This paper introduces a new method which can judge the degree of burn scar hypertrophy by analyzing chroma of the burn scar. Its technical schedule is as follows: Firstly, the image of the burn scar is captured by using a digital camera. Then the chroma emendation is performed by using an Artificial Neural Network(ANN). At last, the chroma of burn scar is analyzed and the classification of burn scar hypertrophy is given by using a Support Vector Machine(SVM). Compared with clinical evaluation, the result deduced from this method is proved to be effective.

BACKGROUND: The vibration of high speed handpiece during tooth preparation may have adverse effect on the continuous force of the bonding interfaces among the cement, dentin, and post core. OBJECTIVE: To find the effect of different types of cements on the coronal microleakage of fiber post under different tooth preparation timings, and to provide evidence for the clinical operation. METHODS: The extracted mandibular premolars were divided into 3 groups, and they were cemented by Rely X luting, Panavia F and Paracora 5 mL, respectively. Each group was averagely divided into 3 subgroups: A, B, C groups which were prepared 15 minutes, 45 minutes, and 90 minutes after the cements mixed. All roots were then dyed and transparented. The teeth were observed under stereoscopic microscope and the dyeing scores were also recorded. RESULTS AND CONCLUSION: Rely X luting caused the highest coronal microleakage of fiber post, followed by Panavia F, while Paracore 5 mL, brought about the lowest under the same tooth preparation timing. Different tooth preparation timing caused no significant effect on the coronal microleakage of fiber post when the cement had been completely set.

ObjectiveTo investigate the accuracy of percutaneous iliosacral screw fixation in sacroiliac joint fracture-dislocation with minimally invasive guider with CT-guided.MethodsFrom January 2011 to May 2011,8 patients with sacroiliac joint fracture-dislocation were treated using percutaneous iliosacral screw fixation assist with minimally invasive guider in CT-guided,which included 5 males and 3 females,with the average age of 32 years (ranged from 26 to 56 years).All patients suffered with vertically unstable pelvic fractures.Bone traction was used in femoral condyle for 6 cases which displaced more than 2cm in sacroiliac joints.Patients were prone position on the CT bed.First sacroiliac joint CT-scan was performed,then marked the needle position on affected side buttocks after measure the best position and track of needle that expected on CT computer screen.According to the data of CT-scan,the angle of the devices was adjusted,which could control 3D direction of the minimally invasive guider,then insert the Kirschner wire into sacroiliac joint guided with the front end of the sleeve of the minimally invasive guider,confirmed the track of needle was excellent with CT-scan (3D),then the guider was moved out and put the hollow screw (dia 7.3 mm Synthes) into the sacroiliac joint along the Kirschner wire.ResultsAll 8 patients were successfully insert the Kirschner wire.The operation time was from 10 to 20 min(mean,14 min).All screws were in the position expected before surgery,no cut out of bone with the CT-scan immediately after operation,the shape of sacroiliac joint was restored satisfied and the fixation was stable.No numbness and radiation-like pain appeared among the operation in the lower limb for all patients,no case had vascular and neurological complications postoperative.ConclusionThe minimally invasive guider can improve the accuracy,security and simplicity of the percutaneous iliosacral screw fixation with CT-guided,and also avoid the deviation of needle insertion angle that determined by operator himself.

Objective:To investigate the effect of low molecular weight heparin (LMWH) on the inflammatory response of PC12 cells induced by oxygen glucose deprivation (OGD) and its related mechanism.Methods:The PC12 cells were cultured in vitro were randomly divided into sham(control) group, OGD group, LMWH group and blocking agent group. The latter group was divided into six groups: Eritoran+ OGD group, LMWH+ Eritoran+ OGD group, ST2825+ OGD group, LMWH+ ST2825+ OGD group, pyrrolidinedithiocarbamate (PDTC)+ OGD group and LMWH+ PDTC+ OGD group. OGD cell model was established. Cell counting kit-8 (CCK-8) assay was used to detect cell activity. The expressions of toll-like receptor 4 (TLR4), MyD88 and nuclear factor κB (NF-κB) mRNA and protein were detected by real time polymerase chain reaction (qRT-PCR) and Western blot. The concentration of interleukin (IL)-1β, IL-6, tumor necrosis factor-α(TNF-α) and S100β were determined by enzyme linked immunosorbent assay (ELISA). Results:The cell activity of OGD group was significantly lower than that of control group on the first, second, third day ( P<0.05). Compared with OGD group, the activity of LMWH group was increased on the second, third day ( P<0.05), but lower than that of control group ( P<0.01). The mRNA expression of TLR4, MyD88 and NF-κB was significantly increased in OGD group compared with the control group ( F=144.9, F=710.5, 79.51, P<0.01). Compared with OGD group, the mRNA expression of TLR4, MyD88 and NF-κB were significantly decreased after treatment with LMWH ( P<0.01), and the specific inhibitor of TLR4, MyD88 and NF-κB enhanced the anti-inflammatory effect of LMWH. The protein expression of this pathway was consistent with that of the gene. The concentration of IL-1β, IL-6, TNF-α and S100β in OGD group was significantly higher than control group ( P<0.05). After treatment with LMWH, the concentrations of inflammatory factors and S100β were significantly decreased compared with OGD group ( P<0.01). When hinder TLR4, MyD88 and NF-κB respectively by Eritoran, ST2825 and PDTC, the concentrations of inflammatory factors and S100β were significantly decreased, but it was still higher than control group ( P<0.05). Conclusions:OGD can cause pathological damage of PC12 cells, including high expression level of S100β and aggravation of inflammatory reaction. LMWH can improve cell activity, down-regulate inflammatory reaction degree and protect the cells. Using inhibitors of TLR4/MyD88/NF-κB pathway to inhibit the corresponding target, the up-regulation of inflammatory factors by OGD can be inhibited in varying degrees. These suggested that LMWH may regulate inflammatory reaction of PC12 cells induced by OGD through TLR4/MyD88/NF-κB pathway.

Objective To investigate the influencing factors of unplanned rehospitalization with-in one year after surgery among adult renal transplant recipients.Methods The clinical data of 299 recipients who underwent renal transplant surgery in the Department of Organ Transplantation of the Affiliated Hospital of Guizhou Medical University from January 2020 to December 2022 were retrospec-tively analyzed.The recipients were divided into unplanned rehospitalization group and non-rehospital-ization group based on whether they experienced unplanned rehospitalization within one year after sur-gery.Univariate analysis and binary Logistic regression analysis were performed to explore the influen-cing factors of unplanned rehospitalization within one year after renal transplantation.Results Among the 299 recipients,102 experienced unplanned rehospitalization,with an incidence rate of 34.11％.Univariate analysis revealedstatistically significant differences were noted between the two groups in terms of gender,occupational status,preoperative underlying disease,rejection reactions,nosocomial infections,immunosuppressive medication regimens,serum creatinine,cystatin C,serum phosphor-us,serum potassium,and initial hospitalization duration(P＜0.05).Binary Logistic regression a-nalysis showed that having ≥3 kinds of preoperative underlying disease(OR=2.122,95％CI,1.198 to 3.759)and experiencing rejection reactions(OR=3.162,95％CI,1.217 to 8.218)were independent risk factors for unplanned rehospitalization within one year after renal transplantation(P＜0.05).Conclusion The rate of unplanned rehospitalization within one year after surgery is relatively high among adult renal transplant recipients.Having ≥3 preoperative underlying disease and experiencing rejection reactions are independent risk factors for unplanned rehospitalization with-in one year after surgery.Transplantation healthcare teams can develop corresponding strategies tar-geting these risk factors to reduce the rate of unplanned rehospitalization and alleviate the burden of disease.

Objective To investigate the influencing factors of unplanned rehospitalization with-in one year after surgery among adult renal transplant recipients.Methods The clinical data of 299 recipients who underwent renal transplant surgery in the Department of Organ Transplantation of the Affiliated Hospital of Guizhou Medical University from January 2020 to December 2022 were retrospec-tively analyzed.The recipients were divided into unplanned rehospitalization group and non-rehospital-ization group based on whether they experienced unplanned rehospitalization within one year after sur-gery.Univariate analysis and binary Logistic regression analysis were performed to explore the influen-cing factors of unplanned rehospitalization within one year after renal transplantation.Results Among the 299 recipients,102 experienced unplanned rehospitalization,with an incidence rate of 34.11％.Univariate analysis revealedstatistically significant differences were noted between the two groups in terms of gender,occupational status,preoperative underlying disease,rejection reactions,nosocomial infections,immunosuppressive medication regimens,serum creatinine,cystatin C,serum phosphor-us,serum potassium,and initial hospitalization duration(P＜0.05).Binary Logistic regression a-nalysis showed that having ≥3 kinds of preoperative underlying disease(OR=2.122,95％CI,1.198 to 3.759)and experiencing rejection reactions(OR=3.162,95％CI,1.217 to 8.218)were independent risk factors for unplanned rehospitalization within one year after renal transplantation(P＜0.05).Conclusion The rate of unplanned rehospitalization within one year after surgery is relatively high among adult renal transplant recipients.Having ≥3 preoperative underlying disease and experiencing rejection reactions are independent risk factors for unplanned rehospitalization with-in one year after surgery.Transplantation healthcare teams can develop corresponding strategies tar-geting these risk factors to reduce the rate of unplanned rehospitalization and alleviate the burden of disease.

Mycotoxins are secondary metabolites produced by pathogenic fungi.They often contaminate various crops,and are detrimental to human and animal health.Mycotoxins have a variety of toxic effects,such as neurotoxicity,hepatotoxicity,immunotox-icity,teratogenicity,and carcinogenicity.However,the mechanism of immunotoxicity is still unclear.Dendritic cells(DCs),as the most potent antigen presenting cells,play a vital role in initiating innate and adaptive immune responses.Previous studies have found that mycotoxins can affect the endocytosis of DCs,the ability to stimulate T cell activation,the secretion of cytokines and chemokines.Thus,this review is aim to summarize the effects of mycotoxins on DCs-mediated immune responses,which may provide reference for researches to clarify the immunotoxicity mechanism of mycotoxins.