Eighty elderly patients undergoing elective resection for the prostate were randomly divided into two groups, the levobupivacaine (observe group) and bupivacaine (control group) were used for spinal anesthesia, respectively. The level of sensory block and maintain time were T7±4 and (224 ±28) min in observed group, those in control group were T6±3 and (227 ±30) min, respectively ( both P＞ 0. 05). The mean arterial blood pressure of control group was (71 ±8) -(72 ±8) mm Hg (1 mm Hg = 0. 133 kPa) from 5 to 30 min after injection; meanwhile that of observed group was(75 ± 9) mm Hg (P ＜ 0. 05). The incidences of hypotension and nausea were 10% (4/40) and 0% (0/40) respectively in observe group, and those were 30% (12/40) and 15% (6/40) respectively in control group (both P＜0.05). The results indicate that levobupivacaine and bupivacaine have similar anesthetic effects, but levobupivacaine has fewer side effects and is more suitable for elderly patients.

Objective To prepare B-lymphoblastoid cell lines of HLA novel allele B*5610 in a family for further study and identification . Method Isolate mononuclear cells under aseptic conditions from the peripheral blood. After infection with Epstein-Barr virus, the cells were cultured in 20% FBS, 2?g/ml CsA RPMI 1640. Results Immortalized B-lymphoblastoid cell lines of five B *5610 carriers in a family were achieved, and the new genes were inherited stably. Conclusion Our work is important for storing and breeding the precious material of biomedicine because the B *5610 genes in the immortalized B-lymphoblastoid cell lines were inherited stably.

Purpose:Analysing the imaging performance of solitary plasmacytoma of spine,to raise the awareness and the diagnosis accuracy.Materials and Methods: Five cases of spinal solitary plasmacytoma confirmed by clinical pathology were retrospectively analysed,to sum up the imaging performance characteristics.Results: All lesions were single vertebra involved,3 cases in thoracic and 2 cases in lumbar,3 cases occurred vertebral compression fracture.X-ray and CT showed osteolytic destruction,expansive growth and soft tissue mass,MRI showed diversity of signals,and remarkable enhancement after contrast injection.Conclusions: X-ray presents lower diagnostic sensitivity,but contributes to exclude multiple myeloma.CT and MRI can depict the morphological features and extent of tumor regions definitely,CT shows better than MRI on the thickening trabecula and residual bone in the tumor,MRI can provide a lot of information for plasmacytoma,including the diagnosis,identification and staging,which is considered as the best imaging diagnostic methods of plasmacytoma.

Objective:To study the indications and clinical efficacy of video assisted small incision in treatment of infected pancreatic necrosis.Methods:A retrospective study was conducted on 27 patients with infected pancreatic necrosis treated by video assisted small incision at the Department of Pancreatic and Splenic Surgery, Hunan Provincial People's Hospital, from January 2018 to December 2019. The surgical approach, operation time, intraoperative blood loss, postoperative hospital stay and complications were analysed. Postoperative follow-up was carried out at outpatients’ clinic, and the patient's time to full recovery and long-term complications were studied.Results:The 27 patients successfully underwent the operations. There were 22 males and 5 females, aged (50.6±6.2) years. The treatment results were analyzed according to the different surgical approaches: the retroperitoneal approach group ( n=4); the omental sac approach group (n=14); the intercostal space approach group ( n=2); and the combined approach group ( n=7). The operation time was (85.3±31.6)min. The intraoperative blood loss was 65.0(45.2, 121.4)ml. The postoperative hospital stay was 23.0(12.5, 36.1)days. The incidence of complications (Clavien-Dindo grade Ⅲ and above) was 14.8%(4/27). There were 2 patients in this study who were admitted to the intensive care unit due to postoperative hemorrhage: 1 patient responded well to conservative treatment and the remaining patient required interventional treatment. Another patient because of poor results, underwent debridement by laparotomy 2 weeks after the operation. There was 1 patient who developed grade C pancreatic fistula which was cured by surgical treatment 6 months later. On follow-up, 2 patients developed colonic fistula 2 weeks after surgery and 2 patients gastric fistula 1 week and 3 weeks after surgery. These patients responded to conservative treatment. Conclusion:With proper case selection, video assisted small incision could safely and effectively be used to treat infected pancreatic necrosis.

AIM:To explore the protective effects of riboflavin on the kidney in streptozotocin (STZ)-induced diabetic rats.METHODS:Male Sprague-Dawley rats were randomly divided into 3 groups:normal control group,diabetic model group and riboflavin-treated group.Diabetes was induced by a single injection of STZ (dissolved in 0.01 mol/L citrate buffer,pH 4.5,65 mg/kg,ip) in rats.The biochemical methods were used to measure the contents of urine protein and malondialdehyde in the kidney,and the activities of superoxide dismutase (SOD) and catalase (CAT) in serum and renal tissues.Furthermore,the protein expression of TGF-?1 and plasminogen activator inhibitor-1(PAI-1) in renal cortex was detected by Western blotting.The morphological changes of renal tissue were observed under microscope.RESULTS:Compared to the diabetic model group,riboflavin significantly increased the activities of SOD and CAT (P

12,9.41%(148/1578);RDQ≤12,91.58%(1430/1578).Logistic multiple regression analysis of gastroesophageal reflux correlation factor studied:OR= 2.781.Conclusions The results showed:there were close correlation of high salt diet and GERED.

During spermatogenesis, lysine-rich histones in spermatogenic cells are progressively replaced by arginine-rich protamine. In this study, the nucleoprotein transition in spermatogenic cells of Wistar rat was investigated using aniline blue staining, PTA staininig (both to demonstrate lysine-proteins) and NQS (1, 2-naphthoquinone4-sodium sulfonate) staining (to demonstrate arginine-protein). Under light microscope, the nuclei of spermatogonia and spermatocytes were intensely aniline blue positive and the nuclei of young spermatids moderately positive, while the nuclei of late elongated spermatids and spermatozoa were aniline blue negative. The nuclei of spermatogonia and young spermatids were basicallyy NQS negative and there were a few granules of weak NQS positive in the nuclei of primary spermatocytes, while the nuclei of late elongated spermatids and spermatozoa were NQS positive. Under the electron microscope, the PTA positive chromatin in the nuclei of early spermatids with round nucleus (steps 1 to 8)was fibrillar or granular in appearance. Along with the condensation of nuclei of spermatids(steps 9 to 13), the nuclear stain ability increased. The positive chromatin in the nuclei of spermatids (steps 14, 15) was disappeared progressively, in the direction from cranial to caudal along with the further condensation of nuclei. The nuclei of late spermatids with elongated nucleus (steps 18, 19) and spermatozoa were PTA negative. These observations suggest that the nucleoprotein transition from histones to protamine (S_1) occur during spermiogenesis and that this process could be divided into two consecutive steps, i. e. from histones through an intermediate phase of transition proteins to protamine.

Objective To investigate the ambiguity results distribution of HLA-A,B and DRB1 gene sequence-base typing in Guangxi population and to propose the way to resolve.Methods HLA-A,B and DRB1 genes of 1 000 donors in the Guangxi branch bank of China'bone marrow bank were genotyped by PCR-SBT,and then the ambiguity results distribution of the three loci was analyzed.The typing ambiguities resultswere resolved by high-resolution polymerase chain reaction-sequence-specific primers(PCR-SSP) and group specific sequencing primer(GSSP) methods,respectively.Results Among 1 000 samples,at least 1 locus in HLA-A,B and DRB1 genes in 96.7％ samples appeared the ambiguity results,in which the proportions of HLA-A,B and DRB1 loci appearing ambiguity results were 65.7 ％,58.8 ％ and 77.2 ％ respectively.For the samples of detected ambiguity results,single using the GSSP method could resolve the ambiguity typing results of 87.37％ HLA-A,93.54％ HLA-B and 60.49％ HLA-DRB1,using high-resolution PCR-SSP could resolve the ambiguity typing results of 12.63 ％ HLA-A,4.76 ％ HLA-B and 15.29 ％ HLA-DRB1,and the rest 1.70 ％ HLA-B and 24.22 ％ HLA-DRB1 ambiguity results were resolved by both GSSP and high-resolution PCR-SSPs method.Conclusion GSSP and high-resolution PCR-SSPs methods have high abilities to solve HLA ambiguity results both locate inside and outside the sequencing region,respectively.GSSP and high-resolution PCR-SSPs methods are supplement for each other,which can effectively resolve the problem of ambiguity results in high resolution HLA typing.

Purpose:To investigate fMRI and DTI in the assessment of cortical visual impairment in children with periventricular leukom-alacia ( PVL).Materials and Methods: Twenty-four children with PVL were enrolled.Meanwhile,24 age-matched normal controls were recruited for comparison.fMRI scan was performed using a 3.0T MR scanner.Data analysis was performed by statistical parametric mapping software (SPM2).Activated voxels were identified in both groups,t test was used for statistical analysis.DTI was performed by MedlNRIA software and DTI color maps were created from fractional anisotropy (FA) values and the three vector elements,FA values on diffusion tensor color maps were compared between the patients and the controls.AH the FA values of these WM fibers were analyzed by paired t test.The correlation was calculated between FA values and activated voxels of visual cortex for PVL children using SPSS10.0.Results: In all 24 normal children,the maximum response of blood oxygen level-dependent (BOLD) signal was located in the primary visual cortex ( PVC).However,some of the 24 cases of PVL had deviated activation.The active voxels of patients in visual cortex were less than that of controls.All 24 cases of PVL showed a significant mean FA reduction in ICPL and PTR in comparison to the ipsilateral regions of healthy controls.The significantly positive correlation was shown between FA values and activated voxels of visual cortex for PVL children.Conclusion:fMRI and DTI play an important role in the assessment of cortical visual impairment in children with PVL.

AIM: To study the effect of hydrogen sulfide on wound healing of skin ulcer in diabetic rats . METHODS:Male SD rats were randomly divided into 3 groups, including non-diabetic control (NDC) group, untreated diabetic control ( UDC) group, and treated diabetic administration ( TDA) group.Diabetic rats were induced by intraper-itoneal injection of streptozotocin (STZ).After 1 week, wound healing model was prepared by making a round incision ( 2.0 cm in diameter ) on the dorsal skin in full thickness .The rats from TDA group received 2%sodium bisulfide ointment on the skin ulcer wound , and the animals from UDC and NDC groups received control cream .After 21 d of treatment with sodium bisulfide , blood samples were collected for biochemical analysis , including prothrombin time ( PT) , thrombin time (TT), and fibrinogen (FIB) in plasma, as well as the activity of superoxide dismutase (SOD) and the content of malondi-aldehyde ( MDA) in the serum.White blood cells ( WBC) and lymphocytes were also counted .Granulation tissues from the wound were processed for histological examination and Western blot analysis was used to detect heme oxygenase -1 ( HO-1) and tumor necrosis factor α(TNF-α) expression.RESULTS:Compared with UDC group, sodium bisulfide treatment accelerated wound healing of skin ulcer (P<0.01), and increased the activity of SOD in serum (P<0.01) in the diabet-ic rats.The declined number of WBC and lymphocytes , prolonged PT and TT , and decreased FIB levels in rats treated with sodium bisulfied were also confirmed .Pathological section showed that there were inflammatory cell infiltration , and irregu-lar and loose fibril alignment in the granulation tissue of rats from the UDC group , but there were regular fibril alignment and increased angiogenesis in the granulation tissue of rats from the TDA group (P<0.05).Furthermore, sodium bisulfide treatment raised HO-1 protein expression , and decreased TNF-αprotein expression in the diabetic rats .CONCLUSION:Hydrogen sulfide accelerates the wound healing of skin ulcer in the rats with diabetes .The beneficial effect of H 2 S may be associated with formation of granulation , anti-inflammation , and antioxidation .