Objective To evaluate the efficacy of MINE-ESHAP regimen in treatment of refractory and relapsed non-Hodgkin lymphoma (NHL). Methods Twenty-eight patients with refractory and relapsed NHL were treated with MINE-ESHAP regimen. Results The rate of complete remission was 39.3 %(11/28),of partial remission 21.4 %(6/28),of stable disease 17.9 %(5/28),and of progression 17.94 %(5/28). The midsurvival time was 28.5 months. One patient died of infection during marrow restrain period. The main toxicities included gastrointestinal symptoms and myelosuppression. Conclusion MINE-ESHAP regimen is sate and could be employed in treatment of the patients with refractory and relapsed NHL.

AIM: To investigate the protein patterns of bone marrow mesenchymal stem cells(MSCs) induced by 5-azacytidine and Shuanglong Formula(Radix et Rhizoma Ginseng,Radix et Rhizoma Salviae Miltiorrhizae) and molecular mechanism of induced differentiation was discussed at the level of proteome. METHODS: MSCs extracted from Chinese miniswine were induced by 5-azacytidine and Shuanglong Formula.Proteins were separated by two-dimensional gel electrophoresis,and were detected by silver staining method.The image was analyzed by PDQuest software,and the intensities of spots in the gels were used to quantify the expressions of proteins.Proteins of interest were chosen for in-gel digestion and MALDI-TOF-MS analysis,and then further identified by peptide mass fingerprinting(PMF). RESULTS: 16 altered proteins were identified by PMF and their regulation might be caused by the addition of medicate sera containing the active components of Shuanglong Formula.The effects of medicated sera containing Shuanglong Formula on the induced-differentiation could be discussed from the regulated expression of functional proteins; CONCLUSION: Shuanglong Formula may promote the 5-azacytidine-induced differentiation of bone marrow MSCs in vitro,and our study indicate that proteomics can be used for the analysis of molecular mechanism for different cell process.