The present paper summarizes recent studies of chemical compositions and pharmacological activities of Asclepiadaceae plants.

BACKGROUND:Some patients appear to have loose teeth and alveolar abnormalities during orthodontic treatment, and to some extent, the probability of periodontal disease has increased. OBJECTIVE: To analyze the effects of orthodontic tooth movement on alveolar bone morphology and bone mineral density. METHODS:Sixty-two patients undergoing orthodontic treatment were enroled, including 34 males and 28 female, aged from 16 to 30 years. After 6 months of orthodontic treatment, cone-beam CT scanning was used to detect the bone mineral density in anterior tooth, posterior tooth and incisor alveolar regions of the maxila and mandible as wel as the alveolar space between bilateral central incisors and lateral incisors. RESULTS AND CONCLUSION:After 6 months of orthodontic treatment, al the patients had strong teeth with no abnormal mobility. The gums were healthy and pink, and the texture was tough and inactive, with no bleeding during probing. There was no difference in the bone mineral density of maxilary alveolar bone with different alveolar spaces before and 6 months after orthodontic treatment (P > 0.05). However, the bone mineral density in the same alveolar space was reduced significantly after 6 months of treatment (P < 0.05). The bone mineral density of the maxilary and mandibular alveolar bone was varied consistently. In addition, the bone mineral density of the maxilary alveolar bone was significantly higher than that of the mandibular alveolar bone both before and after orthodontic treatment (P < 0.05). These findings indicate that under orthodontic treatment, the bone mineral density of the maxilary alveolar bone may be higher than that of the mandibular alveolar bone, but there is no effect on orthodontic results as wel as the morphology and health of alveolar bone.

BACKGROUND:Silent information regulator factor-1 is an energy metabolism regulator newly received attention in sports science, which playing roles in skeletal exercise-induced muscle mitochondrial biogenesis with other regulatory factors.
OBJECTIVE: To review the effect and mechanism of silent information regulator factor-1 on skeletal muscle mitochondrial biogenesis in exercise.
METHODS:The PubMed database and Highwire database were retrieved with computer for the articles on
exercise, silent information regulator factor-1 and skeletal muscle mitochondrial biogenesis from January 2000 to January 2013 with the key words of“SIRT1, AMPK, PGC-1α, mitochondrial biogenesis, skeletal muscle, exercise”in English. After primary search, the articles about the association between silent information regulator factor-1 and skeletal muscle mitochondrial biogenesis in exercise were selected. Articles on repeated experiment were excluded.
RESULTS AND CONCLUSION:Total y 165 relevant articles were selected, and articles on repetitive research were excluded, so finaly 62 articles were included. As a NAD+-depended deacetylase, silent information regulator factor-1 induced skeletal muscle mitochondrial biogenesis by up-regulated peroxisome proliferator-activated
receptor coactivator after activated during exercise. The molecular mechanism involved adenosine
monophosphate-activated protein kinase and hypoxia-inducible factor 2α. In recent years, the effect of silent information regulator factor-1 on skeletal muscle mitochondrial biogenesis was doubt, the researchers though that silent information regulator factor-1 was not required for exercise-induced muscle mitochondrial biogenesis.
Silent information regulator factor-1 plays an important role in exercise-induced muscle mitochondrial biogenesis. But protein and activity detection methods are different in experimental results.

Activating transcription factor 3 (ATF3) is a protein produced by the cells in the ischemic reperfusion injury,neuronal or liver damage,skin damage,viral oncogene expression or DNA damage.Recent studies have found that ATF3 plays an important role in the occurrence and development of human malignant tumors.

Exfoliated cells in abdominal cavity were studied in 142 patients with gastric cancer. The cells were mainly obtained by irrigation with normal saline during operation. In this series, 53.5%(76/142) of the patients have malignant exfoliative cells. The relationships between the localization and the pathological types of cancer and the positive rate of the exfoliative cells in the abdominal cavity were discussed. No significant differences were found with regard to the TNM staging of the gastric cancer, the positive rate of stage I was 4/5, while those of the stage II,III,IV were 36%, 42.6% and 66.2%, respectively. The five-year follow-up study showed that in the positive exfoliative group, none lived more than 3 years, including 4 cases of TNM stage I, 9 cases of stage II and 63 cases with advanced cancer. However, in the 66 cases with negative cytology, 1 case of stage I patient was alived more than 7 years after operation, and in the 26 cases of stage II, 10 survived more than 5 years. This method is recommanded to detect the early metastasis of gastric cancer in the abdominal cavity. It may be useful in planning a reasonable management for the patient and may give a better understanding about the prognosis.

BACKGROUND: Studies have confirmed that umbilical cord blood stem cells can improve the neurologicalfunction after stroke, but the mechanism of umbilical cord blood stem cells in the treatment of stroke is unclear.OBJECTIVE: To review the effect and mechanism of umbilical cord blood stem cells in the treatment of stroke.METHODS: The first author searched PubMed and CNKI databases for relevant articles published from January2005 to December 2015 using key words of umbilical cord blood stem cells, stroke in English and Chinese,respectively. RESULTS AND CONCLUSION: With the further research on the neural differentiation of umbilical cord blood stem cells,umbilical cord blood stem cells have become a hot research topic in the field of neural system disease therapy. Atpresent, some experimental studies on cell transplantation for stroke have achieved ideal curative effects. However, themechanism of umbilical cord blood stem cells in the treatment of stroke is not clear. Known therapeutic mechanismsinclude the recombination of blood vessels, nerve loops and other damage tissue structures, secretion of variousnutritional factors to reduce endogenous apoptosis, promotion of endogenous blood vessels and nerve regeneration, etc.But the following problems need to be further studied, including whether the transplanted cells can be fully filled toreplace necrotic nerve cells and promote recovery of nerve function; how these survived stem cells establish the neuralconnections; how these transplanted cells reduce the apoptosis and necrosis of the host cells to restore the cell function.

Objective To evaluate the clinical value of dual-energy subtraction digital radiography in diagnosing the pulmonary nodulesand calification in nodules.Methods Using American GE Revolutln DR-XR systems,All 80 patients were examined by dual-energy.Tissus images、bone images and the original digital radiographic images were obtained.Dual-energy digital subtraction images were compared with the original digital radiographic images.Results Of 186 pulmonary nodules proved by CT,126(94 uncalified and 32 calified)were detected by tissues images and bone images and 109(83 uncalified and 26 calified)were detected by the original digital radiographic images.Conclusion Dual-energy subtraction imaging is superior to the original digital radiographic images in detection of pulmonary nodules and calification in a nodule.Dual-energy subtraction images can act as a complement for the original digital radiographic images.

The outcome of patients with malignant middle cerebral artery infarction (mMCAI) is very poor.The mortality of conservative medical treatment is as high as 80％.Several randomized controlled trials published in recent years have shown that hemicraniectomy can significantly reduce the mortality of mMCAI.It is a life-saving therapeutic measure for patients,even if so in patients older than 60 years of age.

Objective To develop the adolescent online game experience scale(AOGES) and tests its reliability and validity,so as to explore the component construct of the adolescents' online game experience.Methods Interviews and open questionnaire investigation as well as the existing scales were used to form the earlier version of the measurement,and the formation of test version were based on the expert group discussion,A total of 785 middle school students completed the AOGES,factor analysis method were used to form the final questionnaire.60 students participated in the retest 3 weeks later.The Online Game Addiction Scale and APIUS were used as criterions.Results The exploratory factor analysis indicated that the AOGES was composed of 40 items and six-factors; The range of factor loading was between 0.40-0.89,and explained 60.42％ of the total variance.The cortfirmatory factor analyses indicated that the six-factor model had good fitting indices (x2/df =2.83,GFI =0.93,TLI =0.92,IFI =0.95,CFI=0.95,RMSEA=0.069).The scores of the AOGES were positively correlated with the scores of the Online Game Addiction Scale and APIUS (r=0.12-0.60,P＜0.05).The Cronbach oα of the AOGES was 0.96 and six subscales were between 0.76-0.95.The test-retest reliabilities of the AOGES was 0.81 and six subscales were between 0.51-0.86.Conclusion It suggests that the Adolescent Online Game Experience Scale may be a sixdimension model which has good reliability and validity.The questionnaire may be suitable for measuring the adolescents' online game experience in China.

Objective To investigate the clinical importance and the relationship between the levels of plasma total homocysteine (Hcy), serum folic acid and vitamin B 12 , and the occurrence and development of ischemic leukoaraeosis (LA). Methods Ischemic LA group and normal control group with 100 cases for each were studied. The 100 cases in ischemic LA group were firmly diagnosed by CT or / and MRI. The levels of plasma total Hcy, serum folic acid and vitamin B 12 of the patients in ischemic LA group were measured, and compared with that in control group. Results The level of plasma Hcy in ischemic LA group (19.79?5.25?mol/L) was significantly higher than that in control group (12.02?2.11?mol/L), while the levels of serum folic acid and vitamin B 12 in ischemic LA group (2.08?0.84ng/ml and 140.13?45.49pg/ml) were lower than that in control group (4.39?1.30ng/ml, 297.45?120.23pg/ml). The differences between two groups were statistically significant (P