Objective To observe the clinical effects and complications of coronary intervention through radial artery and femoral artery.Methods A hundred and sixty patients received coronary intervention treatment or coronary angiogram in the Second Affiliated Hospital of Bengbu Medical College from March 2010 to March 2015 were enrolled in this study.The patients were divided into radial artery puncture group (n =80),who received radial artery puncture and femoral artery puncture group (n =80) who received femoral artery puncture.The general date such as puncture time,intervention success rate,time in bed,postpuncture compression time and complications such as postoperative bleeding,arterial occlusion and postoperative untoward reactions were recorded and compared.Results The intervention success rate of radial artery puncture group was 90.0％ lower than that of femoral artery puncture group (97.5％),with statistically significant differences (P＜0.05).The puncture time,postpuncture compression time,time in bed and hospital stays of radial artery puncture group were significantly shorter than those of femoral artery puncture group (P ＜0.01).As for complications,the incidences of postpuncture bleeding and vagus nerve reflex in radial artery puncture group were significantly lower than that in femoral artery puncture group (P＜0.05).As for postoperative untoward reactions,the incidences of irritable and insomnia,uroschesis,pain caused by puncture,local skin injure,back pain in radial artery puncture group were significantly lower than those in femoral artery puncture group (P＜0.01).Conclusion With the advantages of mini-invasion,such as shorter time in bed and hospital stays,lower complications and untoward reactions rates,coronary intervention through radial artery is worth of clinical application.

Connection between the superior colliculus(SC)and the dorsal and ventral lateral geniculate nuclei (LGd and LGv) were studied in adult golden hamsters using anterograde and retrograde pathway tracing methods 1. In four hamsters, the cytoarchitecture of the SC, LGd and LGv was examined in brain sections stained with Nissl's or Loyez's method. 2. A single injection of a mixture of ~3H-leucine and ~3H-proline was made into various regions of the SC in each of 4 animals (1 day survival) for studying the pattern of terminal distribution of the projections from the SC to the ipsilateral LGd and LGv. In animals where the injection site was located in the superficial gray layers of the lateral portion of the SC, labelled terminals were found in the caudal lateral part of the LGd and LGv. In the case where the injection was restricted in more medial part of the SC, the terminal labelling was observed in the rostral lateral portion of the lateral geniculate nuclei. 3. One day after an injection of horseradish peroxidase (HRP) into the LGd (4 animals), LGv(2 animals), or LP(2 animals) labelled neurons were observed in the superficial gray layers of the ipsilateral superior colliculus. No HRP-labelled neuron was detected in deeper laminae in the colliculus. These results indicate that neurons in the superficial gray layers of the SC project to the ipsilateral LGd and LGv, and the projections are organized topogra- phically according to the retinotopic or visual field map

Objective To detect 10 RNA viruses including Alphavirus in Togoviridae, Flavivirus in Flaviridae, Hantavirus and Nairovirus in Bunyavirudae and SARS-CoV in Coronavirudae by using genechip technique. Methods The universal PCR primers of Alphavirus and Flavivirus and the PCR primers specific for HFRSV in Hantavirus and XJHFV in Nairovirus were designed by DNAStar software. PCR primers specific for SARS-CoV were adopted from WHO website. In addition, all the PCR primers specific for each virus were designed inside the regions of universal primers. These specific primers were utilized for amplification of cDNA probes. The concentration of probes, the hybridization temperature and duration, the formulation of hybridization solution and the washing conditions were optimized. Results The specific hybridization signals could be obtained when the concentration of probes was 0.3?g/?l. Good hybridization signals could be obtained for all the 10 RNA viruses when the hybridization solution contained 20% formamide, and the hybridization reaction was conducted at 60℃ for 1.5 hours. Two or four pathogens could be detected simultaneously when the target nuclear acids were amplified by multiplex PCR. Conclusion The results showed that the virus pathogens could be detected by genechip technique, and the key step was to design suitable primers and probes.

Leptin,the product of the obese gene,is a protein produced and secreted primarily by adipose tissue.Leptin initiates several biological effects by binding to its receptors.Many studies have shown that leptin can reduce fat accumulation,but its mechanism is still unclear.This article reviewed the effects of leptin on lipogenesis,and especially on proliferation and differentiation of preadipocytes.

Objective To explore the expression of the nuclear factor -κB (NF-κB) regulating monocyte ehemoactracttive peptide -1 ( MCP-1) in asthma patients, and investigate the effect of seretide, budesonide and vento-lin. Methods 81 asthma patients were randomly divided into seretide therapy group and budesonide and ventolin therapy group. The NF-κB activation in PBMC and the plasma concentrations of MCP-1 were measured by ELISA. ventolin therapy group (1.70±0.39) ng/L of asthma patients were significantly higher than that control group (0.89±0.34) ng/L ( P<0.001 ) ; The plasma MCP-1 level in seretide therapy group ( 66.89±5.62 ) ng/L and in budesonide and ventolin therapy group (73.35±7.52 ) ng/L of asthma patients were also significantly higher than level of seretide therapy group in asthma patients were significantly lower than budesonide and ventolin therapy group (P <0.001 ). Conclusion NF-κB gene and its regulating protein MCP-1 may be involved in asthma. Glucocotios-teroid and beta2 agonists combination may prolong the process of asthma.

Objective To investigate the clinical characteristics and the treatment of pancreatic encephalopathy (PE) and Wernicke's encephalopathy (WE). Method A retrospective study was conducted on 596 cases of acute pancreatitis. Results There were 93 cases of severe acute pancreatitis (SAP),among them encephalopathy was discovered in 10 patients (1.7%). All 6 patients of PE developed in SAP (6.5%);3 patients died (3% of SAP,50% of PE). Four cases of WE developed in AP (0.7%);2 patients died (0.3% of AP,50% of WE). Two patients of WE were treated with parenteral thiamine (vitamin B_ 1 ),and they survived. Conclusions PE occurred in early stage of SAP or recrudescence,while WE usually occurred in convalescent stage of SAP/AP. Long fasting,repeated vomiting,and total parenteral nutrition (TPN) without VitB_ 1 were main causes of VitB_ 1 deficiency,which might be the main causative factor in WE.

Objective To compare the effects of patient's tolerance to laryngeal mask airway (LMA) and tracheal tube (TT) on the appropriate level of sevoflurane anesthesia.Methods Eighty ASA Ⅰ or Ⅱ patients aged 30-60 yr weighing 50-70 kg undergoing elective thyroid or breast surgery were randomly divided into 2 groups (n=40 each):LMA group and TT group.Anesthesia wag induced with propofol 1.6mg/kg,fentanyl 3μg/kg and recuronium 0.6mg/kg.LMA or tracheal tube was inserted,and the patients were mechanically ventilated.Anesthesia was maintained with inhalation of 1.5%-3.0% sevoflurane and 50% N2O in O2 in group LMA,and with 2.5%-5.0% sevoflurane and 50% N2O in O2 in group TT.The flow of O2 and N2O was 0.7-1.0L/min,and the concentration of sevofluranee was adjusted to maintain AAI at 15-25.ECG,HR,MAP,SpO2,PETCO2,AEP and end-tidal sevoflurane concentration were continuonsly monitored.The end-tidal sevoflurane concentration was recorded at 1,5 and 10 min after LMA/TT was placed (T1-3),at 1h after skin incision (T4) and at the end of operation before removal of LMA or extubation (T5).The recovery time of consciousness,adverse cardiovascular events and adverse reactions were recorded.Results The end-tidal sevoflurane concentration was significantly lower,the recovery time of consciousness and removal of LMA or extubation time were shorter,and the incidence of adverse cardiovascular events and adverse reactions was lower in LMA group than in TT group.Conclusion At the same depth of anesthesia (AAI 15-25),sevoflurane concentration is significantly lower in LMA group than in TT group,with fewer complications and smaller cardiovascular reaction.

Objective To observe the effects of leptin on the proliferation and differentiation of human preadipocyte in vitro, and to explore the possible mechanism of the generation of obesity regulated by leptin. Methods The human preadipocytes were isolated from human subcutaneous adipose tissue of abdomen and cultured in vitro. The effects of leptin (0-1 000 ng/ml) on the proliferation, lipid accumulation and the mRNA expression of PPAR-γ2 and C/EBP-α, which are the differentiation and transcription factor of human preadipocyte, were analyzed by the methods of MTT, cell counting, extracting stained intracytoplasmic lipid with oil red O and RT-PCR. Results Leptin (1 000 ng/ml) could stimulate the proliferation, lipid accumulation and the mRNA expression of PPAR-γ2 and C/EBP-α (P<0. 05). There were not obvious effects on the proliferation and lipid accumulation in the groups of lower (10 ng/ml) and common (100 ng/ml) concentration (P>0. 05). Conclusion Leptin in higher concentration can stimulate the proliferation and differentiation of preadipocye in vitro, which indicates that leptin may regulate the generation of obesity through acting on the proliferation and differentiation of preadipocyte at the pathologic state of leptin resistance and high leptin concentration in serum.

Objective To investigate the efficacy of uterine artery chemoembolization in the treatment of locally advanced cervical cancer.Methods A total of 268 patients with locally advanced cervical cancer were treated with uterine artery chemoembolization in our department.The stage distribution among the patients included 132 stage Ⅰ B2,85 stage Ⅱ A1 and 51 stage Ⅱ A2.There were 223 patients of squamous cell carcinoma,24 patients of adenocarcinoma,9 patients of adenosquamous carcinoma,small cell carcinoma of the 7 patients,5 patients of neuroendocrine carcinoma.Transcatheter uterine artery infusion of paclitaxel and nedaplatin,gelatin sponge particles was applied for uterine artery embolization.The clinicopathological parameters were analyzed,and their impacts on tumor response were investigated.RECIST criteria were used to evaluate the response in solid tumors.Student t test was used to compare cervical tumor diameter before and after treatment,and Chi-square test was used for comparison of categorical data.Follow up examinations included pelvic ultrasound,gynecology,vaginal stump cell smears.Results Of the 268 patients,74 (27.6％) patients showed a complete response,160 (59.7％) patients had a partial response to uterine artery chemoembolization,and the overall response rate was 87.3％.A total of 258 (96.3 ％) patients underwent surgery,and pathological complete response were identified in 46 (17.2％).Forty (14.9％) patients were found to have lymph node metastasis after surgery.Response rates of stage Ⅰ B2 and Ⅱ A cases were 94.7％ and 80.1％,respectively,P ＜ 0.05.Patients with squamous cell carcinoma showed a better response rate than patents with other pathological types (94.2％ vs.53.3％),P ＜ 0.05.Initial tumor volume and cycles of preoperative uterine artery chemoembolization had no effect onthe response rate.Conclusions Uterine artery chemoembolization can increase the rate of surgical resectionof patients with locally advanced cervical cancer and can improve the reaction rate with tolerable side effect.It is an applicable option of treatment for patients with locally advanced cervical cancer in the neoadjuvanttreatment.

Objective To evaluate the value of ultrasonic quantitative method in the diagnosis of liver fibrosis in chronic hepatitis B (CHB) patients. Methods Ultrasonography was performed in 186 CHB patients who underwent liver biopsies. Fifteen indices including liver capsule thickness and fourteen texture parameters of gray level co-occurrence matrix were extracted from standard sonograms and compared with fibrosis stages by histopathology. The status of liver fibrosis was divided into five stages from S0 to S4 by histopathology based on the disease severity. ANOVA and Spearman correlation analysis were applied to analyze the differences and relationships between these indices and pathological stage, respectively. Then discriminant analysis models were established based on the indices for quantitative diagnosis of liver fibrosis. Results Among the fifteen indices, including liver capsule thickness, only the variance (F=0. 55, r=0. 06; both P＞0. 05), sum average (F=0.61, both r=0.05 ; P＞0.05), sum entropy (F=1.68, r=0.09; both P≥0.05) and entropy (F=1.39,r=0.12; both P＞0.05) were not significantly associated with the stages and not manifested linear correlation. Using biopsy results as gold standard, the correct rank rate of discriminant analysis model analysis in the patients staged from S0 to S4 were 80. 0%, 64. 9%, 61.3%, 74. 1% and 80.6 %, respectively. There were 73.1% of cross-validated cases who were accurately classified by the model analysis. The sensitivity, specificity and accuracy in patients with stage ≥ 1 were 97. 6%,80.0% and 91.9%, respectively; those in patients with stage≥2 were 92.1%, 89.7% and 90.9%,respectively; those in patients with stage≥3 were 94.8%, 96.1% and 95.7%; and those in patients with stage 4 were 80. 6%, 97.4 % and 94.6%, respectively. When considered S0 as no fibrosis, S1 as mild fibrosis, S2 and S3 as moderate to severe fibrosis and S4 as early cirrhosis, the consistence rates between discriminant analysis model and biopsy result were 81.7%, 78. 4%, 56. 9% and 90.3%,respectively. There were 74.7% of cross-validated cases who were correctly classified. The sensitivity, specificity and accuracy of the models for determining the fibrosis severity in patients≥mild fibrosis were 97.6%, 81.7% and 92.5%, respectively; those in patients ≥ moderate to severe fibrosis were 83. 1%, 94.8% and 89.2%, respectively; those in patients with early cirrhosis were 90.3%, 93.5% and 93.0%, respectively. Conclusion As a novel and noninvasive method, ultrasonic texture analysis could quantitatively determine liver fibrosis in CHB patients and is worthy of further investigation.