s total score = 1. 132,t sleep impedance = - 0. 767,t sleep latency = - 0. 634,t sleep duration = 0. 150, tsleep anxiety = 0. 149,t sleep wake = 0. 417,t sleep disorder = 0. 709,t sleep apnea = 0. 950,t daytime sleepiness = 1. 530,all P > 0. 05). The scores of PSQ′s all factors,ASQ′s and CBCL′s total score,sleep impedance,sleep latency,sleep duration,sleep wake, daytime sleepiness and CSHQ′s total score all had significant differences between the two groups after the intervention(t conduct problem = - 3. 982,t learning problems = - 2. 258,t psychosomatic disorder = - 2. 470,t hyperactivity/ impulsion = - 4. 377,t anxiety =- 2. 684,t hyperactivity index = - 4. 249,t CBCL′s total score = - 3. 982,t ASQ′s total score = - 5. 576,t sleep impedance = - 3. 712,t sleep latency =- 2. 947,t sleep duration = - 2. 332,t sleep wake = - 6. 031,t daytime sleepiness = - 6. 256,t CSHQ′s total score = - 6. 706,all P < 0. 05). The results of analysis of variance of repeated measurement showed that PSQ behaviour problem,psychosomatic disor-der,hyperactivity and hyperactivity index scores of the main effects of intervention had significant difference (F conduct problem = 4. 966,F psychosomatic disorder = 5. 176,F hyperactivity/ impulsion = 4. 949,F hyperactivity index = 4. 926,all P < 0. 05);the main intervention effects of ASQ and CBCL′s total score had statistical difference (F CBCL = 4. 398,F ASQ = 4. 814,all P < 0. 05);the main intervention effects of sleep impedance,sleep duration,sleep wake and CSHQ′s total score had statistical difference (F sleep impedance = 5. 697,F sleep duration = 4. 467,F sleep wake = 6. 610,F CSHQ′s total score = 6. 016,all P <0. 05). There were significant differences in the characteristics of the initial sand table and the final sand table in the experimental group [traumatic subject:(4. 48 ± 1. 55)cases vs. (1. 23 ± 0. 81)cases,t = 8. 247;curing subject:(1. 77 ± 1. 09)cases vs. (4. 55 ± 1. 46)cases,t = 11. 274,all P < 0. 05]. Conclusion Sandplay therapy can effectively improve the core symptoms of preschool ADHD children co - morbid sleep disorders,which can be used as an early in-tervention.