Objective To probe into the methods of postoperative nursing care for the patient undergoing domino liver transplantation.Methods The key of postoperative care included:monitoring of vital signs,the condition of cruor and the state of drainage rigorously; keeping the balance of intake and output; taking the immunosuppressive drugs correctly; proper diet; prevention of complication;giving psychological nursing.Results The patients passed the perioperative period successfully and were all cured.Conclusions To aim at the characteristic of this operation,implement holistic nursing care,is an important link to assure the patients of domino liver transplantation to recover smoothly after the operation.

Objective: To investigate the effect of GLP1 on the blood glucose in type 2 diabetic rats,and its protective effects on the islet B cells.Methods: Thirty rats were divided into three groups: spontaneous type 2 diabetes animal model OLETF rats,GLP-1 [from the twelfth week 56 ?g/(kg?d),sc] therapy group and LETO rats as control.In the 14th and 20th weeks,standard OGTT including fast and 2 h-plasma glucose were measured respectively.In the 14th weeks,3 rats from each group were killed randomly,and the rest of the rats were killed until the 20th week.Immunostaining with the marker of PCNA,TUNEL,and insulin assessed metabolic changes in the islet. Ultrastructure of the B cell was observed with the electronic microscope.Results: In the 14th and 20th week,AUC for insulin were higher in treated animals(10.86?1.56 vs.9.07?1.28,P

Analysis of metabolic activities of cytochrome P450 isoenzyme is a crucial index to study drug/toxicant metabolism, drug-drug interaction, polymorphism and et al. Due to this practice, it is important to use the proper probe substrate and to conduct the experiment under optimal conditions. The validation information in literatures on the most common and newest in vitro probe substrates have been reviewed.

AIM: To establish the technique of precision-cut fibrotic liver slice (PCLS) and grope the optimal cultural conditions for researching the liver xenobiotic metabolism in vitro and the drug interaction. METHODS: Complex factors (higher fat diet, alcohol and CCl 4) were used to make the animal model of liver fibrosis. Fibrotic liver slices were prepared and cultivation system was established. Lactate dehydrogenase (LDH) leakage, glutathione S-transferase (GST) activity and 3[4,5-Dimethythiazole-2-yl]-2,5- diphenyltetrazolium bromide (MTT) reduction were chosen as indexes to assess the viability of the slice in different thickness, medium pH and cultural time. RESULTS: Rats were in earlier hepatic fibrosis after administration for 3 weeks. When the thickness of slices was 300 ?m and medium pH was 7.0, the LDH leakage, GST activity and MTT reduction could maintain on a steady level in 6 h. CONCLUSION: A 300 ?m of thickness, 7.0 of medium pH and 6 h of cultural time are the optimal slicing and culturing conditions for fibrotic liver slice.

Objective To study the efficacy and adverse reactions of the treatment on different types of infantile hemangiomas (IH) with different doses of propranolol .Methods One hundred and fifty cases of IH without contraindications were randomly treated with different doses of propranolol (low dose group:1 mg?kg -1 ?d-1 ;high dose group:2 mg?kg -1 ?d-1 ) under ECG monitoring inferior half an hour to breastfeeding taking ,and stay in hospital for observation for 6 to 24 hours after the treatment . The patients returned to the hospital for review every month(ECG and blood RT ;lung function in necessary) .Results The curative effect is better on strawberry IH than that on flat IH ,also better on deeply IH than that on superficial IH .The adverse reactions oc‐curred relatively slight in the low dose group and there was no significantly statistical differences on the treatment effects between the both groups(P>0 .05) .Conclusion It is safe and effective to treat IH with propranolol .

Chromosomes, Human, Pair 22 ; Chromosomes, Human, Pair 9 ; Humans ; Leukemia, Promyelocytic, Acute ; genetics ; Male ; Middle Aged ; Translocation, Genetic

Objective:To investigate the role of early enteral nutrition in preventing functional delayed gastric emptying(FDGE)after radical distal subtotal gastrectomy.Methods:A retrospective review was performed in clinical data of patients with gastric cancer undergoing radical distal subtotal gastrectomy in our hospital from March 2004 to March 2008.There were 71 patients receiving early enteral nutrition (group EEN,n=71) after operation and 107 patients receiving parenteral nutrition (group PN,n=107) at the same time.The outcomes of FDGE between of two groups were compared.Results:The incidence of FDGE in group EEN was 1.4%(1/71).And the incidence of FDGE in group PN was 10.3%(11/107).There was significant difference between the group EEN and the group PN (P

Objective To study the prognosis significance of intratumoral microvascular density(IMD)and the expression of vascular endothelial growth factor(VEGF)in malignant mesothelioma.Methods The data of 79 cases were reviewed of malignant mesothelioma confirmed by histology,immunohistochemistry and telectron microscope.CD34 and the expression of VEGF were detected by Immunohistochemical method.Results The age of the patients with malignant mesothelioma ranged from 11 to 79 years.The peak incidence of malignant mesothelioma was at the range of 40-50 years.The sex ratio of male to female was 1.78∶1.The survival period of the 79 cases was between 5 and 160 months with the medial survival of 23 months.According to histological structure,these cases were divided into epithelioid type(51 cases),sarcomatous type(20 cases)and mixed type(8 cases).For the VEGF positive expression,epithelioid type was 26 cases(50.98%),sarcomatous type was 13 cases(65.00)and mixed type was 5 cases(62.50%).Based on the mean IMD,the patients with malignant mesothelioma were divided into two groups.The survival time of the patients in the higher IMD group was significantly shorter than those in the lower IMD group.The analysis,on the relation of VEGF expression to IMD,as well as survival time and histological subtype,showed that VEGF expression was positively correlated to IMD(P=0.02,r=0.330 2).A significant difference existed between the survival of VEGF positive expression and negative expression(P0.05).Conclusion The results suggest that the prognosis of malignant mesothelioma probably related with IMD and VEGF expression.The higher index of IMD and the higher expression of VEGF might imply a worse prognosis.

Objective To evaluate the accuracy,practical value and methods of Doppler tissue imaging(DTI) in localizing the ventricular emergence site of accessory atrioventricular pathways.Methods Forty patients with pre-excitation syndrome were examined by DTI before intracardiac electrophysiologic studies(EP) and radiofrequency catheter ablation(RFCA).The pre-excitation zone was determined as a zone of maximum acceleration with “DTI acceleration mode” at the time of the delta wave or before the onset of the QRS complex.At the same time,the contraction velocity was measured from subendocardium of corresponding myocardial segment by “pulsed wave Doppler imaging”.Results Assuming the results of EP and RFCA as a gold standard,the correct localization by DTI was 80% but was only 47.5% and 52.5% by eletrocardiographic algorithms( P