Objective:To determine the promoting effect of pyrrolidine dithiocarbamate(PDTC),an inhibitor of NF-?B,on the sensitivity of human ovarian cancer cell line SKOV-3 to chemoagent cisplatin and the possible mechanisms.Methods:SKOV-3 cells were treated with various concentrations of cisplatin and PDTC combination for different periods.Then the growth inhibition of SKOV-3 cells were observed by MTT assay,expression of I-?B? and P65 protein by Western blotting assay,and apoptosis of cells by flow cytometry.Results:Separate application of both cisplatin(0.1-10 ?g/ml)and PDTC(10-40 ?mol/L L)significantly inhibited the growth of SKOV-3 cells and caused cell apoptosis(P

Cerebral microbleed is a cerebral small vessel disease.The detection rate is 4.5％ to 84.9％in different populations.Vascular cognitive impairment is the general name of various cerebrovascular diseases and their risk factors caused varying degrees of cognitive impairments.Studies have shown that cerebral microbleed is closely associated with vascular cognitive impairment.This article reviews the relationship between cerebral microbleed and vascular cognitive impairment in different populations,different degrees and different locations.

Objective To investigate the expression of multidrug resistance gene 1 product P-glycoprotein(P-gp) when nuclear factor kappa B is induced or inhibited.Methods Ovarian cancer cells SKOV-3 were treated with Docetaxel or combinded with pyrrolidine dithiocarbamate(PDTC),the inhibitor of NF-?B.Western blot assay、 flow cytometry assay and MTT assay were used to measure P65 protein,P-gp,apoptosis of cancer cells,and the survival rate respectively.Results Docetaxel increased P65 protein and P-gp expression,while combind use of PDTC reversed this function.Both Docetaxel(≥(1 ?g/mL))and PDTC(≥(10 ?mol/L)) significantly inhibited the growth of SKOV-3 cells,and caused apoptosis.The combined use of Docetaxel and PDTC at low concentrations significantly inhibited the growth of cancer cells and caused apoptosis as compared with those treated with Docetaxel only.Conclusion The expression of P-gp may be ralated to the activation of NF-?B;Inhibition of NF-?B can enhance the chemosensitivity of ovarian cancer cells to Docetaxel.

An early and accurate response evaluation is essential for clinicians to decide whether to continue the treatment with current thera-peutic regimen or to make necessary changes.This article briefly introduces the development of response evaluation criteria for tumors,elab-orates on the application of radiological evaluation criteria in local interventional therapy or sorafenib treatment of hepatocellular carcinoma (HCC),compares the four radiological evaluation criteria,i.e.,World Health Organization (WHO)criteria,Response Evaluation Criteria in Solid Tumors (RECIST),European Association for the Study of Liver (EASL)criteria,and the modified RECIST (mRECIST),and thinks that EASL criteria and mRECIST are better than WHO criteria and RECIST and can predict the prognosis of HCC patients at earlier time points.

Objective Probe application value of CHN(the standards of skeletal maturity of hand and wrist for Chinese-CHN method)bone age in judicial identification,and analyze factors and related problems which influence its accuracy.Methods 522 patients participated in judicial identification of living age estimation.Two posteroanterior radiographs for the left wrist were shot with respective exposure value for all patients.And two oblique radiographs were added.Bone ages were estimated according to CHN method.Result Among 522 cases were 489 males and 33 females.Bone ages of 129 males reached to tiptop of CHN-18.4 and average bone age of other male patients is 16.5.Bone ages of 14 females reached to tiptop of CHN-17.3 and average bone age of other female patients is 15.7.Among 522 cases there were 388 patients with their CHN bone ages above 16.0,about 74.3%.The group of data indicates that multiple factors can influence accuracy of bone age estimation.Among them change of radiograph position and angle influenced estimation accuracy most.Conclusion If radiographs of high quality can be acquired,CHN method can be proficiently used,and radiologists can pay attention to the effect on estimation accuracy of epiphysis classification by change of radiograph position and angle,then CHN bone age can mostly meet requirements of living age estimation.

Objective To analyze the epidemiological trend and distribution of stroke mortality in residents of Tianjin, China. Methods The study was based on 102 718 died cases with stroke in Tianjin between 1999 and 2006. The death cause and the stroke typing were coded according to the ICD -9 and ICD -10. Standardized mortality rates were calculated for stroke and its subtypes, adjusted for age and sex according to the world standard population of the Year 2000. The age, sex and geographic distribution of the stroke and its subtype mortality were analyzed. Chi-square tests were used to determine the statistical significance of mortality trends and differences. Results Stroke mortality rate in Tianjin declined from 133.52 / 100 000 / year in 1999 to 102.52 / 100 000/year in 2006. Cerebral infarction accounted for more than 50% of stroke mortality. The stroke mortality rate for males (120.17 / 100 000 to 157.74 /100 000) was higher than that for females (84.87/100 000 to 109.31/100 000). Stroke mortality rates elevated with age (P

Objective To investigate the role of local angiotensin Ⅱ (ATⅡ) and its receptor (ATR) in the pathogenesis of diabetic cardiomyopathy. Methods Wistar rats were divided into two groups: 3 month group included control and streptozotocin (STZ) induced diabetic rats and 6 month group included control, diabetic rats, and Losartan treated diabetic rats (3 mg?kg -1 ?d -1 orally from 3rd to 6th month after diabetes was induced). The cardiac function, ATⅡ and ATⅡ receptor were determined. Results The left ventricular diastolic dysfunction appeared in diabetes in 3rd month 〔-dp/dt max : (687?86 vs 864?94)mm Hg/s, P

0.05). The percents of high to 35 ℃ and of low to 10 ℃ of day average temperatures did not possess significance. There were significant differences among the temperatures in spring, summer and autumn. As for the relative humidity and rainfall amount from 2000 to 2005, those in 2000 were the maximum and the former was significant(P

Objective·To investigate the effects of insulin-like growth factor 1 on prion encoding gene (PRNP) expression and amyloid precursor protein (APP) metabolism of PC12 cells. Methods·After PC12 cells were treated with 20, 40, 80 ng/mL IGF-1 for 24 h, real-time PCR was used to detect the mRNA expression levels of PRNP, and Western blotting was used to detect the protein levels of AKT, phosphorylation of AKT (pAKT), ERK and phosphorylation of AKT (pERK). The level of β-amyloid 42 (Aβ42) in supernatant fluid was detected by ELISA. Results·Compared with the blank control group, the expression of PRNP mRNA in Alzheimer's disease (AD) model group was increased significantly (P<0.01). The expression of PRNP mRNA was significantly increased after cells were treated with 40 and 80 ng/mL IGF-1 for 24 h (P<0.01). There was no significant difference in APP protein expression among AD model group and three IGF-1 treatment groups (P>0.05). Compared with the blank control group, the level of Aβ42 in supernatant fluid of model group was decreased significantly with the increasing of IGF-1 concentration. The expression level of Aβ42 was decreased significantly in 40 and 80 ng/ml IGF-1 treatment group (P<0.05). The expression of AKT/pAKT, ERK/pERK in AD model group was significantly increased along with the increasing of IGF-1 concentration (P<0.05). Conclusion·IGF-1 could regulate the expression of PRNP gene and effect the metabolism of APP, which may be associated with PI3K/AKT, MAPK/ERK1/2 signaling pathway.

Objective To conduct the global assessment of methylated DNA status of CpG islands. Methods The modified AIMS (amplification of inter-methylated sites) technology was adopted, which mainly included 3 groups of isoschizomers (methylation-sensitive and methylation-insensitive restricted enzymes). The comparison analysis of methylome among 3 concentrations (6%, 8%, 10%) of polyacrylamide with 8M urea denaturing sequencing gel and the digestion effect among 3 groups of isoschizomers were carried out. The results of personal molecular imager FX system and autoradiography were also compared. Results Genome-wide detection of methylome of CpG islands might be the technical basis for the subsequent analysis. The resolving power and scope bands in 6% polyacrylamide denaturing sequencing gel were superior to those in gels with the other two concentrations. 3 groups of isoschizomers could meet the need of genome-wide detection of methylome of CpG islands. Personal molecular imager FX system and autoradiography could be used together, and they compensated each other. Conclusions The optimized AIMS technology is a kind of high-throughput method to analyze methylome, which is simple, specific, and easy to handle. The 3 groups of isoschizomers can cover most of CpG islands for genome-wide detection and get the ideal results. Only a small quantity of genome DNA is enough to meet the need of clinical detection.