OBJECTIVETo explore the feasibility of preparation of a mouse model of orthotopic colon cancer by injecting tumor cell suspension into mesenteric triangle of the cecum.

METHODSTwenty SPF 8-week old BALB/c mice (male:female = 1:1) were used in this study. The mouse caecum was exposed by laparostomy, and suspension of mouse colon adenocarcinoma CT26. WT cells was injected into the mesenteric triangle of cecum for preparation of a mouse model of orthotopic colon cancer.

RESULTSMouse orthotopic colon cancer was developed by injection of tumor cell suspension into mesenteric triangle of the cecum showing a successful rate of 100%, without intestinal obstruction, and the liver, spleen, diaphragm and mesenteric lymph nodes metastasis rates were high in all the 20 experimental mice.

CONCLUSIONSThe establishment of mouse models of orthotopic colon cancer by injection of tumor cell suspension into the mesenteric triangle is a simple, rapid, and easy to master procedure, causing less damage to the colon wall, safe and with less trauma to the mice. This method may provide an ideal mouse model of orthotopic colon cancer for the study of pathogenesis as well as liver metastasis mechanisms of colon cancer.

Adenocarcinoma ; pathology ; secondary ; Animals ; Cecal Neoplasms ; pathology ; Cecum ; Colonic Neoplasms ; pathology ; Disease Models, Animal ; Feasibility Studies ; Female ; Liver Neoplasms ; secondary ; Lymphatic Metastasis ; Male ; Mice ; Mice, Inbred BALB C ; Neoplasm Transplantation ; methods

Objective:To explore the effects of mindfulness-based stress reduction training (MBSRT) on anxiety and depression levels and sleep quality of clinical nurses.Methods:Totally 90 clinical nurses from the Second Hospital of Harbin Medical University were selected by convenient sampling from April 2018 to June 2019, and were divided into experimental and control groups according to the order of enrollment, with 45 cases in each group. Nurses in the control group received routine sleep and emotional guidance, while nurses in the experimental group underwent MBSR training on this basis. Self-Anxiety Scale (SAS) , Self-Depression Scale (SDS) , and Pittsburgh Sleep Quality Index (PSQI) were used to evaluate the intervention effect in both groups 1 day before the intervention and the day when the intervention was completed. Finally, 44 nurses in the experiment group and 45 in the control group completed the study.Results:Before the MBSRT intervention, there was no statistically significant difference in the SAS, SDS, and PSQI scores between the two groups of nurses ( P>0.05) . After the intervention, the SAS and SDS scores of nurses in the experimental group were lower than those of the control group, and the differences were statistically significant ( t=-3.117, -3.498; P<0.05) ; the total PSQI score and scores in such dimensions as subjective sleep quality, sleep latency, sleep continuity, sleep efficiency, sleep disorder, hypnotic drug use, and daytime dysfunction in the experimental group were lower than those of the control group, and all differences were statistically significant ( P<0.05) . Conclusions:MBSRT can effectively reduce the level of anxiety and depression and improve the sleep quality of clinical nurses.

Objective:To explore the effect of network learning environment on critical thinking ability of nursing undergraduates.Methods:From September 2021 to January 2022, a total of 84 nursing undergraduates in the third grade of Nursing School of Harbin Medical University were selected as the research objects. The Online Learning Environment Scale and the Chinese version of Critical Thinking Ability Scale were used to investigate.Results:The total score of Online Learning Environment Scale of 84 nursing undergraduates was (63.21±15.29) , and the Chinese version of Crital Thinking Ability Scale was (248.71±36.35) . The total score of Online Learning Environment Scale of nursing undergraduates was positively correlated with the total score of Chinese version of Critical Thinking Ability Scale of nursing undergraduates ( P<0.01) . The results of multiple linear regression analysis showed that the influencing factors of critical thinking ability of nursing undergraduates included family economic situation, whether they could receive free online database resources from schools, and online learning environment ( P<0.05) . Conclusions:The online learning environment for nursing undergraduates is at a moderate level. It is suggested that nursing undergraduates should optimize the content and form of online learning and provide targeted learning support resources so as to improve the critical thinking ability of nursing undergraduates.

Objective:To investigate the status quo and influencing factors of the somatization of depressive emotions among college students, in order to provide a theoretical basis for the reform of mental health education and the formulation of operable mental health counseling for college students.Methods:Totally 297 college students from two institutions of higher education in Heilongjiang Province were selected by convenience sampling and investigated with the College Student Depressive Emotion Somatization Questionnaire, Attitudes Toward Seeking Professional Psychological Help Scale-Short Form (ATSPPH-SF), and the Type D Personality Scale (DS14) .Results:The total score for the College Student Depressive Emotion Somatization Questionnaire among the 297 students was (28.49±8.07), and the total score for the ATSPPH-SF was (21.98±2.73). Multiple linear regression analysis showed that the influencing factors for the somatization of depressive emotions in college students were professional psychological help-seeking attitudes and whether they had a Type D personality ( P < 0.05) . Conclusions:During university education, there should be a focus on students' personality traits and the positivity of their attitudes toward seeking professional psychological help in order to mitigate the occurrence of adverse psychological issues such as symptoms of depressive somatization.

Objective To explore the feasibility of preparation of a mouse model of orthotopic colon cancer by injecting tumor cell suspension into mesenteric triangle of the cecum. Methods Twenty SPF 8?week old BALB/c mice ( male ∶ female=1 ∶ 1) were used in this study. The mouse caecum was exposed by laparostomy, and suspension of mouse colon adenocarcinoma CT26.WT cells was injected into the mesenteric triangle of cecum for preparation of a mouse model oforthotopic colon cancer. Results Mouse orthotopic colon cancer was developed by injection of tumor cell suspension into mesenteric triangle of the cecum showing a successful rate of 100%, without intestinal obstruction, and the liver, spleen, diaphragm and mesenteric lymph nodes metastasis rates were high in all the 20 experimental mice. Conclusions The establishment of mouse models of orthotopic colon cancer by injection of tumor cell suspension into the mesenteric triangle is a simple, rapid, and easy to master procedure, causing less damage to the colon wall, safe and with less trauma to the mice. This method may provide an ideal mouse model of orthotopic colon cancer for the study of pathogenesis as well as liver metastasis mechanisms of colon cancer.

Objective To explore the feasibility of preparation of a mouse model of orthotopic colon cancer by injecting tumor cell suspension into mesenteric triangle of the cecum. Methods Twenty SPF 8?week old BALB/c mice ( male ∶ female=1 ∶ 1) were used in this study. The mouse caecum was exposed by laparostomy, and suspension of mouse colon adenocarcinoma CT26.WT cells was injected into the mesenteric triangle of cecum for preparation of a mouse model oforthotopic colon cancer. Results Mouse orthotopic colon cancer was developed by injection of tumor cell suspension into mesenteric triangle of the cecum showing a successful rate of 100%, without intestinal obstruction, and the liver, spleen, diaphragm and mesenteric lymph nodes metastasis rates were high in all the 20 experimental mice. Conclusions The establishment of mouse models of orthotopic colon cancer by injection of tumor cell suspension into the mesenteric triangle is a simple, rapid, and easy to master procedure, causing less damage to the colon wall, safe and with less trauma to the mice. This method may provide an ideal mouse model of orthotopic colon cancer for the study of pathogenesis as well as liver metastasis mechanisms of colon cancer.