Eighty elderly patients undergoing elective resection for the prostate were randomly divided into two groups, the levobupivacaine (observe group) and bupivacaine (control group) were used for spinal anesthesia, respectively. The level of sensory block and maintain time were T7±4 and (224 ±28) min in observed group, those in control group were T6±3 and (227 ±30) min, respectively ( both P＞ 0. 05). The mean arterial blood pressure of control group was (71 ±8) -(72 ±8) mm Hg (1 mm Hg = 0. 133 kPa) from 5 to 30 min after injection; meanwhile that of observed group was(75 ± 9) mm Hg (P ＜ 0. 05). The incidences of hypotension and nausea were 10% (4/40) and 0% (0/40) respectively in observe group, and those were 30% (12/40) and 15% (6/40) respectively in control group (both P＜0.05). The results indicate that levobupivacaine and bupivacaine have similar anesthetic effects, but levobupivacaine has fewer side effects and is more suitable for elderly patients.

Purpose:Analysing the imaging performance of solitary plasmacytoma of spine,to raise the awareness and the diagnosis accuracy.Materials and Methods: Five cases of spinal solitary plasmacytoma confirmed by clinical pathology were retrospectively analysed,to sum up the imaging performance characteristics.Results: All lesions were single vertebra involved,3 cases in thoracic and 2 cases in lumbar,3 cases occurred vertebral compression fracture.X-ray and CT showed osteolytic destruction,expansive growth and soft tissue mass,MRI showed diversity of signals,and remarkable enhancement after contrast injection.Conclusions: X-ray presents lower diagnostic sensitivity,but contributes to exclude multiple myeloma.CT and MRI can depict the morphological features and extent of tumor regions definitely,CT shows better than MRI on the thickening trabecula and residual bone in the tumor,MRI can provide a lot of information for plasmacytoma,including the diagnosis,identification and staging,which is considered as the best imaging diagnostic methods of plasmacytoma.

Objective To prepare B-lymphoblastoid cell lines of HLA novel allele B*5610 in a family for further study and identification . Method Isolate mononuclear cells under aseptic conditions from the peripheral blood. After infection with Epstein-Barr virus, the cells were cultured in 20% FBS, 2?g/ml CsA RPMI 1640. Results Immortalized B-lymphoblastoid cell lines of five B *5610 carriers in a family were achieved, and the new genes were inherited stably. Conclusion Our work is important for storing and breeding the precious material of biomedicine because the B *5610 genes in the immortalized B-lymphoblastoid cell lines were inherited stably.

Objective:To study the indications and clinical efficacy of video assisted small incision in treatment of infected pancreatic necrosis.Methods:A retrospective study was conducted on 27 patients with infected pancreatic necrosis treated by video assisted small incision at the Department of Pancreatic and Splenic Surgery, Hunan Provincial People's Hospital, from January 2018 to December 2019. The surgical approach, operation time, intraoperative blood loss, postoperative hospital stay and complications were analysed. Postoperative follow-up was carried out at outpatients’ clinic, and the patient's time to full recovery and long-term complications were studied.Results:The 27 patients successfully underwent the operations. There were 22 males and 5 females, aged (50.6±6.2) years. The treatment results were analyzed according to the different surgical approaches: the retroperitoneal approach group ( n=4); the omental sac approach group (n=14); the intercostal space approach group ( n=2); and the combined approach group ( n=7). The operation time was (85.3±31.6)min. The intraoperative blood loss was 65.0(45.2, 121.4)ml. The postoperative hospital stay was 23.0(12.5, 36.1)days. The incidence of complications (Clavien-Dindo grade Ⅲ and above) was 14.8%(4/27). There were 2 patients in this study who were admitted to the intensive care unit due to postoperative hemorrhage: 1 patient responded well to conservative treatment and the remaining patient required interventional treatment. Another patient because of poor results, underwent debridement by laparotomy 2 weeks after the operation. There was 1 patient who developed grade C pancreatic fistula which was cured by surgical treatment 6 months later. On follow-up, 2 patients developed colonic fistula 2 weeks after surgery and 2 patients gastric fistula 1 week and 3 weeks after surgery. These patients responded to conservative treatment. Conclusion:With proper case selection, video assisted small incision could safely and effectively be used to treat infected pancreatic necrosis.

A high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for the determination of mizoribine in human serum using thiamphenicol as internal standard (IS). The serum samples of mizoribine were precipitated with acetonitrile and separated by HPLC on a reversed phase C18 column with a mobile phase of 0.1% ammonium acetate water solution-methanol (47:53, v/v). Mizoribine and IS were detected in the multiple reaction monitoring mode with precursor/product ion transitions of m/z 258.2/126.0 and 354.1/185.2, respectively. The calibration curves were linear over the range of 0.02-2 microg mL(-1) for mizoribine. The limit of quantification (LOQ) was 0.02 microg mL(-1) with acceptable precision and accuracy. The validated method was successfully applied for the evaluation of a bioequivalence study on Chinese healthy volunteers. The main pharmacokinetics parameters after oral administration of 100 mg mizoribine test or reference formulation were as follows: Cmax (1.00 +/- 0.21), (1.00 +/- 0.22) microg mL(-1); AUC(0-infinity) (6.72 +/- 1.39), (6.48 +/- 1.44) microg h mL(-1); t1/2 (2.77 +/- 0.26), (2.66 +/- 0.29) h; tmax (2.95 +/- 0.78), (2.84 +/- 0.50) h.

Objective Patient safety is key to quality of care in hospital management, for which drug safety is critical. General hospitals are challenged with how to monitor drug adverse reaction (ADR), and motivate their staff to report ADRs in building a hospital safety culture. Methods The PDCA cycle quality improvement process was called into play, to set the ADR criteria and analyze data of inpatients' ADRs from 2005 to 2009, which was followed by measures proposed for ADR continuous improvement. Results A total of 2850 ADR cases were reported in the past 5 years, averaging 570 cases per year. The ADR incidence rate was 3. 28%, of which 43 were severe ADR cases, or 0. 49% of the total cases, accounting for 1. 51% of all the ADR cases. Year to year, the quantity and quality of ADR reporting have been improving in the hospital, witnessing an initial culture of drug safety. In 2008, the hospital was awarded as an advanced ADR center in Beijing. Conclusion The PDCA cycle is an effective means for ADR management, as it can improve the awareness of the medical staff for proactive ADR reporting. Sustained circular management against ADRs can minimize risks and maximize patient safety.Under-reporting and the corrective measures shall be watched over by the hospital in the future.

Purpose:To investigate fMRI and DTI in the assessment of cortical visual impairment in children with periventricular leukom-alacia ( PVL).Materials and Methods: Twenty-four children with PVL were enrolled.Meanwhile,24 age-matched normal controls were recruited for comparison.fMRI scan was performed using a 3.0T MR scanner.Data analysis was performed by statistical parametric mapping software (SPM2).Activated voxels were identified in both groups,t test was used for statistical analysis.DTI was performed by MedlNRIA software and DTI color maps were created from fractional anisotropy (FA) values and the three vector elements,FA values on diffusion tensor color maps were compared between the patients and the controls.AH the FA values of these WM fibers were analyzed by paired t test.The correlation was calculated between FA values and activated voxels of visual cortex for PVL children using SPSS10.0.Results: In all 24 normal children,the maximum response of blood oxygen level-dependent (BOLD) signal was located in the primary visual cortex ( PVC).However,some of the 24 cases of PVL had deviated activation.The active voxels of patients in visual cortex were less than that of controls.All 24 cases of PVL showed a significant mean FA reduction in ICPL and PTR in comparison to the ipsilateral regions of healthy controls.The significantly positive correlation was shown between FA values and activated voxels of visual cortex for PVL children.Conclusion:fMRI and DTI play an important role in the assessment of cortical visual impairment in children with PVL.

Objective To explore the clinical significance of 3.0T dynamic?contrast enhanced MRI scan in the grading of intracranial glioma. Methods The magnetic resonance examination were performed in 40 cases of patients of brain tumors confirmed by surgery pathology(29 pa?tients with glioma),including conventional MRI and dynamic contrast?enhanced MRI. Using Kinetic Modeling?version 3.0 software on the GE 3.0T magnetic resonance workstation calculation of intracranial tumor parenchyma area corresponding quantitative parameters Ktrans and Ve values. The quantitative parameters between any two classification were compared ,and the difference was statistically analyzed. The characteristics of the different level of intracranial glioma's dynamic enhanced scan parameters were preliminary analyzed. The receiver?operating characteristic curve (ROC)analysis of Ktrans value and Ve value was performed,and the diagnosed threshold,sensitivity and specificity were acquired. Results While applying dynamic?contrast enhanced MRI scan acquired Ktrans and Ve values ,both values of high grade gliomas include gradeⅢandⅣwere significantly higher than that of low grade gliomas include gradeⅠandⅡ(P<0.05);there was no statistically difference of the parameters of Ktrans and Ve values between gradesⅠwithⅡand gradeⅢwithⅣ(P>0.05). To identify low grade gliomas with high grade glioma ,Ktrans and Ve diagnosis threshold was 0.204/min and 0.099 respectively. To distinguish between gradeⅡandⅢglioma,Ktrans and Ve diagnosis threshold was 0.247/min and 0.176 respectively. Conclusion Combining quantitative parameters Ktrans and Ve value that come from 3.0T dynamic con?trast enhanced MRI scan with regular enhancement MRI can distinguish low grade gliomas with high grade gliomas glioma ,as well as distinguish gradeⅡwithⅢglioma;However,it is still difficult to identify low grade gliomaⅠwithⅡ,as well as high grade gliomaⅢwithⅣ. The Ktrans and Ve value plays an important role in discriminate different grade intracranial tumors in a preoperative noninvasive way.

AIM: To study the effect of hydrogen sulfide on wound healing of skin ulcer in diabetic rats . METHODS:Male SD rats were randomly divided into 3 groups, including non-diabetic control (NDC) group, untreated diabetic control ( UDC) group, and treated diabetic administration ( TDA) group.Diabetic rats were induced by intraper-itoneal injection of streptozotocin (STZ).After 1 week, wound healing model was prepared by making a round incision ( 2.0 cm in diameter ) on the dorsal skin in full thickness .The rats from TDA group received 2%sodium bisulfide ointment on the skin ulcer wound , and the animals from UDC and NDC groups received control cream .After 21 d of treatment with sodium bisulfide , blood samples were collected for biochemical analysis , including prothrombin time ( PT) , thrombin time (TT), and fibrinogen (FIB) in plasma, as well as the activity of superoxide dismutase (SOD) and the content of malondi-aldehyde ( MDA) in the serum.White blood cells ( WBC) and lymphocytes were also counted .Granulation tissues from the wound were processed for histological examination and Western blot analysis was used to detect heme oxygenase -1 ( HO-1) and tumor necrosis factor α(TNF-α) expression.RESULTS:Compared with UDC group, sodium bisulfide treatment accelerated wound healing of skin ulcer (P<0.01), and increased the activity of SOD in serum (P<0.01) in the diabet-ic rats.The declined number of WBC and lymphocytes , prolonged PT and TT , and decreased FIB levels in rats treated with sodium bisulfied were also confirmed .Pathological section showed that there were inflammatory cell infiltration , and irregu-lar and loose fibril alignment in the granulation tissue of rats from the UDC group , but there were regular fibril alignment and increased angiogenesis in the granulation tissue of rats from the TDA group (P<0.05).Furthermore, sodium bisulfide treatment raised HO-1 protein expression , and decreased TNF-αprotein expression in the diabetic rats .CONCLUSION:Hydrogen sulfide accelerates the wound healing of skin ulcer in the rats with diabetes .The beneficial effect of H 2 S may be associated with formation of granulation , anti-inflammation , and antioxidation .

During spermatogenesis, lysine-rich histones in spermatogenic cells are progressively replaced by arginine-rich protamine. In this study, the nucleoprotein transition in spermatogenic cells of Wistar rat was investigated using aniline blue staining, PTA staininig (both to demonstrate lysine-proteins) and NQS (1, 2-naphthoquinone4-sodium sulfonate) staining (to demonstrate arginine-protein). Under light microscope, the nuclei of spermatogonia and spermatocytes were intensely aniline blue positive and the nuclei of young spermatids moderately positive, while the nuclei of late elongated spermatids and spermatozoa were aniline blue negative. The nuclei of spermatogonia and young spermatids were basicallyy NQS negative and there were a few granules of weak NQS positive in the nuclei of primary spermatocytes, while the nuclei of late elongated spermatids and spermatozoa were NQS positive. Under the electron microscope, the PTA positive chromatin in the nuclei of early spermatids with round nucleus (steps 1 to 8)was fibrillar or granular in appearance. Along with the condensation of nuclei of spermatids(steps 9 to 13), the nuclear stain ability increased. The positive chromatin in the nuclei of spermatids (steps 14, 15) was disappeared progressively, in the direction from cranial to caudal along with the further condensation of nuclei. The nuclei of late spermatids with elongated nucleus (steps 18, 19) and spermatozoa were PTA negative. These observations suggest that the nucleoprotein transition from histones to protamine (S_1) occur during spermiogenesis and that this process could be divided into two consecutive steps, i. e. from histones through an intermediate phase of transition proteins to protamine.