Objective To explore the clinical significance of serum N-terminal pro-brain natriuretic peptide (NT-proBNP) and plasma D-dimer (D-D) in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and stable chronic obstructive pulmonary disease (COPD).Methods The clinical data of 76 patients with AECOPD (AECOPD group) and 68 patients with stable COPD (stable COPD group) were analyzed retrospectively.Serum NT-proBNP,plasma D-D levels and arterial blood gas analysis were measured and compared.Results The levels of serum NT-proBNP and plasma D-D in AECOPD group were (918 ± 271) ng/L and (0.269 ±0.048) mg/L,in stable COPD group were (264 ± 32) ng/L and (0.223 ± 0.042) mg/L.The levels of serum NT-proBNP and plasma D-D in AECOPD group were significantly higher than those in stable COPD group (P ＜ 0.01).The levels of serum NT-proBNP and plasma D-D were negatively correlated with arterial blood oxygen partial pressure (r =-0.181,P =0.030;r =-0.166,P =0.047),and were positively correlated with arterial blood carbon dioxide partial pressure (r =0.763,P ＜ 0.01;r =0.424,P ＜ 0.01).Conclusion Combined detection of serum NT-proBNP and plasma D-D has important clinical significance in evaluating severity of COPD,and may indicate AECOPD.

This article systematically introduces the basic conception,classification and physiological significance of anterior guidance for anterior prostheses.A customized incisal guidance table was introduced to record and transfer the individual value of the patient to a virtual full-adjustable articulator system.The clinic effects of the individual value and average value were also compared.

BACKGROUND:Pyrroloquinoline quinone is found to accelerate Schwann cel proliferation and growth factor secretion, but there is no report addressing its role in articular cartilage and chondrocytes.
OBJECTIVE: To investigate the role of pyrroloquinoline quinone in chondrocyte proliferation and interleukin-1β-induced chondrocyte apoptosis in the articular cartilage of knee joints and to verify the protective mechanism involved.
METHODS: Chondrocytes were isolated from New Zealand white rabbits (1 month of age), digested under aseptic conditions, and cultured in DMEM/F12 in the presence of 10% fetal bovine serum to alow for proliferation until passage 2. Adherent chondrocytes were cultured in serum-free DMEM/F12 medium with 0, 6.25, 12.5, 25.0, 50.0 and 100.0 μmol/L pyrroloquinoline quinone, separately. Proliferation activity was determined by MTT at 48 hours of pyrroloquinoline quinone administration. Cel cycle was determined by flow cytometry at 30 hours after pyrroloquinoline quinone administration. Apoptosis was determined by flow cytometry folowing 24 hours of pyrroloquinoline quinone pretreatment and 15 hours of interleukin-1β induction.
RESULTS AND CONCLUSION: Pyrroloquinoline quinone enhanced chondrocyte proliferation activity, increased percentage of S phase and G2/M phase in a dose dependent manner and reached the peak when the concentration of pyrroloquinoline quinone was 12.5-25.0 μmol/L (P< 0.05). Pyrroloquinoline quinone also inhibited interleukin-1β-induced chondrocyte apoptosis in early and late stage, and 25.0 μmol/L pyrroloquinoline quinone had the best effects (P < 0.05). These findings suggest pyrroloquinoline quinone can promote chondrocyte division and proliferation, and protect the cels from interleukin-1β-induced apoptosis.

Fast-track recovery emphasize applicating a series of effective measures confirmed in the perioperative care of patients,the optimized combination generates synergy effect to maximize alleviating various physical and mental stress reaction in order to expedite patient recovery.Most successful example is the application of fast track colon surgery program.Its safety and effectiveness have been widely confirmed,but the report on pancreas surgery is limited.FTRP is aiming to improve medical and health work efficiency,reduce hospitalization time and hospitalization expenses,improve life quality of patients.Based on the application of FTRP in pancreatic cancer,the relation of morbidity,re-admission and peri-operative mortality rates and clinical significance are reviewed.

Objective:To develop a perioral force measurement system for the infants with cleft lip and palate.Methods:The peri-oral force measurement system of infant with cleft lip and palate is composed of hardware and software.The sensor is metal cantilever. The measurement ranges are 0 -20 and 0 -1 00 g/cm2 ,and the precision is 0.1 g/cm2 .The system was used in 4 cases of infants with unilateral cleft lip and palate before and after cheiloplasty.The results were analyzed by SPSS 1 9.0 software.Results:Before cheilo-plasty the perioral force of labial frenum area was (1 .79 ±0.94)g/cm2 ,that of angulus oris area of normal side and cleft side was (5. 41 ±1 .01 )g/cm2 and (3.1 2 ±1 .55)g/cm2 (P <0.05);after cheiloplasty:the perioral force of labial frenum area was (1 2.73 ±3. 51 )g/cm2 ,that of angulus oris area of normal side and cleft side was (7.64 ±1 .64)g/cm2 and (7.27 ±1 .89)g/cm2 .Conclusion:The perioral force measurement system can be used to measure the perioral force of the infants with cleft lip and palate.

Objective To study the change of mid-regional pro-adrenomedullin(MR-proADM) level in patients with chronic heart failure and its significance.Methods Randomly selected 330 patients with chronic heart failure,including 120 cases of grade Ⅱ and Ⅲ,90 cases of grade Ⅳ,90 cases were selected.,Within 24 h after the entry of the group and 7 d after symptom relieved,serum N-terminal pro-B-type natriuretic peptide(NT-proBNP),MR-proADM,cardiac ultrasound(left ventricular diameter(LVEDD) and left ventricular function(LVEF)) were measured.Ninety physical examination in the same period as healthy control during the same period.The change of MR-proADM level in patients with chronic heart failure and its significance were analyze.Results Compared with the healthy control group,the level of MR-proADM in patients with chronic heart failure(grade Ⅱ:(641.55±106.34) pmol/l;grade Ⅲ:(684.46±101.33) pmol/l;grade Ⅳ:(737.81±479.37) pmol/l) was significantly higher than that in the control group(610.22±60.84) pmol/l),the difference was statistically significant(F=5.33,P=0.001).At the same time,the level of MR-proADM was increased with the aggravation of heart failure,after drug treatment will decline((608.09±100.81),(617.64±94.32),(642.22±163.53) pmol/L).And MR-proADM levels were significantly positively correlated with NT-proBNP levels and NYHA classification in patients with heart failure(r=0.194,P=0.003;r=0.206,P=0.000).Conclusion MR-proADM has important guiding significance in the diagnosis,classification of patients with chronic heart failure.

ObjectiveTo study the effects of angiogenesis inhibitor SU6668 on the growth and metastasis of colon cancer in vivo. MethodsMetastatic model of human colon cancer was established by orthotopic implantation of human tumor tissue into colon wall of nude mice. Mice were randomly divided into control, 5 Fu, SU6668, and combined treatment group (both 5 Fu and SU6668 i.p.) respectively. After six weeks tumor weight, inhibition rates, intratumoral microvessel density (MVD), apoptotic index (AI) and metastasis were evaluated. ResultsCompared with control, tumor growth was significantly inhibited in mice treated respectively with 5 Fu, SU6668 and 5Fu plus SU 6668 with an inhibition rate of 0%, 42 6%, 80 9% and 87 2% respectively. MVD decreased significantly in treated groups \[(13 8?5 2)?(12 3?4 5), (2 4?1 5) and (0 9?0 5)\]. AI increased significantly in treated groups \[(3 6?2 4)%? (7 1?5 7)%, (11 9?3 9)% and (19 9?8 6)%\]. The incidences of peritoneal and liver metastases was significantly inhibited in 5 Fu, SU6668 and combined treatment group (100%? 45 5%, 16 7% and 0; 75 0%? 36 4%, 16 7% and 0). The growth and metastasis of human colon cancer implanted in nude mice were significantly inhibited in the SU6668 group and combined group than that in control group and 5 Fu group ( P

Objective To establish an orthotopic implantation and metastasis model of human colon cancer in nude mice. Methods Tumor cell line SW1116 of human colon adenocarcinoma was inoculated subcutaneously into nude mice to develop implantation tumor.Histologically intact tumor tissue was then harvested and implanted to the colon wall of nude mice to set up a model similar to human colon cancer.The formation of implanted tumor rate, local tumor growth characteristics,and metastasis rates were examined. Results A 100% lymphatic metastasis rate was obtained in this model. The incidences of local lymphatic metastasis, peritoneal and liver metastases were 100%, 91.7% and 75.0% respectively.Emacication and exhaustion of the nude mice were presented in late stage of the experimentation. The median survival time of the tumor-bearing nude mice was 10 weeks. Conclusions The orthotopic implantation tumor and metastasis model provide useful tools for the study of mechanism of metastasis and its treatment of human colon cancer.

Objective To detect mutation of mitochondrial DNA in a chinese pedgree with maternally inherited aminoglycoside antibiotic-induced deafness. Methods The mutation of mitochondrial DNA from all 18 family members of a chinese pedigree with maternally inherited aminoglycoside antibiotic-induced deafness was detected by PCR and DNA sequencing. Results Nine individuals in this pedigree carried A→G mutation at the 1555th bp of mitochondrial 125 rRNA, and the others did not have this mutation. Conclusion Mitochondrial DNA mutation may be one of major factors resulted in aminoglycoside antibiotic-induced deafness in this pedigree.

Objective To investigate the related factors of central nervous system infection in patients who received invasive intracranial pressure monitoring.Methods The clinical data of 63 patients who received invasive intracranial pressure monitoring were retrospectively analyzed.Possible related factors including gender,age,disease,Glasgow coma scale score,emergency operation or selective operation,duration of the operation,drainage tube placement site,drainage tube lifetime,sensor insertion site,sensor indwelling duration,electrolytes,glucose and other complications were studied and compared retrospectively between patients with and without central nervous system infection.All data were analyzed with univariate analysis and multivariate Logistic regression analysis to identify the related factors and independent risk factors associated with central nervous system infection.Results In univariate analysis,drainage tube placement site (x2 =12.345,P =0.002),drainage tube lifetime ((2.5 ± 0.9) d and (6.3 ± 5.7) d in patients without and with central nervous system infection,respectively,t =-4.434,P =0.000),sensor insertion site (x2 =4.701,P =0.030),sensor indwelling duration ((6.5 ± 3.0) d and (8.9 ± 4.6) d in patients without and with central nervous system infection,respectively,t =-2.420,P =0.019) and other complications (x2 =13.086,P =0.000) were the relevant factors of central nervous system infection (P ＜0.05).In multivariate Logistic regression analysis,the sensor insertion site (OR =0.047,P =0.000) and other complications (OR =10.953,P =0.019) were the independent risk factors for the central nervous system infection.Conclusions There are a variety of factors related to central nervous system infection in patients with placement of intracranial pressure monitoring.The sensor insertion site and other complications were the independent risk factors for the central nervous system infection.