Objective: To study the spatiotemporal expression pattern of histone acetyltransferase steroid receptor coactivator-1 (SRC1) in developing mouse heart, so as to explore the relationship of SRC1 with heart development. Methods: The normal mouse hearts were collected at E7.5-E18 and 1 day and 3 months after birth; 9 specimens were chosen for each time point to observe the spatial expression of SRC1 by immunohistochemistry technique, and 6 specimens of each time point were used to examine the temporal expression of SRC1 protein by Western blotting technique and to plot the time-dose curve. Results Immunohistochemistry showed no expression of SRC1 in the heart primordium at E7.5; only very weak SRC1 expression was found in the cardiac tube at E8. 5-E9.5. Weak SRC1 expression was found in the trabeculae after E10.5 and relatively strong and widespread expression was found in other heart regions. Western blotting results demonstrated that SRC1 protein expression at E10.5 was significantly lower than those at E11.5 and E12.5 (P<0.05), and was not significantly different with those of other time points after ElO. 5 (P>0.05). SRC1 expression reached the peak at E11. 5-E12.5, and there was no significant difference between the two time points (P>0.05). SRC1 expression gradually decreased after Ell. 5-E12.5 till the adulthood, and there were no significant differences in the expression after E13.5 (P>0.05). Conclusion: Widespread distribution of SRCl is present in the developing mouse heart after ES. 5, and the expression is in a dynamical spatiotemporal pattern, suggesting that SRC1 may take part in the overall regulation of the heart development, and it might has a closer relationship with the early induction of the heart septa.

Adult ; Female ; Health Knowledge, Attitudes, Practice ; Health Services Needs and Demand ; Humans ; Male ; Middle Aged ; Occupational Health ; Steel

Objective To draw up the Chinese Words Reading Test and try to use it as way of premorbid intelligence. Methods 373 health middle-elderly people as study subjects, from five communities in Shanghai city, are between age 55 and 85, and their edueational background is junior high school or above, and their MMSE scores are more than 24 points. 34 of the cases have finished CWRT and Weehsler Adult Intelligence Scale (WAIS) Chinese Revised edition. The main analysis indicators of CWRT: CWRT-Ⅰ for number of right reading 50 characters and CWRT-Ⅱ for 50 words. Results Both CWRT-Ⅰ and CWRT-Ⅱ are relative to education level( r = 0.462,0.489, and P < 0.01), while the correlation with age is not signifieant. Performance of the male participants are better than that of the female. The coefficient of correlation between scores of CWRT-Ⅰ and full IQ of WAIS is 0.79. The mean and standard deviation of scores of CWRT-Ⅰ and CWRT-Ⅱ for the normal elder people with different education levels and gender are provided. Conclusion CWRT is not very diffieuh or easy, and is highly relative to score of WAIS. So it can be used as an evaluating tool for premorbid intelligence.

Objective To study the influence of dietary test designed by Nutritional Department of Henan Provincial People's Hospital on diabetic inpatients' dietary cognition. Methods A total of 134 diabetic inpatients were selected and tested with standard diabetic meaL Self-reporting of oil, salt, staple food, vegetables and meat intake was evaluated. Nonparametric tests were used for statistical analysis. Results There were significant differences in self-reporting of oil,salt,staple food and meat intake between pre and post test (Z= -4.642, -8.700, -2.218, -5. 528,all P<0.05) ; however,no significant change of self reporting vegetable intake was found ( Z = - 1. 385, P > 0. 05 ). Conclusion Dietary test can significantly change patients' cognition about their daily dietary intake.

Objective:To compare the cognitive functions between elderly of Shanghai, Hong Kong and San Diego.Method:104 elderly in Shanghai was evaluated by the Chinese version of Mattis Dementia Rating Scale (DRS)and mini-mental state examination (MMSE), the results were compared with that of 104 persons in Hong Kong and 150 elderly participants in San Diago.Result:Compared with Hong Kong participants, Shanghai participants performed significantly higher on one item, but there were significantly differences in five items between Shanghai participants and the San Diego participants which age and education were matched.Conclusion:Some DRS subscales or individual items may be susceptible to cultural differences.

Objective To evaluate the soft tissue profile in adults with unoperated isolated cleft palate. Methods Twenty patients older than 16 years(median age,18.4) with unoperated isolated cleft palate were enrolled.Soft tissue lateral cephalograms were traced and compared with those of cleft operated patients and class-Ⅰocclusion individuals.The results of various measurements were analyzed with ANOVA by SPSS11.5. Results In adults with unoperated isolated cleft palate,there was restriction at the top of the upper lip,and the antelabrum of upper lip and the lower lip were found normal. Conclusion The soft tissue profile in adults with unoperated isolated cleft palate exhibits almost the same characteristics as the corresponding hard tissue,and there is covering over effect of the soft tissue to some extent.

Environmental Exposure ; adverse effects ; Humans ; Incineration ; Industrial Waste ; Neoplasms ; epidemiology ; Population Surveillance ; Refuse Disposal ; methods ; Risk Factors

China ; epidemiology ; Humans ; Venous Thromboembolism ; diagnosis ; drug therapy ; epidemiology

Objective To investigate the effects of different dose of tranexamic acid in fibrinolysis during liver transplantation. Methods Sixty ASA Ⅱ~ Ⅳ liver transplant recipients, were randomly, double-blind assigned to one of 3 groups (n = 20): group control (group C), group tranexamic acid 1 (group T1) and group tranexamic acid 2 (group T2). The patients in group C received a loading dose of normal saline 10 mL, then continued infuse normal saline at 20 mL/h until neohepatic phase 120 min, while in other two groups, patients received a loading dose of tranexamic acid 1 g, totally 10 mL, followed by continuous infusion at 10 mg/(kg·h) in group T1 or 20 mg/(kg·h) in group T2 until neohepatic phase 120 min. Prothrombin time, fibrinogen, fibrin degradation product and D-dimers were measured before operation (T0), 120 min after the skin incision (T1), nonhepatic phase 30 min (T2), neohepatic phase 120 min (T3). Blood loss, fresh frozen plasma dosage, fibrinogen dosage and thromboembolic events were recorded. Results The plasma concentration of fibrin degradation product and plasma concentration of D-dimers were different in the 3 groups, group T2< group T1 0.05). Conclusions Continuous infusion of tranexamic acid can inhibit fibrinolysis during liver transplantation. No adverse event of thrombosis was detected. Larger dose of tranexamic acid can reduce blood loss and fresh frozen plasma transfusion.

Objective To evaluate the clinical outcomes of a hovel porous β-tricalcium phosphate (β-TCP)and control allograft for the repair of lacunar bone defects caused by solitary bone cyst curettage.Methods From January 2003 to December 2008,the patients with solitary bone cyst were randomized into an experimental(55 cases)and a control(40 cases)group.The control group received particulate allograft bone as the graft material,and the experimental group received β-TCP.At 1 week,1,2,3,6,12,24,48months after surgery,a new radiographic scoring system was employed to calculate the biodegradation of bone graft and evaluate the influence of multiple factors.Histologic characteristic of the degradation process of β-TCP were also evaluated.Results All the cases were followed up for average 28.4 months.Radiographic semi-quantitative analysis revealed that the biodegradation effieiencies were not significantly difierent between β-TCP and allografts(P=0.424).Degradation percentage of the implanted β-TCP or allograft was higher in younger patients than those in the older ones.Degradation of β-TCP was significantly higher than that of allografts over 3 years after surgery(P=0.04).In the experimental group,β-TCP degradation was greater in the loose packing treatment than that in the dense packing treatment (P=0.03).Histological observation demonstrated that the process of new bone formation accompanied the degradation of β-TCP.Conclusion The interporous β-TCP could be an advantageous alternative to allografts for repair bone defects caused by bone cyst.The clinical application of β-TCP is safe and reliable,which shows better biodegradation and osteogenesis than allografts in long-term follow-up.