Objective To investigate the effects of paeoniflorin on proliferation and the protein expression of HFCL of human bone marrow stromal cells and to discuss molecular mechanism of blood enriching functions of paeoniflorin. Methods Flow cytometry and proteomics were used respectively to measure the effect of paeoniflorin on cell cycle and protein expression of HFCL. Results Paeoniflorin could promote HFCL from G_0/G_1 phase to S phase, increase proliferative index, up-regulate nine kinds of proteins, and down-regulate five kinds of proteins of HFCL. The proteins up-regulated include Ras-related nuclear protein, lamin A/C, isocitrate dehydrogenase 3 (NAD+), triosephosphate isomerase, ATP synthase, ribosomal protein P2, and chaperonin containing t-complex polypeptide 1. Conclusion Through promoting HFCL proliferation, acting on multiple protein targets, enhancing the synthesis of cytoarchitecture proteins and the expression of protein chaperonin, increasing the energy metabolism of HFCL, and suppressing the apoptosis of HFCL, paeoniflorin plays indirectly blood enriching function.

OBJECTIVE To detect the effect of AcasinTM dressing on surgical incision healing.METHODS Eighty cases with types Ⅰand Ⅱsurgical incision operation at outpatient clinic were divided into study group and control group.AcasinTM dressing was applied to study group while common aseptic dressing was applied to control group.The bacterial test results and the healing condition of the incision were observed.RESULTS The bacterial tests were negative in study group,the average healing time was 7.32?1.74 days,the healed rate of stage Ⅰ was 100.00%.The bacterial tests were positive in 10 cases of control group,10 cases had redness in incision,5 cases with drainage leakage,the average healing time was 10.12?2.24 days,The healed rate of stage Ⅰ was 90.00%.CONCLUSIONS AcasinTM dressing can eridicate pathogenic organism in surgical incision and suture,It can effectively prevent incision infection,and promote stage Ⅰincision healing.It is worth spread in clinic.

Objective: To study the effect of genetic polymorphisms of cytochrome P4501A1 (CYP1A1), N-acetyltransferase 2 (NAT2) on the susceptibility to renal cancer. Methods: The genetic polymorphisms of CYP1A1 and NAT2 were examined in the renal cancer patients and controls using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) methods. Results: The distribution of CYP1A1 (W/M) and NAT2 (intermediate) genotypes was significantly different between the renal cancer patients and controls (P<0.05). Individuals carrying CYP1A1 (W/M) or NAT2 (intermediate) genotypes had an increased risk for renal cancer(OR=2.487, 95% CI: 1.493-4. 142; OR=1.970, 95% CI: 1.128-3.442,respectively). Multivariate analysis showed increased risk for renal cancer patients carrying CYP1A1 (W/M) or NAT2 (intermediate) genotypes and those who smoke. Conclusion: The genotypes CYP1A1 (W/M) and NAT2 (intermediate) are the risk factors of renal cancer, and the 2 genotypes have a interactive effect and both have a joint effect with smoking.

Objective: To evaluate the relationship between the polymorphism of CYP1A1 gene and the genetic susceptibility to renal cancer. Methods: A case-control study was conducted in 175 renal cancer patients and 200 healthy control subjects to investigate the role of CYP1A1 gene A4889G(M2) and C4887A(M4) polymorphisms in renal cancer. PCR-RFLP was used to identify the genotypes of polymorphism. Results: The frequencies of A4889G and C4887A genotypes were not significantly different between RCC cases and healthy controls. M2/M2 genotype was positively correlated with the incidence of renal cancer (OR=2.225 [95%CI=1.134-4.365, P=0.020]). Conclusion: M2/M2 genotype of CYP1A1 gene may be a risk factor for renal cancer.

Animals ; Brain ; drug effects ; metabolism ; Brain Ischemia ; drug therapy ; metabolism ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Male ; Malondialdehyde ; metabolism ; Phytotherapy ; Rats ; Rats, Wistar ; Reperfusion Injury ; drug therapy ; metabolism ; Superoxide Dismutase ; metabolism

Objective To determine whether the ?_2-adrenoceptor genetic single nucleotide polymorphisms are associated with atrial fibrillation.Methods A case-control study was performed on 218 subjects,including 58 subjects with lone atrial fibrillation,50 subjects with atrial fibrillation and essential hypertension,50 subjects with essential hypertension and 60 healthy controls.The ?_2-adrenoceptor polymorphisms at amino acids 16 and 27 were identified by allele specific polymerase chain reaction(PCR).Results The Gly/Gly genotype as well as the Gly16 allele at amino acids 16 was significantly more common in the atrial fibrillation group than in the non-atrial fibrillation group.But there was no significant differenece in the allele frequency or the genotype frequency of the Gln27 Glu polymorphism between the two groups.In addition,the difference in the distribution of polymorphisms at amino acids 16 and 27 between the four different observed groups was not significant either.Conclusion Gly16 polymorphism of the ?_2-adrenoceptor overrepresenting in the atrial fibrillation group seems to be associated with atrial fibrillation.

Hyperhomocysteinemia is an independent risk factor for arterial and venous obstructive vascular di-seases. Raised plasma homocysteine concentrations are caused by genetic and non-genetic factors. The pathogenic mechanism is not clear. It may be associated with endothelium dysfunction, abnormal lipid metabolism, hypertrophy of intima media, increased platelet aggregation and several clotting factors interfered, etc. The metabolism and pathogenic mechanism of homocysteine are reviewed in this article, which provides theoretical foundation for the treatement of hyperhomocysteinemia.

0.05).During unilateral contact maximal biting EMG activity of the temporalis-anterior muscle on the bitting-side was higher than that on the disoccluded side(P0.05).Conclusion: Vertical distance occlusion contact may affect the EMG activity of temporalis-anterior muscle but not on that of masseter muscle during the central biting.

Objective To study the rationality of biochemistry teaching of liberal arts students and science students in one class and further to improve the quality of teaching.Methods A total of 138 students of seven-year traditional Chinese medicine of Grade 2013 as the objects of study,among whom there were 58 liberal arts students and 80 science students.Questionnaire were surveyed at the end of the teaching of biochemistry,including evaluation of the teaching effect and teaching reform.138 valid questionnaires were dispatched and recovered.And at the same time unified examination was carried out to both liberal arts students and science students.The t test,chi square test or the exact probability method were used to compare the data of two types of students with SPSS 19.0.Results Questionnaire survey:31.03％ (18/58) arts students and 27.50％ (22/80) science students felt that the class hours were not enough.48.28％ (28/58) arts students thought the weak chemical base to be the main factor affecting learning,and 50.00％ (40/80) science students thought that lack of interest was the most important factor.53.45％ (31/58) arts and 47.50％ (38/80) science students would agree that a metabolic reaction of the material was the most difficult problem.51.72％ (30/58) arts students and 42.50％ (34/80) science students preferred blackboard-writing.Test scores:arts and science students got average grades (70.81 ± 9.71) and (77.05 ± 8.46) respectively,and the difference was statistically significant (t=-4.02,P=0.000).Conclusions Survey results and analysis of test scores shows that teaching of liberal arts students and science students in one class is feasible.However,it is necessary to strengthen the students' chemical basis,improve their learning interest and optimize their learning methods,so as to realize the harmonious development of the students of arts and science,and to improve the overall teaching level.

Humans ; Oral Medicine ; Proteomics