Objective To investigate the effect of Celebrex on hemorheology and levels of serum intercelluar adhesion molecule-1 (sICAM-1),serum vascular cell adhesion molecule-1 (sVCAM-1),tumour necrosis factor-α (TNF-α),interleukin-6 (IL-6) in patients with delayed fracture healing.Methods 201 patients with delayed fracture healing in our hospital were randomly divided into observation group (n =101) and control group (n =100).The patients in control group received conventional surgical internal fixation,while patients in observation group received conventional surgical internal fixation combined with Celebrex.The clinical efficacy was observed,while the changes of hemorheology and levels of sICAM-1,sVCAM-1,TNF-α,IL-6 were compared between two groups before and after treatment.Results The effective rate of observation group was 94.1％,which was significant higher than that of control group 70.0％ (P＜0.01).There were no significantly differences between two groups in the indexes of hemorheology and levels of sICAM-1,sVCAM-1,TNF-α,IL-6 before treatment (P＞0.05).The indexes of hemorheology and levels of sICAM-1,sVCAM-1,TNF-α,IL-6 were significantly reduced after treatment,while the decreased degrees of observation group were better than those of control group,with statistically significant difference (P＜0.05).Conclusions Celebrex can improve the indexes of hemorheology,reduce the levels of sICAM-1,sVCAM-1,TNF-α,IL-6 and improve the clinical effect in treatment of delayed fracture healing.It is worth of popularization and application.

Objective To investigate the effect of p 38 mitogen-activated protein kinase ( MARK) on expression of matrix metalloproteinase-9(MMP-9) and formation of cerebral edema in rats with cerebral ischemia-reperfusion. Methods Fifty-four SPF male SD rats were randomly divided into sham operation group ( Sham group ) , ischemia-reperfusion group(I/R group) and p38 inhibitor group(SB group).Middle cerebral artery ischemia-reperfusion model was made by modified line plug method .The neurologic exams were assessed at 24 h after reperfusion , Evans Blue method was used tomeasurement Blood-brain barrier`s permeability,the wet-dry ratio was used to measured to estimate cerebral edema ,Western blot was used to detect the expression of phosphorylation p 38 ( p-p38 ) and MMP-9 in the infarct region .Results Compared with Sham group , I/R group had aggravated neurological deficits ( P<0.05 );compared with the I/R group, SB group had alleviate neurological deficits (P<0.05).Compared with Sham group, blood-brain barrier permeability and brain water content of I /R group were significantly increased ( all P<0.05 );compared with the I/R group, blood-brain barrier permeability and brain water content of SB group were significantly decreased(all P<0.05).Compared with Sham group , the expression of p-p38, MMP-9 in margin of ischemia of I/R group were significantly increased (all P<0.05);compared with the I/R group, the expression of p-p38, MMP-9 in margin of ischemia of SB group were significantly decreased (all P<0.05).Conclusions p38 MAPK involved in the formation of brain edema in rats with cerebral ischemia-reperfusion , and its mechanism may be cerebral ischemia-reperfusion activates p38MAPK,and it make the expression of MMP-9 in margin of ischemia up-regulated,damaging blood-brain barrier permeability ,leading to cerebral edema .

Objective To observe the contrast sevoflurane anesthesia and propofol intravenous anesthesia in children hernia surgery anesthesia on blood flow dynamics in children to assess the quality of anesthesia, anesthesia program aims for the future choose to provide important reference for basis . Methods The surgical treatment of pediatric hernia patients from January 2012 to June 2016 was prospectively selected as the study group, and the rats were divided into two groups by random drawing method. Results There were other children point and T1 relatively SBP, DBP were significantly lower (P<0.05), the observation group were significantly lower than the control group (P<0.05), the control group of children with T2, T3 compared with T1 point HR significantly increased (P<0.05). Conclusion Pediatric hernia surgery using laryngeal mask sevoflurane anesthesia exact effect, maintaining hemodynamic stability, safety, and can be widely used in clinical.

Objective To explore the clinical efficacy of low-dose cytarabine and harringtonine (LD-HA) regimen in the induction therapy of acute myeloid leukemia (AML) except M3. Methods 52 AML patients who received LD-HA were analyzed retrospectively. The patients were graded according to molecular biological and cytogenetic risk degree. The clinical efficacy, toxicity of LD-HA and long-term survival followed-up were compared with those of idarubicin and cytarabine (IA) regimen in 49 patients. Results After one cycle, the overall remission (OR) rates of LD-HA group and IA group were 71.2%(37/52) [CR rate 50.0%(26/52), PR rate 21.2%(11/52)] and 53.1%(26/49) [CR rate 44.9%(22/49), PR rate 8.2%(4/49)], respectively, with no statistical significance of OR between the two groups (P= 0.068). OR rates were not statistically significant in either low-risk group or intermediate-risk group between LD-HA group and IA group (P> 0.05), but OR of high-risk group in LD-HA was much higher than that in IA group [100 % (11/11) vs 66.7 % (12/18), P<0.05]. Cardiac toxicity and bone marrow suppression in LD-HA group were much milder than those in IA group. The patients unfit for standard chemotherapy could tolerate to LD-HA regimen. Conclusions LD-HA regimen as induction for high risk AML patients can improve the OR rate, and reduce the side effects, which is beneficial for high-risk AML patients.

Objective To evaluate the diagnostic approaches and influencing factors of prognosis after repeated hepatectomy for recurrent liver cancer. Methods Fifty seven cases of recurrent liver cancer underwent surgical resection. The disease free survival, cumulative survival and possible influencing factors of prognosis were studied. Results The 1-, 3-, 5- and 10-year disease free survival rates after first resection were 63.9%, 38.3%, 26.6% and 12.8%, respectively. The 1-, 3-, 5- and 10-year survival rates of resection for recurrent liver cancer were 56.6%, 37.7%, 31.9% and 16.2% and the 1-, 3-, 5- and 10-year cumulative survival rates were 82.1%, 60.85, 47.6% and 19.5%, respectively. The influencing factors of prognosis after repeated hepatectomy for recurrent liver cancer size, number of nodules, tumor-free duration and weather when the patients underwent curative resection. Conclusions For early detection of recurrent liver cancer, AFP test, sonography each month and computed tomography every 3 months should be conducted for patients after the first hepatectomy. For the recurrent liver cancer, surgical resection is an effective treating measure when it is possible. The influencing factors of prognosis after repeated hepatectomy for recurrent liver cancer are tumor size, number of nodules, tumor-free duration, tumor capsule and manner of repeated hepatectomy.

Objective:To investigate the localization and identification value of high frequency ultrasound in displaying connection nerves of SNT, and to improve the accuracy of ultrasound diagnosis.Methods: Retrospective analysis the results of high frequency ultrasound to 49 cases proved by pathology annually patients (54 tumors, 54 connection nerves), and compared with the results of pathological diagnosis, evaluating the accuracy of high frequency ultrasound in displaying connection nerves. Results: The longitudinal section appearance of tumor connection nerve showed hypoechoic band and parallel fine belt-like structure surrounded by hyperechoic lines, it was enlarged gradually and became triangular close to the tumor. To the distribution of sex, age and course of the disease, the rates of high frequency ultrasound in displaying connection nerves were no significant difference (x2=0.083,x2=0.454,x2=1.277;P>0.05). The rate of high frequency ultrasound in displaying connection nerves of SNT was 94.4%, compared with the results of pathological diagnosis, the distribution ratio and the rate of high frequency ultrasound in displaying connection nerves showed no significant difference(x2=0.015,P>0.05).Conclusion: When using high frequency ultrasound observed connection nerves of SNT, there were obvious image characteristics, and the rate of high frequency ultrasound in displaying connection nerves of SNT was high, it would be a better value to diagnostic SNT.

Objective: To analyze the imaging features of mycoplasma pneumonia in order to improve the early diagnosis and treatment of mycoplasma pneumonia as well as reduce misdiagnosis. Methods:Multislice CT data of 31 mycoplasma pneumonia patients during 2012.4 to 2014.4 in our hospital were retrospectively analyzed. Results:Multislice CT results showed that 22 cases(70.97%) occurred in one side of lung, in which 12 cases (38.71%) located in the left lung(two cases located in the lobi superior(6.45%)and 10 cases located in the lobi inferior(32.26%));10 cases (32.26%) located in the right lung , in which 4 cases located on the lobi superior (12.90%), four cases at the lobi inferior(12.90%), two cases involved both lobi medius pulmonis and lobi inferior(6.45%). 9 cases (29.03%) were double lung involvement. The changes of CT are diverse which included glass-like images, consolidation, air bronchogram, bronchial vascular bundle thickening, pleural effusion and mediastinal lymph nodes enlargement. Conclusion:Patients with mycoplasma pneumonia always have varied radiographic changes and chest multislice CT has a certain sense of early diagnosis.

Leptin,the product of the obese gene,is a protein produced and secreted primarily by adipose tissue.Leptin initiates several biological effects by binding to its receptors.Many studies have shown that leptin can reduce fat accumulation,but its mechanism is still unclear.This article reviewed the effects of leptin on lipogenesis,and especially on proliferation and differentiation of preadipocytes.

Objective To evaluate the potentiation of neuromuscular block produced by rocuronium during isoflurane anesthesia. Methods Sixty ASA Ⅰ -Ⅱ patients (39 male, 21 female), aged 18-56 yr, weighing 42-88 kg undergoing ENT, oral or facial plastic surgery were enrolled in this study. The patients were premedicated with atropine 0.01 mg?kg-1 . Anesthesia was induced with diazepam 0.1 mg?kg-1 , pethidine 1mg?kg-1 and droperidol 50?g?kg-1. Oro- or naso-tracheal intubation was accomplished under topical anesthesia. After intubation the patients received hydroxydione 2.5 g and fentanyl 5 ?g?kg-1 . The patients were mechanically ventilated. Anesthesia was maintained with inhalation of 50% N2O in O2 alone (groupⅠ) or combined with 0.6% isoflurane (group Ⅱ ) or 1.2% isoflurane (group Ⅲ ) . A bolus of rocuronium 0.6 mg?kg-1 was given during maintenance of anesthesia. Neuromuscular function was monitored using TOF response measured by accelerography (Biometer, Denmark). (1) Onset time (time from the end of injection to maximum depression of T1 ) , (2) the duration of T1 = 0, (3) time from the end of injection to recovery of T1 to 25% , 50% and 90% control, (4) recovery index (time from T, 25%-75%) were recorded. End-tidal isoflurane concentration was monitored.Results The onset time was significantly longer in group Ⅰ[ (1.7?0.2) min] than that in group Ⅱ[ (0.9?0.1) min] and groupⅢ[(0.8?0.1) min] ( P