7.5). Overall,the research demonstrates the effect of environmental parameters (temperature and carbon source) on AHL profile production by food-derived Pseudomonas. It lays the foundation for further investigation to elucidate the relationship between quorum sensing and food spoilage,and for the development of new food preservative by blocking the quorum sensing of food spoilage bacteria.

Three Gram-negative bacteria from commercial fresh fish were identified as Pseudomonas sp.,an important food spoilage bacteria, on the basis of 16S rDNA sequences.N-aeyl-homoserine lactones (AHLs) were the important quorum sensing molecules and regulated the expression of many characteristics in a cell-density-dependent manner in Gram-negative bacteria.The AHL biosensor detection revealed that three isolates produced AHLs molecules and strains FML05-1 and FML05-2 produced two types of AHLs at least and the main signal mole- cules were N-3-oxo-octanoyl-homoserine lactone(N-3-oxo-C_8-AHL).ALso the amount of AHL in FML05-2 reached to the maximum at 12h throughout growth.This was first study of quorum sensing signal molecule AHLs produced by the pseudomonas from food and layed the foun- dation for new strategies of food preservation based on interfering in quorum sensing of spoilage bacteria.

Objective To observe the effect of combined therapy of external point application and herbal concentrate-granules on patients with asthma (hot wheezing in TCM).Methods 60 patients suffering from onset period of bronchial asthma were randomized to a control group and a treatment group.The control group was given budesonide inhaler and theophylline sustained release tablets.The treatment group was given combined therapy of external point application and herbal concentrate-granules.The course of treatment was 10 days.To observe the value ofFEV1％,ACT scores and TCM syndrome scores etc.Results After treatment,the value of FEV 1％ [treatment group:(82.83 ± 11.35) ％,control group:(83.85 ± 16.72) ％] and ACT [treatment group:(19.86±2.32),control group:(19.66±2.54)] in both groups were markedly increased with statistical significance (P＜0.01); and the difference between the two groups were also significant; TCM syndrome scoring [treatment group:(4.27±3.65),control group:(5.05±4.14)] was notably decreased in both groups compared with the values before the treatment with statistical significance (P＜0.01); but the difference between the two groups was not significant (P＞0.05).Conclusion The combined therapy of external point application and herbal concentrate-granules can treat bronchial asthma in the acute clinical course and improve lung function.

12 strains of H2-producing bacteria were isolated and purified from anaerobic sludge, aerobic sludge and river bottom sludge by Hungate method. A new species of high efficient hydrogen production bacterium Enterococcus sp. LG1 (registration number: EU258743 ) was studied deeply. It was showed that the Enterococcus sp. LG1 was an anaerobic and Gram-negative bacterium. Sequence analysis of this type of clones showed that it was affiliated with the genus Enterococcus and it was not reported yet in other paper at present. Meanwhile, batch tests of anaerobic fermentative hydrogen production by Enterococcus sp. LG1 were investigated by using sterilization pretreated sludge as substrate. The changes of soluble COD, protein, carbohydrate and pH value during hydrogen fermentation were monitored. It was found that only hydrogen and carbon dioxide were produced by this strain and no methane was detected during fermentation. The maximal hydrogen yield was 36.48 mL/g TCOD and the hydrogen concentration in the gas phase was 73.5%. The Enterococcus sp. LG1 was a butyrate fermentation bacteria analyzed by metabolites.

After target blood glucose (HbA1C,fasting plasma glucose),serum lipid profiles (total cholesterol,triglyeeride) and control of blood pressure were achieved in 532 patients with type 2 diabetes mellitus, the level of serum uric acid was significantly decreased and the number of case of hyperuricacidemia also reduced (all P<0.01).

Objective To investigate the relationship between serum levels of adiponectin (APN) and retinol binding protein 4 (RBP4) in newly diagnosed type 2 diabetes mellitus (T2DM) patients. Methods 95 newly diagnosed T2DM patients and 55 non-diabetic volunteers from health examination population were studied. The serum concentrations of APN and RBP4 were determined by ELISA. The levels of plasma glucose, lipid profiles and insulin were also measured in fasting samples. Insulin sensitivity was assessed by insulin resistance index (HOMA-IR). The control group and diabetic group were divided into ≥60 years subgroup and <60 years subgroup respectively. According to the guideline designed by Chinese Diabetes Society(CDS)in 2004, the control group and diabetic group were divided into different kinds of abnormal metabolism groups. Results (1)The level of APN was significantly decreased in diabetic group compared with the control group[(7.26±4.69)mg/L vs. (11.93±4.89)mg/L], while the serum level of RBP4 in diabetic group was obviously higher than that in control group[(16.48±7.82) mg/L vs. (10.91±5.26) rag/L]. (2) The prevalence of metabolic syndrome in diabetic group was higher than that in control group (46.3% vs. 7.3%). (3)APN level was decreased with the increasing number of components of MS, and RBP4 level was increased with the increasing number of components of MS. (4) In DM group, APN level was correlated negatively with MS, waist circumference and BMI, and RBP4 level was correlated positively with waist circumference, HOMA-IR, BMI and TG. Conclusions APN and RBP4 levels are significantly associated with metabolic syndrome. Hypoadiponectinemia and higher retinal binding protein 4 may play an important role in the pathogenesis of MS.

Objective To investigate the correlation between 25-hydroxyvitamin D [ 25 ( OH ) D ] and indicators of glucose metabolism in inpatients with type 2 diabetes mellitus .Method We retrospectively ana-lyzed the clinical records of 214 inpatients with type 2 diabetes mellitus , including age , body mass index (BMI), systolic blood pressure, diastolic blood pressure, and laboratory test results such as serum parathyroid hormone (PTH), 25(OH)D, creatinine, calcium, phosphorus, glycosylated hemoglobin A1c (HbA1c), fast-ing blood glucose , and fasting insulin .Results The prevalence of vitamin D deficiency was high in these pa-tients, with 47.2%of them having a serum 25(OH)D concentration lower than 10 ng/ml.Using 25(OH)D level less than 10 ng/ml as the cut-off point, the patients were divided into vitamin D severe deficiency (Vit-SD) group and vitamin D non-severe deficiency (Vit-NSD) group.Compared with the Vit-NSD group, the Vit-SD group had younger age [ (55.27 ±13.71) years vs.(60.76 ±12.32) years, P=0.001] and higher HbA1c level [ (9.00 ±2.01)% vs.(8.45 ±1.86)%, P=0.025].BMI [ (25.09 ±4.01) kg/m2 vs.(25.39 ± 3.53) kg/m2, P=0.523], fasting blood glucose [ (8.91 ±3.31) mmol/L vs.(8.16 ±3.02) mmol/L, P=0.063], fasting serum insulin [ (21.32 ±32.50) mIU/L vs.(21.92 ±26.95) mIU/L, P=0.873] and homeostasis model assessment of insulin resistance index (97.60 ±8.92 vs.7.53 ±9.39, P=0.954) in these two groups were with no statistically significant difference .The association analyses showed that HbA 1c was sig-nificantly negatively correlated with 25 ( OH ) D ( r =-0.190 , P=0.003 ) and not correlated with serum PTH, calcium, or phosphorus , after adjusting for age and creatinine .Multiple linear regression revealed that HbA1c had a significantly negative correlation with age (β=-0.220, P=0.000) and 25 (OH) D (β=-0.184 , P=0.000 ) .Conclusions There is high prevalence of vitamin D deficiency in inpatients with type 2 diabetes mellitus, which is even higher in relatively young patients .Serum 25 (OH) D may be negatively correlated with HbA1c in these patients independently from age , BMI, fasting serum insulin, and homeostasis model assessment of insulin resistance index .

BACKGROUND:The risk of lower extremity deep venous thrombosis was high in patients with bone metastases. Major surgery is a major risk factor for thrombosis. There was no standard prophylactic regimen available.
OBJECTIVE:To investigate the efficacy and safety of low molecular weight heparins versus rivaroxaban in the postoperative prevention of lower extremity deep venous thrombosis in patients with bone metastases.
METHODS:From January 2010 to December 2013, a total of 73 patients with bone metastasis in spine, pelvis and lower extremities, who underwent open surgery in the Department of Musculoskeletal Tumor, Third Hospital, Hebei Medical University, China, were retrospectively analyzed. The patients were divided into low molecular weight heparins group (n=41) and rivaroxaban group (n=32) according to the prophylactic drugs after surgery.
RESULTS AND CONCLUSION:Nine cases (22%) in the low molecular weight heparins group were found lower extremity deep venous thrombosis, and six cases (19%) in the rivaroxaban group suffered from lower extremity deep venous thrombosis, showing no significant differences (χ2=0.11, P=0.74). The incidences of bleeding events in both groups were respectively 7.32%and 6.25%, showing no significant differences (correctionχ2=0.083, P>0.05). There were no significant differences regarding the levels of platelet, activated partial thromboplastin time and prothrombin time between both groups preoperatively or postoperatively (P>0.05). Therefore, the efficacy and safety of low molecular weight heparins and rivaroxaban in the postoperative prevention of lower extremity deep venous thrombosis were similar. Both could effectively reduce the incidence of deep venous thrombosis, with a relative low risk of bleeding.

Objective To observe the effect of treating asthma in remission stage(Latent phlegm in lung syndrome) withJinlong-Gubenmixture.Methods 108 patients of asthma on remission stage were randomized into a control group and a treatment group. The treatment group was givenJinLong-GuBen mixture. The control group was given nothing for treatment. Both groups were treated for 120 days and follow-up period were 360 days. The change of ACT scores and FEV1 was observed.Results After treatment, ACT scores(t=2.931, 2.618) and FEV1(t=2.011, 2.680) of treatment groups were markedly increased than before the treatment(P<0.05), and the effect of the treatment group was better than the control group(P<0.01). After treatment, the effective rate of treatment group was 93.75%(45/48), and the effective rate of control group was 78.72%(37/47),there existed statistical significance between the two groups(U=-0.4531,P<0.01). Conclusion The theory of latent phlegm in lung has a good guiding function in treating asthma in remission stage(Latent phlegm in lung syndrome).

〔Abstract〕 In the paper , a case study of Tongji Hospital of Tongji University is conducted and the construction method and the imple -mentation plan for the wireless network based on 802.11 ac standard are introduced .The implementation effect of WLAN based on 802.11 ac standard is summarized and analyzed .By comparing the old and the new standard , the construction plan and the future development of WLAN in hospitals are discussed .