ObjectiveTo Research on the best condition of transfecting RNAi plasmid into the mice brain micro vascular endothelial cells to interfere the tissue enzymes and protease through the cathepsin B-RNAi-lentivirus.MethodsThe brain micro vascular endothelial cells ( bEND.3 ) were divided into three groups at random:culture medium completely group (NP group),culture medium with 5 mg/L polybrene group ( P1 group) and culture medium with 8 mg/L polybrene group ( P2 group),each group has five different dimensional MOI (1,5,10,15,20),after transfected,48 h,72 h,96 h,the fluorescence rate of GFP protein were counted by a fluorescence microscope to obtain the transfected rate,the MOI of the best optimization condition were observed;After one week,the effect of the most appropriate of MOI to cytoactive and survival rate calculated through MTT.ResultsA small amount of green fluorescent protein(GFP) in each group were observed after transfected for 48 h,the transfection rate of each group were 1％ -9％ ;In the same MOI groups,there was no significant difference between the 72 h and 96 h ( P ＞ 0.01 ) ; In groups of MOI ( 1,5,15 ),the prevalence transfection rate of each group were lower than 20％ and in MOI(10,20) group,the transfection rate were between 71％ -97％ and 60％ - 84％,and the transfection rate in group 10 was significantly higher than group 20 ( P ＜ 0.01 ) ; P1 group showed significantly higher survival rate than that in P2 group by MTT assay [ ( 81.38 ± 6.69 ) ％ vs (62.03 ± 5.13)％,t =15.795,P ＜ 0.001 ].ConclusionThe most optimal conditions of CathepsinB-RNAi-Lentivirus infection bEND.3 depend on MOI(10),polybrene(5 mg/L) and the transfection time of 72 h.

0.05).Conclusion The curative effect of moxibustion and Shenlingbaizhu pills are definite to AIDS associated diarrhea.The treatment can improve the quality of life of AIDS patients with good safety.

Objective To investigate the water quality of finished water of the centralized water supply system in Shenzhen according to 106 indexes of water quality standards of China.Methods The collection and preservation of water samples was according to the standard examination methods for drinking water-collection and preservation of water samples (GB/T 5750.2-2006).The sanitary quality of the finished water samples collected from 35 centralized water supply systems in cities were determined and evaluated according to the Standards for Drinking Water Quality (GB 5749-2006) in May of 2008.Results The average qualified rate of drinking water was 82.9% (29/35) in Shenzhen.Among 106 indexes,4 indexes (such as turbidity,aluminum,manganese and free chlorine residue) exceeded the standard limits in degrees in some centralized water supply system.Conclusion According to the results of the present paper,it is considered that the water quality of the product water from the centralized water supply system in Shenzhen is good.

To summarize the status of chinese medicine syndrome study in chronic prostatitis, emphatically analyze the key problems. In the national standard and textbook, the names of four syndrome types are not unifi ed, difference of symptoms description still exist, and diagnostic criteria of basic syndrome type needs to be clarified. Analysis of literature showed that syndrome differentiation was commonly based on the experience of physician in clinical practice. In addition to the four syndrome types, occurrence frequency of spleen qi deficiency and liver qi stagnation syndrome types are relatively high. Research method of syndrome should be improved urgently. This paper proposes counter measures: Clarify the commonly basic syndrome types and syndrome differentiation standards. Formulate the quantitative diagnostic criteria of basic syndrome types.conduct the prospectively multicenter clinical survey.

Objective To explore the effect of the diet recovery time on postural hypotension after gynecological laparoscopic operation. Methods A total of 300 patients undergoing gynecological laparoscopy were randomly divided into observation group and control group according to the order of admission,150 cases in each group. Patients in observation group took liquid food since recovery from anesthetic, their appetite and dietary levels restored to preoperative level within 24 h after surgery from liquid diet to regular diet. Patients in control group were given routine nursing care of diet after belly operation. Results The diet recovery time and the anal exhaust time of patients in observation group were (21.2±3.5) h and (15.5±4.7) h, the control group were (46.8±5.4) h and (23.4±5.4) h, there was significant difference between two groups (t=7.543,5.126, P<0.05).The incidence rate of orthostatic hypotension in observation group was 9.3% (14/150), the control group was 33.3% (50/150), there wassignificant difference between two groups (χ2=8.907, P<0.05). Conclusions Patients undergoing gynecological laparoscopy took food as soon as possible, their appetite and dietary levels restored to preoperative level within 24 h after operation. It didn′ t increase abdominal distension, but helped the intestinal function recovery and helped to reduce the incidence of postoperative orthostatic hypotension.

Objective To research the effects of establishing core system key risk index in reducing adverse events. Methods Analyzed the causes of the 147 adverse events in 2014. Including the core system implementation of the reasons for the end of the adverse events caused, the data of fundamental reasons in adverse events and the high risk link that because nurses don′t practice the core system. In 2015, randomly checked the 29 nursing units, including 27 wards and emergency outpatient transfusion room, ICU. Contains the implement rates of the core system in transfusion treatment, day and night shifts, doctors′ advice and patients′ identification. In order to quarterly analysis the index and pertinently improve the results, assessors of quality administration council, head nurse in endemic area and attendant watch keeper are chosen to gather index data. Results After one year of management, the key aspects of the core system execution qualified rate has reached 95%, the check of the implementation of the system, the total pass rate compared with before had increased 6.94%, orders execution system implementation of a qualified rate had increased 9.33%, patient identification system implementation of qualified rate had increased 4.29%, the qualified rate of change of comparison the differences were statistically significant (P<0.05). After the establishment of the core system key risk index management, the adverse events had decreased 11.06%(P < 0.05). Conclusion The establishment of the core system key risk index management can effectively improve the implement rates and reduce the nurse adverse events.

Objective: To evaluate the correlation between severity of symptom and SPL amounts in expressed prostatic secretion(EPS).Methods: A total of 164 men enrolled in the study.Scores of CPSI and Chinese medicine syndrome were used to assess the severity of symptom.SPL acounts in EPS were also measured.The correlation between scores of CPSI,Chinese medicine syndrome and SPL acounts in EPS were analyzed.Results: There was no linear correlation between scores of CPSI,Chinese medicine syndrome and SPL amounts in EPS(P

Background Vascular endothelial growth factor (VEGF) plays a key role in the vascular development and neovascularization.Studies have proved that Cathepsin B is related to the formation of neovascularization,but its mechanism is unclear.Objective This study was to investigate the expression of Cathepsin B and VEGF in the retinal neovascularization induced by hyperoxia and the relationship between them.Methods Forty-four 7-day-old C57BL/6J mice were randomly assigned into 4 groups:normal control group,hyperoxia-induced group,normal control (NC)-green fluorescent protein (GFP)-lentivirus (Lv) group and Cathepsin B-RNA interference (RNAi)-Lv group,with 11 mice 22 eyes for each group.The mice in normal control group were survival in natural environment.The other three groups of 7-day-old mice were put in a sealed box of oxygen volume fraction (75±2)％ for 5 days and then sent back to normal environment.The mice of hyperoxia-induced group did not have any drug intervention,while the 12-day-old mice of NC-GFP-Lv group and Cathepsin B-RNAi-Lv group received intravitreal injection of NC-GFP-Lv 1 μl or Cathepsin B-RNAi-Lv 1 μl.All 17-day-old mice were sacrificed and the retinas were collected.The mRNA expression levels of Cathepsin B and VEGF were performed by real-time PCR;the protein expression levels of Cathepsin B and VEGF were detected by Western blot.Results Fluorescence microscope results showed that the layer and branch of retinal neovascularization were less in the Cathepsin B-RNAi-Lv group than those in the NC-GFP-Lv group and hyperoxia-induced group.The relative expression levels of Cathepsin B and VEGF mRNA in each group were significantly different (F =444.89,P =0.00;F =519.78,P =0.00).The relative expression levels of Cathepsin B and VEGF mRNA in hyperoxia-induced group,NC-GFP-Lv group and Cathepsin B-RNAi-Lv group were higher than those in normal control group,and the relative expression levels of Cathepsin B and VEGF mRNA in Cathepsin B-RNAi-Lv group were lower than those in hyperoxia-induced group and NC-GFP-Lv group,with significant differences between them (all at P ＜ 0.05).The relative expression levels of Cathepsin B and VEGF protein in each group were significantly different (F =54.37,P =0.00;F =79.65,P =0.00).The relative expression levels of Cathepsin B and VEGF protein in hyperoxia-induced group,NC-GFP-Lv group and Cathepsin B-RNAi-Lv group were higher than those in normal control group,and the relative expression levels of Cathepsin B and VEGF protein in Cathepsin B-RNAi-Lv group were lower than those in hyperoxia-induced group and NC-GFP-Lv group,with significant differences between them (all at P＜0.05).Conclusions The expression levels of Cathepsin B and VEGF in retinal neovascularization of hyperoxia-induced group were significantly higher than those in normal control group,Cathepsin B-RNAi-Lv can inhibit the mRNA and protein expression of Cathepsin B and VEGF,the expression of VEGF may be influenced by the expression of Cathepsin B.

OBJECTIVE: Using the CI database application software and SPSS to process cochlear implanted patients' data, in order to know what factors influence the cochlear implant effects. METHODS: Design a cochlear implant questionnaire; get answers from young patients' parents through telephone calls. Input the patients' data to the database with our software, analyze the data using SPSS. RESULTS: 120 cochlear implant recipients were investigated. Those patients are all between 9 and 20 years old. 106 parents answered the inquisition. Some beneficial statistical results like Communion ability and method, comprehension ability, and other factors that influence CI effect were elicited. CONCLUSION: Even for Pre-lingual deaf adolescent, cochlear implantation is still a good choice to acquire better communion ability and to improve the life quality. The statistical analysis results of our follow up investigation indicated that the earlier the speech training intervention, and adopting lip-reading, combining with scientific language training after CI operation, the better CI effect could be acquired. And also the speech communion ability could be improved quickly. Besides, the CI database application software provided great advantages to this study.
Adolescent ; Cochlear Implantation ; Cochlear Implants* ; Comprehension ; Deafness ; Follow-Up Studies ; Humans ; Language Therapy ; Parents ; Quality of Life ; Surveys and Questionnaires ; Telephone ; Young Adult

Objective: To evaluate the safety and anticoagulant efficacy of domestic bivalirudin injection during emergent percutaneous coronary intervention (PCI) in patients with ST-segment elevation myocardial infarction (STEMI).
Methods: A total of 75 STEMI patients were randomly divided into 2 groups according to anticoagulant used in emergent PCI procedure. Bivalirudin group, the patients received intravenous domestic bivalirudin, n=40 and Heparin group, n=35. The activated clotting time (ACT) was tested at pre-PCI, 5 minutes after medication, immediately after PCI, 30 minutes, 1 hour and 2 hours after medication respectively. The activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and ifbrinogen (FIB) level were measured at before medication and 6, 24, 72 hours after medication.
Results: All patients in Bivalirudin group had ACT>225s at 5min after medication as PCI requirement, while 1 patient in Heparin group could not reach the requirement and the extra dose was added. Both groups maintained ACT>225s during PCI procedure. Bivalirudin group had the lower ACT levels than those in Heparin group at 30 min, 1-and 2-hour after the medication, P<0.05. The post-PCI levels of APTT, PT, TT and FIB were similar between 2 groups, all P>0.05. The no-cardiac event surviving rate at 30 days after PCI in Bivalirudin group and in Heparin group were similar P>0.05 and the mild bleeding at 24 hours after PCI in Bivalirudin group was lower (0 vs 11.43)%, P<0.05.
Conclusion: Compared with heparin, domestic bivalirudin may take faster effect, with shorter half-life period for anticoagulation during emergent PCI procedure in STEMI patients.