Chronic kidney disease ( CKD) has become a pandemic.Laboratory assay takes the important role in early diagnosis of CKD.We should develop these assays correctly on the basis of full understanding of CKD and guide clinical use for them in laboratory medicine.

Functional dyspepsia( FD)is a common functional gastrointestinal disorder,its pathogenesis has not been fully clarified. Recent studies showed that gastrointestinal mucosal low-grade inflammation had been found in patients with FD,and impaired mucosal integrity,psychological factor and abnormal brain-gut axis may promote the inflammation formation,which may also contribute to the onset and persistence of FD. This article reviewed the advances in study on gastrointestinal mucosal low-grade inflammation in FD.

Objective To investigate the prevalence of dyslipidemia,hyperglycemia,hypertension and metabolic syndrome(MS)and compare the prevalence rate of dysilipidemia obtained in this study with the previous study during 1984--1986.Methods A total of 16 344 adults in Beijing who attended for the medical examinations were recruited in the study.Subjects were asked to complete questionnaires regarding personal health,lifestyle and family health history.The physical examination emphasized measurement of height,weight and blood pressure.Blood samples were collected after an overnight fast and subjected to serum glucose,TC,HDL-C,LDL-C and TG measurements.MS was diagnosed according to Diabetes Society of Chinese Medical Association(CDS)criteria.Results Dyslipidemia occurred in 36.9%(6 034)of subjects.948 hyperglycaemia cases were found.The prevalence rate of high blood glucose was 5.8%. Hypertension occurred in 17.1% of subjects.Obesity/Overweight occurred in 5 252 subjects with the prevalence rate of 32.1%.On the basis of the diagnostic criteria for MS in China.MS occurred in 12.2% of subjects(14.2% for males and 10.0% for females).In both genders,the rate increased along with the increment of age.65.5% participants had at least one of the metabolic disorders.The prevalence of hypercholesterolemia,hypertriglyceridemia,and abnormally low HDL-C were higher than previous analysis during 1984-1986 in all age groups.Conclusions The prevalence of MS in this large occupational population in Beijing was quite high. The prevalence of hypeflipemia had increased considerably over the past 20 years.Balanced nutrition and reasonable consumption should be undertaken to modify these situations.

Objective To observe the clinical efficacy of centro-square needling at Shangxing (GV23) (so called Ghost Hall point) in treating anxiety after cerebral stroke as a major treatment.Method Sixty patients were randomized into a treatment group and a control group. The two groups were both given Flupentixol and Melitracen tablets (Deanxit). In addition, the treatment group received centro-square needling as the major treatment, and the control group received ordinary needling. Hamilton Anxiety Scale (HAMA), Zung's Self-rating Anxiety Scale (SAS), and Barthel Index (BI) of Activities of Daily Living (ADL) were observed before and after the treatment for evaluating the therapeutic efficacy.Result There was a significant difference in comparing the HAMA score between the treatment group and control group after the treatment (P<0.05). There were no significant differences in comparing the total effective rate and recovery rate between the two groups (P>0.05).Conclusion The treatment group shows a significant efficacy in improving the anxiety symptoms; the effect in improving the anxiety symptoms is insignificant in the control group; the recoveryrate and total effective rate are equivalent between the two groups.

Objective To construct human Cystatin C expression vector and make primary protein purification. Methods A 380 bp cDNA fragments encoding Cystatin C were isolated by RT PCR from human placenta tissue and then inserted into pPIC9 vector. Recombinant pPIC9 Cystatin C was transformed into Pichia pastoris strain GS115. Secreted soluble Cystatin C was produced by induction of the AOX1 promoter with methanol. Recombinant Cystatin C was purified by Hitrap SP cation exchange chromatography. Results The expression level of Cystatin C reached 16 mg/L. The stability of Cystatin C is 3 days in expression system. SDS PAGE revealed that Cystatin C was expressed up to 60% of the total soluble protein. After purified by Hitrap SP cation exchange, the recovery rate was 40.5%. Conclusion Cystatin C has a high secretion in Pichia pastoris yeast and could be recognized by polyclonal antibody of native human Cystatin C. After purification,this protein can be used as a standard and antigen to develop immunoassay for human Cystatin C in serum.

The human-based and patient-centered notion is a significant breakthrough of the management conception of modernized hospitals.The service mode of one station style was first adopted in health examination center in the reform of out-patient service flow.Based on the humanized service notion,we can provide health examination service with high quality and efficiency to various patients by taking multifarious measures such as reforming the department locations,optimizing the health examination course and setting convenience-stations.The service mode of one station style was demonstrated to have not only elevated the quality and efficiency of health examination service,but also increased both the patients satisfaction and the profit and reputation of the hospital.

Objective To explore the influencing factors of gastrointestinal bleeding(GB) in secondary prevention patients with cerebral ischemic stroke(CIS).Methods A total of 616 patients were divided into bleeding group and control group according to the status (yes,no) of suffering GB during the 2years follow-up.Single factor analysis and Logistic regression analysis was used to explore the influencing factors of GB in CIS patients.Results The proportion of age≥65,a history of GB,gastric disease,renal insufficiency,sudden onset,NIHSS ≥12 and CIS ≥2 in the bleeding group was significantly higher than that in the control group (P ＜ 0.05) ; The proportion of combined with statins,proton pump inhibitors and gastric mucosal protective agent in the bleeding group was significantly lower than that in the control group (P ＜0.05) ; The Logistic regression analysis showed that age≥65,a history of GB,gastric disease,renal insufficiency,sudden onset,NIHSS≥12 and the times of CIS≥2 were risk factors of GB; however,combined with statins,proton pump inhibitors were protective factors.Conclusions Aging,a history of GB,gastric disease,sudden onset,higher NIHSS score and the times of CIS ≥ 2 were the risk factors of GB,combined with statins and proton pump inhibitors could reduce the risk of GB.

Objective To further research the biological functions of PES1,the ovarian SKOV3 cell line with inducible stable PES1 expression is established by using Tet-on system.Methods PES1 was cloned into pTRE-Tight vector via PCR and its expression was identified. After transfected the regulating plasmid pTet-on,SKOV3 cells were screened with G418 and re-transfected pTRE-Tight-PES1.The positive cell clones were screened out with hygromycin and were induced by doxycycline (Dox) to definite the best induction concentration.Growth velocity of SKOV3 cells stably expressing PES1 induced by Dox was detected with viola crystallina.Results The SKOV3 cells with inducible PES1 expression were screened out after the cells were transfected pTRE-Tight-PES1 constructed.Dox could dose-dependently induce the PES1 expression with the concentration under 2 mg/L,and 2 mg/L of Dox induced the highest PES1 expression.Growth velocity of SKOV3 cells transfected pTRE-Tight has no significant difference between the SKOV3 cells transfected nothing induced with Dox.However,the SKOV3 cells transfected pTRE-Tight-PES1 grew faster than the cells transfected pTRE-Tight or without transfection in the fourth day (P =0.001 ).Conclusion The inducible stable PES1 expression SKOV3 cells are successfully established and could be used to be an effective cell model to research the biological functions of PES1.The expression of PES1 could promote the growth of SKOV3 cells.

According to the widely use of peripheral inserted center catheters , this paper reflects ethical issues form the aspects such as the evaluation before catheter is lack of standards , the access and appraisal system is lax , the continuation of care does not reach and lack of humanistic care aspects .Furthermore , the article points out that the scientific assessment , grasping the indications strictly , strengthening the norms , stricting access system , taking the initiative to follow -up, strengthening health education , the professional ethics , the humanistic care and so on, can ensure the safety of patients and improve nursing service level as a whole .

Objective To establish the quality standards of Zizhu ointment. Methods The TLC was applied to identify Radix arnebiae and borneol of Zizhu ointment, and the content of borneol was determined by gas chromatography. Results The TLC spots were clear, well-separated and easy to identify. The good linear range of borneol reference substance on calibration curve was 0.048 4-1.210 0 μg, and the recovery was 90%-110%, the relative standard deviation was less than 5%. Conclusion The method is simple and feasible, can be used as the quality control method of Zizhu ointment.