2.Tumor lysis syndrome in solid tumors
Zhantao XIE ; Bo Qü ; Long GUO ; Yi Lü
Tumor 2010;(4):352-355
Tumor lysis syndrome (TLS) occurs frequently in the chemotherapy of patients with hematologic malignancies; however, it is rarely reported in solid tumors. Because of the latent incidence, TLS is vulnerable to misdiagnosis or missed diagnosis, leading to a poor prognosis. TLS is characterized by hyperuricemia, hyperkalemia, hyperphosphatemia and hypocalcaemia, with some major complications such as acute renal failure and cardiac arrhythmias. Therefore,the key treatment strategies usually refer to appropriate prophylactic measures for high-risk patients, early diagnosis and aggressive therapy. This paper reviews 87 cases of TLS reported in the English literature and discusses its incidence, prevention and treatment.
3.Mechanisms and function of angiopoietin.
Shu LIU ; Lü-ya WANG ; Heng-yi GUO ; Qi-xia WU
Chinese Journal of Pathology 2003;32(3):273-275
4.Diagnosis and treatment strategy of the retroperitoneal fibrosis associated with hydronephrosis
Zhiwei WANG ; Guang SUN ; Zhanjun GUO ; Yi WANG ; Zheng Lü ; Zhenfeng ZHANG
Chinese Journal of Urology 2013;(2):101-104
Objective To summarize the diagnosis and treatment strategy of the retroperitoneal fibrosis(RPF)associated with hydronephrosis.Methods The clinical data of 26 RPF cases treated from Jan.2005 to Mar.2012 were analyzed retrospectively.Early symptoms mainly included lumbar,flank,abdominal pain,nausea and vomit.Retroperitoneal mass was found in 12(46.2%)cases by ultrasonography,while in 23(88.5%)cases by CT.Results Ureterolysis with intra-peritoneal transposition was underwent in 10 cases who were followed up for 6-25 months,and no relapse was found.Ureterocystostomy was underwent in 1 cases for difficulty in ureterolysis who was followed up for 45 months,and no relapse was found.D-J stent inter-ureter drainage was performed in 15 cases,all of whom had replaced the D-J stent discontinuously except that 2 cases had ceased replacement successfully,and all of the obstruction were relieved during the follow-up period for 16-84 months post-operatively.Conclusions Retroperitoneal mass can be found by CT of abdomen effectively.The therapeutics should depend on the pathological condition of the retroperitoneal mass.Obstruction can be relieved effectively by both ureterolysis with intraperitoneal transposition and D-J stent inter-ureter drainage and replacement.The complication occurred in the replacement of D-J can be relieved or eliminated by all kinds of measures.The unimpaired kidney drainage should be paid attention in the follow-up.
5.Proliferation inhibition effect by silencing PLCε in the bladder cancer cell lineBIU-87
Yongcan GUO ; Chunli LUO ; Xiaozhong CAI ; Jianhong XIE ; Liping OU ; Yi ZHAO ; Chunfang Lü ; Huiying JI ; Xiaohou WU
Chinese Journal of Urology 2010;31(7):467-470
Objective To study the proliferation inhibition effect by silencing PLCε gene expression with RNA interference in BIU-87 cells. Methods The specific short hairpin RNA recombinant plasmids were constructed by gene clone technology.The expression level of PLCε protein and mRNA were detected by Western blot and RT-PCR respectively after transfected recombinant plasmids into BIU-87 cells.The influence on proliferation was check by MTT.The changes of proliferating cell nuclear antigen(PCNA)were analyzed by immunocytochemical method,and the distribution of cell cycle was analyzed using flow cytometry. Results After transfected with the specific recombinant plasmids,PCNA expression was decreased 33.08%,and the analysis of cell cycle indicated that cells of G0/G1 phase were increased comparision with(40.75±2.30)%and(40.00±1.76)0A,and its G2/M phase cells(8.16±0.51)%were decreased strikingly compared with group control(31.20±1.76)%and group NP(35.94±1.58)%.Cells were blocked at G0/G1 phase,the cell proliferation was inhibited obviously. Conclusion PLCε may play an important role in proliferation of bladder cancer cells,which could be a potential target of biological treatment on bladder cancer in the future.
6.Identification of constituents in vitro and blood-absorbed ingredients of protective effect on acute liver injury from Yin Chen Hao decoction based on UPLC-QTOF/MS
Yi-qing YAO ; Qi CAO ; Xuan WANG ; Hui-lin MA ; Yu-miao CHEN ; Si-yi ZHAO ; Min-xuan GUO ; Jia-meng HU ; Dong-yao WANG ; Di-ya LÜ
Acta Pharmaceutica Sinica 2023;58(5):1173-1180
To identify the active constituents
7.Role of hepatitis B virus infection in pathogenesis of IgA nephropathy.
Nian-song WANG ; Zhao-long WU ; Yue-e ZHANG ; Mu-yi GUO ; Lü-tan LIAO
Chinese Journal of Preventive Medicine 2003;37(1):37-40
OBJECTIVETo clarify the relationship between hepatitis B virus (HBV) infection and IgA nephropathy (IgAN).
METHODSHBV antigen (HBAg) in renal tissues of the patients with IgAN was detected by immunohistochemical technique, the carrier status and localization of HBV DNA in renal tissues were determined by Southern blot analysis and in situ hybridization.
RESULTSSerum HBsAg was detected in 18 of the 100 patients with IgAN (18%), HBAg was detected in 31 of 100 patients (31%) in their renal tissue and in 20 of 31 patients (65%) in their glomeruli, and both HBsAg and HBcAg were detected in 10 of 31 patients (32%), respectively. HBcAg was also found in tubular epithelia (45%, 14/31) and renal interstitium (6%, 2/31), respectively. Five of six cases were proved to be positive of integrated-form HBV DNA in their renal tissue by Southern blot analysis. In situ hybridization demonstrated that HBV DNA was 8/8 and 6/8 positive in their renal tubules and glomeruli of all eight specimens, localized in the nucleus of tubular epithelial cells, glomerular mesangial cells, as well as infiltrated interstitial lymphocytes.
CONCLUSIONHBV infection closely related with IgAN and HBV infection might be involved in pathogenesis of IgAN.
Adolescent ; Adult ; Female ; Glomerulonephritis, IGA ; complications ; virology ; Hepatitis B ; complications ; Hepatitis B Antigens ; analysis ; Hepatitis B virus ; isolation & purification ; Humans ; Male ; Middle Aged
8.A new chromone glycoside from Cassia siamea lam.
Tai-sheng LÜ ; Yang-hua YI ; Hong-ying YUAN ; Zhi-guo ZHANG ; Wei LIU
Acta Pharmaceutica Sinica 2003;38(2):113-115
AIMTo study the chemical constituents of the stem of Cassia siamea.
METHODSThe compounds were isolated by chromatography on silica gel, and identified on the basis of spectral analysis.
RESULTSFive compounds were isolated and identified as: beta-sitosterol (I), sucrose (II), n-octacosanol (III), 2-methyl-5-(2'-hydroxypropyl)-7-hydroxy-chromone-2'-O-beta-D-glucopyranoside (IV) and piceatannol (V).
CONCLUSIONCompound IV is a new compound. Compounds II, III and V were obtained from this plant for the first time.
Cassia ; chemistry ; Chromones ; chemistry ; isolation & purification ; Fatty Alcohols ; chemistry ; isolation & purification ; Monosaccharides ; chemistry ; isolation & purification ; Plant Stems ; chemistry ; Plants, Medicinal ; chemistry ; Stilbenes ; chemistry ; isolation & purification
9.An investigation on failure mechanisms of primary 327 hip arthroplasties.
Sheng-Jun GUO ; Yi-Xin ZHOU ; Liang ZHANG ; Jian WU ; Ming LÜ ; Jing TANG ; Li-Ang ZHANG
Chinese Journal of Surgery 2009;47(3):168-171
OBJECTIVETo summarize the detailed failure mechanisms of revision hip arthroplasties and related risk factors.
METHODSFrom November 1988 to July 2008 revision of total hip arthroplasties was performed in 327 patients. The medical history, clinical and imaging material and operation records were investigated.
RESULTSRegarding revision as the end point of the study, the reasons for 327 revision arthroplasties were aseptic loosening in 226 hips (69.1%), infection in 52 hips (15.9%), periprosthetic fracture in 22 hips (6.7%), instability in 17 hips (5.2%), stem fracture in 5 hips (1.5%) and liner dissociation in 5 hips (1.5%).
CONCLUSIONSThe main failure mechanisms of primary hip arthroplasties are aseptic loosening and infection of implants, which could be attributed to improper selection of operation indications and implants and limitations to surgical philosophy and technique.
Adult ; Aged ; Aged, 80 and over ; Arthroplasty, Replacement, Hip ; adverse effects ; Female ; Humans ; Male ; Middle Aged ; Periprosthetic Fractures ; Prosthesis Failure ; Reoperation ; Retrospective Studies ; Risk Factors ; Surgical Wound Infection ; Treatment Failure
10.Targeted magnetic resonance imaging with intraperitoneal and intravenous streptavidin (SA)-DTPA-Gd: a comparative study in tumor-bearing nude mice.
Jing YE ; Yi-kai XU ; Xian LIU ; Guo-shi LÜ
Journal of Southern Medical University 2006;26(2):139-143
OBJECTIVETo investigate the effect of streptavidin (SA)-DTPA-Gd after intraperitoneal and intravenous administration for tumor enhancement in targeted magnetic resonance imaging (MRI).
METHODSBiotinylated monoclonal antibody CL3 (600 microg) was intravenously injected into 12 BALB/c nude mice with subcutaneous inoculation of LoVo cells, followed by administration of 80 microg avidin as the chaser 24 h later and then SA-DTPA-Gd was injected intravenously or intraperitoneally after another 30 min. MRI was performed before and 20, 60 min and 3, 6, 9, 12 h after the injection of the contrast agents, and the MR signal intensity of the tumor and liver was determined.
RESULTThe maximum enhancement ratio of the tumor was 70.2% in the intravenous injection group and 46.4% in the intraperitoneal group, showing significant difference between them. The maximal enhancement rate of the liver was 23.7% in the intraperitoneal group and 20.4% in the intravenous group, showing no significant difference.
CONCLUSIONMR targeted imaging with biotinylated monoclonal antibody CL3 and SA-DTPA-Gd has specific enhancement effect. Higher blood level of SA-DTPA-Gd in the intravenous group facilitates the tumor enhancement in MRI in subcutaneous tumor model.
Adenocarcinoma ; diagnosis ; metabolism ; Animals ; Antibodies, Monoclonal ; administration & dosage ; pharmacokinetics ; Cell Line, Tumor ; Colorectal Neoplasms ; diagnosis ; metabolism ; Contrast Media ; administration & dosage ; Gadolinium DTPA ; administration & dosage ; pharmacokinetics ; Humans ; Injections, Intraperitoneal ; Injections, Intravenous ; Magnetic Resonance Imaging ; methods ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasms, Experimental ; diagnosis ; metabolism ; Reproducibility of Results ; Sensitivity and Specificity ; Streptavidin ; administration & dosage ; pharmacokinetics ; Transplantation, Heterologous