Middle cerebral artery occlusion(MCAO) can be used to establish the standard model of focal cerebral ischemia.The method for establishing focal cerebral ischemia model has been improved continuously,from simple behavior score,measurement of infarcted brain area,and morphological observation in the past to functional study,neurobiochemistry,molecular biology,etc.The research of MCAO provides a basis for further study of the pathological characteristics,mechanism and prevention of human cerebral ischemia.This article reviews the recent advancement in the study of MCAO model and cerebral(ischemia.)

Objective: To prepare a new multiple emulsion type gel of paeonol. Methods: Span 80 and Triethanolamine were used as emulsifiers of the multiple emulsion, sodium carboxymethyl cellulose (CMC Na) and polyvinyl alcohol (PVA 124) were substrates of mixed hydrophilic gel. Results: The multiple emulsion type gelprepared possessed the merits of high concentration of drug, uneasily volatilizating, persistentation and good film forming. Conclusion: The multiple emulsion type gel of paeonel has the sustained release action.

Objective To research a new algorithm to get the relative pose parameters of spacecraft during the final approach phase of spacecraft rendezvous and docking(RVD).Methods The method was based on the shape of five non-coplanar points and the model of the pinhole,and the conception of 3D reconstruction was employed.For the shape of the mark was known,the 3-D shape of the cross-mark was reconstructed based on the single visual system,and the closed-form algorithm for relative pose parameters of spacecraft was given.Results The algorithm had been validated by the mathematical simulation,and the precise results of the relative pose parameters were calculated.Conclusion The simulation results show that the algorithm is rather simple and with relative accuracy.Furthermore it is meets exact the need in real time.

Objective To investigate the prevention and treatment for complications of chronic subdural hematoma using intracranial puncture and drainage. Methods Clinical data of 210 cases of chronic subdural hematoma treated by puncture and drainage were studied retrospectively. Results The cure rate was 97 1% (204/210), the recurrence rate was 7 1% (15/210), the incidence of complications was 14 3% (30/210), and the mortality, 0 95% (2/210). Conclusions Intracranial puncture and drainage for chronic subdural hematoma is safe, simple and effective. Prompt and proper treatment for complications encountered is required to obtain an excellent therapeutic effect.

Objective To report the preliminary clinical results of treatment of severely comminuted radial head fractures by replacement with floating cup radial head prosthesis. Methods In the period from August 2002 to June 2005, replacement with floating cup radial head prosthesis was performed to treat 10 cases of Mason Ⅲ , Ⅳ radial head fractures, of whom 9 were acute fractures and 1 old. Results All the patients were followed up for 6 to 40 months (mean 14.9 months). The mean flexion/extension of the elbow was 133.5? /9.0? , while the mean pronation/supination of the forearm was 78.5? /84.0? . There were 3 cases of mild pain, 1 case of mild valgus instability in the elbow and 3 cases of mild weakness. The Broberg and Morrey elbow scores averaged 93. The result was excellent in 6 cases, good in 3 and fair in 1, the excellent and good rates were 90% . Conclusion The replacement with floating cup prosthesis shows satisfying preliminary clinical outcome in the treatment of radial head fractures unable to be reduced, while its long- term result needs further follow- up

Immunosuppression in mice vas induced by intrapelitoneal injection of cyclophosphamide40mg/kg for 3 days. After administration of a decoction of Panax ginseng (5g/kg ) or Trogop-terus dung (5g/kg ), alone or 1 :1 mixture of the above two drugs at doses of 5g/kg and 10g/kg for 7 days, resulted in an increase of thymus gland weight (P

Objective To observe the effects of Buqi Huoxue Decoction on levels of ?-AP,TGF-? and IGF-Ⅱ in rats of multi-factor AD model and investigate the mechanism of treating AD with Buqi Huoxue Decoction. Method Multi-factor AD Model was established with D-galactose and SCOP by abdominal injection,AlCl3 by perfusion and QA by hippocampus injection. All rats were randomly divided into five groups:A (control group),B (pseudo injuried group),C (model group),D (Buqi Huoxue Decoction group) and E (hydergine group). Group D was treated by Buqi Huoxue Decoction and group E was treated by hydergine. ?-AP,TGF-? and IGF-Ⅱ level in the blood of AD model were detected by radio-immuno-assay after treatment course. Results ?-AP,TGF-? and IGF-Ⅱ level in the blood of group D and E were decreased significantly compared with group C after treatment. Conclusion Buqi Huoxue Decoction could decrease the high level of ?-AP,TGF-? and IGF-II in the blood of multi-factor AD model,so as to achieve the therapeutic effect.

Objective To investigate the expression and clinical significance of serum ferritin in patients with lung cancer. Methods The relationship between preoperative serum ferritin level and corre-sponding clinicopathological data in 174 patients with lung cancer and 85 patients with benign pulmonary diseases was reviewed and analyzed. Results The level of serum ferritin in patients with lung cancer was significantly higher than that in patients with benign pulmonary diseases. For non-small cell lung cancer, patients with large-sized,poorly differentiated squamous carcinoma in advanced stage had higher serum ferritin levels than those with small-sized,highly or moderately differentiated adenocarcinoma in early stage. Conclusion Serum ferritin may play a promoting role in the development and malignant progres-sion of lung cancer.

Objective To evaluate clinical effects of the transurethral ureteroscopic lithotripsy(URL) and minimally invasive percutaneous nephrolithotomy(MPCNL) in the management of upper ureteral stones.Methods Clinical data of 258 patients diagnosed as having upper ureteral calculi from January 2001 to December 2004 in this hospital were retrospectively analyzed.The patients were given either URL(225 patients) or MPCNL(33 patients).Results Of the URL,the stone-free rate on one session was 73.8%(166/225) and the failure rate was 26.2%(59/225).The causes for the failure included stone movement to the renal pelvic in 42 patients(18.7%,42/225),unsuccessful manipulation in 14 patients(6.2%,14/225),and conversions to open surgery because of ureteral perforation in 1 patient and ureteral rupture in 2 patients(1.3%,3/225).Of the MPCNL,the stone-free rate on one session was 100%(33/33).Conclusions The application of URL can be interfered with ureteral stricture and twist that are secondary to incarcerated ureteral stones.High stone-free rate,low incidence of complications,and satisfactory reliability can be expected using MPCNL,especially in patients with impacted ureteral calculi accompanied with secondary affection at the same side.

AIM: To investigate the molecular mechanism by which the SARS-CoV S protein induces chemokine IP-10 in airway epithelial cells.METHODS: cDNA microarrays were used to screen the gene expression profiles of human bronchial epithelial cells(16HBEs) stimulated by SARS-CoV S protein.In addition,RT-PCR,EMSA,and Western blotting were performed to analyze the phosphorylation of JAK/STAT signal pathway.The changes of IRF-1 and IP10 gene expression and the influence by the corresponding inhibitors were analyzed.RESULTS: IRF-1,a key transcription factor of the JAK/STAT signal pathway,was activated in human bronchial epithelial cells after stimulation by the S protein of SARS-CoV.The IP-10 gene expression was detected 2 h following the phosphorylation of STAT1 after 15 min,which was blocked by STAT1or JAK2 inhibitors.Electrophoretic mobility shift assay(EMSA) demonstrated that the nuclear proteins bound to ISRE and GAS but not NF-?B DNA motif.CONCLUSION: The SARS-CoV S protein induces IP-10 gene expression in human bronchial epithelial cells through activation of the JAK/STAT signal pathway,suggesting that the JAK/STAT signal pathway activated by virus plays key roles in virus infection related acute lung injury.