1.Estimation of Internal Irradiation Absorbed Dose for Nanometer-iron Nuclide Therapy of Liver Cancer
Jin OU ; Ping XIONG ; Ping GUO
Chinese Journal of Medical Physics 2010;27(1):1662-1664,1671
Objective :To study the two common method of internal irradiation for nanometer-iron nuclide,and how to estimation of the absorbed dose for each of them.Methods:We chose the nanometer-iron nuclide emitting β-ray during the decay period,Respectively,Arterial injected into the liver or percutaneous puncture direct inject into the liver.Results:Through the formula,a few nanometer-iron nuclide can produced greater radiation.Conclusion:The estimation of absorbed dose depends on the therapy method.
2.A Theoretical Model for Targeting Therapy With the Particles of the Paramagnetic Nanometers-iron Nuclide Guided by Outside Magnetic Field
Ping GUO ; Ping XIONG ; Changqing GUO ; You CHEN ; Jishan HE
Chinese Journal of Medical Physics 2006;23(6):458-461
A theoretical model for the magnetic forces on ferromagnetic drug particles in a guiding magnetic field is established with the consideration of the electromagnetic field theory. Our model shows that the ferromagnetic drug particles can concentrate on the targeted area with the guidance of the outside magnetic field. Both the distribution of the intensity of the guiding magnetic field and the forces on ferromagnetic drug particles are studied numerically. The theoretical results are in good agreement with the experimental results reported recently.
3.Allele frequencies of 5 short tandem repeat loci of Kashin-Beck disease patients on chromosome 12
Zhi-guang, PING ; Li, LIU ; Xiong, GUO
Chinese Journal of Endemiology 2008;27(5):475-478
Objective To analyze the allele frequencies of 5 short tandem repeat(STR)loci(D12S313,D12S304,D12S1640,D12S1708 and D12S1583)on chromosome 12 among Kashin-Beck disease(KBD)patients and the control population living in the area suffered from KBD.Methods Fifty KBD patient8 and 50 non-KBD patients were chosen in endemic afea of Shaanxi Province,5 STR loci on chromosome 12 were genotyped by the technology of polymerase chain reacfion(PCR)and capillary electmphoresis.The pelymorphisms of STR in these popIllations were analyzed.The allele and genotype frequencies of each STR in the corresponding groups were caleulated and compared. Results In KBD group,the 5 STR loci had 8,6,7,5 and 11 types ofalleles and 17,11,15,8 and 28 genotypes, respectively;while in the control group,the number of aUele types of 5 STR loci were 6,8,6,4 and 10,the number of genotype of those loci were 13,21,14,8 and 23,respectively The allele frequence of D12S304 locus was statiBtically significant between KBD patients and controls(P<0.05),especially for the 319 bp allele(P<0.006 25). Conclusion There is an association between D12S304 locus and KBD.The 319 bp allele might play the key role.
4.Identification of Scutellaria amoena CH Wright and Scutellaria baicalensis Georgi with HPLC Finger-prints
Chunmei XIONG ; Ping ZHANG ; Yadong GUO
China Pharmacy 2007;0(33):-
OBJECTIVE:To identify different origin of Scutellaria amoena CH Wright and Scutellaria baicalensis Georgi indexed in China Pharmacopeia by HPLC fingerprints.METHODS:RP-HPLC method was adopted in which the samples were separated on Zorbax SB-C18(250mm?4.6mm,5?m)column at a column temperature of 30℃.The gradient elution was performed on the mobile phase which consisted of acetonitrile(contairing 1.0% methanoic acid)-water(containing 1.0% methanoic acid)at a flow rate of 1.0mL?min-1.The detection wavelength was set at 274nm.RESULTS:The fingerprints of Scutellaria amoena CH Wright that from different places were of great resemblance,so were the fingerprints between Scutellaria baicalensis Georgi that from different places,but the fingerprints between Scutellaria amoena CH Wright and Scutellaria baicalensis Georgi were of significant difference,with resemblance of only 0.859.CONCLUSION:The RP-HPLC fingerprint serves as basis for the further study of the quality criteria of Scutellaria amoena CH Wright and Scutellaria baicalensis Georgi.
5.Thirty cases of knee osteoarthritis treated by acupuncture and warming needle moxibustion.
Guo-ping XIONG ; Qin HUANG ; Yuan-feng DONG
Chinese Acupuncture & Moxibustion 2011;31(6):551-552
Acupuncture Therapy
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Adult
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Aged
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Female
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Humans
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Male
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Middle Aged
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Moxibustion
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Osteoarthritis, Knee
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therapy
7.Optimization of Fermentation Medium for Honggumycin Using Response Surface Methodology
Zhi-Qiang XIONG ; Ping XU ; Guo-Quan TU ;
Microbiology 1992;0(04):-
Under the shaking-flask culture, fermentation medium of honggumycin produced by Streptomyces 702 were studied.The experiment was used response surface methodology to optimize the shaking-flask fermentation medium.Firstly, we applied full factorial design to screen important factors soybean meal and industrial peptone which affected hongmycin produced by Streptomyces 702.Furthermore, we designed experiment to obtain the steepest ascent path and optimal level by the central composite design.The optimum medium consisted of (g/L): maize starch 20, maize meal 20, glucose 20, soybean meal 23, industrial peptone 9, KNO_ 3 2.5, (NH_ 4 )_ 2 SO_ 4 2.5 KH_ 2 PO_ 4 0.3, NaCl 3, CaCO_ 3 6, bean oil 5mL/L.Under the optimal medium, the yield of honggumycin was up to 1500 g/mL, which was increased by 308% than the original medium.
8.The relationship between the postmortem interval and growing of the fly.
Ping XIONG ; Ping GUO ; Qiao ZENG
Journal of Forensic Medicine 2002;18(2):71-73
OBJECTIVE:
To study the growing of flies on body after different postmortem interval.
METHODS:
In natural environment, time points when flies coming, gathering, making eggs, and maggots pupating, emergencing were observed on a female body died of disease.
RESULTS:
The time when flies coming, gathering, making eggs, and maggots pupating, changing to chrysalis, and emergencing were 0.2, 28, 44, 60, 112 and 248 h after one's death, respectively.
CONCLUSION
This study may be used in PMI estimation.
Animals
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Diptera/growth & development*
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Female
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Humans
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Larva/growth & development*
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Ovum/growth & development*
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Postmortem Changes
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Pupa
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Temperature
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Time Factors
9.Inhibitory effect of pyridoxine hydrochloride combined with chemotherapeutics on mice hepatoma cell line H22
Ping JIANG ; Xueyan CHEN ; Fang GUO ; Zhongning ZHU ; Chen XIONG ; Suhua QIU
Cancer Research and Clinic 2016;28(12):797-801
Objective To observe the inhibitory effect of pyridoxine hydrochloride (PN) combined with common chemotherapeutics on mice hepatoma cells H22 in vitro. Methods MTT assay was used to determine the effects of PN in combination with 10 different antineoplastic agents on H22 cells, and immuno-histochemistry was used to observe the distribution of PN in H22 cells and morphologic changes of the cells before and after PN treatment. Results After 24 hours incubation with 5 mmol/L PN, the treated cells expanded apparently with nucleus chipping. PN entered the tumor cell and was mainly condensed in cytoplasma and H22 cells were sensitive to PN. When administered concomitantly with chemotherapic agents, most of the combinations showed antagonistic effects while a few of the combinations were additive. For instance, doxorubicin (ADM) used in combination with PN inhibited cell proliferation with an IR value (IR=0.63) much lower than ADM alone (IR=0.71, P<0.01), and the CI value was less than 0.9, which indicated an antagonistic effect. However, PN in combination with ifosfamide (ICTX) showed additive effect (CI>0.9), and the IR value (IR=0.60) in combined group was higher than that (IR=0.40) in ICTX group (P<0.05). Conclusion PN treatment could increase the intracellular PLP level and result in growth inhibition and cell death, and combined administration of PN and ICTX might be a potential method to improve efficacy and to reduce toxic effects while a co-administration of PN and ADM should be avoided.
10.Quantitative Analysis of Immuno-fluorescence of Nuclear Factor-κB Activation.
Min XIU ; Feng HE ; Yuanlei LOU ; Lu XU ; Xiong JIEQI ; Ping WANG ; Sisun LIU ; Fei GUO
Journal of Biomedical Engineering 2015;32(3):669-674
Immuno-fluorescence technique can qualitatively determine certain nuclear translocation, of which NF-κB/ p65 implicates the activation of NF-κB signal pathways. Immuno-fluorescence analysis software with independent property rights is able to quantitatively analyze dynamic location of NF-κB/p65 by computing relative fluorescence units in nuclei and cytoplasm. We verified the quantitative analysis by Western Blot. When we applied the software to analysis of nuclear translocation in lipopolysaccharide (LPS) induced (0. 5 h, 1 h, 2 h, 4 h) primary human umbilical vein endothelial cells (HUVECs) , we found that nuclear translocation peak showed up at 2h as with calculated Western blot verification results, indicating that the inventive immuno-fluorescence analysis software can be applied to the quantitative analysis of immuno-fluorescence.
Active Transport, Cell Nucleus
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Cell Nucleus
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metabolism
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Cytoplasm
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metabolism
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Fluorescent Antibody Technique
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Human Umbilical Vein Endothelial Cells
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Humans
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NF-kappa B p50 Subunit
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metabolism
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Software