Objective To explore the correlation between social support and subjective well-being of Zhuang elder people in rural area.Methods 320 Zhuang nationnality rural old man were randomly selected from the xiangxian town,the Guangxi Zhuang Autonomous Region.Left behind elderly among them (A group) was 160,non left behind elderly (B group) was 80,five elderly (C group) was 80.General access volume,Memorial University of Newfoundland scale of happiness (MUNSH) and social support rating scale (SSRS) were investigated.Results Memorial University of Newfoundland scale of happiness scores of A,B,C groups were (27.90± 10.61,35.11 ±9.92,31.67± 12.07),and the difference was statistically significant (F=11.45,P＜ 0.05).SSRS scores of A,B,C groups were (34.91±8.13,39.53±6.95,30.31±7.62),the difference was statistically significant (F=26.53,P＜0.05).A,B group's happiness score and objective support was positively correlated (r=0.20,0.25 ; P＜0.05),A,B,C group's happiness score is positively correlated with subjective support (r=0.17,0.36,0.31; P＜0.05),happiness score and utilization degree positive correlation in A,C groups (r=0.23,0.39; P＜0.05).Conclusion Zhuang rural elderly subjective well-being is associated with each dimension of social support,and strengthen social support helps to improve the old man's subjective sense of happiness.

OBJECTIVE:To establish a HPLC method for determining the content of fluvastatin sodium in fluvastatin sodium extended-release tablets. METHODS:An Agilent SB-C18 column was used and the mobile phase was CH3CN-0.1% phosphate acid, 50∶50. Flow rate was 1mL?min-1, wavelength was 234nm and the sample size was 20?L. RESULTS:The fluvastatin sodium concentration had good liner relationship from 4.84～96.8?g?mL-1(r=0.999 9), and the average recovery was 100.1%,RSD=0.9%. CONCLUSION:This method was simple, fast, accurate, and precise, could be used to determine content in fluvastatin sodium extended-release tablets.

OBJECTIVE:To study the characteristics of adverse drug reactions(ADR) induced by gatifloxacin.METHODS:A total of 866 ADR cases induced by gatifloxacin collected in Zhejiang ADR Monitoring Center between Oct.30,2003 and Oct.30,2006 were analyzed with Excel.RESULTS:The common ADR induced by gatifloxacin were lesions of skin and appendages,gastro-intestinal system,systemic and nervous system.Most of the cases were slight in symptoms,but gatifloxacin could also induce severe ADR such as dysglycemia etc that resulting in potential risks to vital human organs.CONCLUSION:ADR monitoring should be emphasized when using gatifloxacin so as to decrease or avoid the occurrence of ADR.

Objective To explore the basic medical mechanism of XinBao pill on electrophysiological characteristics of hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4), and illustrate the mechanism of its therapeutical effect on bradycardia.Methods Human HCN4 mRNA was injected into the Xenopus laevis oocytes, after incubated for 2 ~3 days, channel current properties of HCN4 perfused with 40 mg/L XinBao Pill were observed by double electrode voltage clamp technique.Results At-90mV test potential, compared with control group (no XinBao pill), HCN4 channel peak current and tail current in 40 mg/L XinBao pill group had obvious changes, and V1/2 from ( -103.61 ±3.57)mV to ( -106.42 ±5.33)mV in XinBao pill group, from( -81.11 ±4.26)mV to( -86.36 ±7.44)mV in control group.The values of k from (15.15 ±2.23)mV to (17.33 ±3.58) mV in XinBao pill group, from(11.78 ±0.85)mV to(12.39 ±1.51)mV in control group(n=10).At test potential -90 mV, 40 mg/L XinBao pill perfusion fluid decreased the instantaneous current of(0.15 ±0.24)%, the EC50 was (30.8 ±4.8)mg/L (n=8).At test potential-140 mV~-100 mV level, 40 mg/L XinBao pill group increased the channel activation time constant compared with control group[(226.73 ±31.36)ms vs(143.67 ± 21.44)ms;-140 mV,n=10,P<0.05].40 mg/L XinBao pill group increased the channel deactivation time constant compared with control group [(1293.53 ±95.02)ms vs (647.12 ±61.35)ms;-140 mV,n=10,P<0.05].Conclusion The XinBao pill enhances the instantaneous current of HCN4 in a concentration-dependent manner, and extents channel activation and deactivation processes.

ObjectiveTo investigate the psychological loneliness status of the Zhuang empty nester in rural area,and analyze the influencing factors.Methods 320 cases were randomly extracted in certain groups in xiangxian town,Shanglin County of Guangxi Zhuang Autonomous Region,including 160 empty nester( Group A),80 living with families elderly people (Group B ),80 state-fending elderly people (Group C ).The questionnaire and University of California Los Angeles Loneliness Scale(UCLA) were applied for this investigation.ResultsThe top three single-positive rates of the UCLA of the Group A was their hobby different from others (69.54％),lack of sense of intimacy(65.56％ ) and lack of trust(61.59％ ).The UCLA score was significantly different in the Group A(52.44 ±7.29),the Group B(32.16 ±9.01),the Group C(39.65 ± 8.11 ) (F=178.97,P ＜ 0.05).Multiple regression analysis showed a negative correlation between the UCLA score and the number of great-grandson living with the elderly,a positive correlation between the UCLA score and the interval of the children to visit the elderly.ConclusionThe Zhuang empty nester in rural area was very lonely.It is helpful to alleviate the loneliness of the Zhuang empty nester in rural area through establishing a comprehensive pension system,the duties of children to their parents and the family harmony.

OBJECTIVE:To explore the approach and starting point for the management of hospital high-alert medications so as to enhance the level of drug safety control.METHODS:By reviewing pertinent literature,the background,concept and categories of high-alert medications as well as the practice and experiences of carrying out management on high-alert medications in our hospital were introduced.RESULTS & CONCLUSIONS:The management on hospital high-alert medications has effectively lowered the risks of it.In view of the frequent occurring drug safety events,it is necessary to implement the corresponding management on high-alert medications in medical institution.It is advisable to establish a corresponding list of high-alert medications as well as a supervising system in medical institutions to improve drug control safety level and risk prevention ability.

Objective To compare the serum protein profiles between people with successful aged 80 years and over in Bama versus Nanning,to identify the possible causes of the long life expectancy in Bama.Methods We evaluated the serum protein profiles using surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) with 4 different chromatographic surfaces,including the weak cation exchange (CM10),immobilized metal affinity capture (IMAC) 30-Cu,strong anion exchange (Q10) and hydrophobic surface (H50) protein chip arrays in people with successful aged 80 years and over living in Bama versus in Nanning city.SELDI-TOF-MS had a molecular mass detection range of 2000-50,000 D,with a first signal-to noise (S/N) ratio of 5,frequency of peak cluster of over 15 ％,and second S/N ratio of 2 and peak fluctuation of 0.3％ (m/z).Results The total numbers of serum protein (peptide) peaks for people with successful aging in Bama and Nanning were 71 on CM10,46 on IMAC30-Cu,81 on Q10,and 52 on H50.Among subjects from Bama as compared with Nanning (P＜0.05; mean ＞SD for each protein peak),there were 8 differently expressed protein peaks on CM10,of which 2 were upregulated and 6 downregulated; 7 on IMAC-Cu,of which 2 were upregulated and 6 downregulated; 5 on Q10,all of which were upregulated; and 6 on H50,of which 3 were upregulated and 3 downregulated.Conclusions There are marked differences in the serum protein profiles between people with successful aging living in Bama versus in Nanning.Most of the proteins are downregulated among people aged over 80 years in Bama.

Objective To perform the chromosome detection in 1 237 infertile patients for analyzing the karyotypes results and investigating the relationship between infertility and chromosome abnormalities .Methods The peripheral venous blood samples in 1 237 infertile patients in our hospital from March 2010 to December 2014 were collected ,performed the lymphocyte culture ,ob‐tained cells ,fixed under hypotonic condition ,prepared the section and observed them by microscope after G‐banding treatment .Re‐sults Among 1 237 patients ,111 cases abnormal karyotypes were detected with the total abnormal detection rate of 8 .9% ,in which ,57 cases were sex chromosomes abnormality ,54 cases were euchromosomes abnormality .Conclusion Chromosome abnor‐mality is one of the important causes leading to primary infertility .Infertility caused by chromosome abnormalities is irreversible ,so the chromosome cytogenetic examination is especially important in the diagnosis and treatment process of infertile patients .

Objective To compare complications incidence rates of 8-core and 12-core prostate biopsies guided with transrectal ultrasound retrospectively,and to study intervention ways to decrease complications.Methods The data of 260 consecutive patients undergoing first-time transrectal ultrasound-guided biopsy were analyzed.132 patients underwent 8-core biopsy and 128 patients underwent 12-core biopsy.Results In 8-core group,there were 7 cases of infections,15 cases of gross hematuria,7 cases of rectal bleeding and 6 cases of acute urinary retention,and in 12-core group,5 cases of infections,19 cases of gross hematuria,9 cases of rectal bleeding and 7 cases of acute urinary retention in 12-core group.There are no statistical differences of complications incidence rate between two groups (P ＞ 0.05).when prostate volume ＞ 45 ml,detection rate of prostate cancer in 12-core group was significantly higher than that in 8-core group.Conclusions There are no statistical differences of complications incidence rate between 8-and 12-core prostate biopsy.Homologous measure must be taken to reduce complications.12-core prostate biopsy is recommended as prostate volume ＞ 45 ml.

Objective: To explore clinical features of acute myocardial infarction (AMI) caused by left main (LM) coronary artery lesions and to study the effect of percutaneous coronary intervention (PCI) in relevant patients. Methods: A total of 3514 AMI patients received coronary angiography (CAG) in our hospital from 2000-01 to 2015-12 were studied, those including 36 of infarct-related artery (IRA) as LM. There were 28/36 patients received PCI and 8 received CABG. The clinical features and outcomes in 28 LM disease patients were investigated. Results: The patients included 5 female and 23 male at the mean age of (66.5±8.32) years. There were 16 patients with ST-segment elevation myocardial infarction (STEMI) and 12 with NSTEMI; 21 received primary PCI and 7 had elective PCI; there were 16 patients suffered from cardiac shock at admission. The procedural success rate was 82.1% and the in-hospital mortality was 35.7% (10/28). During (66.1±35.2) months follow-up period, 3 patients had re-NSTEMI and 1 of them received PCI again, 3 patients died. No event survival rate was 66.7%. Conclusion: PCI is feasible for treating AMI patients caused by LM lesions, the in-hospital survival rate was 64.3%; while the MACE occurrence rate during long-term follow-up period has been high.