Objective To investigate the effects of electro-acupuncture (EA) on remyelination after sciatic nerve was transected in rats, so as to explore the possible mechanism of EA in treatment of peripheral nerve injury. Methods After transection of rat's sciatic nerve, the nerve regeneration chamber was built,then the rats were given EA stimulation at frequencies of 5 Hz and 100 Hz, respectively, and Solochrome Cyanine staining, HE staining of nerve fibers, immunohistochemical staining and semi-quantitative image analysis technique were employed to observe the remyelination of the nerve fibers and S-100 protein expression of Schwann cells. Results EA could significantly promote remyelination of the nerve fibers, elevate S-100 protein expression of Schwann Cells in repairing process of transected sciatic nerve of rats. The therapeutic effect of 5 Hz EA group was better than that of 100 Hz EA group. Conclusion EA is an effective way for the treatment of peripheral nerve injury. It can promote proliferation of Schwann cells and nerve fiber myelination. The therapeutic effects of EA are frequency-depondent.

Objective To explore the expression of drug transporter organic anion transporting polypeptide2(Oatp2) and P-glycoprotein(P-gp) in brains of chronic epileptic rats induced by lithium-pilocarpine.Methods Chronic epilepsy modles were elicited after status epilepticus(SE) induced by lithium-pilocarpine in adult Wistar rats.The expressions of Oatp2 and P-gp were detected by immuno-histochemistry(SABC) method.Results Positive expression of Oatp2 was predominantly in the plexus epithelial cells and(blood-brain) barrier endothelial cells.Weak staining for Oatp2 was also detected in the neurocyte.In contrast,apparent expression of(P-gp) was seen in brain capillary and neurocyte only.Compared with normal control rats,expression of Oatp2 on the plexus epithelial cells and brain capillary in chronic epileptic rats was significantly decreased(P

Objective To explore an assessment questionnaire for assessing caregiving knowledge of primary caregivers in children with nephrotic syndrome. Methods Delphi method was used to review the literature. Ten primary caregivers of children with nephrotic syndrome were interviewed and five medical, nursing experts were pre-surveyed to form an expert consultation questionnaire. Sixteen experts from some provinces were selected to carry out two rounds expert consultation. To screen the dimensions, factors, entries of the questionnaire and to analyze the reliability and validity of the questionnaire. Results The positive coefficient was 100%, the expert authority coefficient was 0.95 and the expert co-ordination coefficient W test was P<0.05 of the two rounds expert consultation. The questionnaire data included 6 dimensions, 26 factors, 106 items. The basic knowledge of disease, diet knowledge, medication knowledge, personal hygiene and infection prevention, activity and rest, home care constitute the dimensions. The Cronbach α coefficient was 0.872, the test-retest reliability was 0.823, the split-half reliability was 0.855, and the average overall validity index of the questionnaire was 0.948. Six common factors were extracted for exploratory factor analysis and the cumulative variance contribution> 65.633%. Conclusions The questionnaire of caregiving knowledge of primary caregivers of children with nephrotic syndrome developed by Delphi method has high reliability. The questionnaire is of practical significance for evaluating the care of primary caregivers of children with nephrotic syndrome.

Objectives To explore the long-term prognosis, treatment (especially dietary treatment) of classic maple syrup urine disease. Methods The complications and dietary treatment were observed by follow-up study of a classic MSUD patient. Results The patient have obvious damage in nervous system. However, reasonable dietary leucine tolerance therapy after the neonatal period can effectively reduce the metabolic crisis and complications. A mutation in BCKDHB gene was detected in the patient by genetic testing. Conclusion It is suggested that dietary restriction and monitoring of branched-chain amino acids are helpful to reduce the development of acute metabolic crisis and complications and improve the quality of life.

Objective To investigate the effect of nicergoline on cognitive ability and executive function for patients with vascular cognitive impairment without dementia (VCIND).Methods Totally 68 patients with VCIND were divided into conventional treatment group (33 cases) and nicergoline treatment group (35 cases,nicergoline 10 mg,tid added on basal conventional treatment).All of cases were continuously treated for 12 weeks.Cognitive ability and executive function before and after treatments were evaluated.Results Test scores of immediate free recall of a list of words,the numbers and symbols transformation,the word fluency,clocks and watches painting,and minimental state examination (MMSE) were significantly higher in nicergoline treatment group than in conventional treatment group (P＜0.05).Conclusions Nicergoline may improve attention and memory as well as cognitive ability in patients with vascular cognitive impairment without dementia.

Objective To explore the electrode fixing method in children long-term electroencephalography and to improve the traditional method by combining with the pediatric characteristics and by means of clinical practice .Methods The pediatric subjects were given the appropriate preinstallation preparation ,then the double-layer elastic bandage combined with adhesive tape fixing method was adopted to fix the electrodes .Results The new method was generally accepted by children subjects ,the electrodes were easily to be installed and demounted without toxicity and residue ,the record diagram was better in quality .Conclusion Compared with the traditional method ,the improved electrode fixing method has the advantages of simplicity ,rapidness ,safety ,stability and humanization ,and is worth popularizing .

Along with the gradual implementation in legislative work of traditional Chinese medicine,the acupuncture education in Australia has rapidly developed.This paper will examine the history of acupuncture in Australia,laws and regulations of acupuncture education in Australia and the Australian acupuncture education system.It will also demonstrate on how the legislation of traditional Chinese medicine has promoted the development of acupuncture education in Australia.

Adolescent ; Adult ; Aged ; Child ; Female ; Foreign Bodies ; diagnostic imaging ; Head ; Humans ; Male ; Middle Aged ; Neck ; Tomography, Spiral Computed ; Tomography, X-Ray Computed ; Young Adult

Objective To explore the effects of two air sampling methods including natural precipitation method and impacting method for detecting the bacterial count of indoor air of workplace of cosmetic plants. Methods The diameters of 44 glass bacteria-culture plates for those two sampling methods were measured. The indoor air of workplace of cosmetic plants were sampled by those two sampling methods simultaneously. The natural precipitation method was performed for 5- minute exposure ,the impacting method was performed by MAS-100 airborned bacteria sampler at flow rate of 100 L/min for 30 s,2 min,8 min respectively.All of the data on the bacterial counts of air obtained from various sampling ways were statistically analyzed.The measures for quality control of air sampling progress were put forward also. Results The diameters of 44 glass bacteria-culture plates were 8.39-8.70 cm,which were lower than the standard(9 cm?雪. The bacterial counts of air samples collected by natural precipitation method at the same location showed higher coefficient of variation,higher median,lower qualified rate compared with those by impacting method. The bacterial counts of air decreased,when the impacting sampling method was performed for 8 min continuously with sampling volume of 800 L. Conclusion The impacting method with advantages including mere influence from external environment and better precision could be primarily applied for air sampling in general condition,but it might show lower efficiency of collecting the airborne bacteria during the longer sampling period with higher sampling volume. The natural precipitation method with poor precision was suitable for longer term (8-30 min)air sampling in the relatively static environment with lower air flow and highly cleaned air. The bacterial counts of air obtained from natural precipitation method should be corrected if the diameters of glass bacteria-culture plates weren't meet the requirement of the national standard (9 cm?雪.

OBJECTIVE: To evaluate the clinical outcomes of medical supporting bone graft following posterior approach and bone cement implantation in the hip joint in treating intertrochanteric fracture.METHODS: A computer-based online search of Science Direct, Ei databases was performed for English articles published between January 1960 and October 2009, with the key words "bone cement, intertrochanteric fracture". In addition, CNKI and CBM were searched for related Chinese articles published between January 1994 and October 2009, with the key words "intertrochanteric fracture, coxa vara, posterior approach of hip joint, bone cement implantation in major screw hole". Moreover,related books were manually searched. Treatment of intertrochanteric fracture, basic and clinical experiment of intertrochanteric fracture treated by bone cement was included.RESULTS: Intertrochanteric fracture treatment includes lateral or anterior approach for dynamic hip plate system, dynamic hip screw, and femoral proximal intramedullary screw internal fixation. Dynamic hip screw is standard internal fixation to treat intertrochanteric fracture, with strong anti-rotation function, and meets the biomechanical requirements. However, screw loosening,extraction and breakage frequently occur. Cancellous bone screw track enhanced by bone cement increases screw retention force,benefits screw compression, enhances bone-screw interface to transfer the stress to screw-bone regions, which significantly increases the anti-flexion and anti-torsion strength of dynamic hip screw and improves fracture stability. The mechanism involves the integral formation of cancellous bone, bone cement and screw by the micro-interlocking of bone trabecula and surrounding cancellous bone.CONCLUSION: Dynamic hip screw filled by bone cement significantly enhances the anti-flexion and anti-torsion strength of internal fixation and improves fracture stability.