OBJECTIVE:To discuss the way to raise the market concentration ratio of pharmaceutical industry in China. METHODS:The present situations of market concentration ratios home and abroad were analysed and compared.RESULTS&CONCLUSION:The market concentration ratio of pharmaceutical industry was lower in China than that in foreign coun?tries.In order to raise the market concentration ratio of pharmaceutical industry in China,we should heighten the standard of market access,acquire,merge and procure existing pharmaceutical enterprises and lower the barrier to withdraw from market.

OBJECTIVE:To study the capital requirements of pharmaceutical industry in China by using the theory of entry barriers.METHODS:The idea of requiste capital barriers in the theory of barriers was introduced and standardized.The level of capital requirements in Chinese pharmaceutical industry was calculated by the method of the survival of the fittest.RESULTS&CONCLUSION:The level of the capital requirements of the pharmaceutical industry in China is100million RMB of current value.

OBJECTIVE:To provide references and suggestions for the further development of pharmaceutical industry in Hangzhou city.METHODS:Using the classic normal form of SCP in the industry economics,i.e.market structure-the manufacturer behavior-market performance,a transverse and longitudinal comparative study was carried out on the market structure,the manufacturer behavior and the market performance of the Hangzhou's pharmaceutical industry between 2003 and 2006.RESULTS:Hangzhou's pharmaceutical industry was characterized by oligarch model in concentration degree,low differentiation in products;high barrier of entry and exit;low strength in advertisement,and rational in the merge and recombination.The manufacturers in this area attached increasingly importance to technological progress and the market performance was remarkable.CONCLUSIONS:Considering the status quo of Hangzhou's pharmaceutical industry,it is advisable for the manufacturers enlarge and strengthen their business,enhance its R&D investment and product differentiation,and stick to the market-oriented marketing concept.

The levels of urinary neopterin in 30 patients with SLE were determined by reversed-phase high performance liquid chromatgraphy(HPLC). The results showed: (1 ) the levels of urinary neopterin in 30 patients with SLE were significantly higher than those in 20 controls. (2) The concentrations of urinary neopterin in SLE patients were significantly correlated with the disease activity, they decreased after efficacious treatment with reduced disease activity. (3) There was significant positive correlation between urinary neopterin concentration and erythrocyte sedimentation rate, but there were no statistical relations between urinary neopterin levels and ANA, antibody to ds-DNA, or plasma C3 levels. These results indicate that urinary neopterin may be used as an index for the assessment of disease activity in patients with SLE and monitoring therapeutic effect.

OBJECTIVE:To discuss the barriers to entry of pharmaceutical industry of our country into international market.METHODS:Directed by the theory of the barriers to entry of the industry organization theory,the barriers to entry into the international pharmaceutical industry was ordered by using the methods of the excess profit,the enterprise numbers,and the proportion of enterprise scale.RESULTS:The barriers to entry into the international chemical pharmaceutical industry and the medical instrument industry are the highest in the sequence of pharmaceutical industry.CONCLUSION:At the time that the industry of our country is reforming,we should regulate and direct the pharmaceutical industry right by using the theory of the industry organization.

Objective To investigate the relationship between hepatitis B virus pre-core region 1896 site mutation in cases of chronic hepatitis B and IFN-alpha treatment.Methods 200 patients with chronic hepatitis B were randomly divided into experimental group(n=100) and control group(n=100).The patients in experimental group were treated with regular liver-protective drugs and IFN-alpha,while Control group patients received only regular liver-protective drugs for 6 months.PCR-RFLP were used to detect hepatitis B virus(HBV)DNA with pre-C region 1896 site mutation in two grops pre and post treatment.Results The mutation rates before interferon treatment were 20%(20/100) and 18%(18/100) in experimental group and control group,respectly.After treatment,the effective rates in experimental group were 60%(12/20) and 50%(40/80) in Patients with Chronic Hepatitis B variant and wild strain virus Infection,respectly.The effective rate in experimental group was significantly higher than that in control group.Conclusion IFN-alpha therapy was not correlated with genomic variability of the core region.

Objective To study the chemotactic activity of T lymphocytes from patients with psoriasis to proinflammatory cytokines in vitro and its role in the pathogenesis in psoriasis. Methods A 48 microchemotaxis chamber was employed to determine T cell chemotactic activity. In addition, the expression of T cell activation markers such as HLA DR and interleukin 2 receptor was analysed with fluorescence activated cell sorting technique and serum IL 8 level was measured with ELISA. 45 patients with psoriasis (23 patients with severe psoriasis and 22 patients with mild psoriasis) and 21 patients with atopic dermatitis were investigated, and 20 healthy controls were tested equally. Results ①T cell chemotactic responses were significantly decreased in patients with severe psoriasis and atopic dermatitis as compared to healthy controls. ②Increased expression of activation markers such as HLA DR and interleukin 2 receptor was demonstrated in circulating T cells from severe psoriatic patients and atopic dermatitis patients in comparison to healthy controls. ③Serum IL 8 level was significantly increased in patients with psoriasis and atopic dermatitis. Conclusion The in vitro chemotactic response of circulating T cells in patients with severe psoriasis to IL 8 is significantly impaired, furthermore, the in vivo activation state of T lymphocytes in these patients and increased level of serum IL 8 seem to be associated to the decreased in vitro T cell chemotactic response.

Objective To study the relationship between the in situ expression of infiltrating T lymphocyte subsets and leukocyte adhesion molecules in psoriatic patients. Methods Immunohistochemical technique was employed to study the expression of infiltrating T lymphocyte subsets (CD3, CD4, CD8) and leukocyte adhesion molecules (ICAM 1, ELAM 1, VCAM 1) in the lesional and non lesional psoriatic skin. Results There was a significant increase of T lymphocyte subsets (CD3, CD4 and CD8) and adhesion molecules (ICAM 1, ELAM 1, VCAM 1) in the lesional skin than those in non lesional skin in psoriasis, and that there is a significant positive correlation between the density of infiltrating T lymphocyte subsets and the intensity of in situ expression of adhesion molecules. In comparison to the materials from normal controls, the upregulation expression of adhesion molecules was observed in non lesional skin and normal appearance skin after short duration topical treatment with corticosteroid. Conclusion Increased upregulation expression of adhesion molecules is closely associated with the infiltration of T lymphocytes in lesional skin of psoriasis and may be one of the factors for the recurrence of psoriasis.

Objective To address the question whether circulating T cells from the patients with primary and metastatic malignant melanoma show altered chemotaxis to monocyte chemotactic protein 1 (MCP 1) and its relation to tumor infiltrating lymphocyte (TIL) and metastasis. Methods Chemotactic responsiveness of T cells towards MCP 1 and immuno histochemistry study were investigated in patients with primary and metastatic melanoma compared to patients with basal cell carcinoma and healthy persons. Results T cells from primary and metastatic melanoma patients showed a significantly decreased chemotactic migration towards MCP 1 and that T cells from patients with basal cell carcinoma showed normal chemotactic response. Immuno histochemistry study showed that there was no correlation between the density of TIL and the decreased chemotaxis of circulating T cells to MCP 1 in patients with primary melanoma. Conclusion Circulating T cells from patients with primary and metastatic malignant melanoma show a MCP 1 specific decrease in chemotactic migration, this may be due to abnormal expression or modulation of MCP 1 receptor on these cells.

Objective To investigate the relationship between keratinocyte growth factor (KGF), nerve growth factor(NGF)and psoriasis;and to study the interaction between keratinocytes and interstitial cells in psoriasis. Methods The expression of KGF, KGF receptor (KGFR), NGF, NGF receptor (NGFR), epidermal growth factor receptor (EGFR) and proliferating cell neuclear antigen (PCNA) was studied with immunohistochemical technique (SP) in lesional skin, non lesional skin and normal controls. Results Significant overexpression of KGF and KGFR was present in psoriatic basal cell layers and suprabasal cell layers compared to that in non lesional and normal controls (P0.05). Expression of KGF and KGFR was absent in dermis. Expression of NGF and NGFR was observed mainly in granular cell layers and upper spinous layers, and was significantly different between normal controls and non lesional, between non lesional and lesional skin (P