1.Progress in the personalized medicine using pharmacometabonomics.
Qing HUANG ; Jiye A ; Guohua ZHOU
Acta Pharmaceutica Sinica 2014;49(11):1491-7
Pharmacometabonomics, as an emerging branch of system biology, has been increasingly used in personalized medicine and showed broad prospects. By means of metabonomics, the complicated and detailed metabolic profile of the patient is described, thus providing more detailed description of the disease phenotype. With this understanding, response of different individuals to the drugs are predicted or evaluated through inherent genetic information of the individual combined with the environmental factors. As a result, appropriate drugs and dosage are chosen, which greatly promotes the realization of the individualized therapy goals. This article describes the emerging field of pharmacometabonomics, and the research results of personalized medicine based on the pharmacometabonomics in recent years are reviewed in detail.
2.Preparation and identification of monoclonal antibody specific to the epithelial cell adhesion molecule
Wenbo LIU ; Huan HUANG ; Guohua ZHOU
Cancer Research and Clinic 2012;(11):721-724
Objective To prepare and identify the monoclonal antibody (mAb) specific to epithelial cell adhesion molecule (EpCAM) and explore the function of the mAb.Methods The EpCAM antigen expressed by the prokaryotic expression systems was used to immunize the BALB/c mice,and then the splenic cells from the mice were fused with the Sp2/0 cells to produce hybridomas secreting specific mAb.The positive clones were screened by the ELISA.The western blot analysis was used to identify the reactivity of the mAb to the antigen.Then the immunohistochemistry staining was used to detect EpCAM expression in the 3 primary colorectal carcinoma tissues.Results Three mAb specific to EpCAM were obtained by ELISA tests.Western blot results indicated that these three kinds of antibodies could react to the EpCAM antigen,but no response to the GST tag.Immunohistochemical staining results identified that these mAb could give positive signals to the primary colorectal carcinoma tissues from patients.Conclusion Three mAb specific to EpCAM are obtained and identified,which contributes to the diagnosis and therapy of the carcinoma in the future.
3.Expression and clinical significance of serum ferritin in patients with lung cancer
Guohua FAN ; Wenyang JIANG ; Jie HUANG
Journal of Clinical Surgery 2015;(4):303-304
Objective To investigate the expression and clinical significance of serum ferritin in patients with lung cancer. Methods The relationship between preoperative serum ferritin level and corre-sponding clinicopathological data in 174 patients with lung cancer and 85 patients with benign pulmonary diseases was reviewed and analyzed. Results The level of serum ferritin in patients with lung cancer was significantly higher than that in patients with benign pulmonary diseases. For non-small cell lung cancer, patients with large-sized,poorly differentiated squamous carcinoma in advanced stage had higher serum ferritin levels than those with small-sized,highly or moderately differentiated adenocarcinoma in early stage. Conclusion Serum ferritin may play a promoting role in the development and malignant progres-sion of lung cancer.
4.The effect of modified citrus pectin on the expression of galectin-3 in liver metastasis from colon cancer
Haiying LIU ; Zhiliang HUANG ; Guohua YANG
Chinese Journal of General Surgery 2001;0(10):-
0.05).Conclusions The expression of galetin-3 was obviously increased in liver metastasis from colon cancer,and MCP can effectively inhibit the development of liver metastasis of colon cancer.
5.Recent progress in research on human neutrophil peptides
Guohua ZHU ; Ming QI ; Yufeng HUANG
Journal of Medical Postgraduates 2003;0(12):-
Human neutrophils defensins, e.g., human neutrophils peptides (HNP), had been found in polymorphonuclear neutrophil and shown broad antimicrobial activity, cytotoxicity and chemotaxis as an important family of human innate defence components. The researches on defensins is becoming a worldwide major focus of biological and medical attention nowadays. The researches on molecular characteristics, biological and pathophysiological activities of HNP are reviewed.
6.Relation between NEW-TOAST classification and glucose level in patients with acute ischemic stroke
Chengguo ZHANG ; Guohua ZHANG ; Shuyun HUANG
Chinese Journal of Neurology 2011;44(10):681-684
ObjectiveTo investigate the relation between each subtype of cerebral ischemic stroke classified by NEW-TOAST criteria and the levels of blood sugar. MethodsA retrospective study in 624 patients hospitalized with acute cerebral ischemic stroke. All the patients were classified using NEW-TOAST classification standard. Blood glucose in patients with different stroke subtypes was recorded, and analyzed for the incidence of abnormal glucose metabolism in each subtype. The correlation of glucose to blood sugar,blood pressure and blood fat was analyzed using odd ratio (OR) and 95% confidence intervals (CI).Results(1) Among 624 patients, the most common stroke subtype by NEW-TOAST classificationis atherothrombosis (AT), followed by small arterial occlusion (SAO). One hundred and nineteen patients (19. 1% ) had diabetes history; another 40 patients(7.5% ) were newly diagnosed with diabetes in this study; and 71 patients ( 11.4% ) were found to have glycoregulation abnormality. The incidence of abnormal glycometabolism was high in patients with AT ( 40. 4% ) and SAO ( 39. 7% ). ( 2 ) Association analysis between stroke subtypes and blood sugar: x2 =14. 83,P =0. 020, r =0. 152; in SAO patients, OR was 1. 925 (95% CI 1. 392-2. 664) ; in the patients with AT, there was no correlation to blood sugar levels.Association analysis of high blood pressure in stroke subtypes: in AT patients, OR was 2. 874 (95% CI 1. 957-4. 222) ; in SAO, OR was 1. 609 (95% CI 1. 100-1. 235). Association analysis of high LDL-C in each subtype: OR in SAO was 1.419 (95% CI 1.026-1. 962) ;No significant correlation of LDL-C in AT patients, P =0. 929 ; (3) There is significant difference of frequency of abnormal glycometabolism between stroke subtypes: x2 =17. 79 ,P =0. 000; between AT and SAO patients, x2 =0. 024,P =0. 877; between AT or SAO patients to other three subtypes, P < 0. 05. ConclusionsAmong the subtypes of cerebral ischemic stroke by NEW-TOAST classification, AT and SAO are the most common subtypes.All the subtypes have correlation to the high level of blood sugar, and SAO has the highest correlation to blood sugar levels. High blood pressure may affect both large vessels and small vessels, while high LDL-C may mainly affect small vessels.
8.Preliminary study on grading of injury from adverse drug reaction
Hongbin SHENG ; Dengxiao HUANG ; Yiyan LU ; Guohua JIANG ; Qimin HUANG
Journal of Shanghai Jiaotong University(Medical Science) 2009;29(12):1515-1517
Objective To design a quantitative criterion and grading system for injury from adverse drug reaction (ADR) in order to serve the compensation system of injury from ADR. Methods Based on the other grading system of injury cases, the independent scores and serial grades were given to the ADR injury of different organs or different levels through two turns of consultation to 27 experts from Shanghai. Results Injury from ADR was graded from stage 1 to stage 10 according to the damage degree, with 1 stand for death and 10 for slightest injury. Conclusion The grading method of ADR based on the characteristics of injury accords with the condition of China, and can provide reference for making up the compensation system of ADR injury.
9.Species Specificity of Male Human's 29 Y-SNP Loci
Zengjie HUANG ; Huifang CHEN ; Guohua FENG ; Lanjiang LI ; Bingying XU
Journal of Kunming Medical University 2016;37(9):40-44
Objective To explore species specificity of 29 Y-SNP loci and to lay the foundation for forensic application.Methods Human DNA and 8 different kinds of common animals' DNA were amplified separately by PCR.The PCR products were analyzed with PAGE.Results Twenty-three out of 29 Y-SNP loci were only amplified by the male human DNA,which indicated a good species specificity.Three loci amplified from human and some animals showed that the length of the product was different between two species.Another three loci amplified from human and animal showed that the length of the fragment was similar between human and animal.Five loci were amplified by PCR products from both male and female samples.Conclusion Most of 29 Y-SNP loci,amplified in male humans,have good species specificity and can be directly used for personal identification and paternity testing.