Objective:To study the influence of occlusal force change on apoptosis of periodontal ligament fibroblast(PDLF) and expression of proliferating nuclear antigen(PCNA) in the fibroblasts,so as to explore the possible mechanism for remodeling of periodontal ligament.Methods:Animal model of occlusal force change was established by extracting the right first,second and third maxillary molars in male SD rats.The rats were sacrificed at 6,12 hours and day 1,2,3,5,7,14,and 28 d after extracting the teeth(n=6),and their alveolar bone tissues were harvested.H-E staining was used to observe the morphologic changes of the alveolar bone tissues.TUNEL method and immunohistochemistry were used to detect apoptosis and PCNA expression in PDLF.The rats with normal occlusal forces were used as control(n=6).The results were assigned a mean score based on the percentage and the intensity of cells positively stained for TUNEL and PCNA.Results:Animal model of occlusal force change was successfully established.H-E staining showed that the control group had more pycnotic periodontal ligament,orderly fibres,and flatter alveolar bone than the model group.TUNEL result showed that the cell apoptosis reached its peak at 12 h after extracting the teeth(252.67?6.62),significantly higher than that of control group(7.17?2.32,P

Objective To investigate the expression of nuclear factor(NF)-?B and intercellular adhesion molecule (ICAM)-1 in rat′s retina injured by ischemia-reperfusion, and the effect of pyrrolidine dithiocarbamate (PDTC) on the expression of NF-?B and ICAM-1. Methods The model of retinal ischemia-reperfusion was set up in 60 SD rats, which were divided into two groups with 30 rats in each: ischemia-reperfusion group and ischemia-reperfussion with injection of PDTC group. The left cephalic artery of each rat was ligated, and the right side was the control. Every group was subdivided into group 1 hour, 6, 12, 24, 48, and 72 hours after ischemia-reperfusion injury, and with 5 rats in each group. mRNA of NF-?B and ICAM-1 mRNA was measured by in situ hybridization (ISH) method in rat′s retina. Every rat underwent electroretinography (ERG) at the corresponding time before executed by neck breaking. Results In ischemia-reperfusion group, expression of NF-?B and ICAM-1 was detected at the 6th hour after ischemia-reperfusion, reached the highest level at the 24th hour, and weakened gradually later. In ischemia-reperfusion with injection of PDTC group, expression of NF-?B and ICAM-1 was detected at the 12th hour after ischemia-reperfusion, and reached the highest level at the 24th hour but lower than that in ischemia-reperfusion group. No expression of NF-?B and ICAM-1 was found in the control group. The relative recovery rate of ERG a and b wave amplitude in ischemia-reperfusion groups was lower than that in ischemia-reperfusion with injection of PDTC group at every stage(P

Objective To research a pure capacitance sensor for acquisition of cardiac phonocardiogram. Methods Under the phantom power supply (48V), the faint vibration signal of the heart was processed into electric signal by larger pre - treatment circuit module, shielded cable and the sensor pre-amplifier of circuit module. Results With some experiments, it can be proved that the sensor won't have affect on the sound field which frequency band under 1 000Hz and can detect the level of sound field like 20dB:30dB and 150dB or higher. Conclusion So with the sensor, the precise signal which can exactly reflect the status of patients' hearts can be detected, and it suits to work in complex condition for its high sensitivity, wide frequency band and low distortion.

Objective: To study the influence of occlusal force changes on apoptosis related gene Bcl-2 and Bax in rat periodontal ligament fibroblasts (PDLF). Furthermore, to explore the possible mechanism in remodeling of periodontal ligaments. Methods: Animal model of occlusal force changes was established by extracting the right first, second and third maxillary molars in male SD rats. Rats were sacrificed at 6,12 hours and 1, 2, 3, 5, 7, 14 and 28 days after teeth extraction(n=6), and their alveolar bone tissues were harvested. HE staining was used to observe the morphologic changes of alveolar bone tissues and immunohistochemistry were used to detect Bcl-2 and Bax expressions in PDLF. The rats of normal occlusal forces were used as control(n=6). The results were assigned a mean score based on the expressions and the intensity of cell positively stained for Bcl-2 and Bax. Results: Animal model of occlusal force changes was successfully established. HE staining showed that the control group had more pycnotic periodontal ligament, orderly aligned fibres, and flatter alveolar bone than model group. Immunohistochemical staining showed that expression of Bax reached its peak at 12 h after extracting the teeth(267.00?7.82), significantly higher than that of control group(25.67?4.97,P

Objective To examine the current situation of smoke-free policies and the support for comprehensive smoke free policies in Beijing, Shenyang, Shanghai, Changsha, Guangzhou, and Yinchuan. Methods Data from Wave 1 of the ITC China Survey (April 2006-August 2008) were analyzed. The ITC China Survey employed a multistage sampling design across 6 cities: Beijing, Shenyang, Shanghai, Changsha, Guangzhou, and Yinchuan. Face to face interviews were conducted with a total of 4 815 smokers and 1 270 non-smokers across the 6 cities to examine their support for smoke free policies. Multivariate logistic regression models were used to identify factors associated with support for comprehensive smoke free policies. Results None of the six cities had implemented comprehensive smoke free policies. Support for comprehensive smoke free policies in workplaces, restaurants and bars was relatively lower compared to hospitals, schools, and public transport vehicles. Support for comprehensive smoke free policies in workplaces, restaurants and bars were associated with knowledge about the adverse health effects of secondhand smoke (for workplaces OR=1.27, 95%CI: 1.08~1.49; for restaurants and bars OR=1.55, 95%CI: 1.17~2.05). Conclusion More comprehensive smoke free policies are needed in the 6 cities. To improve support for comprehensive smoke free policies, public knowledge about the adverse health effects of secondhand smoke needs to be strengthened.

Objective To study the attentional bias to threatening faces images of high and low social anxiety subjects in the threatening and non- threatening conditions.Methods 23 high social anxious subjects (HSAs) and 24 low social anxious subjects (LSAs) were chosen from 500 volunteers according to the score and divided randomly into two groups,one group was under the threatening condition (expecting to give a short speech) and the other under the non- threatening condition,and then a dot-probe task was performed and the attentional bias score (ABS) was recorded.Results Under the threatening condition,the ABS of HSAs and LSAs were (-2.63±1.83)ms and (0.42±1.36)ms,the different was significant (t=-4.641,P=0.000);under the non- threatening condition,the ABS of HSAs and LSAs were (-0.41±1.56)ms and (0.47±0.67)ms,the different was not significant (t=-1.732,P=0.106).The ABS of HSAs under the threatening and non- threatening condition were (-2.63±1.83)ms and (-0.41±1.56)ms,the different was significant (t=-3.122,P=0.005);the ABS of LSAs under the threatening and non- threatening condition were (0.42±1.36)ms and (0.47±0.67)ms,the different was not significant(t=-0.124,P=0.903).The effect of the interaction between social anxiety and the experiment condition on ABS was significant (F=6.881,P=0.012).The separately main effect of social anxiety and the experiment condition on ABS was significant (F=20.429,P=0.000) and (F=6.413,P=0.015) too.Conclusion Social anxious individuals attend to avoid the threatening faces images,especially under the threatening condition.

Objective:To explore influence of negative emotions on prognosis of patients with coronary heart disease (CHD) after percutaneous coronary intervention (PCI) .Methods :Self‐rating anxiety scale (SAS) and self‐rating depression scale (SDS) were used to assess 90 patients undergoing PCI .According to the score results ,patients were divided into pure depression group (n=28 ,occupied 31.11% ) ,pure anxiety group (n=22 ,24.44% ) ,anxiety com‐plicated depression group (n= 25 ,27.78% ) and normal group (n = 15 ) . All patients were followed up for 12 months ,and Logistic regression analysis was used to analyze risk factors for major adverse cardiovascular events (MACE) .Results:Incidence rates of MACE in pure depression group ,pure anxiety group and anxiety complicated depression group were significantly higher than that of normal group (42.86% vs .40.91% vs .64.00% vs .6.67% , P<0.05 or <0.01) ,and that of anxiety complicated depression group was significantly higher than those of pure depression group and pure anxiety group (P<0.05 both) .Logistic regression analysis indicated that anxiety ,depres‐sion ,anxiety complicated depression were risk factors for MACE (OR = 1.49 ,2.16 ,3.28 , P < 0.05 or < 0.01) . Conclusion : Most patients undergoing coronary intervention possesses negative emotions ,which are risk factors for adverse clinical events and affect their prognosis .

Objective To investigate the effect of zinc finger protein A20 expression on lipopolysaccharide(LPS)response in human monocytes so as to discuss its regulation roles and mechanism to inflammation in vivo and its potential value of clinical application.Methods Cell transfection technique was respectively used to make zinc finger protein A20 transgenic cell model and gene silence cell model,both of which were then treated with LPS.Real-time reverse PCR was employed to analyze the expression of zinc finger protein A20 and ELISA to determine the activity of nuclear transcription factor NF-?B and the levels of dependent inflammatory cytokines including tumor necrosis factor(TNF)alpha.Results After LPS treatment,the NF-?B activity and the TNF-alpha level were significantly lower in the cells with overexpressed zinc finger protein A20 than LPS control cells(P

BACKGROUND:Interlocking intramedul ary nail complications contain nail bent or broken, exit, re-fracture at spiketail or nail hole. Thus, our team designs a novel intramedul ary control ed dynamic nail.
OBJECTIVE:To evaluate the rationality and safety of intramedul ary control ed dynamic nail design and strength, and to give rational proposal for its clinical application.
METHODThe three-dimensional finite element models of composite femur, transverse fractures of the femoral shaft were constructed with intramedul ary control ed dynamic nailing. The stress and strain were detected under vertical loads and gait cycle.
RESULTS AND CONCLUSION:The maximum stress of the intact femur under the compression load was at femoral neck and the medial and lateral aspects of the femoral shaft;while the stresses of fractured femur were at the interface between screw and screw hole. In gait cycle, in case of intact bone, large stresses were found in the distal 1/2 of anteriomedialis of femoral shaft;while the stress distribution in fractured femur was similar with the 
former. Intramedul ary control ed dynamic nailing has the ability of generating compression between fragments. These suggest that intramedul ary control ed dynamic nailing is rational and good at design and biomechanical properties.

OBJECTIVE To observe and compare the application result of two kinds of chemical indicator(CI)card contained in high pressure-steam sterilization chemical monitoring testing package for providing correct credible evidence of supplying material in each batch after high pressure-steam sterilization and to avoid resource lost because of fault estimation.METHODS The chemical monitoring testing package was made according Sterilization Criteria published in 2002,with the 3M 1250 and 1243 CI cards and 1292 biology indicator(BI) to observe the results in tested package after sterilization.RESULTS The BI in chemical monitoring testing package was all qualified,the qualified rate of 1250 was 70%,while of 1243 was 100%.CONCLUSIONS If there is no BI for monitoring,1243 CI card should be chosen in high pressure-steam sterilization chemical monitoring testing package for supplying material in each batch after high pressure-steam sterilization which is not influenced by moisture and the contacted material and is easy to read and evaluate,the resource lost caused by fault estimation can be avoided.