The role of nuclear factor kappaB in intestine injury induced by hepatic ischemia reperfusion was investigated. Eighteen male Wistar rats were divided into 3 groups randomly: sham operation group (group A), hepatic ischemia reperfusion group (group B) and hepatic ischemia reperfusion plus pyrrolidine dithiocarbamate (PDTC) group (group C). The rats in group A were only subjected to laparotomy, those in group B underwent partial hepatic ischemia reperfusion (ischemia for 1 h and reperfusion for 2 h) and those in group C underwent the same procedure as that of group B but received PDTC 200 mg/kg i.v. before and after ischemia. After reperfusion, tissues of jejunum and venous blood were obtained for measurement of TNF-alpha, MDA and MPO. The levels of TNF-alpha in jejunum and venous blood, the levels of MPO in jejunum in group B were significantly higher than those in group A and group C (P<0.05). There was no significant different in the levels of MDA between group B and group C. The severity of histological intestinal injury in group B and group C was similar. Hepatic ischemia reperfusion caused intestine injury, NF-kappaB may play an important role in this course and the targeting of upstream components of the inflammatory response, such as NF-kappaB, may have important therapeutic applications.
Intestines/*pathology ; Liver/*blood supply ; Liver/metabolism ; NF-kappa B/*biosynthesis ; Random Allocation ; Rats, Wistar ; Reperfusion Injury/*metabolism

The effects of the cyclin E expression levels on chemotherapeutic sensitivity of breast cancer cell line were explored. After the cyclin E expression was knockdown in MDA-MB-435 by RNA interference, FACS analysis and SA-beta-gal staining were used to evaluate the response sensitivity of breast cancer cells to DNA damage drugs (adriamycin, etc.). Adriamycin could induce G1 arrest in cyclin E knockdown MDA-MB-435 breast cell line and increase the percentage of cell senescence in cyclin E knockdown MDA-MB-435 cells. It was suggested that cyclin E knockdown could increase the chemotherapeutic sensitivity of breast cancer cells to DNA damage drugs.

Chronic kidney disease (CKD) is a general term for heterogeneous disorders affecting kidney structure and function.It can be detected by routine laboratory tests.Acute kidney injury ( AKI),previously referred to as acute renal failure(ARF), represents a persistent problem in clinical medicine.Despite significant improvements in therapeutics,the mortality and morbidity associated with AKI remain high.A major reason for this is the lack of markers for early diagnosis of AKI.In this paper,we discuss recommendations for AKI and CKD laboratory assessment and management,and future challenges.( Chin J Lab Med,2012,35:773-779)

Objective To evaluate the preventive and therapeutic effects of heparin on dextron sulphate sodium (DSS)-induced colitis in mice. Methods The normal mice (n=16) which had been received DSS orally for 7 days were randomized into two groups, the preventive group with heparin subcutaneous administration, and the control group with normal saline subcutaneous administration. The DSS-induced colitis mice (n=16) were randomized into two groups, the treatment group with heparin and the control group with normal saline subcutaneous injection for 7 days. The preventive and therapeutic effects of heparin were assessed by disease activity index (DAI), histological score, TNF-? mRNA expression using hybridization in situ, and Martius scarlet blue (MSB) fibrin staining used to identify microvascular thrombi. Results Microvascular thrombi in the prevention group significantly decreased compared with those in the controls. Microvascular thrombi were positive in 4 of 8 controls, none in the prevention group (P=0.038). Histological score and TNF-? mRNA in the treatment group significantly decreased compared with those in the controls. Histological score of rectum and transverse colon, and expression of TNF-? mRNA in the treatment group and those in the controls were 1.33 and 1.85(P

ObjectiveTo evaluate the changes of hepatic blood perfusion in cirrhotic patients undergoing pericardial devascularization (PCDV).MethodsHepatic artery and portal vein perfusion of 22 pre- and post- PCDV cirrhotic patients were evaluated with rheohepatogram(RHG) and ultrasonography (USG).ResultsCompared with normal control, the hepatic artery, portal vein effective perfusion and total hepatic perfusion decreased on RHG 〔(0.053?0.011) vs. (0.031?0.009),(0.033?0.011) vs. (0.018?0.008),〔(7.7?3.0) vs. (3.5?1.7), all P

Objective To screen for the differentially expressed genes in breast cancer cell line MCF 7 and tamoxifen resistant breast cancer cell line LCC2 by using cDNA microarray Methods The PCR products of 8000 genes were spotted on chemical material coated glass plates in array The DNAs were then fixed on the glass plate by a serial of treatments The total RNAs were isolated from cells cultured in the flash, and then were purified to mRNA by Oligotex Both the mRNAs from different breast cancer cell lines were reversely transcribed to cDNAs with the incorporations of fluorescent dUTP, for preparing the hybridization probes The mixed probes were then hybridized to the cDNA microarray After high stringent washing, the cDNA, microarray was scanned for the fluorescent signals and showed the differences between the cell lines Results Among the 8?000 target genes, there were 1?892 (23 65%) genes expressing differently between MCF 7 and LCC2 cell lines Bioinformational analysis on those genes was performed Conclusion DNA microarray technology is an effective technique in screening for differentially expressed genes between different tissues and cell lines

To isolate and culture the purified monoclonal neural stem cells from the cerebral cortex of new born mice, new-born mice cerebral cortex was isolated and dissociated to single-cell suspension by mechanical trituration. The dissociated single cells were cultured in serum-free medium. After the formation of neurospheres, single-cell clone culture was performed by limiting dilution and the proliferated single-cell clones were harvested for subculture. Immunocytochemistry was used to detect the specific marker of neuroepithelial stem cells (Nestin) of the primary and monoclonal neurospheres. In the differentiated cells we detected the specific antigen of NF-200 and GFAP. Our results showed that the primary neurospheres expressed Nestin antigen positively. By limiting dilution, we cultured the cell lines from single-cell clone and the monoclonal neurospheres expressed Nestin and had capabilities of self-renewal, proliferation and the potentiality of differentiation into neurons and glial cells. It is concluded that monoclonal neural stem cells which have the ability of proliferation and multi-directional differentiation can be isolated and cultured from the cerebral cortex of new-born mice by limiting dilution.

Objective:To investigate the association of human leukocyte antigen (HLA) class Ⅰ (A, B and C), class Ⅱ (DRB1, DQB1 and DPB1) allelic and haplotypic polymorphisms with acute lymphoblastic leukemia (ALL), acute myeloid leukemia(AML) and chronic myeloid leukemia (CML) in Han nationality of southern China.Methods:The peripheral blood samples of 845 leukemia patients (323 cases of ALL, 350 cases of AML and 172 cases of CML) and 745 healthy blood donors from Han nationality of southern China in Shenzhen Blood Center were collected. The HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 genes were genotyped by using polymerase chain reaction-reverse sequence specific oligonucleotide (PCR-rSSO) and polymerase chain reaction-sequence based typing (PCR-SBT) methods to identify the first 4 digits of HLA alleles. The Arlequin 3.5 software was used to analyze HLA haplotypes. The correlations between HLA allelic and haplotypic polymorphisms and three types of leukemia were statistically analyzed at HLA low-resolution level (the first 2 digits of alleles) and high-resolution level (the first 4 digits of alleles), respectively.Results:P-values were adjusted by Bonferroni correction. In ALL group, the frequencies of A*02 (36.22% vs. 28.26%, χ 2 = 13.41, PC < 0.01) and its haplotype A*02-B*46-C*01 (15.35% vs. 10.23%, χ 2 = 10.90, PC = 0.02), DRB1*12 (15.79% vs. 11.10%, χ 2 = 9.02, PC = 0.03), A*02:03 (9.75% vs. 5.32%, χ 2 = 14.25, PC = 0.002) and its haplotype A*02:03-B*38:02-C*07:02 (3.80% vs. 1.51%, χ 2 = 10.41, PC = 0.02) were higher than those in healthy controls, which implied that these factors could confer risk effect in ALL. In AML group, the frequency of A*11-B*15-C*08-DRB1*15-DQB1*06-DPB1*02 (1.34% vs. 0.07%, χ 2 = 12.54, PC = 0.003) was significantly higher than that in healthy controls, suggesting that the risk effect might be conferred by this haplotype. In CML group, the frequencies of A*02 (36.63% vs. 28.26%, χ 2 = 9.33, PC = 0.02) and its haplotype A*02-B*15-C*04 (2.17% vs. 0.29%, χ 2 = 11.74, PC = 0.02), and DRB1*03:01-DQB1*02:01-DPB1*02:01 (1.86% vs. 0.14%, χ 2 = 13.10, PC = 0.01) were higher than those in healthy controls, which implied that these factors could confer risk effect in CML, whereas the frequency of DRB1*13 (1.45% vs. 5.25%, χ 2 = 9.29, PC = 0.03) was lower than that in healthy controls, suggesting that it was a CML antagonistic gene. Conclusion:Leukemia susceptible or antagonistic HLA alleles and haplotypes are found at low-resolution and high-resolution levels of HLA, which might provide reference for investigating the pathogenesis of leukemia and guiding formulation of effective treatment strategies in Han nationality of southern China.

Objective To sum up our experiences for the diagnosis and treatment of visceral pseudoaneurysms. Methods This study included 14 patients admitted from 1990 to 2000, consisting of 6 hepatic, 5 splenic, 3 gastroduodenal aneurysms. Results Thirteen cases experienced a rupture. Five cases received emergent surgery, which was successful in 2, and fatal in one. Transcatheter embolization was used in 11 cases which was successful in all without complications and recurrence after an average follow up of 2 years. Conclusions Digital substraction angiography has diagnostic value for rupture of visceral pseudoaneurysms. Transcatheter embolization is an effective treatment for the majority of patients with visceral pseudoaneurysms.

Objective: This study aimed to analyze the high medical expenses of hospitalized medical insurance patients for the first ten systemic diseases.Methods: We investigated the medical expenses for the first ten systemic diseases among 10 900 hospitalized medical insurance patients of a hospital,calculated the average expenses and total payment for the treatment of each of the systemic diseases that involved 5% of the total patients,computed their percentage in the total expenses,and compared them with those of all the other hospitalized cases.Results: The medical cost of the 5% of the first 10 hospitalized systemic diseases accounted for 21%-29.9% of the total expenses of all the hospitalized patients,4.3-6.0 times that of the average expenses,and 30.8%-54.9% paid by the patients themselves.In addition,the hospital stay of these patients was 1.5-4.7 times that of the average.Conclusion: The high medical expenses involving 5% of the hospitalized patients with the first 10 systemic diseases amounted to a quarter of those paid by all the hospitalized patients.Effective measures have to be taken to keep high hospitalization expenses at a reasonable level.